Journal: Neural Regeneration Research

Article Title: Perfluoropentane-based oxygen-loaded nanodroplets reduce microglial activation through metabolic reprogramming

doi: 10.4103/NRR.NRR-D-23-01299

Figure Lengend Snippet: PFP-OLNDs treatment alleviates TH + neuron death in the MPTP model of PD. (A, B) Representative immunofluorescence staining for Iba1 (red, Cy3) in the substantia nigra 4 days after the last MPTP injection. In vivo intraperitoneal injections of MPTP in mice greatly increased the density of microglia in the substantia nigra region, and intranigral PFP-OLNDs injections prevented the MPTP-induced microglia activation. (C, D) Representative immunofluorescence staining for TH (green, Alexa Fluor 488) in the substantia nigra 4 days after the last MPTP injection. Intranigral PFP-OLNDs injections reduced TH + neuronal death induced by MPTP treatment. Scale bars: 100 μm (upper panel); 50 μm (lower panel). Data are expressed as mean ± SEM ( n = 6 mice per group). * P < 0.05, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by Bonferroni post hoc test). Iba1: Ionized calcium binding adaptor molecule 1; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; PD: Parkinson’s disease; PFP-OLNDs: perfluoropentane-based oxygen-loaded nanodroplets; TH: tyrosine hydroxylase.

Article Snippet: After washing, the slices were incubated for 1 hour at room temperature with Cy3-conjugated goat anti-rabbit IgG (1:300, Cat# GB21303, RRID AB_2861435, Servicebio, Wuhan, China) and goat anti-mouse IgG labeled with Alexa Fluor 488 (1:400, Cat# GB25301, RRID: AB_2904018, Servicebio).

Techniques: Immunofluorescence, Staining, Injection, In Vivo, Activation Assay, Binding Assay

Journal: Neural Regeneration Research

Article Title: Gamma-glutamyl transferase 5 overexpression in cerebrovascular endothelial cells improves brain pathology, cognition, and behavior in APP/PS1 mice

doi: 10.4103/NRR.NRR-D-23-01525

Figure Lengend Snippet: GGT5 overexpression in cerebrovascular endothelial cells alleviates Aβ-related pathology in APP/PS1 mice. (A) Representative immunofluorescence images of Aβ plaques in the mouse Ctx and Hip. No positive staining for Aβ plaques was observed in the Ctx and Hip of mice in the WT + Vector group and the WT + GGT5 group. However, there were abundant Aβ deposits in the brains of mice in the APP/PS1 + Vector group and the APP/PS1 + GGT5 group. Importantly, the APP/PS1 + GGT5 group exhibited a noticeable reduction in the number of insoluble Aβ plaques compared with the APP/PS1 + Vector group. DAPI, blue; 6E10, red (Cy3). Scale bar: 100 µm. (B, C) Histograms showing the percent area and number of 6E10-positive Aβ plaques in the Ctx (B) and Hip (C). Data are expressed as mean ± SEM ( n = 4 per group). * P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc test). Aβ: Amyloid-β; Ctx: cortex; DAPI: 4,6-diamino-2-phenylindole; GGT5: gamma-glutamyltransferase 5; Hip: hippocampus; WT: wide type.

Article Snippet: The secondary antibodies included goat anti-mouse IgG-Cy3 (1:100, Boster, Cat# BA1031, RRID: AB_10890402), goat anti-rabbit IgG-Cy3 (1:300, Boster, Cat# BA1032, RRID: AB_2716305), goat anti-rabbit IgG-Cy3 (1:500, Invitrogen, Shanghai, China, A10520, RRID: AB_10563288), and goat anti-mouse IgG-488 (1:300, Invitrogen, Cat# A32723, RRID: AB_2633275).

Techniques: Over Expression, Immunofluorescence, Staining, Plasmid Preparation