cut pselect gfpzeo  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cut pselect gfpzeo
    Cut Pselect Gfpzeo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cut pselect gfpzeo/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cut pselect gfpzeo - by Bioz Stars, 2020-05
    93/100 stars

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    Transformation Assay:

    Article Title: Longitudinal within-host evolution of HIV Nef-mediated CD4, HLA and SERINC5 downregulation activity: a case study
    Article Snippet: .. As described in [ ], nef amplicons were digested with AscI and SacII, ligated into cut pSELECT-GFPzeo (T4 ligase; Thermo Fisher® ), and transformed into chemically competent E. coli (E. cloni 10G DUOs; Lucigen). .. Plasmid DNA from a minimum of three colonies per transformation was isolated, purified (Thermo Fisher® OMEGA EZNA plasmid minikit) and re-sequenced to confirm identity.

    Article Title: HIV Subtype and Nef-Mediated Immune Evasion Function Correlate with Viral Reservoir Size in Early-Treated Individuals
    Article Snippet: .. Participant-derived nef amplicons were digested with AscI and SacII, ligated into cut pSELECT-GFPzeo (T4 ligase; Thermo Fisher), transformed into chemically competent Escherichia coli (E. cloni 10G DUOs; Lucigen), plated onto zeocin-containing Luria-Bertani (LB) agar plates, and incubated for 16 to 18 h. A minimum of three colonies per participant were subsequently propagated overnight in zeocin-containing LB broth, after which plasmid DNA was purified (Omega EZNA plasmid minikit; Thermo Fisher). .. The presence of an insert was verified by restriction enzyme digestion, followed by agarose gel electrophoresis.

    Incubation:

    Article Title: HIV Subtype and Nef-Mediated Immune Evasion Function Correlate with Viral Reservoir Size in Early-Treated Individuals
    Article Snippet: .. Participant-derived nef amplicons were digested with AscI and SacII, ligated into cut pSELECT-GFPzeo (T4 ligase; Thermo Fisher), transformed into chemically competent Escherichia coli (E. cloni 10G DUOs; Lucigen), plated onto zeocin-containing Luria-Bertani (LB) agar plates, and incubated for 16 to 18 h. A minimum of three colonies per participant were subsequently propagated overnight in zeocin-containing LB broth, after which plasmid DNA was purified (Omega EZNA plasmid minikit; Thermo Fisher). .. The presence of an insert was verified by restriction enzyme digestion, followed by agarose gel electrophoresis.

    Plasmid Preparation:

    Article Title: HIV Subtype and Nef-Mediated Immune Evasion Function Correlate with Viral Reservoir Size in Early-Treated Individuals
    Article Snippet: .. Participant-derived nef amplicons were digested with AscI and SacII, ligated into cut pSELECT-GFPzeo (T4 ligase; Thermo Fisher), transformed into chemically competent Escherichia coli (E. cloni 10G DUOs; Lucigen), plated onto zeocin-containing Luria-Bertani (LB) agar plates, and incubated for 16 to 18 h. A minimum of three colonies per participant were subsequently propagated overnight in zeocin-containing LB broth, after which plasmid DNA was purified (Omega EZNA plasmid minikit; Thermo Fisher). .. The presence of an insert was verified by restriction enzyme digestion, followed by agarose gel electrophoresis.

    Purification:

    Article Title: HIV Subtype and Nef-Mediated Immune Evasion Function Correlate with Viral Reservoir Size in Early-Treated Individuals
    Article Snippet: .. Participant-derived nef amplicons were digested with AscI and SacII, ligated into cut pSELECT-GFPzeo (T4 ligase; Thermo Fisher), transformed into chemically competent Escherichia coli (E. cloni 10G DUOs; Lucigen), plated onto zeocin-containing Luria-Bertani (LB) agar plates, and incubated for 16 to 18 h. A minimum of three colonies per participant were subsequently propagated overnight in zeocin-containing LB broth, after which plasmid DNA was purified (Omega EZNA plasmid minikit; Thermo Fisher). .. The presence of an insert was verified by restriction enzyme digestion, followed by agarose gel electrophoresis.

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  • 93
    Thermo Fisher cut pselect gfpzeo
    Cut Pselect Gfpzeo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cut pselect gfpzeo/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cut pselect gfpzeo - by Bioz Stars, 2020-05
    93/100 stars
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