Journal: Frontiers in Microbiology

Article Title: Evaluation of phages and liposomes as combination therapy to counteract Pseudomonas aeruginosa infection in wild-type and CFTR -null models

doi: 10.3389/fmicb.2022.979610

Figure Lengend Snippet: PS/PA liposomes and CKΦ administration does not elicit toxic effects on human CF cells. (A) Cell viability, evaluated by MTT assay, in immortalized human bronchial epithelial cells CuFi-1 expressing F508del CFTR control, treated with phosphatidylserine/phosphatidic acid (PS/PA) liposome, CKΦ or combination of PS/PA liposome-CKΦ for 48 h. (B) Cell viability, evaluated by MTT assay, in THP-1 activated macrophage-like cells treated or not with the CFTR inhibitor. Effects of single or combined PS/PA liposome and CKΦ-administration were evaluated after 48 h of treatment. Unpaired Student’s t test: * p < 0.05; ns, not significant. Results are presented as mean ± SEM.

Article Snippet: The human CF bronchial epithelial cell line CuFi-1 (ATCC CRL-4013™) were grown as monolayer cultures as previously described ( ).

Techniques: MTT Assay, Expressing

Journal: PLoS ONE

Article Title: Immunomodulatory function of the cystic fibrosis modifier gene BPIFA1

doi: 10.1371/journal.pone.0227067

Figure Lengend Snippet: Quantification of IL-8 and IL-6 by ELISA produced by IB3-1, CuFi-1 and CFBE41o- airway epithelial cell lines that were unstimulated, stimulated with heat-killed P . aeruginosa , pretreated with recombinant BPIFA1 for 30 min followed by stimulation with heat-killed P . aeruginosa , or pretreated with recombinant BPIFB1 for 30 min followed by stimulation with heat-killed P . aeruginosa . ANOVA was used to identify statistical differences in IL-6 and IL-8 levels between conditions with * denoting P < 0.05.

Article Snippet: IB3-1 and CuFi-1 CF airway epithelial cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Produced, Recombinant