cu na edta  (Millipore)

 
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  • 93
    Name:
    Ethylenediaminetetraacetic acid copper II disodium salt
    Description:

    Catalog Number:
    03668
    Price:
    None
    Applications:
    Used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals
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    Structured Review

    Millipore cu na edta
    Ethylenediaminetetraacetic acid copper II disodium salt

    https://www.bioz.com/result/cu na edta/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cu na edta - by Bioz Stars, 2021-07
    93/100 stars

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    other:

    Article Title: The Effects of Salicylic Acid and Its Derivatives on Increasing Pomegranate Fruit Quality and Bioactive Compounds at Harvest and During Storage
    Article Snippet: Briefly, 5 g of frozen arils were homogenized with 5 mL of methanol: water (5:95) containing 0.1 mM citric acid, 0.05 mM ethylenediamine tetraacetic acid disodium salt, and 4 mM NaF for 30 s on an Ultraturrax, T18 basic, IKA, Berlin, Germany.

    Article Title: Calcium-dependent lateral organization in phosphatidylinositol (4,5) bisphosphate (PIP2)- and cholesterol-containing monolayers
    Article Snippet: Subphase reagents (HEPES, EDTA, CaCl2, and NaCl) were purchased from Sigma (St. Louis, MO).

    Incubation:

    Article Title: TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases
    Article Snippet: Intestinal macrophages were isolated from human intestinal tissue samples and biopsies as previously described ( , ). .. Briefly, small pieces of intestinal mucosa were incubated with 5 mM EDTA/2 mM DTT, followed by digestion with 200 U/ml collagenase (Type IV; Sigma-Aldrich) plus 50 U/ml DNAse (Type I, grade II; Roche). .. Macrophages were enriched in a Percoll density gradient and subsequently purified using a depleting MACS procedure.

    Lysis:

    Article Title: Astroglial connexin43 contributes to neuronal suffering in a mouse model of Alzheimer's disease
    Article Snippet: Brain hemisphere slices from 9-month-old APP/PS1 mice (300 μ m, two slices for each condition) were treated or not with minocycline (50 nM, Sigma-Aldrich) during 2 h, transferred into tubes, frozen and stored at −80 °C until processing. .. Slices were immersed in ice-cold lysis buffer containing (in mM) Tris-HCl 50, NaCl 100, EDTA 2, 1% Triton-X100 and 1% protease inhibitor cocktail (Sigma-Aldrich), and sonicated for 10 s. Samples were centrifuged at 13 200 r.p.m. for 20 min at 4 °C and supernatants stored at −80 °C. .. A BCA kit (Thermo Scientific, France) was used for protein determination.

    Article Title: Anti-Aging Effect of Nigella Sativa Fixed Oil on D-Galactose-Induced Aging in Mice
    Article Snippet: The levels of alanine amino transferase (ALT) and aminotransferase (AST) were determined using a Mindary auto analyzer (BS 800) according to the kit’s protocols. .. At the first step in the Western blot analysis, brain and liver tissue samples were lysed in lysis buffer containing 50-mM Tris-HCl (pH: 7.4), 2-mM EDTA, 2-mM EGTA, 10-mM NaF, 1-mM sodium orthovanadate (Na3VO4), 10-mM β -glycerophosphate, 0.2% W/V sodium deoxycholate, 1-mM phenylmethylsulfonyl fluoride (PMSF), and a complete protease inhibitor cocktail (Sigma P8340). .. Then, the total proteins were separated using 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred to polyvinylidene fluoride (PVDF) membranes.

    Protease Inhibitor:

    Article Title: Astroglial connexin43 contributes to neuronal suffering in a mouse model of Alzheimer's disease
    Article Snippet: Brain hemisphere slices from 9-month-old APP/PS1 mice (300 μ m, two slices for each condition) were treated or not with minocycline (50 nM, Sigma-Aldrich) during 2 h, transferred into tubes, frozen and stored at −80 °C until processing. .. Slices were immersed in ice-cold lysis buffer containing (in mM) Tris-HCl 50, NaCl 100, EDTA 2, 1% Triton-X100 and 1% protease inhibitor cocktail (Sigma-Aldrich), and sonicated for 10 s. Samples were centrifuged at 13 200 r.p.m. for 20 min at 4 °C and supernatants stored at −80 °C. .. A BCA kit (Thermo Scientific, France) was used for protein determination.

    Article Title: Cardiac Myosin Light Chain Kinase Is Necessary for Myosin Regulatory Light Chain Phosphorylation and Cardiac Performance in Vivo *
    Article Snippet: Additional Western blotting was performed by SDS-PAGE on other proteins solubilized in the 8 m urea buffer. .. For preparation of soluble proteins, tissues were homogenized in buffer at pH 7.6 containing (in m m ) Tris-HCl 50, EGTA 2, EDTA 2, NaCl 150, dithiothreitol, 1% Nonidet P-40, and 10 μl/ml protease inhibitor mixture (Sigma). ..

    Article Title: Anti-Aging Effect of Nigella Sativa Fixed Oil on D-Galactose-Induced Aging in Mice
    Article Snippet: The levels of alanine amino transferase (ALT) and aminotransferase (AST) were determined using a Mindary auto analyzer (BS 800) according to the kit’s protocols. .. At the first step in the Western blot analysis, brain and liver tissue samples were lysed in lysis buffer containing 50-mM Tris-HCl (pH: 7.4), 2-mM EDTA, 2-mM EGTA, 10-mM NaF, 1-mM sodium orthovanadate (Na3VO4), 10-mM β -glycerophosphate, 0.2% W/V sodium deoxycholate, 1-mM phenylmethylsulfonyl fluoride (PMSF), and a complete protease inhibitor cocktail (Sigma P8340). .. Then, the total proteins were separated using 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred to polyvinylidene fluoride (PVDF) membranes.

    Sonication:

    Article Title: Astroglial connexin43 contributes to neuronal suffering in a mouse model of Alzheimer's disease
    Article Snippet: Brain hemisphere slices from 9-month-old APP/PS1 mice (300 μ m, two slices for each condition) were treated or not with minocycline (50 nM, Sigma-Aldrich) during 2 h, transferred into tubes, frozen and stored at −80 °C until processing. .. Slices were immersed in ice-cold lysis buffer containing (in mM) Tris-HCl 50, NaCl 100, EDTA 2, 1% Triton-X100 and 1% protease inhibitor cocktail (Sigma-Aldrich), and sonicated for 10 s. Samples were centrifuged at 13 200 r.p.m. for 20 min at 4 °C and supernatants stored at −80 °C. .. A BCA kit (Thermo Scientific, France) was used for protein determination.

    Western Blot:

    Article Title: Anti-Aging Effect of Nigella Sativa Fixed Oil on D-Galactose-Induced Aging in Mice
    Article Snippet: The levels of alanine amino transferase (ALT) and aminotransferase (AST) were determined using a Mindary auto analyzer (BS 800) according to the kit’s protocols. .. At the first step in the Western blot analysis, brain and liver tissue samples were lysed in lysis buffer containing 50-mM Tris-HCl (pH: 7.4), 2-mM EDTA, 2-mM EGTA, 10-mM NaF, 1-mM sodium orthovanadate (Na3VO4), 10-mM β -glycerophosphate, 0.2% W/V sodium deoxycholate, 1-mM phenylmethylsulfonyl fluoride (PMSF), and a complete protease inhibitor cocktail (Sigma P8340). .. Then, the total proteins were separated using 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred to polyvinylidene fluoride (PVDF) membranes.

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  • 93
    Millipore cu na edta
    Cu Na Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cu na edta/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cu na edta - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

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