jena bioscience jbscreen classic  (Jena Bioscience)


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  • 93
    Name:
    JBScreen Classic 1
    Description:

    Catalog Number:
    CS-101L
    Price:
    149.0
    Category:
    Crystallography
    Size:
    24 solutions
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    Structured Review

    Jena Bioscience jena bioscience jbscreen classic
    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 <t>(Jena</t> Bioscience <t>JBScreen</t> Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    https://www.bioz.com/result/jena bioscience jbscreen classic/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jena bioscience jbscreen classic - by Bioz Stars, 2021-05
    93/100 stars

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    1) Product Images from "Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state"

    Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2018.09.005

    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.
    Figure Legend Snippet: LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    Techniques Used:

    Related Articles

    Crystallization Assay:

    Article Title: Crystal structure of an iron superoxide dismutase from the pathogenic amoeba Acanthamoeba castellanii.
    Article Snippet: .. The iron superoxide dismutase found in the pathogenic amoeba Acanthamoeba castellanii (AcFeSOD) may play essential roles in the survival of the parasite, not only by protecting it from endogenous oxidative stress but also by detoxifying oxidative killing of the parasite by host immune effector cells. ..

    Article Title: Structural analysis of the reducing-end xylose-releasing exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 deciphers its molecular specificity.
    Article Snippet: The purity of the protein was verified by SDS/PAGE, and the concentration of the enzymes was measured with NanoDrop ND1000 (NanoDrop Technologies, Inc, Wilmington, DE, USA), using an extinction coefficient (e) at 280 nm of 99 950 for all the variants. .. Crystallization and data collection Initial crystallization conditions for Rex8A (5 mg mL 1 in 20 mM Tris/HCl pH 7) were explored by high-throughput techniques with a NanoDrop robot (Innovadyne Technologies Inc., Wilmington, DE, USA), using six different commercially screens: PACT and JCSG + Suites from Qiagen (QIAGEN N.V., Venlo, The Netherlands); JBScreen Classic 1–4 from Jena Bioscience (Jena, Germany); and Index, Crystal Screen, and SaltRx packages from Hampton Research (Aliso Viejo, CA, USA). .. These assays were carried out using the sitting-drop vapor-diffusion method in MRC 96 well crystallization plates (Molecular Dimensions, Sheffield, UK).

    Article Title: Crystallographic and kinetic study of riboflavin synthase from Brucella abortus, a chemotherapeutic target with an enhanced intrinsic flexibility
    Article Snippet: The quality of the final preparation was checked by SDS–PAGE (15% gel) and UV spectrophotometry. .. Initial crystallization trials were performed in 96-well plates using a Honeybee 963 robot (Digilab, Marlborough, Massachusetts, USA) and JBScreen Classic (Jena Bioscience, Germany) in a sitting-drop vapour-diffusion configuration. .. We were able to reproduce and optimize crystal growth for several of these conditions, and the best diffracting RS crystals (RS-APO) were eventually obtained by the hanging-drop method by mixing 2 µl concentrated protein stock with an equal amount of a crystallization solution consisting of 12%( w / v ) PEG 8000, 10%( w / v ) glycerol, 0.5 M potassium chloride.

    Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state
    Article Snippet: .. Initial crystallization trials were performed at 5.3 mg ml−1 in 96-well sitting-drop vapour-diffusion Greiner 609120 plates (Monroe, North Carolina, USA) using a Honeybee 963 robot (Digilab, Marlborough, Massachusetts, USA) and the following crystallization kits: Jena Bioscience JBScreen Classic and Pentaerythritol (Jena, Germany), and Hampton Research Crystal Screen, Crystal Screen 2, PEG/Ion, PEG/Ion 2, PEGRx 1 and PEGRx 2 (Aliso Viejo, California, USA). ..

    Article Title: The structure of human leucine carboxyl methyltransferase 1 that regulates protein phosphatase PP2A.
    Article Snippet: Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A). .. Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A). .. Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A).

    Article Title: Structure and reaction mechanism of L-rhamnulose kinase from Escherichia coli.
    Article Snippet: .. Bacterial L-rhamnulose kinase participates in the degradation of L-rhamnose, which is ubiquitous and particularly abundant in some plants. ..

    Article Title: Modular Architecture and Unique Teichoic Acid Recognition Features of Choline-Binding Protein L (CbpL) Contributing to Pneumococcal Pathogenesis
    Article Snippet: Crystallization of CbpLCBM Prior to setting up crystallization assays, CbpL buffer was exchanged to 10 mM Tris-HCl pH 7.5 and 140 mM choline chloride by dialysis. .. First crystallization screenings were performed by high-throughput techniques in a NanoDrop robot and Innovadyne SD-2 microplates (Innovadyne Technologies Inc.), screening PACT Suite and JCSG Suite (Qiagen), JBScreen Classic 1–4 and 6 (Jena Bioscience) and Crystal Screen, Crystal Screen 2 and Index HT (Hampton Research). .. Positive conditions were optimized by hanging-drop vapour diffusion method by mixing 1 μL of protein solution and 1 μL of precipitant solution, equilibrated against 500 μL of precipitant solution.

    High Throughput Screening Assay:

    Article Title: Structural analysis of the reducing-end xylose-releasing exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 deciphers its molecular specificity.
    Article Snippet: The purity of the protein was verified by SDS/PAGE, and the concentration of the enzymes was measured with NanoDrop ND1000 (NanoDrop Technologies, Inc, Wilmington, DE, USA), using an extinction coefficient (e) at 280 nm of 99 950 for all the variants. .. Crystallization and data collection Initial crystallization conditions for Rex8A (5 mg mL 1 in 20 mM Tris/HCl pH 7) were explored by high-throughput techniques with a NanoDrop robot (Innovadyne Technologies Inc., Wilmington, DE, USA), using six different commercially screens: PACT and JCSG + Suites from Qiagen (QIAGEN N.V., Venlo, The Netherlands); JBScreen Classic 1–4 from Jena Bioscience (Jena, Germany); and Index, Crystal Screen, and SaltRx packages from Hampton Research (Aliso Viejo, CA, USA). .. These assays were carried out using the sitting-drop vapor-diffusion method in MRC 96 well crystallization plates (Molecular Dimensions, Sheffield, UK).

    Article Title: The structure of human leucine carboxyl methyltransferase 1 that regulates protein phosphatase PP2A.
    Article Snippet: Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A). .. Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A). .. Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A).

    Article Title: Modular Architecture and Unique Teichoic Acid Recognition Features of Choline-Binding Protein L (CbpL) Contributing to Pneumococcal Pathogenesis
    Article Snippet: Crystallization of CbpLCBM Prior to setting up crystallization assays, CbpL buffer was exchanged to 10 mM Tris-HCl pH 7.5 and 140 mM choline chloride by dialysis. .. First crystallization screenings were performed by high-throughput techniques in a NanoDrop robot and Innovadyne SD-2 microplates (Innovadyne Technologies Inc.), screening PACT Suite and JCSG Suite (Qiagen), JBScreen Classic 1–4 and 6 (Jena Bioscience) and Crystal Screen, Crystal Screen 2 and Index HT (Hampton Research). .. Positive conditions were optimized by hanging-drop vapour diffusion method by mixing 1 μL of protein solution and 1 μL of precipitant solution, equilibrated against 500 μL of precipitant solution.

    Purification:

    Article Title: The structure of human leucine carboxyl methyltransferase 1 that regulates protein phosphatase PP2A.
    Article Snippet: Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A). .. Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A). .. Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A).

    Variant Assay:

    Article Title: The structure of human leucine carboxyl methyltransferase 1 that regulates protein phosphatase PP2A.
    Article Snippet: Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A). .. Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A). .. Leucine carboxyl methyltransferase 1 (LCMT1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2A (PP2A).

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  • 93
    Jena Bioscience jena bioscience jbscreen classic
    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 <t>(Jena</t> Bioscience <t>JBScreen</t> Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.
    Jena Bioscience Jbscreen Classic, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jena bioscience jbscreen classic/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jena bioscience jbscreen classic - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state

    doi: 10.1016/j.bbrep.2018.09.005

    Figure Lengend Snippet: LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    Article Snippet: Initial crystallization trials were performed at 5.3 mg ml−1 in 96-well sitting-drop vapour-diffusion Greiner 609120 plates (Monroe, North Carolina, USA) using a Honeybee 963 robot (Digilab, Marlborough, Massachusetts, USA) and the following crystallization kits: Jena Bioscience JBScreen Classic and Pentaerythritol (Jena, Germany), and Hampton Research Crystal Screen, Crystal Screen 2, PEG/Ion, PEG/Ion 2, PEGRx 1 and PEGRx 2 (Aliso Viejo, California, USA).

    Techniques: