microbeads  (Millipore)


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    Name:
    Latex beads
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    Catalog Number:
    l2278
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    Structured Review

    Millipore microbeads
    XRD. Spectra of HEPES powder used to buffer the crosslinking solution, alginate powder used to make the <t>microbeads,</t> buffered microbeads after 5 weeks in vivo , NaCl from the database, non-buffered microbeads after 5 weeks in vivo , and hydroxyapatite from the database.

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    1) Product Images from "Regulating in vivo calcification of alginate microbeads"

    Article Title: Regulating in vivo calcification of alginate microbeads

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2010.03.001

    XRD. Spectra of HEPES powder used to buffer the crosslinking solution, alginate powder used to make the microbeads, buffered microbeads after 5 weeks in vivo , NaCl from the database, non-buffered microbeads after 5 weeks in vivo , and hydroxyapatite from the database.
    Figure Legend Snippet: XRD. Spectra of HEPES powder used to buffer the crosslinking solution, alginate powder used to make the microbeads, buffered microbeads after 5 weeks in vivo , NaCl from the database, non-buffered microbeads after 5 weeks in vivo , and hydroxyapatite from the database.

    Techniques Used: In Vivo

    Gross-visualization of alginate microbead mineralization. (A) Microbeads before implantation or injection under a light microscope (Bar = 100 μm). (B) Live/dead staining of human ASCs within a single microbead post-injection (Bar = 25 μm) (C) Visualization of mineralized microbeads 3 months post-implantation under a light microscope (Bar = 100 μm). (D) Mineralized microbeads 3 months post-implantation. (E) Mineralized microbeads 1 month post-injection.
    Figure Legend Snippet: Gross-visualization of alginate microbead mineralization. (A) Microbeads before implantation or injection under a light microscope (Bar = 100 μm). (B) Live/dead staining of human ASCs within a single microbead post-injection (Bar = 25 μm) (C) Visualization of mineralized microbeads 3 months post-implantation under a light microscope (Bar = 100 μm). (D) Mineralized microbeads 3 months post-implantation. (E) Mineralized microbeads 1 month post-injection.

    Techniques Used: Injection, Light Microscopy, Staining

    Histology of in vivo microbeads. von Kossa with nuclear fast red counter stain to determine calcification for representative (A) non-buffered, (B) barium chloride, (C), bisphosphonate, (D) buffered samples in vivo after 2 months. Bar represents 100 μm for all images.
    Figure Legend Snippet: Histology of in vivo microbeads. von Kossa with nuclear fast red counter stain to determine calcification for representative (A) non-buffered, (B) barium chloride, (C), bisphosphonate, (D) buffered samples in vivo after 2 months. Bar represents 100 μm for all images.

    Techniques Used: In Vivo, Staining

    MicroCT analysis of non-buffered in vivo samples. (A) Representative X-ray cross-section of subcutaneously implanted non-buffered microbeads after 5 weeks in vivo . (B) 3-D reconstruction of subcutaneously implanted non-buffered microbeads after 5 weeks in vivo . (C) Representative sagittal X-ray cross-section of intramuscularly implanted non-buffered microbeads near the tibia after 5 weeks in vivo . (D) 3-D reconstruction of intramuscular implanted non-buffered microbeads along with the tibia after 5 weeks in vivo . Bar represents 1 mm for all images.
    Figure Legend Snippet: MicroCT analysis of non-buffered in vivo samples. (A) Representative X-ray cross-section of subcutaneously implanted non-buffered microbeads after 5 weeks in vivo . (B) 3-D reconstruction of subcutaneously implanted non-buffered microbeads after 5 weeks in vivo . (C) Representative sagittal X-ray cross-section of intramuscularly implanted non-buffered microbeads near the tibia after 5 weeks in vivo . (D) 3-D reconstruction of intramuscular implanted non-buffered microbeads along with the tibia after 5 weeks in vivo . Bar represents 1 mm for all images.

    Techniques Used: In Vivo

    FTIR. Spectra of (A) non-buffered microbeads after 5 weeks in vivo , (B) buffered microbeads after 5 weeks in vivo , (C) HEPES powder used to buffer the crosslinking solution, and (D) alginate powder used to make the microbeads.
    Figure Legend Snippet: FTIR. Spectra of (A) non-buffered microbeads after 5 weeks in vivo , (B) buffered microbeads after 5 weeks in vivo , (C) HEPES powder used to buffer the crosslinking solution, and (D) alginate powder used to make the microbeads.

    Techniques Used: In Vivo

    2) Product Images from "Regulating in vivo calcification of alginate microbeads"

    Article Title: Regulating in vivo calcification of alginate microbeads

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2010.03.001

    Histology of  in vivo  microbeads. von Kossa with nuclear fast red counter stain to determine calcification for representative (A) non-buffered, (B) barium chloride, (C), bisphosphonate, (D) buffered samples  in vivo  after 2 months. Bar represents 100 μm for all images.
    Figure Legend Snippet: Histology of in vivo microbeads. von Kossa with nuclear fast red counter stain to determine calcification for representative (A) non-buffered, (B) barium chloride, (C), bisphosphonate, (D) buffered samples in vivo after 2 months. Bar represents 100 μm for all images.

    Techniques Used: In Vivo, Staining

    3) Product Images from "Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers"

    Article Title: Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2020.120109

    Chemical characterisation of treated collagen films: The ninhydrin assay was used to quantify the degree of collagen film crosslinking using EDC-NHS, genipin and TG2. The percentages on the x-axis represent the concentrations of crosslinking solutions, with the resulting degree of crosslinking (y-axis) depending on the nature of the crosslinking reaction applied. Samples exhibiting a significant difference from the non-crosslinked sample are noted with a *.
    Figure Legend Snippet: Chemical characterisation of treated collagen films: The ninhydrin assay was used to quantify the degree of collagen film crosslinking using EDC-NHS, genipin and TG2. The percentages on the x-axis represent the concentrations of crosslinking solutions, with the resulting degree of crosslinking (y-axis) depending on the nature of the crosslinking reaction applied. Samples exhibiting a significant difference from the non-crosslinked sample are noted with a *.

    Techniques Used:

    Mechanical characterisation of collagen films crosslinked with EDC-NHS, genipin and TG2: a) Tensile modulus b) Strain at failure and c) Tensile failure. Subfigures (i) represent the data with respect to the concentrations of various crosslinkers. Samples exhibiting a significant difference from the non-crosslinked sample are noted with a *, and from the Tris buffered sample are marked with a ^. Values outside brackets represent crosslinker concentration whereas values in brackets represent the ‘degree of crosslinking’ as determined by the ninhydrin free amine assay for a given condition. Subfigures (ii) summarise the data as a function of the ’degree of crosslinking’.
    Figure Legend Snippet: Mechanical characterisation of collagen films crosslinked with EDC-NHS, genipin and TG2: a) Tensile modulus b) Strain at failure and c) Tensile failure. Subfigures (i) represent the data with respect to the concentrations of various crosslinkers. Samples exhibiting a significant difference from the non-crosslinked sample are noted with a *, and from the Tris buffered sample are marked with a ^. Values outside brackets represent crosslinker concentration whereas values in brackets represent the ‘degree of crosslinking’ as determined by the ninhydrin free amine assay for a given condition. Subfigures (ii) summarise the data as a function of the ’degree of crosslinking’.

    Techniques Used: Concentration Assay

    Integrin mediated adhesion of human dermal fibroblasts and cellular proliferation over 7 days in culture. (a) LDH attachment assay performed on non-crosslinked and EDC-NHS, genipin and TG2 crosslinked films. Integrin-specific and non-specific binding were separated through the addition of EDTA or MgCl 2 to cell media. (b) Cell proliferation over 7 days was evaluated using the MTS assay. A * indicates p
    Figure Legend Snippet: Integrin mediated adhesion of human dermal fibroblasts and cellular proliferation over 7 days in culture. (a) LDH attachment assay performed on non-crosslinked and EDC-NHS, genipin and TG2 crosslinked films. Integrin-specific and non-specific binding were separated through the addition of EDTA or MgCl 2 to cell media. (b) Cell proliferation over 7 days was evaluated using the MTS assay. A * indicates p

    Techniques Used: Binding Assay, MTS Assay

    4) Product Images from "Detection of transglutaminase activity using click chemistry"

    Article Title: Detection of transglutaminase activity using click chemistry

    Journal: Amino Acids

    doi: 10.1007/s00726-011-1198-2

    TG2-dependency of substrate crosslinking. HeLa cells were transfected with siRNAs directed against TG2 (TG2 siRNA 1 or 2) or GFP (negative control) and, after 1 day, incubated with substrate 2 . In addition, untransfected cells were incubated without (−) or with TG2 inhibitor Z006 during the incubation with substrate 2 . The proteins crosslinked with the substrate were labeled with biotin-alkyne and analyzed by western blotting using labeled streptavidin to detect biotinylated proteins ( upper panel ) or antibodies directed against TG2 ( middle panel ) or γ-tubulin ( lower panel ). The size and position of the protein markers are indicated on the left
    Figure Legend Snippet: TG2-dependency of substrate crosslinking. HeLa cells were transfected with siRNAs directed against TG2 (TG2 siRNA 1 or 2) or GFP (negative control) and, after 1 day, incubated with substrate 2 . In addition, untransfected cells were incubated without (−) or with TG2 inhibitor Z006 during the incubation with substrate 2 . The proteins crosslinked with the substrate were labeled with biotin-alkyne and analyzed by western blotting using labeled streptavidin to detect biotinylated proteins ( upper panel ) or antibodies directed against TG2 ( middle panel ) or γ-tubulin ( lower panel ). The size and position of the protein markers are indicated on the left

    Techniques Used: Transfection, Negative Control, Incubation, Labeling, Western Blot

    Analysis of TG2-catalyzed crosslinking of azide-functionalized amine-donor substrates. Substrate 1 , 2 , 3 and BPA (1 mM) were incubated with the amine-acceptor peptide biotin-GQEPVR (1 mM) after which the amount of γ-glutamyl-monoamine crosslink product was monitored by HPLC and plotted versus time
    Figure Legend Snippet: Analysis of TG2-catalyzed crosslinking of azide-functionalized amine-donor substrates. Substrate 1 , 2 , 3 and BPA (1 mM) were incubated with the amine-acceptor peptide biotin-GQEPVR (1 mM) after which the amount of γ-glutamyl-monoamine crosslink product was monitored by HPLC and plotted versus time

    Techniques Used: Incubation, High Performance Liquid Chromatography

    Detection of TG2-catalyzed crosslinking of HspB6 via CuAAC and SPAAC. a Substrates 1 , 2 and 3 were crosslinked by TG2 onto HspB6 and subsequently biotinylated via CuAAC using biotin-alkyne. b Substrate 2 was crosslinked by TG2 onto HspB6 and subsequently biotinylated via CuAAC, using biotin-alkyne, and SPAAC, using DIBO, DIBAC and BCN. As negative controls, TG2, the azide-containing substrate and/or biotin-alkyne were omitted. The biotinylated TG2 substrate BPA was used as a positive control for TG2 activity. HspB6 isoforms were separated by SDS-PAGE and analyzed by western blotting using either labeled streptavidin to detect biotinylated proteins ( upper panels ) or antibodies directed to HspB6 ( lower panels ). The positions of HspB6 monomers, dimers and multimers are indicated on the right . Intramolecular crosslinked HspB6 monomers are indicated with i . The size and position of the protein markers are indicated on the left
    Figure Legend Snippet: Detection of TG2-catalyzed crosslinking of HspB6 via CuAAC and SPAAC. a Substrates 1 , 2 and 3 were crosslinked by TG2 onto HspB6 and subsequently biotinylated via CuAAC using biotin-alkyne. b Substrate 2 was crosslinked by TG2 onto HspB6 and subsequently biotinylated via CuAAC, using biotin-alkyne, and SPAAC, using DIBO, DIBAC and BCN. As negative controls, TG2, the azide-containing substrate and/or biotin-alkyne were omitted. The biotinylated TG2 substrate BPA was used as a positive control for TG2 activity. HspB6 isoforms were separated by SDS-PAGE and analyzed by western blotting using either labeled streptavidin to detect biotinylated proteins ( upper panels ) or antibodies directed to HspB6 ( lower panels ). The positions of HspB6 monomers, dimers and multimers are indicated on the right . Intramolecular crosslinked HspB6 monomers are indicated with i . The size and position of the protein markers are indicated on the left

    Techniques Used: Positive Control, Activity Assay, SDS Page, Western Blot, Labeling

    5) Product Images from "Adipose-derived Stem/Stromal Cells on Electrospun Fibrin Microfiber Bundles Enable Moderate Muscle Reconstruction in a Volumetric Muscle Loss Model"

    Article Title: Adipose-derived Stem/Stromal Cells on Electrospun Fibrin Microfiber Bundles Enable Moderate Muscle Reconstruction in a Volumetric Muscle Loss Model

    Journal: Cell Transplantation

    doi: 10.1177/0963689718805370

    ASC growth and myogenesis on electrospun fibrin fibers. (A) Live (green)/Dead (red) staining of cells seeded at 40 000 cells/μl at days 0 and 21. (B) Histological cross-section stained with DAPI (blue) showing ASC infiltration to the fiber interior at day 21. (C) Quantification of total DNA content of fibers seeded with ASCs at the three seeding densities at days 0, 7, 14, and 21. *p
    Figure Legend Snippet: ASC growth and myogenesis on electrospun fibrin fibers. (A) Live (green)/Dead (red) staining of cells seeded at 40 000 cells/μl at days 0 and 21. (B) Histological cross-section stained with DAPI (blue) showing ASC infiltration to the fiber interior at day 21. (C) Quantification of total DNA content of fibers seeded with ASCs at the three seeding densities at days 0, 7, 14, and 21. *p

    Techniques Used: Staining

    6) Product Images from "Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers"

    Article Title: Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2020.120109

    Mechanical characterisation of collagen films crosslinked with EDC-NHS, genipin and TG2: a) Tensile modulus b) Strain at failure and c) Tensile failure. Subfigures (i) represent the data with respect to the concentrations of various crosslinkers. Samples exhibiting a significant difference from the non-crosslinked sample are noted with a *, and from the Tris buffered sample are marked with a ^. Values outside brackets represent crosslinker concentration whereas values in brackets represent the ‘degree of crosslinking’ as determined by the ninhydrin free amine assay for a given condition. Subfigures (ii) summarise the data as a function of the ’degree of crosslinking’.
    Figure Legend Snippet: Mechanical characterisation of collagen films crosslinked with EDC-NHS, genipin and TG2: a) Tensile modulus b) Strain at failure and c) Tensile failure. Subfigures (i) represent the data with respect to the concentrations of various crosslinkers. Samples exhibiting a significant difference from the non-crosslinked sample are noted with a *, and from the Tris buffered sample are marked with a ^. Values outside brackets represent crosslinker concentration whereas values in brackets represent the ‘degree of crosslinking’ as determined by the ninhydrin free amine assay for a given condition. Subfigures (ii) summarise the data as a function of the ’degree of crosslinking’.

    Techniques Used: Concentration Assay

    7) Product Images from "Zeptomole Detection of C-Reactive Protein in Serum by a Nanoparticle Amplified Surface Plasmon Resonance Imaging Aptasensor"

    Article Title: Zeptomole Detection of C-Reactive Protein in Serum by a Nanoparticle Amplified Surface Plasmon Resonance Imaging Aptasensor

    Journal: Scientific Reports

    doi: 10.1038/srep05129

    Detection of CRP using a sandwich assay spiked in human serum. (a) Binding of NanoEnhancers (CRP_specific_Aptamer-QDs) after the injection of PEG-SH and CRP (500 pg/ml) spiked in human serum to Cys/Glu/extravidin/Aptamer surface coated gold chip and (b) SPRi difference images showing the binding of NanoEnhancers to CRP (left) and control (right). (c) A plot profile of the SPRi difference image revealing intensity values to the area indicated by the yellow line in figure 4b and shows the change in contrast due to the binding of NanoEnhancers in spots functionalized with CRP_Specific aptamer (left, A1–A5), control aptamer (right, C1–C5). The middle region of the line is the background.
    Figure Legend Snippet: Detection of CRP using a sandwich assay spiked in human serum. (a) Binding of NanoEnhancers (CRP_specific_Aptamer-QDs) after the injection of PEG-SH and CRP (500 pg/ml) spiked in human serum to Cys/Glu/extravidin/Aptamer surface coated gold chip and (b) SPRi difference images showing the binding of NanoEnhancers to CRP (left) and control (right). (c) A plot profile of the SPRi difference image revealing intensity values to the area indicated by the yellow line in figure 4b and shows the change in contrast due to the binding of NanoEnhancers in spots functionalized with CRP_Specific aptamer (left, A1–A5), control aptamer (right, C1–C5). The middle region of the line is the background.

    Techniques Used: Binding Assay, Injection, Chromatin Immunoprecipitation

    8) Product Images from "Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers"

    Article Title: Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2020.120109

    Mechanical characterisation of collagen films crosslinked with EDC-NHS, genipin and TG2: a) Tensile modulus b) Strain at failure and c) Tensile failure. Subfigures (i) represent the data with respect to the concentrations of various crosslinkers. Samples exhibiting a significant difference from the non-crosslinked sample are noted with a *, and from the Tris buffered sample are marked with a ^. Values outside brackets represent crosslinker concentration whereas values in brackets represent the ‘degree of crosslinking’ as determined by the ninhydrin free amine assay for a given condition. Subfigures (ii) summarise the data as a function of the ’degree of crosslinking’.
    Figure Legend Snippet: Mechanical characterisation of collagen films crosslinked with EDC-NHS, genipin and TG2: a) Tensile modulus b) Strain at failure and c) Tensile failure. Subfigures (i) represent the data with respect to the concentrations of various crosslinkers. Samples exhibiting a significant difference from the non-crosslinked sample are noted with a *, and from the Tris buffered sample are marked with a ^. Values outside brackets represent crosslinker concentration whereas values in brackets represent the ‘degree of crosslinking’ as determined by the ninhydrin free amine assay for a given condition. Subfigures (ii) summarise the data as a function of the ’degree of crosslinking’.

    Techniques Used: Concentration Assay

    9) Product Images from "Modular Small Diameter Vascular Grafts with Bioactive Functionalities"

    Article Title: Modular Small Diameter Vascular Grafts with Bioactive Functionalities

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0133632

    Fabrication of biomimetic tissue-engineered blood vessels (bTEBV). ( A ) The scaffold is composed of the negatively charged inert polymers, N , O -carboxymethyl chitosan ( N , O -CMC) and alginate. This scaffold is enriched with the bioactive polyanionic polyP, as well as gelatin. The hydrogel is pressed through an extruder and immediately submersed in a solution, containing the cation Ca 2+ , followed by hardening of the material. The Ca 2+ can be partially substituted by polycations, e.g. poly( l -Lys), or the peptide His/Gly-tagged RGD. After addition of poly( l -Lys), especially together with the His/Gly-tagged RGD peptide, the endothelial cells densely cover the surface of the hydrogel. The outer cell layer, composed of mural cells, is only sketched for completeness, but not studied here. ( B to D ) Human, umbilical vein/vascular endothelial cells, EA.HY926 cells, grown for 2 weeks on the following scaffold matrices; ( B ) basic scaffold alone, ( C ) basic scaffold, supplemented with poly( d -Lys), or ( C ) basic scaffold, supplemented with His/Gly-tagged RGD. The cells are stained, after fixation, with DRAQ5 (blue fluorescence) and labeled antibodies against actin (red), as described under “Material and methods”.
    Figure Legend Snippet: Fabrication of biomimetic tissue-engineered blood vessels (bTEBV). ( A ) The scaffold is composed of the negatively charged inert polymers, N , O -carboxymethyl chitosan ( N , O -CMC) and alginate. This scaffold is enriched with the bioactive polyanionic polyP, as well as gelatin. The hydrogel is pressed through an extruder and immediately submersed in a solution, containing the cation Ca 2+ , followed by hardening of the material. The Ca 2+ can be partially substituted by polycations, e.g. poly( l -Lys), or the peptide His/Gly-tagged RGD. After addition of poly( l -Lys), especially together with the His/Gly-tagged RGD peptide, the endothelial cells densely cover the surface of the hydrogel. The outer cell layer, composed of mural cells, is only sketched for completeness, but not studied here. ( B to D ) Human, umbilical vein/vascular endothelial cells, EA.HY926 cells, grown for 2 weeks on the following scaffold matrices; ( B ) basic scaffold alone, ( C ) basic scaffold, supplemented with poly( d -Lys), or ( C ) basic scaffold, supplemented with His/Gly-tagged RGD. The cells are stained, after fixation, with DRAQ5 (blue fluorescence) and labeled antibodies against actin (red), as described under “Material and methods”.

    Techniques Used: Staining, Fluorescence, Labeling

    Influence of the different additives to the universal, basic scaffold on cell growth/viability. The scaffold is prepared of N , O -CMC, silica, polyP and alginate/gelatin in a fixed sequence. The adhesion-promoting oligo/polymers, the polycationic poly- l -lysine and poly- d -lysine as well as His/Gly-tagged RGD were incorporated into the scaffold as described under “Material and methods”. The biomaterial was printed to 0.95 to 1.0 cm discs and placed into 48 well plates. After an incubation period of 7 d the growth/viability of the cells was determined using the XTT assay and the absorbance was measured at 490 nm. Both the “control-scaffold” (cont-sca) and the bioactive scaffolds “poly( l -Lys)-scaffold” (poly( l -Lys)-sca), “poly( d -Lys)-scaffold” (poly( d -Lys)-sca) and “RGD-scaffold” (RGD-sca) were examined. After an incubation period of 7 d the amount of insoluble tetrazolium salt formed was determined. The standard errors of the means (SEM) are indicated (n = 10 experiments); * P
    Figure Legend Snippet: Influence of the different additives to the universal, basic scaffold on cell growth/viability. The scaffold is prepared of N , O -CMC, silica, polyP and alginate/gelatin in a fixed sequence. The adhesion-promoting oligo/polymers, the polycationic poly- l -lysine and poly- d -lysine as well as His/Gly-tagged RGD were incorporated into the scaffold as described under “Material and methods”. The biomaterial was printed to 0.95 to 1.0 cm discs and placed into 48 well plates. After an incubation period of 7 d the growth/viability of the cells was determined using the XTT assay and the absorbance was measured at 490 nm. Both the “control-scaffold” (cont-sca) and the bioactive scaffolds “poly( l -Lys)-scaffold” (poly( l -Lys)-sca), “poly( d -Lys)-scaffold” (poly( d -Lys)-sca) and “RGD-scaffold” (RGD-sca) were examined. After an incubation period of 7 d the amount of insoluble tetrazolium salt formed was determined. The standard errors of the means (SEM) are indicated (n = 10 experiments); * P

    Techniques Used: Sequencing, Incubation, XTT Assay

    Density of EA.HY926 cells grown on different scaffolds for 2 weeks. Left panel : staining with DRAQ5; right panel : staining for the cytoskeleton structures, actin; the fluorescent immunostained cells are shown. Density of the cells ( A and B ) onto “control-scaffold”, ( C and D ) onto “poly( d -Lys)-scaffold”, ( E and F ) onto “poly( l -Lys)-scaffold”, ( G and H ) onto “RGD-scaffold” and finally ( I and J ) onto a scaffold containing both poly( l -Lys) and His/Gly-tagged RGD.
    Figure Legend Snippet: Density of EA.HY926 cells grown on different scaffolds for 2 weeks. Left panel : staining with DRAQ5; right panel : staining for the cytoskeleton structures, actin; the fluorescent immunostained cells are shown. Density of the cells ( A and B ) onto “control-scaffold”, ( C and D ) onto “poly( d -Lys)-scaffold”, ( E and F ) onto “poly( l -Lys)-scaffold”, ( G and H ) onto “RGD-scaffold” and finally ( I and J ) onto a scaffold containing both poly( l -Lys) and His/Gly-tagged RGD.

    Techniques Used: Staining

    10) Product Images from "Adipose-derived Stem/Stromal Cells on Electrospun Fibrin Microfiber Bundles Enable Moderate Muscle Reconstruction in a Volumetric Muscle Loss Model"

    Article Title: Adipose-derived Stem/Stromal Cells on Electrospun Fibrin Microfiber Bundles Enable Moderate Muscle Reconstruction in a Volumetric Muscle Loss Model

    Journal: Cell Transplantation

    doi: 10.1177/0963689718805370

    ASC growth and myogenesis on electrospun fibrin fibers. (A) Live (green)/Dead (red) staining of cells seeded at 40 000 cells/μl at days 0 and 21. (B) Histological cross-section stained with DAPI (blue) showing ASC infiltration to the fiber interior at day 21. (C) Quantification of total DNA content of fibers seeded with ASCs at the three seeding densities at days 0, 7, 14, and 21. *p
    Figure Legend Snippet: ASC growth and myogenesis on electrospun fibrin fibers. (A) Live (green)/Dead (red) staining of cells seeded at 40 000 cells/μl at days 0 and 21. (B) Histological cross-section stained with DAPI (blue) showing ASC infiltration to the fiber interior at day 21. (C) Quantification of total DNA content of fibers seeded with ASCs at the three seeding densities at days 0, 7, 14, and 21. *p

    Techniques Used: Staining

    11) Product Images from "Regulating in vivo calcification of alginate microbeads"

    Article Title: Regulating in vivo calcification of alginate microbeads

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2010.03.001

    XRD. Spectra of HEPES powder used to buffer the crosslinking solution, alginate powder used to make the microbeads, buffered microbeads after 5 weeks in vivo , NaCl from the database, non-buffered microbeads after 5 weeks in vivo , and hydroxyapatite from the database.
    Figure Legend Snippet: XRD. Spectra of HEPES powder used to buffer the crosslinking solution, alginate powder used to make the microbeads, buffered microbeads after 5 weeks in vivo , NaCl from the database, non-buffered microbeads after 5 weeks in vivo , and hydroxyapatite from the database.

    Techniques Used: In Vivo

    FTIR. Spectra of (A) non-buffered microbeads after 5 weeks in vivo , (B) buffered microbeads after 5 weeks in vivo , (C) HEPES powder used to buffer the crosslinking solution, and (D) alginate powder used to make the microbeads.
    Figure Legend Snippet: FTIR. Spectra of (A) non-buffered microbeads after 5 weeks in vivo , (B) buffered microbeads after 5 weeks in vivo , (C) HEPES powder used to buffer the crosslinking solution, and (D) alginate powder used to make the microbeads.

    Techniques Used: In Vivo

    12) Product Images from "Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers"

    Article Title: Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-01581-y

    Coaxial 3D bioprinting system and the tumor fiber fabrication process. ( A ) Coaxial 3D bioprinting system contains a XYZ three-axis platform, a syringe pump, a coaxial nozzle and the computer-controlled system. ( B ) Different styles of the printing nozzles adapted with this bioprinting platform. ( C ) The schematic of the biofabrication process of multicellular heterogeneous tumor fiber: coaxial bioprinting, in vitro culturing and de-crosslinking.
    Figure Legend Snippet: Coaxial 3D bioprinting system and the tumor fiber fabrication process. ( A ) Coaxial 3D bioprinting system contains a XYZ three-axis platform, a syringe pump, a coaxial nozzle and the computer-controlled system. ( B ) Different styles of the printing nozzles adapted with this bioprinting platform. ( C ) The schematic of the biofabrication process of multicellular heterogeneous tumor fiber: coaxial bioprinting, in vitro culturing and de-crosslinking.

    Techniques Used: In Vitro

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    Article Title: Dual-Phase Osteogenic and Vasculogenic Engineered Tissue for Bone Formation
    Article Snippet: .. Microbeads were cultured and maintained in α-MEM supplemented with 2.0 mg/mL ɛ-amino caproic acid (ACA; Sigma) to prevent fibrinolysis. .. Acellular microbeads were stained with EZBlue Coomasie reagent and visualized with a light microscope (Olympus America).

    Article Title: Single-step laser-based fabrication and patterning of cell-encapsulated alginate microbeads
    Article Snippet: .. To prepare alginate for cross-linking into microbeads, alginic acid sodium salt (Sigma Aldrich, St. Louis, MO) was dissolved slowly into cell culture grade distilled water at 2% w/v along with sodium chloride at 0.9% w/v. ..