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fipv3 70  (Novus Biologicals)


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    Novus Biologicals fipv3 70
    Fipv3 70, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/coronavirus+antibody+(fipv3-70)+-+bsa+free/Coronavirus+Antibody+(FIPV3-70)+-+BSA+Free/pmc11768839-94-20-21
    Average 92 stars, based on 5 article reviews
    fipv3 70 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Expressing:

    Article Title: Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals
    Article Snippet: Adenovirus E1A and hexon proteins were detected using rabbit α-E1A (Santa Cruz sc-430) and undiluted hybridoma (2Hx-2) media, respectively, as previously detailed [ ]. .. To measure the effect of the compound on HCoV-229E, HCoV-OC43, or SARS-CoV-2 nucleocapsid (N) protein expression, mouse α-coronavirus antibody clone FIPV3-70 (Novus Biologicals, Cat: NB10064754), mouse α-coronavirus group antigen antibody clone 542-7D (Millipore Sigma, Cat: MAB9013), or rabbit anti-SARS-CoV-2 nucleocapsid antibody (SinoBiological, Cat: 40143-R019) were used, respectively. .. Changes in HCoV-OC43 spike protein expression were assessed using mouse monoclonal antibody 541-8F (Sigma-Aldrich, MAB9012).

    Article Title: Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals
    Article Snippet: Adenovirus E1A and hexon proteins were detected using rabbit α-E1A (Santa Cruz sc-430) and undiluted hybridoma (2Hx-2) media, respectively, as previously detailed . .. To measure the effect of compound on HCoV-229E, HCoV-OC43, or SARS CoV-2 nucleocapsid (N) protein expression, mouse α-coronavirus antibody clone FIPV3-70 (Novus Biologicals, Cat: NB10064754), mouse α-coronavirus group antigen antibody clone 542-7D (Millipore Sigma, Cat: MAB9013), or rabbit anti-SARS CoV2 nucleocapsid antibody (SinoBiological, Cat: 40143-R019) were used, respectively. .. Changes in HCoV-OC43 spike protein expression were assessed using mouse monoclonal antibody 541-8F (Sigma-Aldrich, MAB9012).

    other:

    Article Title: On a path toward a broad-spectrum anti-viral: inhibition of HIV-1 and coronavirus replication by SR kinase inhibitor harmine
    Article Snippet: Type Size (kDa) Dilution Company Catalog No. Gag p24 Ms 55, 41, 24 1:500 in 1X TBST NIH AIDS Reagent #1513 Env gp41 Hu 160 1:1000 in 1X TBST NIH AIDS Reagent #531 Tat 1D9 Ms 16, 14 1:300 in 1X TBST NIH AIDS Reagent #7383 DYRK1A Rb 90 1:1000 in 1 X TBST Cell Signaling Technologies #8765S GAPDH Rb 35.8 1:3000 in 1X TBST Sigma-Aldrich G9545 Tubulin Rb 55 1:3000 in 1 X TBST Cell Signaling Technologies #2144S CLK1 Ms 60 1:1000 in 5% BSA-TBST Santa Cruz #515897 CLK2 Rb 60 1:1000 in 5% BSA-TBST Abcam ab65082 CLK3 Rb 60 1:1000 in 5% BSA-TBST Abnova H00001198-M05 SRPK1 Rb 110 1:2000 in 5% BSA-TBST Cedarlane OAAN01583 SRSF1 (ASF/SF2) Ms 32 1:1000 in 1X TBST Life Technologies 32-4500 SRSF2 (SC35) Rb ~35 1:1000 in 1X TBST Abcam ab11826 SRSF3 (SRp20) Ms 20 1:1000 in 1X TBST Life Technologies 33-4200 SRSF4 (SRp75) Rb 75 1:2000 in 1X TBST Novus Biologicals NBP2-04144 SRSF5 Rb ~40 1:1000 in 1X TBST MBL RN082PW SRSF6 (SRp55) Rb 55 1:2000 in 1X TBST Novus Biologicals NBP2-04142 SRSF7 (9G8) Rb ~35 1:2000 in 1X TBST Abcam ab137247 SRSF9 (SRp30c) Rb 27 1:1000 in 1X TBST MBL RN081PW SRSF10 (FUSIP1) Ms 37 1:1000 in 1X TBST Novus Biologicals H00010772-M07 Tra2ß Rb ~40 1:1000 in 1X TBST Abcam ab31353 Feline anti-coronavirus antibody (FIPV3-70 for HCoV-229E N) Ms ~60 1:4000 in 1 X TBST Novus Biologicals NB10064754

    Staining:

    Article Title: Targeting protein homeostasis with small molecules as a strategy for the development of pan-coronavirus antiviral therapies
    Article Snippet: .. Blots were stained with anti-229E N (1:5000, Novus Biologicals NB10064754), Sars-CoV-2 N (1:1000, ThermoFisher Scientific MA5-29981), AMPKα [1:500, Cell Signaling Technology (CST) 2532S], Phospho-AMPKα (Thr172) (1:500, CST 2535S), mTOR (1:1000, CST 2983S), Phospho-mTOR (Ser2448) (1:500, CST 5536S), Akt (pan) (1:2000, CST 4691S), Phospho-Akt (Ser473) (1:1000, CST 4060S), and GAPDH (1:20000, Abcam ab8245) for loading control. ..

    Control:

    Article Title: Targeting protein homeostasis with small molecules as a strategy for the development of pan-coronavirus antiviral therapies
    Article Snippet: .. Blots were stained with anti-229E N (1:5000, Novus Biologicals NB10064754), Sars-CoV-2 N (1:1000, ThermoFisher Scientific MA5-29981), AMPKα [1:500, Cell Signaling Technology (CST) 2532S], Phospho-AMPKα (Thr172) (1:500, CST 2535S), mTOR (1:1000, CST 2983S), Phospho-mTOR (Ser2448) (1:500, CST 5536S), Akt (pan) (1:2000, CST 4691S), Phospho-Akt (Ser473) (1:1000, CST 4060S), and GAPDH (1:20000, Abcam ab8245) for loading control. ..



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    Image Search Results


    Patient Demographics

    Journal: The Journal of Heart and Lung Transplantation

    Article Title: Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection

    doi: 10.1016/j.healun.2019.12.009

    Figure Lengend Snippet: Patient Demographics

    Article Snippet: 20S proteasome subunit α3 (sc-58414, Santa Cruz Biotechnology), rhinovirus VP3 (MA5-18249, Thermo Fisher Scientific, Waltham, MA), coronavirus (NB100-64754, Novus Biologicals, Littleton, CO), and RSV glycoprotein G (7950-0980, Bio-Rad Laboratories, Hercules, CA) were used as primary Abs; secondary Abs conjugated with horseradish peroxidase (HRP) were used specific to primary Ab.

    Techniques: Infection

    Lung-associated SAgs and viral antigens were demonstrable in exosomes isolated from patients with RVI. Exosomes isolated from serum samples of patients with RVI and from stable LTxRs were used to detect the presence of lung-associated SAgs and viral antigens using immunoblot. The results showed a significant increase in lung-associated antigens and viral antigens. ( A ) RSV ( n = 10), coronavirus ( n = 12), and rhinovirus ( n = 12) in respective patients with viral infection compared with stable LTxRs ( n = 30). ( B ) Graphical representation shows the optical density of lung-associated SAgs and viral antigens measured in RVI and stable LTxRs using ImageJ software. Optical density of lung SAgs and viral antigens were normalized with exosomes specific marker CD9. The presence of lung SAgs and viral antigens in the exosomes was compared between the cohorts using Mann–Whitney test. Asterisk indicates statistically significant. Col-V, collagen-V; CV, coronavirus; Kα1T, K-alpha-1 tubulin; LTxR, lung transplant recipient; RSV, respiratory syncytial virus; RV, rhinovirus; RVI, respiratory viral infection; SAg, self-antigen.

    Journal: The Journal of Heart and Lung Transplantation

    Article Title: Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection

    doi: 10.1016/j.healun.2019.12.009

    Figure Lengend Snippet: Lung-associated SAgs and viral antigens were demonstrable in exosomes isolated from patients with RVI. Exosomes isolated from serum samples of patients with RVI and from stable LTxRs were used to detect the presence of lung-associated SAgs and viral antigens using immunoblot. The results showed a significant increase in lung-associated antigens and viral antigens. ( A ) RSV ( n = 10), coronavirus ( n = 12), and rhinovirus ( n = 12) in respective patients with viral infection compared with stable LTxRs ( n = 30). ( B ) Graphical representation shows the optical density of lung-associated SAgs and viral antigens measured in RVI and stable LTxRs using ImageJ software. Optical density of lung SAgs and viral antigens were normalized with exosomes specific marker CD9. The presence of lung SAgs and viral antigens in the exosomes was compared between the cohorts using Mann–Whitney test. Asterisk indicates statistically significant. Col-V, collagen-V; CV, coronavirus; Kα1T, K-alpha-1 tubulin; LTxR, lung transplant recipient; RSV, respiratory syncytial virus; RV, rhinovirus; RVI, respiratory viral infection; SAg, self-antigen.

    Article Snippet: 20S proteasome subunit α3 (sc-58414, Santa Cruz Biotechnology), rhinovirus VP3 (MA5-18249, Thermo Fisher Scientific, Waltham, MA), coronavirus (NB100-64754, Novus Biologicals, Littleton, CO), and RSV glycoprotein G (7950-0980, Bio-Rad Laboratories, Hercules, CA) were used as primary Abs; secondary Abs conjugated with horseradish peroxidase (HRP) were used specific to primary Ab.

    Techniques: Isolation, Western Blot, Infection, Software, Marker, MANN-WHITNEY, Virus