calcein am  (Thermo Fisher)


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    Name:
    Calcein AM
    Description:
    Calcein AM is a cell permeant dye that can be used to determine cell viability in most eukaryotic cells In live cells the nonfluorescent calcein AM is converted to a green fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases This dye is also available in our special packaging C 3100 and as 1 mg of the solid C 1430 For a longer wavelength version of this dye check out our new CellTrace calcein red orange AM Cat no C 34851
    Catalog Number:
    c3099
    Price:
    None
    Applications:
    Cell Adhesion|Cell Analysis|Cell Counting & Viability|Cell Counting & Viability|Cell Culture|Cell Proliferation|Cell Viability & Cytotoxicity|Cellular Imaging|Cellular Toxicology Assays|Chemotaxis & Cell Migration|Chemotaxis & Cell Migration|General Cell Tracing|Glutathione Detection|High-Content Screening (HCS)|Immunofluorescence (IF)|Immunofluorescence Staining & Detection|Industrial & Applied Science|Ionic Homeostasis & Signaling|Microbial Tracking|Nitro-Oxidative Stress|Pharma & Biopharma|Target-Based ADME⁄Tox Assays|Zinc & Heavy Metal Ion Detection|Cell Tracing & Tracking|Cell Viability, Proliferation & Function|Cell Counting, Viability, & Cryopreservation
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher calcein am
    Polydatin (PD) alleviates TNF‐α‐induced mitochondrial dysfunction in NPCs. After 2 h of pretreatment with PD, TNF‐α was added into culture media at the concentration of 50 ng/mL and NPCs were incubated for another 72 h. A, B, MitoSOX assay showed that PD reversed the ROS accumulation in TNF‐α‐treated NPCs. Scale bar: 50 μm. C, Intracellular MDA level was assessed by MDA Assay Kit. D, E, The effect of PD on mitochondrial potential in NPCs treated with TNF‐α using <t>MitoTracker</t> Green. Scale bar: 10 μm. F, Intracellular ATP level was assessed by ATP‐Glo Bioluminometric Cell Viability Assay. The values presented are the means ± SD of three independent experiments. * P
    Calcein AM is a cell permeant dye that can be used to determine cell viability in most eukaryotic cells In live cells the nonfluorescent calcein AM is converted to a green fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases This dye is also available in our special packaging C 3100 and as 1 mg of the solid C 1430 For a longer wavelength version of this dye check out our new CellTrace calcein red orange AM Cat no C 34851
    https://www.bioz.com/result/calcein am/product/Thermo Fisher
    Average 90 stars, based on 924 article reviews
    Price from $9.99 to $1999.99
    calcein am - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Polydatin suppresses nucleus pulposus cell senescence, promotes matrix homeostasis and attenuates intervertebral disc degeneration in rats, et al. Polydatin suppresses nucleus pulposus cell senescence, promotes matrix homeostasis and attenuates intervertebral disc degeneration in rats"

    Article Title: Polydatin suppresses nucleus pulposus cell senescence, promotes matrix homeostasis and attenuates intervertebral disc degeneration in rats, et al. Polydatin suppresses nucleus pulposus cell senescence, promotes matrix homeostasis and attenuates intervertebral disc degeneration in rats

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.13848

    Polydatin (PD) alleviates TNF‐α‐induced mitochondrial dysfunction in NPCs. After 2 h of pretreatment with PD, TNF‐α was added into culture media at the concentration of 50 ng/mL and NPCs were incubated for another 72 h. A, B, MitoSOX assay showed that PD reversed the ROS accumulation in TNF‐α‐treated NPCs. Scale bar: 50 μm. C, Intracellular MDA level was assessed by MDA Assay Kit. D, E, The effect of PD on mitochondrial potential in NPCs treated with TNF‐α using MitoTracker Green. Scale bar: 10 μm. F, Intracellular ATP level was assessed by ATP‐Glo Bioluminometric Cell Viability Assay. The values presented are the means ± SD of three independent experiments. * P
    Figure Legend Snippet: Polydatin (PD) alleviates TNF‐α‐induced mitochondrial dysfunction in NPCs. After 2 h of pretreatment with PD, TNF‐α was added into culture media at the concentration of 50 ng/mL and NPCs were incubated for another 72 h. A, B, MitoSOX assay showed that PD reversed the ROS accumulation in TNF‐α‐treated NPCs. Scale bar: 50 μm. C, Intracellular MDA level was assessed by MDA Assay Kit. D, E, The effect of PD on mitochondrial potential in NPCs treated with TNF‐α using MitoTracker Green. Scale bar: 10 μm. F, Intracellular ATP level was assessed by ATP‐Glo Bioluminometric Cell Viability Assay. The values presented are the means ± SD of three independent experiments. * P

    Techniques Used: Concentration Assay, Incubation, Mitosox Assay, Multiple Displacement Amplification, Viability Assay

    Knockdown of Nrf2 compromises the antisenescence, anti‐ECM disorder and antimitochondrial dysfunction properties of PD on NPCs. A‐C, Successful knockdown of Nrf2 suppressed the expression level of HO‐1. D, E, The expression of p53 and p16 in NPCs treated with or without pretreatment of PD for 2 h and another administration of TNF‐α for 72 h. F, G, The levels of ECM‐related proteins such as aggrecan, MMP‐13 and MMP‐3 were detected by Western blotting. H‐J, Representative images for MitoSOX and MitoTracker of PD and TNF‐α‐treated NPCs with or without Nrf2 knockdown. Scale bar: 50 μm. Data presented are the means ± SD of three independent experiments. * P
    Figure Legend Snippet: Knockdown of Nrf2 compromises the antisenescence, anti‐ECM disorder and antimitochondrial dysfunction properties of PD on NPCs. A‐C, Successful knockdown of Nrf2 suppressed the expression level of HO‐1. D, E, The expression of p53 and p16 in NPCs treated with or without pretreatment of PD for 2 h and another administration of TNF‐α for 72 h. F, G, The levels of ECM‐related proteins such as aggrecan, MMP‐13 and MMP‐3 were detected by Western blotting. H‐J, Representative images for MitoSOX and MitoTracker of PD and TNF‐α‐treated NPCs with or without Nrf2 knockdown. Scale bar: 50 μm. Data presented are the means ± SD of three independent experiments. * P

    Techniques Used: Expressing, Western Blot

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    Positive Control:

    Article Title: Biological and Pro-Angiogenic Properties of Genetically Modified Human Primary Myoblasts Overexpressing Placental Growth Factor in In Vitro and In Vivo Studies
    Article Snippet: As a negative control, DMEM and fresh myoblast media were used; as a positive control, Medium 200 supplemented with LVES was used. .. Afterwards, the newly formed capillaries were stained with 2 μg/ml calcein-AM (Invitrogen, Carlsbad, USA).

    Cytometry:

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    Article Snippet: Viable target (T) lymphocytes were stained with Calcein-AM (CAM; Molecular Probes, Eugene, OR) 100 n m (30 min incubation at 37°C). .. After washing the cells three times in PBS, their staining pattern was analysed using a Becton Dickinson FACSort flow cytometer interfaced to a Hewlett Packard computer.

    Immunostaining:

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    Article Snippet: Paragraph title: 7. Immunostaining and Viability Test in Microchannels ... A Live/Dead Viability test was performed by use of Calcein AM (Molecular probes, C3099) and Propidium Iodide (Molecular Probes, V13245) according to the manufacturer’s protocols provided with the products.

    Adsorption:

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    Article Snippet: To prevent any nonspecific antibody adsorption cells were incubated with 0.1 BSA and 10% goat serum for 1 hour. .. A Live/Dead Viability test was performed by use of Calcein AM (Molecular probes, C3099) and Propidium Iodide (Molecular Probes, V13245) according to the manufacturer’s protocols provided with the products.

    Incubation:

    Article Title: Quantifying Molecular-Level Cell Adhesion on Electroactive Conducting Polymers using Electrochemical-Single Cell Force Spectroscopy
    Article Snippet: Calcein Staining of L929 Cells The live cell stain was stored as 1 mM Calcein AM (C3100MP, Life Technology) in DMSO(C-3099, Life Technology). .. The calcein AM solution replaced the culture media and live cells were incubated for 15 minutes at 37 °C.

    Article Title: Biological and Pro-Angiogenic Properties of Genetically Modified Human Primary Myoblasts Overexpressing Placental Growth Factor in In Vitro and In Vivo Studies
    Article Snippet: The HUVECs were further incubated for 14–18 h with the supernatants harvested from myoblasts. .. Afterwards, the newly formed capillaries were stained with 2 μg/ml calcein-AM (Invitrogen, Carlsbad, USA).

    Article Title: Facile Bench-Top Fabrication of Enclosed Circular Microchannels Provides 3D Confined Structure for Growth of Prostate Epithelial Cells
    Article Snippet: Cultures were then incubated with anti-mouse Cy5 (Jackson, dilution 1/500 in PBS+ , Tween 20 0.05%, and 5% goat serum). .. A Live/Dead Viability test was performed by use of Calcein AM (Molecular probes, C3099) and Propidium Iodide (Molecular Probes, V13245) according to the manufacturer’s protocols provided with the products.

    Article Title: Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
    Article Snippet: Cells were incubated for 24 hours with AngII (10–7 M), losartan (10–6 M), or peritoneal macrophages from renin+/+ or renin–/– mice placed in the lower chamber. .. Briefly, THP-1 cells were stimulated by TNF-α (10 ng/ml) and then labeled with calcein AM (catalog no. C-3099; Invitrogen).

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    Article Title: Infused polymers for cell sheet release
    Article Snippet: .. Transferred cells were washed with HBSS twice and then incubated in 2uM Calcein AM (Molecular Probes, C3099) and 2uM Sytox Orange (Molecular Probes, S11368) in Serum-free DMEM at 37 °C for 1 h. The stained cells were then washed twice with HBSS and imaged in Live Cell Imaging Buffer (Molecular Probes, A1429DJ). .. Cells growing on infused PDMS (state of cells before detachment) and cells growing on TCPS were also stained and imaged as controls.

    Article Title: PSMA-homing dsRNA chimeric protein vector kills prostate cancer cells and activates anti-tumor bystander responses
    Article Snippet: LNCaP or LNCaP-Luc/GFP cells were seeded (2000 cells per well) and incubated at 37 °C in 5% CO2 . .. On Day 15, spheroids were stained with calcein AM (1:1000, c3099, Molecular Probes, Eugene, OR, USA) and 0.5μg/ml propidium iodide.

    Article Title: Mechanisms of Mycobacterium avium-induced resistance against insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice: role of Fas and Th1 cells
    Article Snippet: .. Viable target (T) lymphocytes were stained with Calcein-AM (CAM; Molecular Probes, Eugene, OR) 100 n m (30 min incubation at 37°C). .. E and T cells were cultured together in RPMI 1640 medium (G ibco BRL Life Technologies Ltd) containing 10% FCS, 200 m m l -glutamine, 10 m m HEPES (all from G ibco BRL) and 50 μ m 2-mercaptoethanol (2-ME; Sigma), for 3 h (37°C, 5% CO2 ).

    Activity Assay:

    Article Title: Infused polymers for cell sheet release
    Article Snippet: Cell Sheet Viability To determine the viability of the cell sheets after transfer, Calcein AM was used to detect intracellular esterase activity and Sytox Orange Nucleic Acid stain to detect membrane integrity. .. Transferred cells were washed with HBSS twice and then incubated in 2uM Calcein AM (Molecular Probes, C3099) and 2uM Sytox Orange (Molecular Probes, S11368) in Serum-free DMEM at 37 °C for 1 h. The stained cells were then washed twice with HBSS and imaged in Live Cell Imaging Buffer (Molecular Probes, A1429DJ).

    Infection:

    Article Title: Mechanisms of Mycobacterium avium-induced resistance against insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice: role of Fas and Th1 cells
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    Modification:

    Article Title: Sequentially Moldable and Bondable 4D Hydrogels Compatible with Cell Encapsulation
    Article Snippet: .. Minimum Essential Medium Eagle-Alpha Modification (αMEM; ref. 12000014), fetal bovine serum (FBS; ref. 10270106), Alamar Blue reagent (ref. DAL1025), Calcein AM (ref. C3099) and propidium iodide (ref. P1304MP), TrypLe™ Express Enzyme (ref. 12605010) and penicillin/streptomycin solution (ref. 15140122) were acquired from ThermoScientific. .. A solution of CHT was prepared using MMW chitosan dissolved at 0.5 mg mL-1 in a solution of 1% (v/v) glacial acetic acid (Sigma) in distilled water.

    Western Blot:

    Article Title: Epigenetic Modification of MicroRNA-200b Contributes to Diabetic Vasculopathy
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    Flow Cytometry:

    Article Title: Mechanisms of Mycobacterium avium-induced resistance against insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice: role of Fas and Th1 cells
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    Cell Culture:

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    Article Title: Sequentially Moldable and Bondable 4D Hydrogels Compatible with Cell Encapsulation
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    Article Title: Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
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    Article Title: Mechanisms of Mycobacterium avium-induced resistance against insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice: role of Fas and Th1 cells
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    Purification:

    Article Title: In vivo histone H1 migration from necrotic to viable tissue
    Article Snippet: All other H1s were purified by Dr. Missag Parseghian. .. Calcein AM (Cat. # C3099) was from Life Technologies.

    Article Title: Epigenetic Modification of MicroRNA-200b Contributes to Diabetic Vasculopathy
    Article Snippet: Purified Rat Anti-Mouse CD31 (also known as PECAM-1) (cat. no. 550274) was obtained from BD PharMingen (San Jose, CA, USA). .. Calcein AM (cat. no. C3099) was purchased from Molecular Probes, Thermo Fisher Scientific (Waltham, MA, USA).

    other:

    Article Title: Hemocompatibility of Hyaluronan Enhanced Linear Low Density Polyethylene for Blood Contacting Applications
    Article Snippet: 1ml of a 5µM solution of Calcein-AM in PBS was added to the surfaces.

    Imaging:

    Article Title: Bifidobacterium dentium Fortifies the Intestinal Mucus Layer via Autophagy and Calcium Signaling Pathways
    Article Snippet: Cells were placed in an Okolabs stage-top incubation chamber with CO2 mixing and humidity control and placed on an Nikon TiE inverted wide-field epifluorescence microscope with a motorized X, Y, and Z stage for software-controlled multiposition imaging. .. Following the experiment, cell viability was examined by staining cells with 3 μM calcein AM (live-cell dye, catalog no. C3099; Invitrogen) and 2.5 μM propidium iodide (dead-cell dye, catalog no. P4170; Sigma-Aldrich).

    Fluorescence:

    Article Title: Fenofibrate Interferes with the Diapedesis of Lung Adenocarcinoma Cells through the Interference with Cx43/EGF-Dependent Intercellular Signaling
    Article Snippet: Acceptor HUVEC cells grown in Petri dishes were pre-incubated with FF (25 μM) for 4 h and calcein (Life Technologies, C3099)-loaded donor A549 cells/HUVECs were seeded onto the monolayers of HUVEC cells. .. After 1 h, calcein transfer from donor to acceptor cells was evaluated using a Leica DM IRE2 inverted fluorescence microscope.

    Article Title: Fenofibrate Interferes with the Diapedesis of Lung Adenocarcinoma Cells through the Interference with Cx43/EGF-Dependent Intercellular Signaling
    Article Snippet: Calcein Transfer Assay Acceptor HUVEC cells grown in Petri dishes were pre-incubated with FF (25 μM) for 4 h and calcein (Life Technologies, C3099)-loaded donor A549 cells/HUVECs were seeded onto the monolayers of HUVEC cells. .. After 1 h, calcein transfer from donor to acceptor cells was evaluated using a Leica DM IRE2 inverted fluorescence microscope.

    Isolation:

    Article Title: Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
    Article Snippet: Adhesion assay HUVECs were isolated from human umbilical cords obtained from the University of Kentucky Labor and Delivery unit and cultured in M199 media supplemented with 20% FBS as described previously ( ). .. Briefly, THP-1 cells were stimulated by TNF-α (10 ng/ml) and then labeled with calcein AM (catalog no. C-3099; Invitrogen).

    Negative Control:

    Article Title: Biological and Pro-Angiogenic Properties of Genetically Modified Human Primary Myoblasts Overexpressing Placental Growth Factor in In Vitro and In Vivo Studies
    Article Snippet: As a negative control, DMEM and fresh myoblast media were used; as a positive control, Medium 200 supplemented with LVES was used. .. Afterwards, the newly formed capillaries were stained with 2 μg/ml calcein-AM (Invitrogen, Carlsbad, USA).

    Microscopy:

    Article Title: Fenofibrate Interferes with the Diapedesis of Lung Adenocarcinoma Cells through the Interference with Cx43/EGF-Dependent Intercellular Signaling
    Article Snippet: Acceptor HUVEC cells grown in Petri dishes were pre-incubated with FF (25 μM) for 4 h and calcein (Life Technologies, C3099)-loaded donor A549 cells/HUVECs were seeded onto the monolayers of HUVEC cells. .. After 1 h, calcein transfer from donor to acceptor cells was evaluated using a Leica DM IRE2 inverted fluorescence microscope.

    Article Title: Fenofibrate Interferes with the Diapedesis of Lung Adenocarcinoma Cells through the Interference with Cx43/EGF-Dependent Intercellular Signaling
    Article Snippet: Calcein Transfer Assay Acceptor HUVEC cells grown in Petri dishes were pre-incubated with FF (25 μM) for 4 h and calcein (Life Technologies, C3099)-loaded donor A549 cells/HUVECs were seeded onto the monolayers of HUVEC cells. .. After 1 h, calcein transfer from donor to acceptor cells was evaluated using a Leica DM IRE2 inverted fluorescence microscope.

    Article Title: Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
    Article Snippet: Briefly, THP-1 cells were stimulated by TNF-α (10 ng/ml) and then labeled with calcein AM (catalog no. C-3099; Invitrogen). .. Bound monocytes were counted by an inverted phase-contrast microscope.

    Article Title: Bifidobacterium dentium Fortifies the Intestinal Mucus Layer via Autophagy and Calcium Signaling Pathways
    Article Snippet: Cells were placed in an Okolabs stage-top incubation chamber with CO2 mixing and humidity control and placed on an Nikon TiE inverted wide-field epifluorescence microscope with a motorized X, Y, and Z stage for software-controlled multiposition imaging. .. Following the experiment, cell viability was examined by staining cells with 3 μM calcein AM (live-cell dye, catalog no. C3099; Invitrogen) and 2.5 μM propidium iodide (dead-cell dye, catalog no. P4170; Sigma-Aldrich).

    Article Title: Infused polymers for cell sheet release
    Article Snippet: Transferred cells were washed with HBSS twice and then incubated in 2uM Calcein AM (Molecular Probes, C3099) and 2uM Sytox Orange (Molecular Probes, S11368) in Serum-free DMEM at 37 °C for 1 h. The stained cells were then washed twice with HBSS and imaged in Live Cell Imaging Buffer (Molecular Probes, A1429DJ). .. A Zeiss LSM 710 confocal microscope was used to image the cells using the preloaded excitation and emission wavelengths set for the Calcein AM and Sytox Orange dyes.

    Mouse Assay:

    Article Title: Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
    Article Snippet: Cells were incubated for 24 hours with AngII (10–7 M), losartan (10–6 M), or peritoneal macrophages from renin+/+ or renin–/– mice placed in the lower chamber. .. Briefly, THP-1 cells were stimulated by TNF-α (10 ng/ml) and then labeled with calcein AM (catalog no. C-3099; Invitrogen).

    Article Title: Mechanisms of Mycobacterium avium-induced resistance against insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice: role of Fas and Th1 cells
    Article Snippet: A non-radioactive assay was used to determine the cytotoxic potential of splenic lymphocytes obtained from infected and control mice, as described elsewhere [ ]. .. Viable target (T) lymphocytes were stained with Calcein-AM (CAM; Molecular Probes, Eugene, OR) 100 n m (30 min incubation at 37°C).

    Cell Adhesion Assay:

    Article Title: Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
    Article Snippet: Adhesion assay HUVECs were isolated from human umbilical cords obtained from the University of Kentucky Labor and Delivery unit and cultured in M199 media supplemented with 20% FBS as described previously ( ). .. Briefly, THP-1 cells were stimulated by TNF-α (10 ng/ml) and then labeled with calcein AM (catalog no. C-3099; Invitrogen).

    Labeling:

    Article Title: Renin inhibition reduces hypercholesterolemia-induced atherosclerosis in mice
    Article Snippet: .. Briefly, THP-1 cells were stimulated by TNF-α (10 ng/ml) and then labeled with calcein AM (catalog no. C-3099; Invitrogen). .. Labeled cells (5 × 104 /well) were added to the upper chamber of each well.

    Article Title: PSMA-homing dsRNA chimeric protein vector kills prostate cancer cells and activates anti-tumor bystander responses
    Article Snippet: On Day 15, spheroids were stained with calcein AM (1:1000, c3099, Molecular Probes, Eugene, OR, USA) and 0.5μg/ml propidium iodide. .. After 24 hours fresh PBMCs were labeled using 1 μM CellTracker™ Violet BMQC (Invitrogen, San Diego, CA, USA) according to the manufacturer's protocol.

    Confocal Microscopy:

    Article Title: PSMA-homing dsRNA chimeric protein vector kills prostate cancer cells and activates anti-tumor bystander responses
    Article Snippet: On Day 15, spheroids were stained with calcein AM (1:1000, c3099, Molecular Probes, Eugene, OR, USA) and 0.5μg/ml propidium iodide. .. Spheroids were monitored using confocal microscopy and size was measured using ImageJ software.

    Cytotoxicity Assay:

    Article Title: Structure–activity relationships and cellular mechanism of action of small molecules that enhance the delivery of oligonucleotides
    Article Snippet: .. A LIVE/DEAD® cytotoxicity assay kit containing Calcein-AM and ethidium dimer was purchased from ThermoFisher. ..

    Article Title: Mechanisms of Mycobacterium avium-induced resistance against insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice: role of Fas and Th1 cells
    Article Snippet: Paragraph title: Cytotoxicity assay ... Viable target (T) lymphocytes were stained with Calcein-AM (CAM; Molecular Probes, Eugene, OR) 100 n m (30 min incubation at 37°C).

    Software:

    Article Title: Bifidobacterium dentium Fortifies the Intestinal Mucus Layer via Autophagy and Calcium Signaling Pathways
    Article Snippet: Images were recorded using an ORCA-Flash 4.0 scientific complementary metal-oxide semiconductor (sCMOS) camera (Hamamatsu), and the Nikon Elements v4.5 software was used for data acquisition and image analysis. .. Following the experiment, cell viability was examined by staining cells with 3 μM calcein AM (live-cell dye, catalog no. C3099; Invitrogen) and 2.5 μM propidium iodide (dead-cell dye, catalog no. P4170; Sigma-Aldrich).

    Article Title: PSMA-homing dsRNA chimeric protein vector kills prostate cancer cells and activates anti-tumor bystander responses
    Article Snippet: On Day 15, spheroids were stained with calcein AM (1:1000, c3099, Molecular Probes, Eugene, OR, USA) and 0.5μg/ml propidium iodide. .. Spheroids were monitored using confocal microscopy and size was measured using ImageJ software.

    Functional Assay:

    Article Title: Biological and Pro-Angiogenic Properties of Genetically Modified Human Primary Myoblasts Overexpressing Placental Growth Factor in In Vitro and In Vivo Studies
    Article Snippet: The supernatants collected from the different myoblast cultures were transferred to the prepared HUVECs to evaluate the functional properties of the secreted proteins. .. Afterwards, the newly formed capillaries were stained with 2 μg/ml calcein-AM (Invitrogen, Carlsbad, USA).

    Live Cell Imaging:

    Article Title: Infused polymers for cell sheet release
    Article Snippet: .. Transferred cells were washed with HBSS twice and then incubated in 2uM Calcein AM (Molecular Probes, C3099) and 2uM Sytox Orange (Molecular Probes, S11368) in Serum-free DMEM at 37 °C for 1 h. The stained cells were then washed twice with HBSS and imaged in Live Cell Imaging Buffer (Molecular Probes, A1429DJ). .. Cells growing on infused PDMS (state of cells before detachment) and cells growing on TCPS were also stained and imaged as controls.

    Produced:

    Article Title: In vivo histone H1 migration from necrotic to viable tissue
    Article Snippet: NHS76, a fully human IgG1, was produced at Peregrine Pharmaceuticals, Inc. (Tustin, CA). .. Calcein AM (Cat. # C3099) was from Life Technologies.

    Concentration Assay:

    Article Title: Bifidobacterium dentium Fortifies the Intestinal Mucus Layer via Autophagy and Calcium Signaling Pathways
    Article Snippet: B. dentium FluoroBrite conditioned DMEM was added in uninoculated FluoroBrite DMEM (50% final concentration). .. Following the experiment, cell viability was examined by staining cells with 3 μM calcein AM (live-cell dye, catalog no. C3099; Invitrogen) and 2.5 μM propidium iodide (dead-cell dye, catalog no. P4170; Sigma-Aldrich).

    Staining:

    Article Title: Quantifying Molecular-Level Cell Adhesion on Electroactive Conducting Polymers using Electrochemical-Single Cell Force Spectroscopy
    Article Snippet: .. Calcein Staining of L929 Cells The live cell stain was stored as 1 mM Calcein AM (C3100MP, Life Technology) in DMSO(C-3099, Life Technology). .. For live staining of cells, a dilute 2 μM (1:500) solution of Calcein AM was prepared in PBS.

    Article Title: Biological and Pro-Angiogenic Properties of Genetically Modified Human Primary Myoblasts Overexpressing Placental Growth Factor in In Vitro and In Vivo Studies
    Article Snippet: .. Afterwards, the newly formed capillaries were stained with 2 μg/ml calcein-AM (Invitrogen, Carlsbad, USA). .. Myogenic Gene Expression Profiling To assess the myogenic gene expression, real-time PCR was performed.

    Article Title: Bifidobacterium dentium Fortifies the Intestinal Mucus Layer via Autophagy and Calcium Signaling Pathways
    Article Snippet: .. Following the experiment, cell viability was examined by staining cells with 3 μM calcein AM (live-cell dye, catalog no. C3099; Invitrogen) and 2.5 μM propidium iodide (dead-cell dye, catalog no. P4170; Sigma-Aldrich). ..

    Article Title: Infused polymers for cell sheet release
    Article Snippet: .. Transferred cells were washed with HBSS twice and then incubated in 2uM Calcein AM (Molecular Probes, C3099) and 2uM Sytox Orange (Molecular Probes, S11368) in Serum-free DMEM at 37 °C for 1 h. The stained cells were then washed twice with HBSS and imaged in Live Cell Imaging Buffer (Molecular Probes, A1429DJ). .. Cells growing on infused PDMS (state of cells before detachment) and cells growing on TCPS were also stained and imaged as controls.

    Article Title: PSMA-homing dsRNA chimeric protein vector kills prostate cancer cells and activates anti-tumor bystander responses
    Article Snippet: .. On Day 15, spheroids were stained with calcein AM (1:1000, c3099, Molecular Probes, Eugene, OR, USA) and 0.5μg/ml propidium iodide. .. Spheroids were monitored using confocal microscopy and size was measured using ImageJ software.

    Article Title: Mechanisms of Mycobacterium avium-induced resistance against insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice: role of Fas and Th1 cells
    Article Snippet: .. Viable target (T) lymphocytes were stained with Calcein-AM (CAM; Molecular Probes, Eugene, OR) 100 n m (30 min incubation at 37°C). .. E and T cells were cultured together in RPMI 1640 medium (G ibco BRL Life Technologies Ltd) containing 10% FCS, 200 m m l -glutamine, 10 m m HEPES (all from G ibco BRL) and 50 μ m 2-mercaptoethanol (2-ME; Sigma), for 3 h (37°C, 5% CO2 ).

    Chick Chorioallantoic Membrane Assay:

    Article Title: Mechanisms of Mycobacterium avium-induced resistance against insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice: role of Fas and Th1 cells
    Article Snippet: .. Viable target (T) lymphocytes were stained with Calcein-AM (CAM; Molecular Probes, Eugene, OR) 100 n m (30 min incubation at 37°C). .. E and T cells were cultured together in RPMI 1640 medium (G ibco BRL Life Technologies Ltd) containing 10% FCS, 200 m m l -glutamine, 10 m m HEPES (all from G ibco BRL) and 50 μ m 2-mercaptoethanol (2-ME; Sigma), for 3 h (37°C, 5% CO2 ).

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  • 90
    Thermo Fisher calcein am
    Calcein Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    calcein am - by Bioz Stars, 2020-01
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