Journal: PLoS ONE

Article Title: Effect of Sodium-Glucose Co-Transporter 2 Inhibitor, Dapagliflozin, on Renal Renin-Angiotensin System in an Animal Model of Type 2 Diabetes

doi: 10.1371/journal.pone.0165703

Figure Lengend Snippet: (A) Representative immunoblots of AT1R, Cu/ZnSOD, MnSOD and catalase. Quantitative analyses of the expression of AT1R (B), Cu/ZnSOD (C), MnSOD (D), catalase (E) in LETO (LT) and OLETF rats with saline (OL-C), dapagliflozine (OL-DA) or voglibose (OL-VO) treatment. * P < 0.05, OL-C vs. other groups; † P < 0.05, LT vs. other groups; ‡ P < 0.05, OL-DA vs. OL-VO. Values are expressed as means ± SE. AT1R, Ang II type I receptor

Article Snippet: For immunodetection, the blots were incubated overnight at 4°C in PBS containing 0.1% Tween-20 and 5% skim milk with primary antibodies raised against the following proteins: AT1R (Santa Cruz Biotechnology, Santa Cruz, CA), Copper/zinc superoxide dismutase (Cu/ZnSOD) (Enzo Life Sciences, Inc., NY), Manganese-superoxide dismutase (MnSOD), catalase, type IV collagen, and SGLT2 (all from Abcam); β-actin (Sigma-Aldrich); The second antibody was HRP-linked anti-Rabbit IgG (Cell Signaling Technology, Beverly, MA) for AT1R, Cu/ZnSOD, SGLT2, and HPR-linked anti-Mouse IgG (Cell Signaling Technology) for MnSOD and β-actin.

Techniques: Western Blot, Expressing

Journal: Oncotarget

Article Title: MicroRNA-153/Nrf-2/GPx1 pathway regulates radiosensitivity and stemness of glioma stem cells via reactive oxygen species

doi:

Figure Lengend Snippet: A. Detection of protein expression of catalase, MnSOD and GPx1 by Western blot. B. Enzymatic activities of catalase, MnSOD and GPx1 in GSCs and non-GSCs glioma cells. * P < 0.01 vs glioma cells. C. Detection of protein expression of GPx1 in GSCs transfected with GPx1-siRNA by Western blot. D. Flow cytometric analysis of ROS formation using the H2DCFH-DA probe in GSCs transfected with GPx1-siRNA. * P < 0.01 vs U87s/nc-siRNA or SU-2/nc-siRNA. # P < 0.01 vs U87s/GPx1-siRNA or SU-2/GPx1-siRNA. E. Survival curves of GSCs transfected with GPx1-siRNA. After exposure to 0, 2, 4, 6, 8 and 10 Gy X-ray irradiation, cell survival fractions were examined and the survival curves of cells were obtained from data fitting according to the linear quadratic model. Error bars indicate the standard error of the mean of three individual experiments. * P < 0.01 vs U87s or SU-2. D 0 (mean lethal dose) was calculated by the linear quadratic model.

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-Catalase, Manganese Superoxide Dismutase (MnSOD), Copper/Zinc Superoxide Dismutase (Cu/ZnSOD), GPx1, Nrf-2, Keap1, phospho-p38, p38, phospho-Activating transcription factor-2 (ATF-2), ATF-2, B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi1), Forkhead box O3 (FoxO3), Lamin b1 and β-actin (Santa Cruz Inc. California, USA).

Techniques: Expressing, Western Blot, Transfection, Irradiation