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Journal: bioRxiv

Article Title: Identification and optimization of trans-species reactive TfR1-binding VHH as tools for drug delivery across the blood-brain barrier

doi: 10.1101/2025.05.23.649291

Figure Lengend Snippet:

Article Snippet: Cloning of mouse, human and rhesus monkey TfR1 ectodomains in fusion with a mouse IgG2a Fc cDNAs encoding the mouse, human and rhesus monkey TfR1 ectodomains (amino acids 89 to 763 from mouse TfR1 (NP_001344227.1), amino acids 89 to 760 of the human TfR1 (AAA61153.1) and rhesus monkey TfR1 (NP_001244232.1)) were obtained by gene synthesis (Genecust) and cloned in the pINFUSE-mIgG2b-Fc2 vector (InvivoGen) to encode a mouse IgG2a Fc (mFc) fused in C-terminus to the mouse, human and rhesus monkey TfR1 ectodomains.

Techniques: Binding Assay, Recombinant