conical bottom 96 well plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher conical bottom 96 well plates
    Conical Bottom 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/conical bottom 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    conical bottom 96 well plates - by Bioz Stars, 2020-04
    99/100 stars

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    Centrifugation:

    Article Title: Identification of Genes Required for Secretion of the Francisella Oxidative Burst-Inhibiting Acid Phosphatase AcpA
    Article Snippet: The conditions included three different centrifugation schemes: (1) 16,100 × g once for 30 min in 1.5 ml microfuge tubes, (2) in a 96-well format, 2095 × g once for 40 min, and (3) in a 96-well format, 2095 × g for 40 min with the transfer of supernatants to another plate and centrifugation at 2095 × g for an additional 40 min. .. A volume of 50 μl of supernatant was dried in a 96-well immune-plate (Thermo Scientific, Rochester, NY, USA) overnight in the presence of protease inhibitor and then used for AcpA ELISA analysis as described below.

    Positive Control:

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For each plate, columns 1 and 12 were dedicated to controls: culture wells were alternatively spiked with 1 µl of DMSO alone (negative control) or supplemented with recombinant IFN-β so that final concentration equals 1,000 IU/ml (positive control). .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    Synthesized:

    Article Title: Protein Kinase C Regulates the Cell Surface Activity of Endothelin-Converting Enzyme-1
    Article Snippet: Cell-based Quenched Fluorescent Substrate Assay EA.hy926 cells grown in 96-well microtiter plates were washed with Opti-MEM (Invitrogen Australia, Mt. .. Following pre-incubation at 37°C for 30 min, a bradykinin-based quenched fluorescent substrate (QFS), (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(2, 4-dinitrophenyl) (custom synthesized by Auspep; Johnson and Ahn, ), was added to a final concentration of 40 μM.

    Blocking Assay:

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria. .. All plates were blocked with the blocking buffer (3% fatty acid free BSA, 20 mM Tris, 140 mM sodium chloride, 5 mM calcium chloride, pH 7.4) for 1 h at room temperature (RT) under humid conditions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Identification of Genes Required for Secretion of the Francisella Oxidative Burst-Inhibiting Acid Phosphatase AcpA
    Article Snippet: .. A volume of 50 μl of supernatant was dried in a 96-well immune-plate (Thermo Scientific, Rochester, NY, USA) overnight in the presence of protease inhibitor and then used for AcpA ELISA analysis as described below. .. The bacterial membrane protein FopA was included as a negative control.

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: Paragraph title: ELISA ... Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria.

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium. .. After 5 d in culture, the concentration of 1:20 diluted supernatant IFN-γ was quantified using IFN-γ ELISA kits (BD Biosciences).

    Article Title: Generation and characterization of monoclonal antibodies against the N-terminus of alpha-2-antiplasmin
    Article Snippet: Paragraph title: Characterization of selected mAbs directed against Met-α2AP by one-site non-competitive ELISA ... 96-well microtiter plates (Nunc A/S, Roskilde, Denmark) were coated with 2 μg/ml (200 μl per well in PBS containing 137 mM NaCl, 2.7 mM KCL, 6.5 mM Na2 HPO4 and 1 mM KH2 PO4 (pH 7.4)) of recombinant Met(R6)-α2AP, Met(W6)-α2AP or Asn-α2AP for 24 hours at 4°C.

    Incubation:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity. .. After 4 h, Gibco Dulbecco's modified Eagle's medium (Thermo Fisher Scientific, Inc.), supplemented with 10% equine serum, was removed from the plates, and the cell cultures were incubated with 2.0% B-27 neurobasal medium for 4 days.

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen). .. PEI and plasmids were mixed together and incubated for 30 min at room temperature.

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer
    Article Snippet: Colorectal and breast cancer cells (5 × 105 cells/ml) were plated in 24-well plates and incubated in a non-CO2 incubator for 18 h. The cells were then treated with Nar (3 mM) for 24 h. mRNA was extracted using the nucleospin RNAII ready-to-use system (MACHEREY-NAGEL), and 200 ng/μl of mRNA was used in the RT reaction. .. For each sample, 2.5 μl of cDNA and 12.5 μl of Taqman Universal Master Mix (2×) were used, and the final volume was adjusted to 25 μl with nuclease-free water on an optical 96-well reaction plate (Applied Biosystems).

    Article Title: Tumor cells and their crosstalk with endothelial cells in 3D spheroids
    Article Snippet: .. Spheroids formation using multi-well agarose-coated plates Agarose hydrogel 1.5% (100 µl) was added to each well of a 96-well culture plate (Thermo Fisher Scientific, Denmark), and incubated at 37 °C for 2 hours. .. A375, BxPC3, PANC1 and MDA-MB-231cells were seeded in 100 µl growth medium at a concentration of 5,000 cells per well.

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: Antifungal Activity Determination The MIC of all protein and peptide variants was determined on C. albicans with broth microdilution assays in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. In each well, 103 cells were incubated with increasing concentrations of antifungal proteins or peptides in a final volume of 200 μL 0.05× PDB.

    Article Title: Generation and characterization of monoclonal antibodies against the N-terminus of alpha-2-antiplasmin
    Article Snippet: 96-well microtiter plates (Nunc A/S, Roskilde, Denmark) were coated with 2 μg/ml (200 μl per well in PBS containing 137 mM NaCl, 2.7 mM KCL, 6.5 mM Na2 HPO4 and 1 mM KH2 PO4 (pH 7.4)) of recombinant Met(R6)-α2AP, Met(W6)-α2AP or Asn-α2AP for 24 hours at 4°C. .. Non-specific sites were blocked with 200 μl 1% bovine serum albumin (Sigma, Steinheim, Germany) in PBS and incubated for two hours at RT.

    Activity Assay:

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: .. The antifungal activity against C. albicans biofilms was determined in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. Cells (105 ) in 100 μL 0.05× PDB were distributed per well and plates were incubated at 37°C for up to 24 h to allow biofilm formation.

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: .. Antifungal Activity Determination The MIC of all protein and peptide variants was determined on C. albicans with broth microdilution assays in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. From a fresh C. albicans overnight culture on YPD agar, cells were harvested with an inoculation loop and suspended in 0.05× PDB (Sigma-Aldrich) ( ).

    Expressing:

    Article Title: Identification of Genes Required for Secretion of the Francisella Oxidative Burst-Inhibiting Acid Phosphatase AcpA
    Article Snippet: Both WT bacteria and the WT strain carrying the acpA expression plasmid (pacpA -Flag) were tested. .. A volume of 50 μl of supernatant was dried in a 96-well immune-plate (Thermo Scientific, Rochester, NY, USA) overnight in the presence of protease inhibitor and then used for AcpA ELISA analysis as described below.

    Article Title: Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma
    Article Snippet: .. Gene expression quantification was performed using TaqMan Array Human CYP450 and other Oxygenases 96-well plate (Applied Biosystems) by real time qPCR. ..

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer
    Article Snippet: For the comparative Ct method, we performed a two-step RT-PCR to obtain cDNA and real-time quantification using the target gene expression assays and Taqman Universal Master Mix (Applied Biosystems). .. For each sample, 2.5 μl of cDNA and 12.5 μl of Taqman Universal Master Mix (2×) were used, and the final volume was adjusted to 25 μl with nuclease-free water on an optical 96-well reaction plate (Applied Biosystems).

    Modification:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: The sections were then placed into a 0.25% pancreatic enzyme EDTA solution at 37°C for 20 min, followed by termination of digestion by addition of 5 ml Dulbecco's Modified Eagle medium (DMEM; Gibco, Thermo Fisher Scientific, Inc.) supplemented with 10% horse serum and 10% fetal bovine serum for 5 min. .. Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity.

    Real-time Polymerase Chain Reaction:

    Article Title: Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma
    Article Snippet: .. Gene expression quantification was performed using TaqMan Array Human CYP450 and other Oxygenases 96-well plate (Applied Biosystems) by real time qPCR. ..

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer
    Article Snippet: Paragraph title: Relative quantitative Real-Time PCR assay ... For each sample, 2.5 μl of cDNA and 12.5 μl of Taqman Universal Master Mix (2×) were used, and the final volume was adjusted to 25 μl with nuclease-free water on an optical 96-well reaction plate (Applied Biosystems).

    Transfection:

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: Cell transfection was performed with a 1 mg/ml poly-ethylenimine (PEI “Max”; Polyscience) solution adjusted at pH = 7. .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    Activation Assay:

    Article Title: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
    Article Snippet: Paragraph title: Allogeneic and anti-CD3/CD28–induced CD4+ T-cell activation ... Purified CD4+ T cells (105 /well) were cultured in 96-well flat-bottom plates with CD3/CD28 T-cell expansion Dynabeads (5 × 103 /well; Dynal ASA, Oslo, Norway).

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: Paragraph title: T-Cell Activation Assay. ... Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium.

    Protease Inhibitor:

    Article Title: Identification of Genes Required for Secretion of the Francisella Oxidative Burst-Inhibiting Acid Phosphatase AcpA
    Article Snippet: .. A volume of 50 μl of supernatant was dried in a 96-well immune-plate (Thermo Scientific, Rochester, NY, USA) overnight in the presence of protease inhibitor and then used for AcpA ELISA analysis as described below. .. The bacterial membrane protein FopA was included as a negative control.

    Transferring:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: The cells were isolated from the sections by a mechanical method using a Pasteur pipette, and passed through a 200 mesh stainless steel sieve. .. Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity.

    Cell Culture:

    Article Title: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
    Article Snippet: .. Purified CD4+ T cells (105 /well) were cultured in 96-well flat-bottom plates with CD3/CD28 T-cell expansion Dynabeads (5 × 103 /well; Dynal ASA, Oslo, Norway). ..

    Generated:

    Article Title: Protein Kinase C Regulates the Cell Surface Activity of Endothelin-Converting Enzyme-1
    Article Snippet: Cell-based Quenched Fluorescent Substrate Assay EA.hy926 cells grown in 96-well microtiter plates were washed with Opti-MEM (Invitrogen Australia, Mt. .. Fluorescence generated by cleavage of the QFS was continuously monitored (λ ex = 320 nm, λ em = 405 nm) in a FLUOStar Optima plate reader (BMG LabTech, Offenburg, Germany) maintained at 37°C.

    Imaging:

    Article Title: Plaque2.0—A High-Throughput Analysis Framework to Score Virus-Cell Transmission and Clonal Cell Expansion
    Article Snippet: .. All live imaging experiments were performed in 96-well black plates (Matrix; Thermo Fisher Scientific, Lausanne, Switzerland) and the entire wells were acquired in four sites by a motorized stage, allowing precise, high-speed selection of overlapping tiles. .. Confocal microscopy Confocal fluorescence microscopy was conducted with a Leica SP5 confocal laser scanning microscope (Leica Microsystems, Germany) equipped with a 63x objective (oil immersion; NA 1.4), a UV laser (355 nm), an argon laser (488 nm), and a diode laser (561 nm).

    Protein Concentration:

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: The MIC was defined as the protein concentration that inhibited growth by ≥90% compared to the untreated control, which was referred as 100% growth. .. The antifungal activity against C. albicans biofilms was determined in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer
    Article Snippet: For the comparative Ct method, we performed a two-step RT-PCR to obtain cDNA and real-time quantification using the target gene expression assays and Taqman Universal Master Mix (Applied Biosystems). .. For each sample, 2.5 μl of cDNA and 12.5 μl of Taqman Universal Master Mix (2×) were used, and the final volume was adjusted to 25 μl with nuclease-free water on an optical 96-well reaction plate (Applied Biosystems).

    Recombinant:

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For each plate, columns 1 and 12 were dedicated to controls: culture wells were alternatively spiked with 1 µl of DMSO alone (negative control) or supplemented with recombinant IFN-β so that final concentration equals 1,000 IU/ml (positive control). .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    Article Title: Generation and characterization of monoclonal antibodies against the N-terminus of alpha-2-antiplasmin
    Article Snippet: .. 96-well microtiter plates (Nunc A/S, Roskilde, Denmark) were coated with 2 μg/ml (200 μl per well in PBS containing 137 mM NaCl, 2.7 mM KCL, 6.5 mM Na2 HPO4 and 1 mM KH2 PO4 (pH 7.4)) of recombinant Met(R6)-α2AP, Met(W6)-α2AP or Asn-α2AP for 24 hours at 4°C. .. Non-specific sites were blocked with 200 μl 1% bovine serum albumin (Sigma, Steinheim, Germany) in PBS and incubated for two hours at RT.

    MTT Assay:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity. .. Subsequently, the cells were incubated with RDPS (0.025, 0.05, 0.10, 0.25, 0.50, 1.0, 2.0, 4.0 or 8.0 g/l) for 48 h, after which the culture medium (0.1 ml) was removed and added to 96-well plates containing 0.5% MTT for 4 h. Following removal of the culture media, 0.15 ml dimethyl sulfoxide (DMSO; Sigma-Aldrich) was added to the wells, and the plates were agitated for 10 min. Absorbance [optical density (OD)] values were measured at 490 nm using a microplate reader (EL×800™; BioTek Instruments, Inc., Winooski, VT, USA).

    Fluorescence:

    Article Title: Protein Kinase C Regulates the Cell Surface Activity of Endothelin-Converting Enzyme-1
    Article Snippet: Cell-based Quenched Fluorescent Substrate Assay EA.hy926 cells grown in 96-well microtiter plates were washed with Opti-MEM (Invitrogen Australia, Mt. .. Fluorescence generated by cleavage of the QFS was continuously monitored (λ ex = 320 nm, λ em = 405 nm) in a FLUOStar Optima plate reader (BMG LabTech, Offenburg, Germany) maintained at 37°C.

    Multiple Displacement Amplification:

    Article Title: Tumor cells and their crosstalk with endothelial cells in 3D spheroids
    Article Snippet: Spheroids formation using multi-well agarose-coated plates Agarose hydrogel 1.5% (100 µl) was added to each well of a 96-well culture plate (Thermo Fisher Scientific, Denmark), and incubated at 37 °C for 2 hours. .. A375, BxPC3, PANC1 and MDA-MB-231cells were seeded in 100 µl growth medium at a concentration of 5,000 cells per well.

    Isolation:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: The cells were isolated from the sections by a mechanical method using a Pasteur pipette, and passed through a 200 mesh stainless steel sieve. .. Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity.

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: .. Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium. .. Peptides used were the immunodominant H-2Kb –restricted MHC class I OVA-derived peptide SIINFEKL (InvivoGen) or the H-2 I-Ab MHC class II-restricted peptide ISQAVHAAHAEINEAGR (InvivoGen).

    Detection Assay:

    Article Title: Sulforaphane induces apoptosis in rhabdomyosarcoma and restores TRAIL-sensitivity in the aggressive alveolar subtype leading to tumor elimination in mice
    Article Snippet: Cell viability was determined using the Luminescence ATP Detection Assay System (ATPlite) (PerkinElmer). .. Cells were plated in triplicate using 96-well cluster plates: 20 × 103 CCA cells were plated with 100 μL of OPTI-MEM (GIBCO BRL) containing 4% FCS, 2 mM l -glutamine, and 1% penicillin/streptomycin.

    Microscopy:

    Article Title: Plaque2.0—A High-Throughput Analysis Framework to Score Virus-Cell Transmission and Clonal Cell Expansion
    Article Snippet: Paragraph title: Time-lapse multi-site, multi-channel microscopy ... All live imaging experiments were performed in 96-well black plates (Matrix; Thermo Fisher Scientific, Lausanne, Switzerland) and the entire wells were acquired in four sites by a motorized stage, allowing precise, high-speed selection of overlapping tiles.

    Purification:

    Article Title: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
    Article Snippet: .. Purified CD4+ T cells (105 /well) were cultured in 96-well flat-bottom plates with CD3/CD28 T-cell expansion Dynabeads (5 × 103 /well; Dynal ASA, Oslo, Norway). ..

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria. .. Alternatively, 50 μL containing 0.1 μg/μL of purified mycobacterial cell wall components were dried in ethanol onto duplicate or triplicate wells of a 96-well microtiter plate (EIA/RIA, CoStar, Corning, NY).

    Article Title: Generation and characterization of monoclonal antibodies against the N-terminus of alpha-2-antiplasmin
    Article Snippet: Purified mAbs were tested for their specific reactivities with α2AP by one-site non-competitive ELISA. .. 96-well microtiter plates (Nunc A/S, Roskilde, Denmark) were coated with 2 μg/ml (200 μl per well in PBS containing 137 mM NaCl, 2.7 mM KCL, 6.5 mM Na2 HPO4 and 1 mM KH2 PO4 (pH 7.4)) of recombinant Met(R6)-α2AP, Met(W6)-α2AP or Asn-α2AP for 24 hours at 4°C.

    Selection:

    Article Title: Plaque2.0—A High-Throughput Analysis Framework to Score Virus-Cell Transmission and Clonal Cell Expansion
    Article Snippet: .. All live imaging experiments were performed in 96-well black plates (Matrix; Thermo Fisher Scientific, Lausanne, Switzerland) and the entire wells were acquired in four sites by a motorized stage, allowing precise, high-speed selection of overlapping tiles. .. Confocal microscopy Confocal fluorescence microscopy was conducted with a Leica SP5 confocal laser scanning microscope (Leica Microsystems, Germany) equipped with a 63x objective (oil immersion; NA 1.4), a UV laser (355 nm), an argon laser (488 nm), and a diode laser (561 nm).

    Mouse Assay:

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: .. Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium. .. Peptides used were the immunodominant H-2Kb –restricted MHC class I OVA-derived peptide SIINFEKL (InvivoGen) or the H-2 I-Ab MHC class II-restricted peptide ISQAVHAAHAEINEAGR (InvivoGen).

    Viability Assay:

    Article Title: Sulforaphane induces apoptosis in rhabdomyosarcoma and restores TRAIL-sensitivity in the aggressive alveolar subtype leading to tumor elimination in mice
    Article Snippet: Paragraph title: Viability assay ... Cells were plated in triplicate using 96-well cluster plates: 20 × 103 CCA cells were plated with 100 μL of OPTI-MEM (GIBCO BRL) containing 4% FCS, 2 mM l -glutamine, and 1% penicillin/streptomycin.

    Plasmid Preparation:

    Article Title: Identification of Genes Required for Secretion of the Francisella Oxidative Burst-Inhibiting Acid Phosphatase AcpA
    Article Snippet: Both WT bacteria and the WT strain carrying the acpA expression plasmid (pacpA -Flag) were tested. .. A volume of 50 μl of supernatant was dried in a 96-well immune-plate (Thermo Scientific, Rochester, NY, USA) overnight in the presence of protease inhibitor and then used for AcpA ELISA analysis as described below.

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen). .. In parallel, 53 µg of poly(ethyleneimine) from Sigma-Aldrich (PEI) were diluted in 500 µl of DMEM (Gibco-BRL).

    Software:

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer
    Article Snippet: For each sample, 2.5 μl of cDNA and 12.5 μl of Taqman Universal Master Mix (2×) were used, and the final volume was adjusted to 25 μl with nuclease-free water on an optical 96-well reaction plate (Applied Biosystems). .. Real-time PCR was performed on an ABI 7000 SDS system using ABI Prism's SDS collection software version 1.1 (Applied Biosystems).

    Irradiation:

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: .. Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria. .. To confirm that NCRD binding was independent of the UV exposure, ELISA experiments were also performed without UV treatment with no significant differences seen in the results (data not shown).

    Negative Control:

    Article Title: Identification of Genes Required for Secretion of the Francisella Oxidative Burst-Inhibiting Acid Phosphatase AcpA
    Article Snippet: A volume of 50 μl of supernatant was dried in a 96-well immune-plate (Thermo Scientific, Rochester, NY, USA) overnight in the presence of protease inhibitor and then used for AcpA ELISA analysis as described below. .. The bacterial membrane protein FopA was included as a negative control.

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For each plate, columns 1 and 12 were dedicated to controls: culture wells were alternatively spiked with 1 µl of DMSO alone (negative control) or supplemented with recombinant IFN-β so that final concentration equals 1,000 IU/ml (positive control). .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    Binding Assay:

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: Binding of SP-D NCRD mutants to mycobacteria and/or their cell envelope components was performed by ELISA (Schlesinger et al. ). .. Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria.

    Concentration Assay:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: Next, the cells were counted and adjusted to a concentration of 5×106 /ml using DMEM. .. Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity.

    Article Title: Protein Kinase C Regulates the Cell Surface Activity of Endothelin-Converting Enzyme-1
    Article Snippet: Cell-based Quenched Fluorescent Substrate Assay EA.hy926 cells grown in 96-well microtiter plates were washed with Opti-MEM (Invitrogen Australia, Mt. .. Following pre-incubation at 37°C for 30 min, a bradykinin-based quenched fluorescent substrate (QFS), (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(2, 4-dinitrophenyl) (custom synthesized by Auspep; Johnson and Ahn, ), was added to a final concentration of 40 μM.

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For each plate, columns 1 and 12 were dedicated to controls: culture wells were alternatively spiked with 1 µl of DMSO alone (negative control) or supplemented with recombinant IFN-β so that final concentration equals 1,000 IU/ml (positive control). .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium. .. After 5 d in culture, the concentration of 1:20 diluted supernatant IFN-γ was quantified using IFN-γ ELISA kits (BD Biosciences).

    Article Title: Tumor cells and their crosstalk with endothelial cells in 3D spheroids
    Article Snippet: Spheroids formation using multi-well agarose-coated plates Agarose hydrogel 1.5% (100 µl) was added to each well of a 96-well culture plate (Thermo Fisher Scientific, Denmark), and incubated at 37 °C for 2 hours. .. A375, BxPC3, PANC1 and MDA-MB-231cells were seeded in 100 µl growth medium at a concentration of 5,000 cells per well.

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    Thermo Fisher nunc 96 well conical bottom plate
    Nunc 96 Well Conical Bottom Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher nunc 96 well polystyrene conical bottom microwell plates
    Nunc 96 Well Polystyrene Conical Bottom Microwell Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nunc 96 well polystyrene conical bottom microwell plates - by Bioz Stars, 2020-04
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