congo red staining deparaffinized (Millipore)

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Name:

Congo Red

Description:

Catalog Number:

c6767

Price:

None

Applications:

. Congo red has been used as a histochemical stain for the quantification of amyloid deposits in brain tissues. . It has been used as a cell wall inhibiting agent in fungus Cryptococcus neoformans. . It has also been used to study microstructural details in the cell walls of raw potato and fried potato using confocal microscopy.

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Millipore congo red staining deparaffinized

https://www.bioz.com/result/congo red staining deparaffinized/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

congo red staining deparaffinized - by Bioz Stars, 2021-03

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Incubation:

Article Title: Arginase 1 Insufficiency Precipitates Amyloid-β Deposition and Hastens Behavioral Impairment in a Mouse Model of Amyloidosis
Article Snippet: .. Briefly, brain sections were mounted and submerged for 20 min in alkaline sodium chloride, then incubated in 0.2% Congo Red solution (Sigma-Aldrich), Congo Red staining kit #HT60-1KT) for 30 min Sections were then rinsed in 95 and 100% ethanol, cleared in xylene for 15 min, and coverslipped with DPX mountant (Electron Microscopy Sciences). ..

Staining:

Article Title: Amyloid formation reduces protein kinase B phosphorylation in primary islet β-cells which is improved by blocking IL-1β signaling
Article Snippet: Also, all research protocols for human islets used in this study were approved by the Clinical Ethics Board of the University of British Columbia. .. Isolated human islets (purity ~90% as assessed by dithizone staining) were cultured at different conditions: free-floating in non-adherent culture plates in Ham’s/F10 (Invitrogen, Burlington, ON, CA) or CMRL (Mediatech, Herndon, VA, USA), with the amyloid-binding dye Congo red (25 μmol/L; Sigma-Aldrich, Oakville, ON, CA), exenatide (Byetta; 10nmol/L; Amylin Pharmaceuticals, San Diego, CA, USA) or anakinra (Kineret; 10μg/mL; Sobia Pharmaceutics, Denton, MD, USA). ..

Electron Microscopy:

Binding Assay:

Article Title: Oil Palm Phenolics Inhibit the In Vitro Aggregation of β-Amyloid Peptide into Oligomeric Complexes
Article Snippet: .. Congo Red Dye Binding Assay The Congo Red dye working solution was prepared as a 20 μ M Congo Red (Sigma Aldrich) in phosphate-buffered saline (PBS, pH 7.4). .. The Congo Red dye binding assays were performed with a TECAN Spectrafluor Plus instrument using 96-well plastic microtiter plates (BD Falcon).

Isolation:

Cell Culture:

Expressing:

Article Title: Real-time optotracing of curli and cellulose in live Salmonella biofilms using luminescent oligothiophenes
Article Snippet: .. Congo red assay Morphotypes based on curli and cellulose expression were observed after growth at 28 °C for 1–3 days on LB plates w/o salt, supplemented with Congo red (40 μg/ml, Sigma-Aldrich, Stockholm, Sweden) and Coomassie brilliant blue G-250 (20 μg/ml, Sigma) dissolved in 70% ethanol (Kemetyl AB, Haninge, Sweden). .. Inclined coverslip assay Exponentially growing cultures (37 °C, 230 r.p.m.) of indicated strains were harvested at OD600 =0.6, diluted to 105 CFU/ml in LB w/o salt, dispensed (8 ml aliquots) in six-well plates (Sarstedt, Numbrecht, Germany) with inclined glass coverslips (24×24 mm, VWR International, Stockholm, Sweden), and incubated for 48 h at 28 °C to allow biofilm formation.

other:

Article Title: Genome-Wide Analysis of Hypoxia-Responsive Genes in the Rice Blast Fungus, Magnaporthe oryzae
Article Snippet: Extracellular oxidative stress sensitivity test To determine the sensitivity to extracellular oxidative stress, mycelial growth rates were measured on solid CM supplemented H2 O2 (2.5 and 5 mM), 3 mM methyl viologen (paraquat, Aldrich, 856177) and 200 ppm Congo Red (Sigma, C6277).

Concentration Assay:

Article Title: Validation of Reference Genes for Robust qRT-PCR Gene Expression Analysis in the Rice Blast Fungus Magnaporthe oryzae
Article Snippet: .. For cell wall stress, the cell wall perturbant Congo Red (SIGMA-Aldrich) was prepared in a 1% stock solution, filter-sterilised and added to sterile Complete Medium buffered with 50 mM HEPES (pH 7.0) at a concentration of 100 μg/mL. .. Stress by caffeine was induced by adding 2.5 mM filter-sterilised caffeine (SIGMA-Aldrich) to sterile Complete Medium (pH 6.5).