concentrator 5  (Zymo Research)


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    Name:
    RNA Clean Concentrator 5
    Description:
    The RNA Clean Concentrator kits provide a simple and reliable method for the rapid preparation of high quality RT PCR ready DNA free R1013 R1014 RNA This simple procedure is based on the use of a unique single buffer system and Zymo Spin column technology that allows for selective recovery of total RNA 17 nt large RNAs 200 nt and or small RNAs 17 200 nt The procedure is easy Add binding buffer and ethanol to your sample then bind wash and elute ultra pure RNA The RNA can be eluted from the Zymo Spin IC Column in as little as 6 µl of RNase free water The highly concentrated purified RNA is suitable for all subsequent analyses and molecular manipulations
    Catalog Number:
    r1013
    Price:
    None
    Applications:
    RNA Purification
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research concentrator 5
    RNA Clean Concentrator 5
    The RNA Clean Concentrator kits provide a simple and reliable method for the rapid preparation of high quality RT PCR ready DNA free R1013 R1014 RNA This simple procedure is based on the use of a unique single buffer system and Zymo Spin column technology that allows for selective recovery of total RNA 17 nt large RNAs 200 nt and or small RNAs 17 200 nt The procedure is easy Add binding buffer and ethanol to your sample then bind wash and elute ultra pure RNA The RNA can be eluted from the Zymo Spin IC Column in as little as 6 µl of RNase free water The highly concentrated purified RNA is suitable for all subsequent analyses and molecular manipulations
    https://www.bioz.com/result/concentrator 5/product/Zymo Research
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    concentrator 5 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Selection:

    Article Title: Efficient depletion of ribosomal RNA for RNA sequencing in planarians
    Article Snippet: .. Use the RNA Clean µ Concentrator-5 kit (Zymo Research) to concentrate the ribodepleted samples, to carry out size selection and to digest any remaining DNA using DNase I treatment as described [ ]. ..

    RNA Sequencing Assay:

    Article Title: Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair
    Article Snippet: .. Following 3′ adapter ligation, excess adapter is removed by RecJ exonuclease (Epicenter, RJ411250) followed by purification of the RNA (RNA cleaning and concentration kit, Zymo, R1015) to further increase small RNA sequencing efficiency by reducing adapter-adapter ligations. .. Two biological repeats were sequenced on Illumina NextSeq500 1 × 75 bp high output mode generating 400 M single end reads.

    Ligation:

    Article Title: Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair
    Article Snippet: .. Following 3′ adapter ligation, excess adapter is removed by RecJ exonuclease (Epicenter, RJ411250) followed by purification of the RNA (RNA cleaning and concentration kit, Zymo, R1015) to further increase small RNA sequencing efficiency by reducing adapter-adapter ligations. .. Two biological repeats were sequenced on Illumina NextSeq500 1 × 75 bp high output mode generating 400 M single end reads.

    Isolation:

    Article Title: Mutant Best1 Expression and Impaired Phagocytosis in an iPSC Model of Autosomal Recessive Bestrophinopathy
    Article Snippet: .. Total RNA was isolated using RNA Clean and Concentrator-5 kit (Zymo Research, Irvine, California, USA). .. The yield of RNA was determined using a Nanodrop spectrophotometer.

    Transferring:

    Article Title: Mapping RNA Sequences that Contact Viral Capsid Proteins in Virions
    Article Snippet: .. Pipette tips (Corning, catalog number: 4154) 6-well clear polystyrene flat-bottomed tissue culture plate (Corning, Falcon® , catalog number: 351146) Polyallomer centrifuge tubes (Beckman Coulter, catalog number: 344060) Polypropylene microcentrifuge tubes and tips that are certified to be Ribonuclease free iBlot™ PVDF membrane (0.2 μm) (Thermo Fisher Scientific, Invitrogen™ , catalog number: IB401001) Razor blade (GEM, catalog number: RB-GEM-080014) 0.22 μm filter (EMD Millipore, catalog number: SCGPU05RE) Zymo RNA Clean & Concentrator (ZYMO RESEARCH, catalog number: R1015) BMV virions purified using cesium chloride density gradients and suspended in SAMA buffer Note: High purity BMV virions can be obtained as described in . ..

    Purification:

    Article Title: Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair
    Article Snippet: .. Following 3′ adapter ligation, excess adapter is removed by RecJ exonuclease (Epicenter, RJ411250) followed by purification of the RNA (RNA cleaning and concentration kit, Zymo, R1015) to further increase small RNA sequencing efficiency by reducing adapter-adapter ligations. .. Two biological repeats were sequenced on Illumina NextSeq500 1 × 75 bp high output mode generating 400 M single end reads.

    Article Title: CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
    Article Snippet: .. The reaction was then treated with DNase I to remove template DNA and RNA product was purified using an RNA Clean and Concentrator-5 (Zymo #R1016). .. Purified RNA was used as the template for reverse transcription using ProtoScript II according to manufacturer protocol, using sequence-specific primer RM511 for P(PGK1) and P(HIS4) plasmid libraries ( through ) or RM546 for P(Z) libraries ( and ).

    Article Title: Mapping RNA Sequences that Contact Viral Capsid Proteins in Virions
    Article Snippet: .. Pipette tips (Corning, catalog number: 4154) 6-well clear polystyrene flat-bottomed tissue culture plate (Corning, Falcon® , catalog number: 351146) Polyallomer centrifuge tubes (Beckman Coulter, catalog number: 344060) Polypropylene microcentrifuge tubes and tips that are certified to be Ribonuclease free iBlot™ PVDF membrane (0.2 μm) (Thermo Fisher Scientific, Invitrogen™ , catalog number: IB401001) Razor blade (GEM, catalog number: RB-GEM-080014) 0.22 μm filter (EMD Millipore, catalog number: SCGPU05RE) Zymo RNA Clean & Concentrator (ZYMO RESEARCH, catalog number: R1015) BMV virions purified using cesium chloride density gradients and suspended in SAMA buffer Note: High purity BMV virions can be obtained as described in . ..

    Concentration Assay:

    Article Title: Episo: quantitative estimation of RNA 5-methylcytosine at isoform level by high-throughput sequencing of RNA treated with bisulfite
    Article Snippet: .. The mRNA was cleaned using RNA clean kit (ZYMO REASEARCH R1015) and concentration was determined in nanodrop (Thermo Scientific, Nanodrop 1000) by measuring absorbance at 260 and 280 nm. .. Thirty microgram purified mRNA was mixed with 50 ng spike-in RNA.

    Article Title: Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair
    Article Snippet: .. Following 3′ adapter ligation, excess adapter is removed by RecJ exonuclease (Epicenter, RJ411250) followed by purification of the RNA (RNA cleaning and concentration kit, Zymo, R1015) to further increase small RNA sequencing efficiency by reducing adapter-adapter ligations. .. Two biological repeats were sequenced on Illumina NextSeq500 1 × 75 bp high output mode generating 400 M single end reads.

    other:

    Article Title: An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome
    Article Snippet: Zymo RNA Clean and Concentrator-5 kit (Zymo Research, Irvine, CA, USA).

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  • 99
    Zymo Research dna clean concentratortm 5 kit
    Dna Clean Concentratortm 5 Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna clean concentratortm 5 kit/product/Zymo Research
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dna clean concentratortm 5 kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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