concentrator 25 kit  (Zymo Research)


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  • 98
    Name:
    RNA Clean Concentrator 25
    Description:
    The RNA Clean Concentrator kits provide a simple and reliable method for the rapid preparation of high quality RT PCR ready DNA free R1013 R1014 RNA This simple procedure is based on the use of a unique single buffer system and Zymo Spin column technology that allows for selective recovery of total RNA 17 nt large RNAs 200 nt and or small RNAs 17 200 nt The procedure is easy Add binding buffer and ethanol to your sample then bind wash and elute ultra pure RNA The RNA can be eluted from the Zymo Spin IC Column in as little as 6 µl of RNase free water The highly concentrated purified RNA is suitable for all subsequent analyses and molecular manipulations
    Catalog Number:
    r1017
    Price:
    None
    Applications:
    RNA Purification
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research concentrator 25 kit
    The RNA Clean Concentrator kits provide a simple and reliable method for the rapid preparation of high quality RT PCR ready DNA free R1013 R1014 RNA This simple procedure is based on the use of a unique single buffer system and Zymo Spin column technology that allows for selective recovery of total RNA 17 nt large RNAs 200 nt and or small RNAs 17 200 nt The procedure is easy Add binding buffer and ethanol to your sample then bind wash and elute ultra pure RNA The RNA can be eluted from the Zymo Spin IC Column in as little as 6 µl of RNase free water The highly concentrated purified RNA is suitable for all subsequent analyses and molecular manipulations
    https://www.bioz.com/result/concentrator 25 kit/product/Zymo Research
    Average 98 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    concentrator 25 kit - by Bioz Stars, 2020-05
    98/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis
    Article Snippet: .. Vector (pcDNA3.1) cloning with Flag-tubulin substitution mutants (K40R) was conducted by the StarMut site-directed mutagenesis kit (GenStar, Cat#T111-01). mRNA was synthesized from linearized plasmid using the HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G(5′)ppp(5′)G (NEB), tailed with a poly(A) polymerase tailing kit (Epicentre), and purified with the RNA clean & concentrator-25 kit (Zymo Research). .. Kif18a siRNA injection Kif18a siRNA microinjection was used to knock down Kif18a in mouse oocytes.

    Synthesized:

    Article Title: Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis
    Article Snippet: .. Vector (pcDNA3.1) cloning with Flag-tubulin substitution mutants (K40R) was conducted by the StarMut site-directed mutagenesis kit (GenStar, Cat#T111-01). mRNA was synthesized from linearized plasmid using the HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G(5′)ppp(5′)G (NEB), tailed with a poly(A) polymerase tailing kit (Epicentre), and purified with the RNA clean & concentrator-25 kit (Zymo Research). .. Kif18a siRNA injection Kif18a siRNA microinjection was used to knock down Kif18a in mouse oocytes.

    Mutagenesis:

    Article Title: Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis
    Article Snippet: .. Vector (pcDNA3.1) cloning with Flag-tubulin substitution mutants (K40R) was conducted by the StarMut site-directed mutagenesis kit (GenStar, Cat#T111-01). mRNA was synthesized from linearized plasmid using the HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G(5′)ppp(5′)G (NEB), tailed with a poly(A) polymerase tailing kit (Epicentre), and purified with the RNA clean & concentrator-25 kit (Zymo Research). .. Kif18a siRNA injection Kif18a siRNA microinjection was used to knock down Kif18a in mouse oocytes.

    Isolation:

    Article Title: Macrophages attenuate the transcription of CYP1A1 in breast tumor cells and enhance their proliferation
    Article Snippet: .. Briefly, RNA was isolated out of 100 μl MCF7 tumor cell lysates using the RNA Clean and Concentrator-25 kit (Zymo Research). rRNA was removed using the RiboZero Gold rRNA Removal kit (Human/Mouse/Rat, Illumina). .. After heat fragmentation, end repair, and ligation of 3’ adapters, the RNA was reverse transcribed.

    Labeling:

    Article Title: Dissection of specific binding of HIV-1 Gag to the 'packaging signal' in viral RNA
    Article Snippet: .. To purify the RNAs the samples were run through three consecutive illustra microspin G-50 columns (GE Healthcare Life Sciences, Pittsburgh, PA USA) per 35 μL of labeling reaction, concentrated with an RNA clean and concentrator-25 kit (Zymo Research, Irvine, CA USA) and eluted with double-autoclaved double deionized water (dd-Water). .. The labeling yield was determined according to the manufacturer’s instructions with a UV-Vis NanoDrop 1000 and the integrity of the RNAs was determined by gel electrophoresis (6% polyacrylamide/TBE-UREA).

    Purification:

    Article Title: Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis
    Article Snippet: .. Vector (pcDNA3.1) cloning with Flag-tubulin substitution mutants (K40R) was conducted by the StarMut site-directed mutagenesis kit (GenStar, Cat#T111-01). mRNA was synthesized from linearized plasmid using the HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G(5′)ppp(5′)G (NEB), tailed with a poly(A) polymerase tailing kit (Epicentre), and purified with the RNA clean & concentrator-25 kit (Zymo Research). .. Kif18a siRNA injection Kif18a siRNA microinjection was used to knock down Kif18a in mouse oocytes.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis
    Article Snippet: .. Vector (pcDNA3.1) cloning with Flag-tubulin substitution mutants (K40R) was conducted by the StarMut site-directed mutagenesis kit (GenStar, CatT111-01). mRNA was synthesized from linearized plasmid using the HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G(5′)ppp(5′)G (NEB), tailed with a poly(A) polymerase tailing kit (Epicentre), and purified with the RNA clean & concentrator-25 kit (Zymo Research). .. Kif18a siRNA injection Kif18a siRNA microinjection was used to knock down Kif18a in mouse oocytes.

    Plasmid Preparation:

    Article Title: Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis
    Article Snippet: .. Vector (pcDNA3.1) cloning with Flag-tubulin substitution mutants (K40R) was conducted by the StarMut site-directed mutagenesis kit (GenStar, Cat#T111-01). mRNA was synthesized from linearized plasmid using the HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G(5′)ppp(5′)G (NEB), tailed with a poly(A) polymerase tailing kit (Epicentre), and purified with the RNA clean & concentrator-25 kit (Zymo Research). .. Kif18a siRNA injection Kif18a siRNA microinjection was used to knock down Kif18a in mouse oocytes.

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    Zymo Research concentratortm 25 kit
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    https://www.bioz.com/result/concentratorã cents ⠄ â cents 25 kit/product/Zymo Research
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