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Bangs Laboratories concanavalin a coated magnetic beads
An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome
Concanavalin A Coated Magnetic Beads, supplied by Bangs Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/concanavalin a coated magnetic beads/product/Bangs Laboratories
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
concanavalin a coated magnetic beads - by Bioz Stars, 2021-03
95/100 stars

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1) Product Images from "Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs"

Article Title: Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs

Journal: Epigenetics & Chromatin

doi: 10.1186/s13072-018-0243-8

An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome
Figure Legend Snippet: An automated platform for high-throughput in situ profiling of chromatin proteins. a AutoCUT RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. b Hierarchically clustered correlation matrix of AutoCUT RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome

Techniques Used: High Throughput Screening Assay, In Situ, Incubation, Blocking Assay, Transformation Assay

2) Product Images from "Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs"

Article Title: Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs

Journal: Epigenetics & Chromatin

doi: 10.1186/s13072-018-0243-8

An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome
Figure Legend Snippet: An automated platform for high-throughput in situ profiling of chromatin proteins. a AutoCUT RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. b Hierarchically clustered correlation matrix of AutoCUT RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome

Techniques Used: High Throughput Screening Assay, In Situ, Incubation, Blocking Assay, Transformation Assay

3) Product Images from "Automated in situ profiling of chromatin modifications resolves cell types and gene regulatory programs"

Article Title: Automated in situ profiling of chromatin modifications resolves cell types and gene regulatory programs

Journal: bioRxiv

doi: 10.1101/418681

An automated platform for high-throughput  in situ  profiling of chromatin proteins. ( a ) AutoCUT  RUN workflow. (1) Cells or tissue are bound to Concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. ( b ) Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2  transformed values of read counts split into 500 bp bins across the genome.
Figure Legend Snippet: An automated platform for high-throughput in situ profiling of chromatin proteins. ( a ) AutoCUT RUN workflow. (1) Cells or tissue are bound to Concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. ( b ) Hierarchically clustered correlation matrix of AutoCUT RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 transformed values of read counts split into 500 bp bins across the genome.

Techniques Used: High Throughput Screening Assay, In Situ, Incubation, Blocking Assay, Transformation Assay

Related Articles

Purification:

Article Title: Winding single-molecule double-stranded DNA on a nanometer-sized reel
Article Snippet: PCR products were purified using the Wizard PCR clean-up system (Promega, USA). .. Purified DNA (12.5 µg) was incubated with 100 µl of streptavidin-coated polystyrene beads (0.5 µm diameter, 1% wt/vol, Bangs Laboratories, USA) suspension overnight, and unbound DNA was removed by washing several times using buffer (100 mM KPi, pH 7.0). ..

Incubation:

Article Title: Winding single-molecule double-stranded DNA on a nanometer-sized reel
Article Snippet: PCR products were purified using the Wizard PCR clean-up system (Promega, USA). .. Purified DNA (12.5 µg) was incubated with 100 µl of streptavidin-coated polystyrene beads (0.5 µm diameter, 1% wt/vol, Bangs Laboratories, USA) suspension overnight, and unbound DNA was removed by washing several times using buffer (100 mM KPi, pH 7.0). ..

Article Title: High-Mobility Group Box 1-Induced Complement Activation Causes Sterile Inflammation
Article Snippet: Biotinylated human IgG was used as a positive control. .. Streptavidin-coated microspheres (Bangs Laboratories Inc.) were incubated with biotin-labeled HMGB1 for 1 h at RT. .. The HMGB1- or human IgG-coated microspheres were washed three times with GVB2+ buffer and incubated with 10% NHS for 30 min at 37°C.

Modification:

Article Title: Sensitive detection of nucleic acids by PNA hybridization directed co-localization of fluorescent beads
Article Snippet: .. The red fluorescent beads (RB) were magnetic (for capturing), streptavidin surface modified and 5.9 µm in size (QuantumPlexM SP Streptavidin (Bangs laboratories)). .. The green fluorescent (GB) were surface modified with avidin and 1.01 µm in size (Neutral avidin coated Dragon Green polystyrene beads (Bangs laboratories)).

Labeling:

Article Title: Unexpected sequences and structures of mtDNA required for efficient transcription from the first heavy-strand promoter
Article Snippet: .. A single phage-λ DNA molecule (48,500 base pairs) labeled on the termini of its opposite strands with biotin can be attached by its termini to streptavidin-coated polystyrene beads (Bangs Labs). ..

Staining:

Article Title: Key residues at the membrane-distal surface of KACL, but not glycosylation, determine the functional interaction of the keratinocyte-specific C-type lectin-like receptor KACL with its high-affinity receptor NKp65
Article Snippet: Ectodomains of NKp65 were biotinylated using BirA ligase and, before use, were tetramerized using APC-labelled or phycoerythrin-labelled streptavidin (Jackson ImmunoResearch). .. Streptavidin-coated microspheres (Bangs Laboratories, Inc., Indianapolis, IN) were loaded with 1 μg biotinylated ectodomains and stained as described above. .. Antibodies: anti-CD107a-APC (BD Biosciences), anti-FLAG (M2; Sigma-Aldrich), CD56-Peridinin chlorophyll protein (PerCP, Biolegend, San Diego, CA).

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    Bangs Laboratories concanavalin a coated magnetic beads
    An automated platform for high-throughput  in situ  profiling of chromatin proteins. ( a ) AutoCUT  RUN workflow. (1) Cells or tissue are bound to Concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. ( b ) Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2  transformed values of read counts split into 500 bp bins across the genome.
    Concanavalin A Coated Magnetic Beads, supplied by Bangs Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/concanavalin a coated magnetic beads/product/Bangs Laboratories
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    concanavalin a coated magnetic beads - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

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    An automated platform for high-throughput  in situ  profiling of chromatin proteins. ( a ) AutoCUT  RUN workflow. (1) Cells or tissue are bound to Concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. ( b ) Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2  transformed values of read counts split into 500 bp bins across the genome.

    Journal: bioRxiv

    Article Title: Automated in situ profiling of chromatin modifications resolves cell types and gene regulatory programs

    doi: 10.1101/418681

    Figure Lengend Snippet: An automated platform for high-throughput in situ profiling of chromatin proteins. ( a ) AutoCUT RUN workflow. (1) Cells or tissue are bound to Concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. ( b ) Hierarchically clustered correlation matrix of AutoCUT RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 transformed values of read counts split into 500 bp bins across the genome.

    Article Snippet: Concanavalin A-coated magnetic beads (Bangs Laboratories, ca. no. BP531).

    Techniques: High Throughput Screening Assay, In Situ, Incubation, Blocking Assay, Transformation Assay

    An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome

    Journal: Epigenetics & Chromatin

    Article Title: Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs

    doi: 10.1186/s13072-018-0243-8

    Figure Lengend Snippet: An automated platform for high-throughput in situ profiling of chromatin proteins. a AutoCUT RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. b Hierarchically clustered correlation matrix of AutoCUT RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome

    Article Snippet: Briefly, cells or tissue samples are bound to concanavalin A-coated magnetic beads (Bangs Laboratories, ca. no. BP531), permeabilized with digitonin, and bound with a protein specific antibody as previously described [ ].

    Techniques: High Throughput Screening Assay, In Situ, Incubation, Blocking Assay, Transformation Assay

    An automated platform for high-throughput in situ profiling of chromatin proteins.  a  AutoCUT  RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT  RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced.  b  Hierarchically clustered correlation matrix of AutoCUT  RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome

    Journal: Epigenetics & Chromatin

    Article Title: Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs

    doi: 10.1186/s13072-018-0243-8

    Figure Lengend Snippet: An automated platform for high-throughput in situ profiling of chromatin proteins. a AutoCUT RUN workflow. (1) Cells or tissue are bound to concanavalin A-coated beads, permeabilized with digitonin, and incubated with an antibody targeting a chromatin protein. (2) Samples are arrayed in a 96-well plate and (3) processed on a Biomek robot fitted with a 96-well magnetic plate for magnetic separation during washes (α), and an aluminum chiller block (β) routed to a circulating water bath (γ) for temperature control. (4) AutoCUT RUN produces in 2 days up to 96 libraries that are ready to be pooled and sequenced. b Hierarchically clustered correlation matrix of AutoCUT RUN profiles of histone-H3 modifications that mark active (pink) and repressed (blue) chromatin in H1 (orange) and K562 (purple) cells. Pearson correlations were calculated using the log 2 -transformed values of read counts split into 500 bp bins across the genome

    Article Snippet: Briefly, cells or tissue samples are bound to concanavalin A-coated magnetic beads (Bangs Laboratories, ca. no. BP531), permeabilized with digitonin, and bound with a protein specific antibody as previously described [ ].

    Techniques: High Throughput Screening Assay, In Situ, Incubation, Blocking Assay, Transformation Assay