Structured Review

Boehringer Mannheim complete protease inhibitor mix
Complete Protease Inhibitor Mix, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complete protease inhibitor mix/product/Boehringer Mannheim
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
complete protease inhibitor mix - by Bioz Stars, 2020-11
91/100 stars

Images

Related Articles

Transfection:

Article Title: Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3- 3-dependent cellular localization
Article Snippet: .. Forty-eight hours after transfection, cells were lysed in JLB (50 mM Tris⋅HCl, pH 8/150 mM NaCl/10% glycerol/0.5% TritonX-100) containing a complete protease inhibitor mixture (Boehringer Mannheim) and phosphatase inhibitors [20 mM NaH2 (PO4 ), pH 7.2/25 mM NaF/2 mM EDTA]. .. Lysis proceeded for 10 min at 4°C, after which the cellular debris was pelleted by centrifugation at 14,000 × g for 5 min. Recombinant proteins were immunoprecipitated from the supernatant by incubation with α-FLAG M2 agarose affinity gel (Sigma) for 1 h at 4°C.

Protease Inhibitor:

Article Title: Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3- 3-dependent cellular localization
Article Snippet: .. Forty-eight hours after transfection, cells were lysed in JLB (50 mM Tris⋅HCl, pH 8/150 mM NaCl/10% glycerol/0.5% TritonX-100) containing a complete protease inhibitor mixture (Boehringer Mannheim) and phosphatase inhibitors [20 mM NaH2 (PO4 ), pH 7.2/25 mM NaF/2 mM EDTA]. .. Lysis proceeded for 10 min at 4°C, after which the cellular debris was pelleted by centrifugation at 14,000 × g for 5 min. Recombinant proteins were immunoprecipitated from the supernatant by incubation with α-FLAG M2 agarose affinity gel (Sigma) for 1 h at 4°C.

Article Title: ExbBD-Dependent Transport of Maltodextrins through the Novel MalA Protein across the Outer Membrane of Caulobacter crescentus
Article Snippet: .. Cells were harvested by centrifugation for 20 min at 10,000 × g , washed with 50 mM Tris-HCl, pH 8, and suspended in 15 ml of 60 mM Tris-HCl, pH 8, 0.2 mM EDTA, 0.6 ml of Complete protease inhibitor mixture (Boehringer, Mannheim, Germany), 0.15 ml of 1 mM phenylmethylsulfonyl fluoride, and 0.3 ml of DNase (1 mg per ml). .. Cells were disrupted in a French pressure cell three times at 16,000 lb/in2 , and cell debris was pelleted by centrifugation twice at 10,000 × g for 20 min. Fifteen milliliters of extraction buffer (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2 , 2% Triton X-100) was added.

Article Title: Functional communication between endogenous BRCA1 and its partner, BARD1, during Xenopus laevis development
Article Snippet: .. Cells were lysed in a buffer containing 100 mM Hepes (pH 7.5), 200 mM NaCl, 40 mM EDTA, 4 mM EGTA, 100 mM NaF, 20 mM β-glycerophosphate, 2 mM sodium orthovanadate, 1% Nonidet P-40, and 1 tablet per 50 ml of the Complete Protease Inhibitor mixture (Boehringer Mannheim). .. For immunoprecipitations (IPs), extracts from 293 T cells or from 8–10 eggs or embryos were incubated with 1 μg of Ab on ice for 2–3 h, followed by incubation with 20 μl of protein A- or protein G-Sepharose beads (Amersham Pharmacia).

Article Title: Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels fromEscherichia coli
Article Snippet: .. Complete protease inhibitor mixture was from Boehringer Mannheim. .. Restriction and DNA modifying enzymes were from New England Biolabs or Life Technologies.

Article Title: CD5 Negatively Regulates the T-Cell Antigen Receptor Signal Transduction Pathway: Involvement of SH2-Containing Phosphotyrosine Phosphatase SHP-1
Article Snippet: .. After stimulation, cells were lysed in 1 ml of lysis buffer containing (50 mM Tris [pH 7.5], 1% NP-40, 150 mM NaCl, 2 mM EGTA, 1 mM sodium orthovanadate) plus Complete protease inhibitor mixture (Boehringer Mannheim). .. Samples were centrifuged at 14,000 rpm for 2 min (to remove nuclei); lysates were precleared twice with 50 μl of rat or mouse Ig-coupled CNBr-activated Sepharose beads (Pharmacia) or protein A-Sepharose (Pharmacia) for 60 min at 4°C and subjected to immunoprecipitation with antibody to mCD6 (M6-3E.124) covalently coupled to CNBr-activated Sepharose or with immune rabbit antisera to specific proteins.

Article Title: Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts
Article Snippet: .. Lysis buffers were supplemented with 1 mM of sodium vanadate and Complete™ protease inhibitor mixture ( Boehringer Mannheim GmbH ). .. For each immunoprecipitation, 500 μg of total cell lysate was incubated with 1 μg of antibody and 30 μl of protein G–Agarose ( GIBCO BRL ) to a total volume of 1 ml in their respective lysis buffer (supplemented with vanadate and protease inhibitors) at 4°C for 90 min.

Article Title: Potent histone deacetylase inhibitors built from trichostatin A and cyclic tetrapeptide antibiotics including trapoxin
Article Snippet: .. After the cleared supernatant had been incubated for 1 h at 4°C with 8 μg/ml of an anti-FLAG M2 antibody (Sigma) for HDAC1 and HDAC4, or an anti-HA antibody (Santa Cruz Biotechnology) for HDAC6, the agarose beads were washed three times with lysis buffer and once with HD buffer consisting of 20 mM Tris⋅HCl (pH 8.0), 150 mM NaCl, 10% glycerol, and a complete protease inhibitor mixture (Boehringer Mannheim). .. The bound proteins were released from the immune complex by incubating for 1 h at 4°C with 100 μg of the FLAG or the HA peptide (Sigma-Aldrich) in HD buffer (1.25 ml).

Article Title: CD5 Negatively Regulates the T-Cell Antigen Receptor Signal Transduction Pathway: Involvement of SH2-Containing Phosphotyrosine Phosphatase SHP-1
Article Snippet: .. Jurkat cells (8 × 107 ), unstimulated or stimulated with biotinylated anti-CD3 MAb G19-4 at 10 μg/ml plus avidin at 50 μg/ml for 5 min at 37°C, were lysed into 600 μl of lysis buffer (50 mM Tris HCl, 150 mM NaCl, 1% Nonidet P-40 [NP-40] [pH 7.5]) plus Complete protease inhibitor mixture (Boehringer Mannheim, Indianapolis, Ind.). .. Nuclei and unlysed cells were removed by centrifugation at 4°C for 10 min at 14,000 rpm.

Centrifugation:

Article Title: ExbBD-Dependent Transport of Maltodextrins through the Novel MalA Protein across the Outer Membrane of Caulobacter crescentus
Article Snippet: .. Cells were harvested by centrifugation for 20 min at 10,000 × g , washed with 50 mM Tris-HCl, pH 8, and suspended in 15 ml of 60 mM Tris-HCl, pH 8, 0.2 mM EDTA, 0.6 ml of Complete protease inhibitor mixture (Boehringer, Mannheim, Germany), 0.15 ml of 1 mM phenylmethylsulfonyl fluoride, and 0.3 ml of DNase (1 mg per ml). .. Cells were disrupted in a French pressure cell three times at 16,000 lb/in2 , and cell debris was pelleted by centrifugation twice at 10,000 × g for 20 min. Fifteen milliliters of extraction buffer (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2 , 2% Triton X-100) was added.

Avidin-Biotin Assay:

Article Title: CD5 Negatively Regulates the T-Cell Antigen Receptor Signal Transduction Pathway: Involvement of SH2-Containing Phosphotyrosine Phosphatase SHP-1
Article Snippet: .. Jurkat cells (8 × 107 ), unstimulated or stimulated with biotinylated anti-CD3 MAb G19-4 at 10 μg/ml plus avidin at 50 μg/ml for 5 min at 37°C, were lysed into 600 μl of lysis buffer (50 mM Tris HCl, 150 mM NaCl, 1% Nonidet P-40 [NP-40] [pH 7.5]) plus Complete protease inhibitor mixture (Boehringer Mannheim, Indianapolis, Ind.). .. Nuclei and unlysed cells were removed by centrifugation at 4°C for 10 min at 14,000 rpm.

Incubation:

Article Title: Potent histone deacetylase inhibitors built from trichostatin A and cyclic tetrapeptide antibiotics including trapoxin
Article Snippet: .. After the cleared supernatant had been incubated for 1 h at 4°C with 8 μg/ml of an anti-FLAG M2 antibody (Sigma) for HDAC1 and HDAC4, or an anti-HA antibody (Santa Cruz Biotechnology) for HDAC6, the agarose beads were washed three times with lysis buffer and once with HD buffer consisting of 20 mM Tris⋅HCl (pH 8.0), 150 mM NaCl, 10% glycerol, and a complete protease inhibitor mixture (Boehringer Mannheim). .. The bound proteins were released from the immune complex by incubating for 1 h at 4°C with 100 μg of the FLAG or the HA peptide (Sigma-Aldrich) in HD buffer (1.25 ml).

Lysis:

Article Title: CD5 Negatively Regulates the T-Cell Antigen Receptor Signal Transduction Pathway: Involvement of SH2-Containing Phosphotyrosine Phosphatase SHP-1
Article Snippet: .. After stimulation, cells were lysed in 1 ml of lysis buffer containing (50 mM Tris [pH 7.5], 1% NP-40, 150 mM NaCl, 2 mM EGTA, 1 mM sodium orthovanadate) plus Complete protease inhibitor mixture (Boehringer Mannheim). .. Samples were centrifuged at 14,000 rpm for 2 min (to remove nuclei); lysates were precleared twice with 50 μl of rat or mouse Ig-coupled CNBr-activated Sepharose beads (Pharmacia) or protein A-Sepharose (Pharmacia) for 60 min at 4°C and subjected to immunoprecipitation with antibody to mCD6 (M6-3E.124) covalently coupled to CNBr-activated Sepharose or with immune rabbit antisera to specific proteins.

Article Title: Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts
Article Snippet: .. Lysis buffers were supplemented with 1 mM of sodium vanadate and Complete™ protease inhibitor mixture ( Boehringer Mannheim GmbH ). .. For each immunoprecipitation, 500 μg of total cell lysate was incubated with 1 μg of antibody and 30 μl of protein G–Agarose ( GIBCO BRL ) to a total volume of 1 ml in their respective lysis buffer (supplemented with vanadate and protease inhibitors) at 4°C for 90 min.

Article Title: Potent histone deacetylase inhibitors built from trichostatin A and cyclic tetrapeptide antibiotics including trapoxin
Article Snippet: .. After the cleared supernatant had been incubated for 1 h at 4°C with 8 μg/ml of an anti-FLAG M2 antibody (Sigma) for HDAC1 and HDAC4, or an anti-HA antibody (Santa Cruz Biotechnology) for HDAC6, the agarose beads were washed three times with lysis buffer and once with HD buffer consisting of 20 mM Tris⋅HCl (pH 8.0), 150 mM NaCl, 10% glycerol, and a complete protease inhibitor mixture (Boehringer Mannheim). .. The bound proteins were released from the immune complex by incubating for 1 h at 4°C with 100 μg of the FLAG or the HA peptide (Sigma-Aldrich) in HD buffer (1.25 ml).

Article Title: CD5 Negatively Regulates the T-Cell Antigen Receptor Signal Transduction Pathway: Involvement of SH2-Containing Phosphotyrosine Phosphatase SHP-1
Article Snippet: .. Jurkat cells (8 × 107 ), unstimulated or stimulated with biotinylated anti-CD3 MAb G19-4 at 10 μg/ml plus avidin at 50 μg/ml for 5 min at 37°C, were lysed into 600 μl of lysis buffer (50 mM Tris HCl, 150 mM NaCl, 1% Nonidet P-40 [NP-40] [pH 7.5]) plus Complete protease inhibitor mixture (Boehringer Mannheim, Indianapolis, Ind.). .. Nuclei and unlysed cells were removed by centrifugation at 4°C for 10 min at 14,000 rpm.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Boehringer Mannheim complete protease inhibitor mixture
    Complete Protease Inhibitor Mixture, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete protease inhibitor mixture/product/Boehringer Mannheim
    Average 92 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    complete protease inhibitor mixture - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

    88
    Boehringer Mannheim mini complete protease inhibitor mixture
    Mini Complete Protease Inhibitor Mixture, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mini complete protease inhibitor mixture/product/Boehringer Mannheim
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mini complete protease inhibitor mixture - by Bioz Stars, 2020-11
    88/100 stars
      Buy from Supplier

    Image Search Results