pcr amplification  (TaKaRa)

 
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    Name:
    PCR Amplification Kit
    Description:
    The PCR Amplification Kit which includes TaKaRa Taq DNA Polymerase is designed to perform PCR on any DNA template and includes all of the reagents necessary for PCR including dNTP mixture two buffers with and without Mg2 and MgCl2 The kit comes with control template and primers for verifying amplification conditions DNA size markers and loading buffer
    Catalog Number:
    r011
    Price:
    None
    Size:
    100 Rxns
    Category:
    Takara Taq PCR kit Takara Taq products Standard PCR PCR
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    Structured Review

    TaKaRa pcr amplification
    <t>Nrf1b</t> expression is widely distributed. Nrf1a and Nrf1b mRNA expression patterns were analyzed by <t>RT-PCR</t> in various cell lines ( A ) and mouse tissues ( B ) . Nrf1a and Nrf1b cDNA was amplified by PCR for 30 cycles and 18S was amplified for 20 cycles. Histograms show relative Nrf1a and Nrf1b expression normalized against 18S. ( C ) . Western blot of different mouse tissues probed with Nrf1 antibody. HEK293 cells transfected with pEF1-Nrf1a (lane 1), and pEF1-Nrf1b (lane 2) were used as controls for detection of the Nrf1a and Nrf1b isoforms by the Nrf1 antibody. Beta-actin was used as a loading control.
    The PCR Amplification Kit which includes TaKaRa Taq DNA Polymerase is designed to perform PCR on any DNA template and includes all of the reagents necessary for PCR including dNTP mixture two buffers with and without Mg2 and MgCl2 The kit comes with control template and primers for verifying amplification conditions DNA size markers and loading buffer
    https://www.bioz.com/result/pcr amplification/product/TaKaRa
    Average 99 stars, based on 12675 article reviews
    Price from $9.99 to $1999.99
    pcr amplification - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Characterization of Nrf1b, a Novel Isoform of the Nuclear Factor-Erythroid-2 Related Transcription Factor-1 That Activates Antioxidant Response Element-Regulated Genes"

    Article Title: Characterization of Nrf1b, a Novel Isoform of the Nuclear Factor-Erythroid-2 Related Transcription Factor-1 That Activates Antioxidant Response Element-Regulated Genes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0048404

    Nrf1b expression is widely distributed. Nrf1a and Nrf1b mRNA expression patterns were analyzed by RT-PCR in various cell lines ( A ) and mouse tissues ( B ) . Nrf1a and Nrf1b cDNA was amplified by PCR for 30 cycles and 18S was amplified for 20 cycles. Histograms show relative Nrf1a and Nrf1b expression normalized against 18S. ( C ) . Western blot of different mouse tissues probed with Nrf1 antibody. HEK293 cells transfected with pEF1-Nrf1a (lane 1), and pEF1-Nrf1b (lane 2) were used as controls for detection of the Nrf1a and Nrf1b isoforms by the Nrf1 antibody. Beta-actin was used as a loading control.
    Figure Legend Snippet: Nrf1b expression is widely distributed. Nrf1a and Nrf1b mRNA expression patterns were analyzed by RT-PCR in various cell lines ( A ) and mouse tissues ( B ) . Nrf1a and Nrf1b cDNA was amplified by PCR for 30 cycles and 18S was amplified for 20 cycles. Histograms show relative Nrf1a and Nrf1b expression normalized against 18S. ( C ) . Western blot of different mouse tissues probed with Nrf1 antibody. HEK293 cells transfected with pEF1-Nrf1a (lane 1), and pEF1-Nrf1b (lane 2) were used as controls for detection of the Nrf1a and Nrf1b isoforms by the Nrf1 antibody. Beta-actin was used as a loading control.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Western Blot, Transfection

    2) Product Images from "Rapid and Efficient Co-Transcriptional Splicing Enhances Mammalian Gene Expression"

    Article Title: Rapid and Efficient Co-Transcriptional Splicing Enhances Mammalian Gene Expression

    Journal: bioRxiv

    doi: 10.1101/2020.02.11.944595

    Related to Figure 1. Splicing is not ongoing during chromatin purification and nascent RNA isolation (A) RT-PCR on total RNA collected from MEL cells treated with 1 μM splicing inhibitor Pladienolide B in cell culture for 0, 1, 2, and 4 hours. Total RNA was reverse transcribed with random hexamers, and PCR primers span a single intron in each gene. Three representative genes are shown (left: Brd2 , middle: Dnajb1 , and right: Riok3 ). M indicates mock treatment with DMSO, and G indicates amplification of genomic DNA to determine the size of unspliced RNA. U indicates size of unspliced amplicon, and S indicates size of spliced amplicon. (B) RT-qPCR from total RNA (as in (A) ), showing six additional genes ( Slc12a6 , Dmtn , Hnrnpll , Rbm39 , Hbq1b , C1qbp ) after treatment with 1 μM Pladienolide B for 0h and 4h. (C) RT-PCR on nascent RNA isolated from chromatin which was fractionated in the absence (-) or presence (+) of 1 μM Pladienolide B. Nascent RNA was reverse transcribed with random hexamers and PCR primers were the same as in (A) and (B) .
    Figure Legend Snippet: Related to Figure 1. Splicing is not ongoing during chromatin purification and nascent RNA isolation (A) RT-PCR on total RNA collected from MEL cells treated with 1 μM splicing inhibitor Pladienolide B in cell culture for 0, 1, 2, and 4 hours. Total RNA was reverse transcribed with random hexamers, and PCR primers span a single intron in each gene. Three representative genes are shown (left: Brd2 , middle: Dnajb1 , and right: Riok3 ). M indicates mock treatment with DMSO, and G indicates amplification of genomic DNA to determine the size of unspliced RNA. U indicates size of unspliced amplicon, and S indicates size of spliced amplicon. (B) RT-qPCR from total RNA (as in (A) ), showing six additional genes ( Slc12a6 , Dmtn , Hnrnpll , Rbm39 , Hbq1b , C1qbp ) after treatment with 1 μM Pladienolide B for 0h and 4h. (C) RT-PCR on nascent RNA isolated from chromatin which was fractionated in the absence (-) or presence (+) of 1 μM Pladienolide B. Nascent RNA was reverse transcribed with random hexamers and PCR primers were the same as in (A) and (B) .

    Techniques Used: Purification, Isolation, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Polymerase Chain Reaction, Amplification, Quantitative RT-PCR

    Long read sequencing of nascent RNA with PacBio technology yields high coverage in differentiating mouse erythroblasts (A) Schematic of nascent RNA isolation and sequencing library generation. MEL cells are treated with 2% DMSO to induce erythroid differentiation, then cells are fractionated to purify chromatin, and chromatin-associated nascent RNA is subsequently depleted of polyadenylated and ribosomal RNAs. An adapter is ligated to the 3′ ends of all remaining RNAs, then a strand-switching reverse transcriptase is used to create double-stranded cDNA that is the input for PacBio library preparation. (B) Read length distribution of PacBio long reads. (C) Read depth of PacBio long reads. For (B) and (C) , data represent two biological replicates and two technical replicates combined. See also Figures S1 , Figure S2 , and Table S1 .
    Figure Legend Snippet: Long read sequencing of nascent RNA with PacBio technology yields high coverage in differentiating mouse erythroblasts (A) Schematic of nascent RNA isolation and sequencing library generation. MEL cells are treated with 2% DMSO to induce erythroid differentiation, then cells are fractionated to purify chromatin, and chromatin-associated nascent RNA is subsequently depleted of polyadenylated and ribosomal RNAs. An adapter is ligated to the 3′ ends of all remaining RNAs, then a strand-switching reverse transcriptase is used to create double-stranded cDNA that is the input for PacBio library preparation. (B) Read length distribution of PacBio long reads. (C) Read depth of PacBio long reads. For (B) and (C) , data represent two biological replicates and two technical replicates combined. See also Figures S1 , Figure S2 , and Table S1 .

    Techniques Used: Sequencing, Isolation

    Related Articles

    Clone Assay:

    Article Title: Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits
    Article Snippet: .. Gene cloning and sequencing The LIF, Tbox 5 and MEF2C genes were amplified with a PCR kit (Takara Co.) from their respective plasmids. .. The resulting PCR products were purified with regenerated or fresh columns and using Qiagen or Axygen PCR purification kit buffers.

    Amplification:

    Article Title: Differential Gene Expression Responding to Low Phosphate Stress in Leaves and Roots of Maize by cDNA-SRAP
    Article Snippet: .. In order to reduce the amplifications of nonspecific fragments in the cDNA-SRAP analysis, the conditions of PCR amplification were optimized to be 35 cycles of PCR amplification with a 20-fold dilution of DNA template. ..

    Article Title: Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System
    Article Snippet: .. The target site of the BmNPV genome was amplified with a PCR reagent kit (Takara, Dalian, China) using the indicated primers ( Supplementary Table ). .. Then, the PCR products were reannealed in NEBuffer 2 (NEB, United States) using the following conditions: 95°C for 5 min; 95–85°C at -2°C/s; 85–25°C at -0.1°C/s; hold at 4°C, and then digested with T7 endonuclease I (T7EI) for 30 min at 37°C.

    Article Title: Study of the activation of the PI3K/Akt pathway by the motif of σA and σNS proteins of avian reovirus
    Article Snippet: .. To amplify the σA-M1 gene (σA gene mutant 1), the PCR protocol consisted of three rounds of PCR amplification. .. The first PCR amplification used σA-F and σA-M1-R primers, with σA-pcAGEN as the template; the second PCR amplification used σA-M1-F and σA-R primers, with σA-pcAGEN as the template.

    Article Title: A novel strategy for promoting homoplasmic plastid transformant production using the barnase–barstar system
    Article Snippet: .. PCR amplification of barstar and Ntrps3, which is a plastid-encoded control gene, was performed for 23 cycles at 95°C for 15 s, 60°C for 15 s, and 72°C for 20 s using the primer sets BsF1/BsR1 (5′-cagaagtatcagcgacctccac-3′/5′-gtatgatggtgatgtcgcagc-3′) and Ntrps3F/Ntrps3R (5′-ggggaaccctaccttctctg-3′/5′-ccgaaaactgaacattgctg-3′). ..

    Article Title: Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits
    Article Snippet: .. Gene cloning and sequencing The LIF, Tbox 5 and MEF2C genes were amplified with a PCR kit (Takara Co.) from their respective plasmids. .. The resulting PCR products were purified with regenerated or fresh columns and using Qiagen or Axygen PCR purification kit buffers.

    Article Title: Study of the activation of the PI3K/Akt pathway by the motif of σA and σNS proteins of avian reovirus
    Article Snippet: .. The third PCR amplification used σA-F and σA-R primers with the gel extraction purification product of the first and second PCR used as the template. ..

    Article Title: Study of the activation of the PI3K/Akt pathway by the motif of σA and σNS proteins of avian reovirus
    Article Snippet: .. The first PCR amplification used σA-F and σA-M1-R primers, with σA-pcAGEN as the template; the second PCR amplification used σA-M1-F and σA-R primers, with σA-pcAGEN as the template. ..

    Mutagenesis:

    Article Title: Study of the activation of the PI3K/Akt pathway by the motif of σA and σNS proteins of avian reovirus
    Article Snippet: .. To amplify the σA-M1 gene (σA gene mutant 1), the PCR protocol consisted of three rounds of PCR amplification. .. The first PCR amplification used σA-F and σA-M1-R primers, with σA-pcAGEN as the template; the second PCR amplification used σA-M1-F and σA-R primers, with σA-pcAGEN as the template.

    Purification:

    Article Title: Study of the activation of the PI3K/Akt pathway by the motif of σA and σNS proteins of avian reovirus
    Article Snippet: .. The third PCR amplification used σA-F and σA-R primers with the gel extraction purification product of the first and second PCR used as the template. ..

    Polymerase Chain Reaction:

    Article Title: Differential Gene Expression Responding to Low Phosphate Stress in Leaves and Roots of Maize by cDNA-SRAP
    Article Snippet: .. In order to reduce the amplifications of nonspecific fragments in the cDNA-SRAP analysis, the conditions of PCR amplification were optimized to be 35 cycles of PCR amplification with a 20-fold dilution of DNA template. ..

    Article Title: Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System
    Article Snippet: .. The target site of the BmNPV genome was amplified with a PCR reagent kit (Takara, Dalian, China) using the indicated primers ( Supplementary Table ). .. Then, the PCR products were reannealed in NEBuffer 2 (NEB, United States) using the following conditions: 95°C for 5 min; 95–85°C at -2°C/s; 85–25°C at -0.1°C/s; hold at 4°C, and then digested with T7 endonuclease I (T7EI) for 30 min at 37°C.

    Article Title: Study of the activation of the PI3K/Akt pathway by the motif of σA and σNS proteins of avian reovirus
    Article Snippet: .. To amplify the σA-M1 gene (σA gene mutant 1), the PCR protocol consisted of three rounds of PCR amplification. .. The first PCR amplification used σA-F and σA-M1-R primers, with σA-pcAGEN as the template; the second PCR amplification used σA-M1-F and σA-R primers, with σA-pcAGEN as the template.

    Article Title: A novel strategy for promoting homoplasmic plastid transformant production using the barnase–barstar system
    Article Snippet: .. PCR amplification of barstar and Ntrps3, which is a plastid-encoded control gene, was performed for 23 cycles at 95°C for 15 s, 60°C for 15 s, and 72°C for 20 s using the primer sets BsF1/BsR1 (5′-cagaagtatcagcgacctccac-3′/5′-gtatgatggtgatgtcgcagc-3′) and Ntrps3F/Ntrps3R (5′-ggggaaccctaccttctctg-3′/5′-ccgaaaactgaacattgctg-3′). ..

    Article Title: Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits
    Article Snippet: .. Gene cloning and sequencing The LIF, Tbox 5 and MEF2C genes were amplified with a PCR kit (Takara Co.) from their respective plasmids. .. The resulting PCR products were purified with regenerated or fresh columns and using Qiagen or Axygen PCR purification kit buffers.

    Article Title: Study of the activation of the PI3K/Akt pathway by the motif of σA and σNS proteins of avian reovirus
    Article Snippet: .. The third PCR amplification used σA-F and σA-R primers with the gel extraction purification product of the first and second PCR used as the template. ..

    Article Title: Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1
    Article Snippet: .. Briefly, PCR MIMICs were generated by two successive PCR amplifications according to the PCR MIMIC construction kit (Clontech Laboratories, Inc, Palo Alto, Calif). .. Four-fold dilutions of each PCR MIMIC between 10−1 –10−2 attomole/mL were added to PCR amplification reactions containing a constant amount of the sample cDNA.

    Article Title: Study of the activation of the PI3K/Akt pathway by the motif of σA and σNS proteins of avian reovirus
    Article Snippet: .. The first PCR amplification used σA-F and σA-M1-R primers, with σA-pcAGEN as the template; the second PCR amplification used σA-M1-F and σA-R primers, with σA-pcAGEN as the template. ..

    Generated:

    Article Title: Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1
    Article Snippet: .. Briefly, PCR MIMICs were generated by two successive PCR amplifications according to the PCR MIMIC construction kit (Clontech Laboratories, Inc, Palo Alto, Calif). .. Four-fold dilutions of each PCR MIMIC between 10−1 –10−2 attomole/mL were added to PCR amplification reactions containing a constant amount of the sample cDNA.

    Sequencing:

    Article Title: Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits
    Article Snippet: .. Gene cloning and sequencing The LIF, Tbox 5 and MEF2C genes were amplified with a PCR kit (Takara Co.) from their respective plasmids. .. The resulting PCR products were purified with regenerated or fresh columns and using Qiagen or Axygen PCR purification kit buffers.

    Gel Extraction:

    Article Title: Study of the activation of the PI3K/Akt pathway by the motif of σA and σNS proteins of avian reovirus
    Article Snippet: .. The third PCR amplification used σA-F and σA-R primers with the gel extraction purification product of the first and second PCR used as the template. ..

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    TaKaRa complementary dna template generated by the reverse transcription kit takara
    Complementary Dna Template Generated By The Reverse Transcription Kit Takara, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complementary dna template generated by the reverse transcription kit takara/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complementary dna template generated by the reverse transcription kit takara - by Bioz Stars, 2020-09
    94/100 stars
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