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Nugen complementary dna cdna
Complementary Dna Cdna, supplied by Nugen, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complementary dna cdna/product/Nugen
Average 93 stars, based on 16 article reviews
Price from $9.99 to $1999.99
complementary dna cdna - by Bioz Stars, 2020-08
93/100 stars

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Amplification:

Article Title: Characterization of the molecular mechanisms underlying increased ischemic damage in the aldehyde dehydrogenase 2 genetic polymorphism using a human induced pluripotent stem cell model system
Article Snippet: .. RNA was isolated from ALDH2*2/1 and wild-type control iPSC-CMs ( n = 2 per group) using a Qiagen microRNA kit, and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp, and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). ..

Article Title: Single-cell analysis of the fate of c-kit-positive bone marrow cells
Article Snippet: .. RNA was isolated using an RNeasy mini kit (Qiagen), and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). .. Sequencing was performed using Illumina’s HiSeq2000 platform using paired in reads at an average length of 100 bp.

Article Title: Anti-LINGO-1 has no detectable immunomodulatory effects in preclinical and phase 1 studies
Article Snippet: .. For CSF RNA samples, total RNA (1–5 ng) was reverse transcribed and amplified to complementary DNA (cDNA) using the Ovation Pico WTA System V2 (NuGEN Technologies Inc.); the cDNA samples were then treated as per the blood RNA. .. For blood and CSF samples, 2-µg biotin-labeled cDNA probe was hybridized to Affymetrix GeneChip HT HG-U133+ PM array plates (Thermo Fisher Scientific Inc.), using modified conditions.

RNA Sequencing Assay:

Article Title: Analyzing allele specific RNA expression using mixture models
Article Snippet: .. Complementary DNA (cDNA) was reverse transcribed from 25 ng total RNA using the Ovation RNA-Seq System v2 (NuGen), which suppresses ribosomal RNA conversion to cDNA and employs both poly-dT and random hexamer primers, capturing all RNA species (including non-poly-adenylated RNAs and intronic fragments). .. This cDNA was used to construct libraries for massively parallel sequencing using the NEBNext DNA Library Prep Set for SOLiD (New England Biolabs, NEB, Ipswich, MA), per manufacturer’s instructions.

Article Title: Zic3 is required in the migrating primitive streak for node morphogenesis and left-right patterning
Article Snippet: .. Total purified RNA samples were converted to Complementary DNA (cDNA) using the Ovation RNA-seq System v2 assay (NuGEN Technologies). .. RT–PCR was performed on cDNA using the following primers: Zic3 F-5′ CGG GCT GCG GGA AGA T-3′; Zic3 R-5′ CTC ACC TGT ATG GGT CCT CTT GT-3′; Gapdh F-5′ TGC GAC TTC AAC AGC AAC TC -3′ and Gapdh R-5′ GCC TCT CTT GCT CAG TGT CC-3′.

Article Title: Characterization of the molecular mechanisms underlying increased ischemic damage in the aldehyde dehydrogenase 2 genetic polymorphism using a human induced pluripotent stem cell model system
Article Snippet: .. RNA was isolated from ALDH2*2/1 and wild-type control iPSC-CMs ( n = 2 per group) using a Qiagen microRNA kit, and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp, and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). ..

Article Title: Single-cell analysis of the fate of c-kit-positive bone marrow cells
Article Snippet: .. RNA was isolated using an RNeasy mini kit (Qiagen), and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). .. Sequencing was performed using Illumina’s HiSeq2000 platform using paired in reads at an average length of 100 bp.

Article Title: A DLL3-targeted antibody-drug conjugate eradicates high-grade pulmonary neuroendocrine tumor-initiating cells in vivo
Article Snippet: .. For whole transcriptome analysis, complementary DNA (cDNA) was generated from 1 ng of RNA using the Ovation RNA-Seq System V2 (NuGEN Technologies). .. The resulting cDNA library was fragmented, and barcode adapters were added to allow pooling of fragment libraries from different samples.

Isolation:

Article Title: Characterization of the molecular mechanisms underlying increased ischemic damage in the aldehyde dehydrogenase 2 genetic polymorphism using a human induced pluripotent stem cell model system
Article Snippet: .. RNA was isolated from ALDH2*2/1 and wild-type control iPSC-CMs ( n = 2 per group) using a Qiagen microRNA kit, and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp, and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). ..

Article Title: Single-cell analysis of the fate of c-kit-positive bone marrow cells
Article Snippet: .. RNA was isolated using an RNeasy mini kit (Qiagen), and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). .. Sequencing was performed using Illumina’s HiSeq2000 platform using paired in reads at an average length of 100 bp.

Random Hexamer Labeling:

Article Title: Analyzing allele specific RNA expression using mixture models
Article Snippet: .. Complementary DNA (cDNA) was reverse transcribed from 25 ng total RNA using the Ovation RNA-Seq System v2 (NuGen), which suppresses ribosomal RNA conversion to cDNA and employs both poly-dT and random hexamer primers, capturing all RNA species (including non-poly-adenylated RNAs and intronic fragments). .. This cDNA was used to construct libraries for massively parallel sequencing using the NEBNext DNA Library Prep Set for SOLiD (New England Biolabs, NEB, Ipswich, MA), per manufacturer’s instructions.

Purification:

Article Title: Zic3 is required in the migrating primitive streak for node morphogenesis and left-right patterning
Article Snippet: .. Total purified RNA samples were converted to Complementary DNA (cDNA) using the Ovation RNA-seq System v2 assay (NuGEN Technologies). .. RT–PCR was performed on cDNA using the following primers: Zic3 F-5′ CGG GCT GCG GGA AGA T-3′; Zic3 R-5′ CTC ACC TGT ATG GGT CCT CTT GT-3′; Gapdh F-5′ TGC GAC TTC AAC AGC AAC TC -3′ and Gapdh R-5′ GCC TCT CTT GCT CAG TGT CC-3′.

Generated:

Article Title: Regional Expression of the BCRP/ABCG2 Transporter in Term Human Placentas
Article Snippet: .. Complementary DNA (cDNA) was generated using the Ovation Pico WTA System (NuGEN, San Carlos, CA) in combination with the Biomek FXP Laboratory Automation Workstation (Beckman Coulter, Inc., Brea, CA). .. Quantitative analysis of mRNA was performed with 500 ng cDNA, commercially-available primers for each gene, and Taqman probes (Applied Biosystems, Foster City, CA) using a ViiA7 RT-PCR system (Applied Biosystems) in the Bionomics Research and Technology Center at Rutgers University.

Article Title: A DLL3-targeted antibody-drug conjugate eradicates high-grade pulmonary neuroendocrine tumor-initiating cells in vivo
Article Snippet: .. For whole transcriptome analysis, complementary DNA (cDNA) was generated from 1 ng of RNA using the Ovation RNA-Seq System V2 (NuGEN Technologies). .. The resulting cDNA library was fragmented, and barcode adapters were added to allow pooling of fragment libraries from different samples.

Sequencing:

Article Title: Characterization of the molecular mechanisms underlying increased ischemic damage in the aldehyde dehydrogenase 2 genetic polymorphism using a human induced pluripotent stem cell model system
Article Snippet: .. RNA was isolated from ALDH2*2/1 and wild-type control iPSC-CMs ( n = 2 per group) using a Qiagen microRNA kit, and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp, and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). ..

Article Title: Single-cell analysis of the fate of c-kit-positive bone marrow cells
Article Snippet: .. RNA was isolated using an RNeasy mini kit (Qiagen), and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). .. Sequencing was performed using Illumina’s HiSeq2000 platform using paired in reads at an average length of 100 bp.

Sonication:

Article Title: Characterization of the molecular mechanisms underlying increased ischemic damage in the aldehyde dehydrogenase 2 genetic polymorphism using a human induced pluripotent stem cell model system
Article Snippet: .. RNA was isolated from ALDH2*2/1 and wild-type control iPSC-CMs ( n = 2 per group) using a Qiagen microRNA kit, and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp, and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). ..

Article Title: Single-cell analysis of the fate of c-kit-positive bone marrow cells
Article Snippet: .. RNA was isolated using an RNeasy mini kit (Qiagen), and 100 ng of total RNA was converted to complementary DNA (cDNA) and amplified using NuGEN V2 RNA-Seq kit (NuGEN). cDNA was sonicated to an average fragment size of 300 bp and Illumina sequencing adapters were ligated to 500 ng of cDNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs). .. Sequencing was performed using Illumina’s HiSeq2000 platform using paired in reads at an average length of 100 bp.

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  • Bioz Stars
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  • 92
    Nugen cdna preparation
    Categorization of RNA-Seq reads. ( A ) Alignment of 24,202,967 RNA-Seq reads to a P. syringae reference genome sequence. The rRNAs were depleted using Ribominus and MicrobExpress. The remaining RNA were converted to <t>cDNA</t> and sequenced on an Illumina IIG using single-direction 40-cycle sequencing. The first 10 and last five bases of each read were trimmed off. The 25 mers were pooled across six samples and aligned using the alignment program, CASHX version 2.3, allowing up to two mismatches. Reads were categorized based on alignment to a unique position (Mapped), the <t>rRNA-encoding</t> locus (Mapped to rRNA), failure to align (Not Mapped), and alignment to multiple locations in the reference genome sequence (Ambiguously Mapped). ( B ) Distribution and frequency of 25 mer RNA-Seq reads that aligned to the rRNA-encoding locus of P. syringae following rRNA-depletion. Reads were aligned using CASHX version 2.3.
    Cdna Preparation, supplied by Nugen, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna preparation/product/Nugen
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    cdna preparation - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Nugen cdna synthesis
    ( A ) Comparison of average mapping statistics of transcriptomic content among three datasets using 50 bp reads from Genome Analyzer IIx (Illumina, Inc.): <t>cDNA</t> libraries made from single <t>RNA</t> samples of mouse testis tissue in which mRNA was enriched from total RNA either by (i) TruSeq™ poly-A selection or by (ii) RiboMinus™ rRNA depletion and (iii) cDNA library in which cDNA was synthesized directly from total RNA (DNase treated and left untreated) using the Ovation® RNA-Seq system. ( B ) Complexity of libraries (percent of unique start sites, unique pairs out of total mapped reads): cDNA libraries of TruSeq™ poly-A selection, RiboMinus™ rRNA depletion and Ovation® RNA-seq amplification system (different input amounts of total RNA). The sample prepared using the Ovation® RNA-Seq system provided slightly higher percentage of unique start sites.
    Cdna Synthesis, supplied by Nugen, used in various techniques. Bioz Stars score: 92/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis/product/Nugen
    Average 92 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Categorization of RNA-Seq reads. ( A ) Alignment of 24,202,967 RNA-Seq reads to a P. syringae reference genome sequence. The rRNAs were depleted using Ribominus and MicrobExpress. The remaining RNA were converted to cDNA and sequenced on an Illumina IIG using single-direction 40-cycle sequencing. The first 10 and last five bases of each read were trimmed off. The 25 mers were pooled across six samples and aligned using the alignment program, CASHX version 2.3, allowing up to two mismatches. Reads were categorized based on alignment to a unique position (Mapped), the rRNA-encoding locus (Mapped to rRNA), failure to align (Not Mapped), and alignment to multiple locations in the reference genome sequence (Ambiguously Mapped). ( B ) Distribution and frequency of 25 mer RNA-Seq reads that aligned to the rRNA-encoding locus of P. syringae following rRNA-depletion. Reads were aligned using CASHX version 2.3.

    Journal: Genes

    Article Title: RNA-Seq for Plant Pathogenic Bacteria

    doi: 10.3390/genes2040689

    Figure Lengend Snippet: Categorization of RNA-Seq reads. ( A ) Alignment of 24,202,967 RNA-Seq reads to a P. syringae reference genome sequence. The rRNAs were depleted using Ribominus and MicrobExpress. The remaining RNA were converted to cDNA and sequenced on an Illumina IIG using single-direction 40-cycle sequencing. The first 10 and last five bases of each read were trimmed off. The 25 mers were pooled across six samples and aligned using the alignment program, CASHX version 2.3, allowing up to two mismatches. Reads were categorized based on alignment to a unique position (Mapped), the rRNA-encoding locus (Mapped to rRNA), failure to align (Not Mapped), and alignment to multiple locations in the reference genome sequence (Ambiguously Mapped). ( B ) Distribution and frequency of 25 mer RNA-Seq reads that aligned to the rRNA-encoding locus of P. syringae following rRNA-depletion. Reads were aligned using CASHX version 2.3.

    Article Snippet: A third and relatively new method uses enrichment by relying on “not so random” oligonucleotides during cDNA preparation to bias towards non-rRNA transcripts [ ] (Ovation® Prokaryotic RNA-Seq System; NuGen, San Carlos, CA [ ]).

    Techniques: RNA Sequencing Assay, Sequencing

    Gene-wise allelic RNA expression ratio comparisons measured by RNA-Seq (Standard, IUPAC, or Allele-Switch) versus SNaPshot (NuGen or GSP). The two alternative mapping methods (IUPAC and Allele-Switch) were similar and more highly correlated with SNaPshot allelic ratio measures for both NuGen cDNA (A) and GSP cDNA (B) , whereas Standard mapping was much less correlated (dotted line).

    Journal: BMC Genomics

    Article Title: Whole transcriptome RNA-Seq allelic expression in human brain

    doi: 10.1186/1471-2164-14-571

    Figure Lengend Snippet: Gene-wise allelic RNA expression ratio comparisons measured by RNA-Seq (Standard, IUPAC, or Allele-Switch) versus SNaPshot (NuGen or GSP). The two alternative mapping methods (IUPAC and Allele-Switch) were similar and more highly correlated with SNaPshot allelic ratio measures for both NuGen cDNA (A) and GSP cDNA (B) , whereas Standard mapping was much less correlated (dotted line).

    Article Snippet: Divergent allelic ratios between these two methods can result from an admixture of plus and minus strand-encoded RNA transcripts in the NuGen cDNA, while GSP cDNA enriches for only one strand, compelling a direct comparison between the two methods.

    Techniques: RNA Expression, RNA Sequencing Assay

    ( A ) Comparison of average mapping statistics of transcriptomic content among three datasets using 50 bp reads from Genome Analyzer IIx (Illumina, Inc.): cDNA libraries made from single RNA samples of mouse testis tissue in which mRNA was enriched from total RNA either by (i) TruSeq™ poly-A selection or by (ii) RiboMinus™ rRNA depletion and (iii) cDNA library in which cDNA was synthesized directly from total RNA (DNase treated and left untreated) using the Ovation® RNA-Seq system. ( B ) Complexity of libraries (percent of unique start sites, unique pairs out of total mapped reads): cDNA libraries of TruSeq™ poly-A selection, RiboMinus™ rRNA depletion and Ovation® RNA-seq amplification system (different input amounts of total RNA). The sample prepared using the Ovation® RNA-Seq system provided slightly higher percentage of unique start sites.

    Journal: Nucleic Acids Research

    Article Title: Whole-transcriptome RNAseq analysis from minute amount of total RNA

    doi: 10.1093/nar/gkr547

    Figure Lengend Snippet: ( A ) Comparison of average mapping statistics of transcriptomic content among three datasets using 50 bp reads from Genome Analyzer IIx (Illumina, Inc.): cDNA libraries made from single RNA samples of mouse testis tissue in which mRNA was enriched from total RNA either by (i) TruSeq™ poly-A selection or by (ii) RiboMinus™ rRNA depletion and (iii) cDNA library in which cDNA was synthesized directly from total RNA (DNase treated and left untreated) using the Ovation® RNA-Seq system. ( B ) Complexity of libraries (percent of unique start sites, unique pairs out of total mapped reads): cDNA libraries of TruSeq™ poly-A selection, RiboMinus™ rRNA depletion and Ovation® RNA-seq amplification system (different input amounts of total RNA). The sample prepared using the Ovation® RNA-Seq system provided slightly higher percentage of unique start sites.

    Article Snippet: For comparison studies between mRNA enrichment methods and the Ovation® RNA-Seq system, a single 100 ng total RNA sample (with technical replicate) was processed for cDNA synthesis using the Ovation® RNA-Seq system (NuGEN Technologies, Inc.; San Carlos, CA, USA), and either DNase-treated using DNase mix from RecoverAllTM Total Nucleic Acid Isolation kit (Applied Biosystems/Ambion, Austin, TX, USA) or left untreated.

    Techniques: Selection, cDNA Library Assay, Synthesized, RNA Sequencing Assay, Amplification

    ( A ) Average percentile coverage across all transcripts showing significant 3′ bias in the TruSeq™ poly-A selection method, slight 5′ bias in the RiboMinus™ rRNA depletion method, and slight 3′ bias in the Ovation® RNA-Seq system as assessed by single RNA samples of mouse testis tissues. There was no noticeable difference for transcript coverage between libraries prepared from non-sheared and sheared cDNA for the Ovation® RNA-Seq system. ( B ) Transcripts with coverage at extreme ends (5′ and 3′) confirming 3′ bias for TruSeq™ poly-A selection method, slight 5′ bias for RiboMinus™ and slight 3′ bias for Ovation® RNA-Seq system in single RNA sample of mouse testis tissues. Each line represents number of highly expressed transcripts with coverage at extreme ends over the total number of highly expressed transcripts at a base-level resolution.

    Journal: Nucleic Acids Research

    Article Title: Whole-transcriptome RNAseq analysis from minute amount of total RNA

    doi: 10.1093/nar/gkr547

    Figure Lengend Snippet: ( A ) Average percentile coverage across all transcripts showing significant 3′ bias in the TruSeq™ poly-A selection method, slight 5′ bias in the RiboMinus™ rRNA depletion method, and slight 3′ bias in the Ovation® RNA-Seq system as assessed by single RNA samples of mouse testis tissues. There was no noticeable difference for transcript coverage between libraries prepared from non-sheared and sheared cDNA for the Ovation® RNA-Seq system. ( B ) Transcripts with coverage at extreme ends (5′ and 3′) confirming 3′ bias for TruSeq™ poly-A selection method, slight 5′ bias for RiboMinus™ and slight 3′ bias for Ovation® RNA-Seq system in single RNA sample of mouse testis tissues. Each line represents number of highly expressed transcripts with coverage at extreme ends over the total number of highly expressed transcripts at a base-level resolution.

    Article Snippet: For comparison studies between mRNA enrichment methods and the Ovation® RNA-Seq system, a single 100 ng total RNA sample (with technical replicate) was processed for cDNA synthesis using the Ovation® RNA-Seq system (NuGEN Technologies, Inc.; San Carlos, CA, USA), and either DNase-treated using DNase mix from RecoverAllTM Total Nucleic Acid Isolation kit (Applied Biosystems/Ambion, Austin, TX, USA) or left untreated.

    Techniques: Selection, RNA Sequencing Assay

    ( A ) Sensitivity analysis of the Ovation® RNA-Seq system showing almost equal percentage of genes detected ( > 1× coverage) from total RNA input > 500 pg, slightly fewer genes for 500 pg and significantly fewer for 50 pg total input RNA. ( B ) Spearman's correlation is shown for all samples used in the sensitivity analysis and comparison studies between cDNA synthesis methods. The Ovation® RNA-Seq system samples with > 500 pg show good agreement with one another. RiboMinus™ replicates share higher agreement to one of the replicate of TruSeq™ selection than to Ovation® samples.

    Journal: Nucleic Acids Research

    Article Title: Whole-transcriptome RNAseq analysis from minute amount of total RNA

    doi: 10.1093/nar/gkr547

    Figure Lengend Snippet: ( A ) Sensitivity analysis of the Ovation® RNA-Seq system showing almost equal percentage of genes detected ( > 1× coverage) from total RNA input > 500 pg, slightly fewer genes for 500 pg and significantly fewer for 50 pg total input RNA. ( B ) Spearman's correlation is shown for all samples used in the sensitivity analysis and comparison studies between cDNA synthesis methods. The Ovation® RNA-Seq system samples with > 500 pg show good agreement with one another. RiboMinus™ replicates share higher agreement to one of the replicate of TruSeq™ selection than to Ovation® samples.

    Article Snippet: For comparison studies between mRNA enrichment methods and the Ovation® RNA-Seq system, a single 100 ng total RNA sample (with technical replicate) was processed for cDNA synthesis using the Ovation® RNA-Seq system (NuGEN Technologies, Inc.; San Carlos, CA, USA), and either DNase-treated using DNase mix from RecoverAllTM Total Nucleic Acid Isolation kit (Applied Biosystems/Ambion, Austin, TX, USA) or left untreated.

    Techniques: RNA Sequencing Assay, Selection

    Amplification of cDNA, where a – c represents three replications of syncytial samples processed after LCM. The virtual gel generated by an Agilent 2100 Bioanalyzer is shown in d . nt nucelotide, bp base pair

    Journal: Plant Methods

    Article Title: An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection

    doi: 10.1186/s13007-016-0123-9

    Figure Lengend Snippet: Amplification of cDNA, where a – c represents three replications of syncytial samples processed after LCM. The virtual gel generated by an Agilent 2100 Bioanalyzer is shown in d . nt nucelotide, bp base pair

    Article Snippet: Agilent, Germany). cDNA synthesis was done with NuGEN’s Ovation Pico WTA System (Cat. No. 3302-12.

    Techniques: Amplification, Laser Capture Microdissection, Generated