top10 competent e coli  (Thermo Fisher)


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    Name:
    Competent Cell Sampler
    Description:
    Our Competent Cell Sampler includes two reactions of four chemically competent E coli strains One Shot Mach1 T1R catalog C862003 is the fastest growing chemically competent strain currently available Mach1 T1R colonies are clearly visible within eight hours of plating your transformation From an overnight colony minipreps can be performed after four hours of growth Genotype F Φ80lacZΔM15 ΔlacX74 hsdR rK mK ΔrecA1398 endA1 tonA One Shot OmniMAX 2 T1R cells catalog C854003 are perfect for use in all cloning applications including Gateway technology They offer the highest transformation efficiency of any chemically competent cell in the One Shot packaging format Genotype F proAB lacIq lacZΔM15 Tn10 TetR Δ ccdAB mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 Δ lacZYA argF U169 endA1 recA1 supE44 thi 1 gyrA96 relA1 tonA panD One Shot Stbl3 catalog C7373 03 reduce the frequency of unwanted homologous recombination and so are perfect for cloning unstable DNA Genotype F mcrB mrr hsdS20 rB mB recA13 supE44 ara 14 galK2 lacY1 proA2 rpsL20 StrR xyl 5 leu mtl 1 One Shot TOP10 cells C4040 03 are perfect for routine cloning and included in many TOPO cloning and expression kits Genotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ ara leu 7697 galU galK rpsL StrR endA1 nupGTry them all to optimize transformations for your favorite clones
    Catalog Number:
    a10469
    Price:
    None
    Applications:
    Chemically Competent Cells for Cloning|Cloning|Transformation
    Category:
    Competent Cells Strains
    Buy from Supplier


    Structured Review

    Thermo Fisher top10 competent e coli
    Our Competent Cell Sampler includes two reactions of four chemically competent E coli strains One Shot Mach1 T1R catalog C862003 is the fastest growing chemically competent strain currently available Mach1 T1R colonies are clearly visible within eight hours of plating your transformation From an overnight colony minipreps can be performed after four hours of growth Genotype F Φ80lacZΔM15 ΔlacX74 hsdR rK mK ΔrecA1398 endA1 tonA One Shot OmniMAX 2 T1R cells catalog C854003 are perfect for use in all cloning applications including Gateway technology They offer the highest transformation efficiency of any chemically competent cell in the One Shot packaging format Genotype F proAB lacIq lacZΔM15 Tn10 TetR Δ ccdAB mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 Δ lacZYA argF U169 endA1 recA1 supE44 thi 1 gyrA96 relA1 tonA panD One Shot Stbl3 catalog C7373 03 reduce the frequency of unwanted homologous recombination and so are perfect for cloning unstable DNA Genotype F mcrB mrr hsdS20 rB mB recA13 supE44 ara 14 galK2 lacY1 proA2 rpsL20 StrR xyl 5 leu mtl 1 One Shot TOP10 cells C4040 03 are perfect for routine cloning and included in many TOPO cloning and expression kits Genotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ ara leu 7697 galU galK rpsL StrR endA1 nupGTry them all to optimize transformations for your favorite clones
    https://www.bioz.com/result/top10 competent e coli/product/Thermo Fisher
    Average 90 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    top10 competent e coli - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Isolation of Highly Active Monoclonal Antibodies against Multiresistant Gram-Positive Bacteria
    Article Snippet: Paragraph title: Cloning of variable domains into eukaryotic expression vector TCAE6.7 ... The ligation reaction mixture was transformed into competent E. coli TOP10 cells (Invitrogen) and plasmids were purified using a plasmid Miniprep kit (QIAGEN).

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: The cDNA encoding FpPG was cloned in pGAPZαA (Invitrogen) using the Eco RI and Xba I restriction sites introduced by using the primers EcoFw (5′-acctga gaattc gatccctgctccgtgac-3′) and XbaRv (5′-gccta tctaga ctagctggggcaagtgtt-3′). .. The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells.

    Article Title: A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica
    Article Snippet: .. Chemically competent E. coli Mach1TM cells (Life Technologies, Inc., Grand Island, NY) were used for all cloning procedures. .. Bacteria were grown in Miller’s modified Luria Broth (LB) or on Miller’s Luria agar medium (Research Products International Corp.) unless otherwise specified.

    Article Title: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses
    Article Snippet: The resulting plasmid was named as pTN, is a high-copy number, pUC19-based vector contains a multiple cloning site (MCS) between the hyperactive 19 bp Mosaic Ends (ME) that is specifically and uniquely recognized by Tn5 Transposase. .. The resulting plasmid, designated as pTN-HMY (Figure ), was then transformed into chemically competent E. coli Top10 cells (Invitrogen) and inoculated onto LB agar plates supplemented with tetracycline and ampicillin and sequence confirmed.

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: We used the Advantage 2 PCR kit from Clontech (Mountain View, USA) to amplify the coding sequence from TL zebrafish liver cDNA with 1 μM of each primer; the thermal profile was: 94°C for 1 min, [94°C for 30 sec, 58°C for 3 min] for 35 cycles, and 68°C for 7 min. PCR products were purified with the QIAquick Gel Extraction Kit from Qiagen (Valencia, USA) and cloned into pGEM-T Easy from Promega (Madison, USA) as per kit instructions. .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA).

    Amplification:

    Article Title: Inflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms
    Article Snippet: To generate pLPN15 and pLPN16, the firefly-luciferase gene luc from pLB02 was amplified with Luc-for-Bam HI and Luc-rev-Hind III primers ( ) and inserted into Bam HI/Hind III digested pLPN1 or pM1437, respectively. .. Four microliters of the Gibson assembly mix were transformed into chemically competent E. coli Mach1 T1 cells (Life Technologies).

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Products were amplified by PCR under the following conditions: initial incubation (3 minutes in 94°C), then 30 cycles of denaturation (30 seconds in 94°C), annealing (1 minute in 57°C), and elongation (1 minute in 72°C). .. Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen).

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: .. The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells. .. Transformants were selected on low-salt Luria-Bertani plates containing 25 μg/mL zeocin (Duchefa Biochemie) and analyzed by direct PCR amplification using specific primers of the α-factor signal peptide and the 3′-Alcohol Oxidase terminator sequence according to the manufacturer’s instruction.

    Article Title: The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae
    Article Snippet: Fusion PCR was then used to combine upstream and downstream products with a 660 bp amplicon of the cat gene ( ). .. This vector was then used to transform chemically competent Mach 1 E. coli cells (Invitrogen).

    Stable Transfection:

    Article Title: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses
    Article Snippet: The resulting plasmid, designated as pTN-HMY (Figure ), was then transformed into chemically competent E. coli Top10 cells (Invitrogen) and inoculated onto LB agar plates supplemented with tetracycline and ampicillin and sequence confirmed. .. The mixture was incubated for 30 min at room temperature, to allow the transposase to stably bind to the Tn5 Transposon DNA; that mixture was then stored at -20°C.

    Synthesized:

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Specific primers were synthesized according to the sequences of the mature protein (without the signal peptide) ( ). .. Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen).

    TA Cloning:

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: .. Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). ..

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again.

    Construct:

    Article Title: Inflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms
    Article Snippet: Four microliters of the Gibson assembly mix were transformed into chemically competent E. coli Mach1 T1 cells (Life Technologies). .. Constructs were verified using colony PCR, restriction analysis and sequencing.

    Article Title: Isolation of Highly Active Monoclonal Antibodies against Multiresistant Gram-Positive Bacteria
    Article Snippet: Heavy (H) chain V-region genes from the four constructs were digested with SalI and NheI restriction enzymes (NEB) and ligated into TCAE6.7 cut with the same enzymes. .. The ligation reaction mixture was transformed into competent E. coli TOP10 cells (Invitrogen) and plasmids were purified using a plasmid Miniprep kit (QIAGEN).

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: .. The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells. .. Transformants were selected on low-salt Luria-Bertani plates containing 25 μg/mL zeocin (Duchefa Biochemie) and analyzed by direct PCR amplification using specific primers of the α-factor signal peptide and the 3′-Alcohol Oxidase terminator sequence according to the manufacturer’s instruction.

    Article Title: The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae
    Article Snippet: Primers adjacent to the upstream and downstream regions of pdi were constructed with 25 bp 5′ extensions corresponding to the 5′ and 3′ ends of the cat gene from pACYC ( ). .. This vector was then used to transform chemically competent Mach 1 E. coli cells (Invitrogen).

    Article Title: Genome-Wide Transposon Mutagenesis of Borrelia burgdorferi for Identification of Phenotypic Mutants
    Article Snippet: Vector pMarGent was constructed such that the transposon and B. burgdorferi DNA flanking the insertion site could be rescued easily in E. coli (Fig. and ). .. Transposon insertions along with B. burgdorferi flanking DNA were recovered by transforming 5 μl of the ligation reaction mixture into chemically competent E. coli TOP10 cells (Invitrogen).

    Incubation:

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Products were amplified by PCR under the following conditions: initial incubation (3 minutes in 94°C), then 30 cycles of denaturation (30 seconds in 94°C), annealing (1 minute in 57°C), and elongation (1 minute in 72°C). .. Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen).

    Article Title: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses
    Article Snippet: The resulting plasmid, designated as pTN-HMY (Figure ), was then transformed into chemically competent E. coli Top10 cells (Invitrogen) and inoculated onto LB agar plates supplemented with tetracycline and ampicillin and sequence confirmed. .. The mixture was incubated for 30 min at room temperature, to allow the transposase to stably bind to the Tn5 Transposon DNA; that mixture was then stored at -20°C.

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA). .. Three clones were sent to Eurofins MWG Operon (Louisville, USA) for forward and reverse sequencing with T7 primers (1 μg of plasmid DNA), and the full-length sequence was then assembled and aligned with [GenBank: ] using MacVector 12.0.2 (Oxford Molecular Group, Madison, USA).

    Expressing:

    Article Title: Isolation of Highly Active Monoclonal Antibodies against Multiresistant Gram-Positive Bacteria
    Article Snippet: Paragraph title: Cloning of variable domains into eukaryotic expression vector TCAE6.7 ... The ligation reaction mixture was transformed into competent E. coli TOP10 cells (Invitrogen) and plasmids were purified using a plasmid Miniprep kit (QIAGEN).

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Recombinant proteins preparation Five proteins from Ph . orientalis salivary glands were chosen for expression in E . coli : PorSP15, PorSP24, PorSP65, PorSP67, and PorSP76 (in [ ] marked as PorASP15, PorMSP24, PorMSP65, and PorMSP67, and PorASP76, respectively) ( ). .. Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen).

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: Paragraph title: FpPG Expression and Purification ... The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again. .. Plasmids were isolated from the bacteria, and transfected into E . coli BL21 (DE3) gold (Agilent) for expression.

    Article Title: Antioxidant Efficacy and Adhesion Rescue by a Recombinant Mussel Foot Protein-6
    Article Snippet: E. coli One Shot TOP10 chemically competent cells [ F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 Δlacχ74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG λ- ] (Life Technologies Grand Island, NY) were used for recombinant plasmid construction. .. E. coli Rosetta 2 (DE3) cells [F− ompT hsdS B (rB − mB − ) gal dcm (DE3) pRARE (CamR )] were used as a host strain for expressing recombinant rmfp-6.1.

    Modification:

    Article Title: A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica
    Article Snippet: Chemically competent E. coli Mach1TM cells (Life Technologies, Inc., Grand Island, NY) were used for all cloning procedures. .. Bacteria were grown in Miller’s modified Luria Broth (LB) or on Miller’s Luria agar medium (Research Products International Corp.) unless otherwise specified.

    Article Title: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses
    Article Snippet: Tn5-carrying plasmid pTN was modified further for integration of foreign DNA into Z. mobilis . .. The resulting plasmid, designated as pTN-HMY (Figure ), was then transformed into chemically competent E. coli Top10 cells (Invitrogen) and inoculated onto LB agar plates supplemented with tetracycline and ampicillin and sequence confirmed.

    Transformation Assay:

    Article Title: Inflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms
    Article Snippet: .. Four microliters of the Gibson assembly mix were transformed into chemically competent E. coli Mach1 T1 cells (Life Technologies). .. Constructs were verified using colony PCR, restriction analysis and sequencing.

    Article Title: Isolation of Highly Active Monoclonal Antibodies against Multiresistant Gram-Positive Bacteria
    Article Snippet: .. The ligation reaction mixture was transformed into competent E. coli TOP10 cells (Invitrogen) and plasmids were purified using a plasmid Miniprep kit (QIAGEN). .. For light (L) chains, variable domains of the light chain cloned into the TOPO cloning vector 2.1 were digested with BglII and AvrII restriction enzymes (NEB) and ligated with the TCAE6.7 vector already containing the matching H chain variable region and cut with the same enzymes.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again. .. Plasmids were isolated from the bacteria, and transfected into E . coli BL21 (DE3) gold (Agilent) for expression.

    Article Title: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses
    Article Snippet: .. The resulting plasmid, designated as pTN-HMY (Figure ), was then transformed into chemically competent E. coli Top10 cells (Invitrogen) and inoculated onto LB agar plates supplemented with tetracycline and ampicillin and sequence confirmed. ..

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA). .. Three clones were sent to Eurofins MWG Operon (Louisville, USA) for forward and reverse sequencing with T7 primers (1 μg of plasmid DNA), and the full-length sequence was then assembled and aligned with [GenBank: ] using MacVector 12.0.2 (Oxford Molecular Group, Madison, USA).

    Article Title: Development of novel O-polysaccharide based glycoconjugates for immunization against glanders
    Article Snippet: .. Chemically competent E. coli TOP10 cells were transformed as per the manufacturer's instructions (Invitrogen). .. Oligonucleotide primers were obtained from Integrated DNA Technologies (Coralville, IA).

    Over Expression:

    Article Title: Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: the ethanolamine and choline kinases
    Article Snippet: .. Escherichia coli strains DH5α, XL-1 blue and TOP10 (Invitrogen) competent cells were used for routine manipulation, and strain BL21-Gold(DE3) (Novagen) for protein overexpression. .. All chemicals were purchased from either Sigma–Aldrich or Fluka.

    Electroporation:

    Article Title: The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae
    Article Snippet: This vector was then used to transform chemically competent Mach 1 E. coli cells (Invitrogen). .. After its propagation in MC1061 E. coli , the pKOpdi construct was introduced into WT S. iniae through electroporation.

    Transfection:

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: .. Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). ..

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again.

    Ligation:

    Article Title: Isolation of Highly Active Monoclonal Antibodies against Multiresistant Gram-Positive Bacteria
    Article Snippet: .. The ligation reaction mixture was transformed into competent E. coli TOP10 cells (Invitrogen) and plasmids were purified using a plasmid Miniprep kit (QIAGEN). .. For light (L) chains, variable domains of the light chain cloned into the TOPO cloning vector 2.1 were digested with BglII and AvrII restriction enzymes (NEB) and ligated with the TCAE6.7 vector already containing the matching H chain variable region and cut with the same enzymes.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: .. Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). ..

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again. .. Plasmids were isolated from the bacteria, and transfected into E . coli BL21 (DE3) gold (Agilent) for expression.

    Article Title: Genome-Wide Transposon Mutagenesis of Borrelia burgdorferi for Identification of Phenotypic Mutants
    Article Snippet: .. Transposon insertions along with B. burgdorferi flanking DNA were recovered by transforming 5 μl of the ligation reaction mixture into chemically competent E. coli TOP10 cells (Invitrogen). .. Individual E. coli transformants were inoculated into 5-ml cultures, and plasmid DNA was isolated using the Qiaprep Spin Miniprep kit (QIAGEN, Valencia, Calif.).

    Low Copy Number:

    Article Title: Inflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms
    Article Snippet: Primers pWSK29-Gbs-for and pWSK29-Gbs-rev were used in a PCR with pWSK29 as a template to amplify the low-copy-number plasmid ( ). .. Four microliters of the Gibson assembly mix were transformed into chemically competent E. coli Mach1 T1 cells (Life Technologies).

    Generated:

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: .. The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells. .. Transformants were selected on low-salt Luria-Bertani plates containing 25 μg/mL zeocin (Duchefa Biochemie) and analyzed by direct PCR amplification using specific primers of the α-factor signal peptide and the 3′-Alcohol Oxidase terminator sequence according to the manufacturer’s instruction.

    other:

    Article Title: Determination of Aspergillus pathogens in agricultural products by a specific nanobody-polyclonal antibody sandwich ELISA
    Article Snippet: Escherichia coli ER2738 competent cells were purchased from Lucigen Corp. (Middleton, WI, USA), Top 10 F′ competent cells was obtained from Life Technologies (Grand Island, NY).

    Protein Concentration:

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again. .. Purity of the recombinant proteins was verified on immunoblot using the monoclonal anti-polyHistidine-peroxidase (A7058-1VL: Sigma Aldrich, UK) and protein concentration was measured by the Lowry method (Bio-Rad) following the manufacturer’s protocol.

    Sequencing:

    Article Title: Inflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms
    Article Snippet: Four microliters of the Gibson assembly mix were transformed into chemically competent E. coli Mach1 T1 cells (Life Technologies). .. Constructs were verified using colony PCR, restriction analysis and sequencing.

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: .. The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells. .. Transformants were selected on low-salt Luria-Bertani plates containing 25 μg/mL zeocin (Duchefa Biochemie) and analyzed by direct PCR amplification using specific primers of the α-factor signal peptide and the 3′-Alcohol Oxidase terminator sequence according to the manufacturer’s instruction.

    Article Title: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses
    Article Snippet: .. The resulting plasmid, designated as pTN-HMY (Figure ), was then transformed into chemically competent E. coli Top10 cells (Invitrogen) and inoculated onto LB agar plates supplemented with tetracycline and ampicillin and sequence confirmed. ..

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: We used the Advantage 2 PCR kit from Clontech (Mountain View, USA) to amplify the coding sequence from TL zebrafish liver cDNA with 1 μM of each primer; the thermal profile was: 94°C for 1 min, [94°C for 30 sec, 58°C for 3 min] for 35 cycles, and 68°C for 7 min. PCR products were purified with the QIAquick Gel Extraction Kit from Qiagen (Valencia, USA) and cloned into pGEM-T Easy from Promega (Madison, USA) as per kit instructions. .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA).

    Article Title: Genome-Wide Transposon Mutagenesis of Borrelia burgdorferi for Identification of Phenotypic Mutants
    Article Snippet: Transposon insertions along with B. burgdorferi flanking DNA were recovered by transforming 5 μl of the ligation reaction mixture into chemically competent E. coli TOP10 cells (Invitrogen). .. Primers Col (5′-CAGCAACGCGGCCTTTTTACG) and Flg (5′-TTTTTTGTTTGTTTTAAAAT) were used to sequence out from the transposon into the flanking B. burgdorferi DNA (Fig. ).

    Recombinant:

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Paragraph title: Recombinant proteins preparation ... Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen).

    Article Title: Development of novel O-polysaccharide based glycoconjugates for immunization against glanders
    Article Snippet: Paragraph title: Recombinant DNA techniques ... Chemically competent E. coli TOP10 cells were transformed as per the manufacturer's instructions (Invitrogen).

    Article Title: Antioxidant Efficacy and Adhesion Rescue by a Recombinant Mussel Foot Protein-6
    Article Snippet: .. E. coli One Shot TOP10 chemically competent cells [ F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 Δlacχ74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG λ- ] (Life Technologies Grand Island, NY) were used for recombinant plasmid construction. .. E. coli Rosetta 2 (DE3) cells [F− ompT hsdS B (rB − mB − ) gal dcm (DE3) pRARE (CamR )] were used as a host strain for expressing recombinant rmfp-6.1.

    Mutagenesis:

    Article Title: A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica
    Article Snippet: Chemically competent E. coli Mach1TM cells (Life Technologies, Inc., Grand Island, NY) were used for all cloning procedures. .. The following antibiotics were added to media as required for selection: ampicillin, 100 μg/mL; streptomycin, 50 μg/mL; chloramphenicol, 20 μg/mL; kanamycin, 50 μg/mL when the kan resistance gene was present on a high-copy-number plasmid and for selection after integration of the mutation cassette into the target DNA, and 20 μg/mL for subsequent experiments after the kan resistance gene had been integrated into the genomic DNA; trimethoprim, 50 μg/mL when the resistance gene was present on a high copy-number plasmid or 25 μg/mL when the resistance gene was integrated into the genomic DNA.

    Article Title: The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae
    Article Snippet: Paragraph title: Allelic exchange mutagenesis of the pdi locus. ... This vector was then used to transform chemically competent Mach 1 E. coli cells (Invitrogen).

    Isolation:

    Article Title: Isolation of Highly Active Monoclonal Antibodies against Multiresistant Gram-Positive Bacteria
    Article Snippet: The ligation reaction mixture was transformed into competent E. coli TOP10 cells (Invitrogen) and plasmids were purified using a plasmid Miniprep kit (QIAGEN). .. Plasmids were transformed into E. coli TOP10 cells (Invitrogen) , individual colonies were isolated, plasmids were obtained, and the inserted DNA was sequenced to ensure that the correct L chain V-region was cloned into the eukaryotic expression vector.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). .. Plasmids were isolated from the bacteria, and transfected into E . coli BL21 (DE3) gold (Agilent) for expression.

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA). .. Three clones were sent to Eurofins MWG Operon (Louisville, USA) for forward and reverse sequencing with T7 primers (1 μg of plasmid DNA), and the full-length sequence was then assembled and aligned with [GenBank: ] using MacVector 12.0.2 (Oxford Molecular Group, Madison, USA).

    Article Title: Genome-Wide Transposon Mutagenesis of Borrelia burgdorferi for Identification of Phenotypic Mutants
    Article Snippet: Transposon insertions along with B. burgdorferi flanking DNA were recovered by transforming 5 μl of the ligation reaction mixture into chemically competent E. coli TOP10 cells (Invitrogen). .. Individual E. coli transformants were inoculated into 5-ml cultures, and plasmid DNA was isolated using the Qiaprep Spin Miniprep kit (QIAGEN, Valencia, Calif.).

    Size-exclusion Chromatography:

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: We used the Advantage 2 PCR kit from Clontech (Mountain View, USA) to amplify the coding sequence from TL zebrafish liver cDNA with 1 μM of each primer; the thermal profile was: 94°C for 1 min, [94°C for 30 sec, 58°C for 3 min] for 35 cycles, and 68°C for 7 min. PCR products were purified with the QIAquick Gel Extraction Kit from Qiagen (Valencia, USA) and cloned into pGEM-T Easy from Promega (Madison, USA) as per kit instructions. .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA).

    Labeling:

    Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
    Article Snippet: Wildtype chicken avidin (Belovo, Belgium, MW = 16 kDa/monomer) and fibronectin (gelatin-affinity purified from human serum, MW = 220 kDa/monomer) had been previously labeled with Alexa Fluor® 488 NHS ester (Cat. No. A-20000, Thermo Fisher Scientific, Inc. Waltham, MA, USA) by Dr. J. Pärssinen according to manufacturer’s instructions. .. Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and biotinylated alkaline phosphatase (Cat. No. B-2005, Vector Laboratories (Burlingame, CA, USA) were used.

    Purification:

    Article Title: Isolation of Highly Active Monoclonal Antibodies against Multiresistant Gram-Positive Bacteria
    Article Snippet: .. The ligation reaction mixture was transformed into competent E. coli TOP10 cells (Invitrogen) and plasmids were purified using a plasmid Miniprep kit (QIAGEN). .. For light (L) chains, variable domains of the light chain cloned into the TOPO cloning vector 2.1 were digested with BglII and AvrII restriction enzymes (NEB) and ligated with the TCAE6.7 vector already containing the matching H chain variable region and cut with the same enzymes.

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: Paragraph title: FpPG Expression and Purification ... The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again. .. E . coli lysates were prepared under denaturing conditions and His-tagged proteins (with six histidins) were purified under denaturing conditions with 8M urea in Ni-NTA column (731–1550: Bio-Rad, USA).

    Article Title: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses
    Article Snippet: The resulting plasmid, designated as pTN-HMY (Figure ), was then transformed into chemically competent E. coli Top10 cells (Invitrogen) and inoculated onto LB agar plates supplemented with tetracycline and ampicillin and sequence confirmed. .. The 6.9 kb was purified from the agarose gel, and mixed with EZ-Tn5 transposase (Epicentre).

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: We used the Advantage 2 PCR kit from Clontech (Mountain View, USA) to amplify the coding sequence from TL zebrafish liver cDNA with 1 μM of each primer; the thermal profile was: 94°C for 1 min, [94°C for 30 sec, 58°C for 3 min] for 35 cycles, and 68°C for 7 min. PCR products were purified with the QIAquick Gel Extraction Kit from Qiagen (Valencia, USA) and cloned into pGEM-T Easy from Promega (Madison, USA) as per kit instructions. .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA).

    Article Title: Development of novel O-polysaccharide based glycoconjugates for immunization against glanders
    Article Snippet: Genomic DNA was purified using a Wizard Genomic DNA Purification kit (Promega). .. Chemically competent E. coli TOP10 cells were transformed as per the manufacturer's instructions (Invitrogen).

    Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
    Article Snippet: Wildtype chicken avidin (Belovo, Belgium, MW = 16 kDa/monomer) and fibronectin (gelatin-affinity purified from human serum, MW = 220 kDa/monomer) had been previously labeled with Alexa Fluor® 488 NHS ester (Cat. No. A-20000, Thermo Fisher Scientific, Inc. Waltham, MA, USA) by Dr. J. Pärssinen according to manufacturer’s instructions. .. Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and biotinylated alkaline phosphatase (Cat. No. B-2005, Vector Laboratories (Burlingame, CA, USA) were used.

    Polymerase Chain Reaction:

    Article Title: Inflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms
    Article Snippet: The pWSK29 PCR fragment was combined with the cirA or cib /imm fragment in a Gibson assembly reaction . .. Four microliters of the Gibson assembly mix were transformed into chemically competent E. coli Mach1 T1 cells (Life Technologies).

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: .. Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). ..

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells. .. Transformants were selected on low-salt Luria-Bertani plates containing 25 μg/mL zeocin (Duchefa Biochemie) and analyzed by direct PCR amplification using specific primers of the α-factor signal peptide and the 3′-Alcohol Oxidase terminator sequence according to the manufacturer’s instruction.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again.

    Article Title: The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae
    Article Snippet: The fusion product resulting from this PCR contained an in-frame substitution of pdi with cat and was subcloned into the Gateway entry vector pCR8/GW/TOPO. .. This vector was then used to transform chemically competent Mach 1 E. coli cells (Invitrogen).

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: We used the Advantage 2 PCR kit from Clontech (Mountain View, USA) to amplify the coding sequence from TL zebrafish liver cDNA with 1 μM of each primer; the thermal profile was: 94°C for 1 min, [94°C for 30 sec, 58°C for 3 min] for 35 cycles, and 68°C for 7 min. PCR products were purified with the QIAquick Gel Extraction Kit from Qiagen (Valencia, USA) and cloned into pGEM-T Easy from Promega (Madison, USA) as per kit instructions. .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA).

    Article Title: Development of novel O-polysaccharide based glycoconjugates for immunization against glanders
    Article Snippet: PCR and restriction digested products were purified using a QIAquick Gel Extraction Kit (Qiagen). .. Chemically competent E. coli TOP10 cells were transformed as per the manufacturer's instructions (Invitrogen).

    Positron Emission Tomography:

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again. .. Plasmids were isolated from the bacteria, and transfected into E . coli BL21 (DE3) gold (Agilent) for expression.

    Microscopy:

    Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
    Article Snippet: Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and biotinylated alkaline phosphatase (Cat. No. B-2005, Vector Laboratories (Burlingame, CA, USA) were used. .. Vectashield Hardset Antifade Mounting Medium (Cat. No. H-1400, Vector Laboratories) was used for microscope sample preparation.

    IA:

    Article Title: Development of novel O-polysaccharide based glycoconjugates for immunization against glanders
    Article Snippet: Chemically competent E. coli TOP10 cells were transformed as per the manufacturer's instructions (Invitrogen). .. Oligonucleotide primers were obtained from Integrated DNA Technologies (Coralville, IA).

    Agarose Gel Electrophoresis:

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells. .. Plasmid DNA was extracted from the cells using a plasmid mini prep kit (Macherey & Nagel) and analyzed by digestion with Eco RI and Xba I restriction enzymes, followed by 1% agarose gel analysis.

    Article Title: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses
    Article Snippet: The resulting plasmid, designated as pTN-HMY (Figure ), was then transformed into chemically competent E. coli Top10 cells (Invitrogen) and inoculated onto LB agar plates supplemented with tetracycline and ampicillin and sequence confirmed. .. The 6.9 kb was purified from the agarose gel, and mixed with EZ-Tn5 transposase (Epicentre).

    Plasmid Preparation:

    Article Title: Inflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms
    Article Snippet: Primers Cib-Imm-pWSK29-Gbs-for and Cib-Imm-pWSK29-Gbs-rev were used in a PCR with S. Typhimurium strain SL1344 plasmid pCol1B9_SL1344 as a template to amplify the cib /imm locus including both natural promoters. .. Four microliters of the Gibson assembly mix were transformed into chemically competent E. coli Mach1 T1 cells (Life Technologies).

    Article Title: Isolation of Highly Active Monoclonal Antibodies against Multiresistant Gram-Positive Bacteria
    Article Snippet: .. The ligation reaction mixture was transformed into competent E. coli TOP10 cells (Invitrogen) and plasmids were purified using a plasmid Miniprep kit (QIAGEN). .. For light (L) chains, variable domains of the light chain cloned into the TOPO cloning vector 2.1 were digested with BglII and AvrII restriction enzymes (NEB) and ligated with the TCAE6.7 vector already containing the matching H chain variable region and cut with the same enzymes.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: .. Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). ..

    Article Title: Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1Structural Resolution of the Complex between a Fungal Polygalacturonase and a Plant Polygalacturonase-Inhibiting Protein by Small-Angle X-Ray Scattering 1 [W]
    Article Snippet: The construct, generated in frame with the signal sequence for secretion of the yeast ( Saccharomyces cerevisiae ) α factor, was amplified by transforming Escherichia coli DH5α [genotype: F- φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 deo R rec A1 end A1 hsd R17(rk−, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ-, provided by Invitrogen] competent cells. .. Plasmid DNA was extracted from the cells using a plasmid mini prep kit (Macherey & Nagel) and analyzed by digestion with Eco RI and Xba I restriction enzymes, followed by 1% agarose gel analysis.

    Article Title: Recombinant Salivary Proteins of Phlebotomus orientalis are Suitable Antigens to Measure Exposure of Domestic Animals to Sand Fly Bites
    Article Snippet: Briefly, PCR products were ligated into E . coli pGEM-T Easy Vector (Promega) using TA cloning and the ligation products were transfected into E . coli competent cells TOP10 (Invitrogen). .. Restricted E . coli pET-42 Expression Vectors (Novagen) were ligated and ligation products were transformed into E . coli competent cells TOP10 (Invitrogen) again.

    Article Title: A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica
    Article Snippet: Chemically competent E. coli Mach1TM cells (Life Technologies, Inc., Grand Island, NY) were used for all cloning procedures. .. The following antibiotics were added to media as required for selection: ampicillin, 100 μg/mL; streptomycin, 50 μg/mL; chloramphenicol, 20 μg/mL; kanamycin, 50 μg/mL when the kan resistance gene was present on a high-copy-number plasmid and for selection after integration of the mutation cassette into the target DNA, and 20 μg/mL for subsequent experiments after the kan resistance gene had been integrated into the genomic DNA; trimethoprim, 50 μg/mL when the resistance gene was present on a high copy-number plasmid or 25 μg/mL when the resistance gene was integrated into the genomic DNA.

    Article Title: The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae
    Article Snippet: .. This vector was then used to transform chemically competent Mach 1 E. coli cells (Invitrogen). .. Plasmid DNA was extracted, and the fusion PCR amplicon was transferred into the temperature-sensitive knockout vector pKODestErm ( ) via an attL–attR (LR) recombination reaction to create the knockout plasmid pKOpdi.

    Article Title: Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses
    Article Snippet: .. The resulting plasmid, designated as pTN-HMY (Figure ), was then transformed into chemically competent E. coli Top10 cells (Invitrogen) and inoculated onto LB agar plates supplemented with tetracycline and ampicillin and sequence confirmed. ..

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA). .. Three clones were sent to Eurofins MWG Operon (Louisville, USA) for forward and reverse sequencing with T7 primers (1 μg of plasmid DNA), and the full-length sequence was then assembled and aligned with [GenBank: ] using MacVector 12.0.2 (Oxford Molecular Group, Madison, USA).

    Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
    Article Snippet: Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and biotinylated alkaline phosphatase (Cat. No. B-2005, Vector Laboratories (Burlingame, CA, USA) were used. .. Vectashield Hardset Antifade Mounting Medium (Cat. No. H-1400, Vector Laboratories) was used for microscope sample preparation.

    Article Title: Antioxidant Efficacy and Adhesion Rescue by a Recombinant Mussel Foot Protein-6
    Article Snippet: .. E. coli One Shot TOP10 chemically competent cells [ F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 Δlacχ74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG λ- ] (Life Technologies Grand Island, NY) were used for recombinant plasmid construction. .. E. coli Rosetta 2 (DE3) cells [F− ompT hsdS B (rB − mB − ) gal dcm (DE3) pRARE (CamR )] were used as a host strain for expressing recombinant rmfp-6.1.

    Article Title: Genome-Wide Transposon Mutagenesis of Borrelia burgdorferi for Identification of Phenotypic Mutants
    Article Snippet: Vector pMarGent was constructed such that the transposon and B. burgdorferi DNA flanking the insertion site could be rescued easily in E. coli (Fig. and ). .. Transposon insertions along with B. burgdorferi flanking DNA were recovered by transforming 5 μl of the ligation reaction mixture into chemically competent E. coli TOP10 cells (Invitrogen).

    Software:

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA). .. Secondary structure prediction Amino acid sequences of CYP20A1 proteins were retrieved from the Ensembl database ( ) and aligned with MacVector 12.0.2 software (Oxford Molecular Group, USA) (see for sequence identifiers).

    Avidin-Biotin Assay:

    Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
    Article Snippet: .. Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and biotinylated alkaline phosphatase (Cat. No. B-2005, Vector Laboratories (Burlingame, CA, USA) were used. .. Vectashield Hardset Antifade Mounting Medium (Cat. No. H-1400, Vector Laboratories) was used for microscope sample preparation.

    Selection:

    Article Title: A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica
    Article Snippet: Chemically competent E. coli Mach1TM cells (Life Technologies, Inc., Grand Island, NY) were used for all cloning procedures. .. The following antibiotics were added to media as required for selection: ampicillin, 100 μg/mL; streptomycin, 50 μg/mL; chloramphenicol, 20 μg/mL; kanamycin, 50 μg/mL when the kan resistance gene was present on a high-copy-number plasmid and for selection after integration of the mutation cassette into the target DNA, and 20 μg/mL for subsequent experiments after the kan resistance gene had been integrated into the genomic DNA; trimethoprim, 50 μg/mL when the resistance gene was present on a high copy-number plasmid or 25 μg/mL when the resistance gene was integrated into the genomic DNA.

    Article Title: The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae
    Article Snippet: This vector was then used to transform chemically competent Mach 1 E. coli cells (Invitrogen). .. Transformants were identified at 30 °C by Erm selection and shifted to 37 °C (a non-permissive temperature for plasmid replication).

    Sample Prep:

    Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
    Article Snippet: Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and biotinylated alkaline phosphatase (Cat. No. B-2005, Vector Laboratories (Burlingame, CA, USA) were used. .. Vectashield Hardset Antifade Mounting Medium (Cat. No. H-1400, Vector Laboratories) was used for microscope sample preparation.

    Functional Assay:

    Article Title: Antioxidant Efficacy and Adhesion Rescue by a Recombinant Mussel Foot Protein-6
    Article Snippet: E. coli One Shot TOP10 chemically competent cells [ F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 Δlacχ74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG λ- ] (Life Technologies Grand Island, NY) were used for recombinant plasmid construction. .. Previous studies on the heterologous expression of recombinant mussel adhesive proteins frequently led to low yields or failed to express functional protein in E. coli BL21 or JM109 strains (personal communication, Dr. Dong-Soo Hwang).

    Knock-Out:

    Article Title: The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae
    Article Snippet: This vector was then used to transform chemically competent Mach 1 E. coli cells (Invitrogen). .. Plasmid DNA was extracted, and the fusion PCR amplicon was transferred into the temperature-sensitive knockout vector pKODestErm ( ) via an attL–attR (LR) recombination reaction to create the knockout plasmid pKOpdi.

    Produced:

    Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
    Article Snippet: Materials Laser-cut electrochemically polished 12.5 mm × 28 mm × 0.7 mm EN 1.4404 (AISI 316L) SS chips produced by Outokumpu Stainless Oy (Tornio, Finland) were ordered from Kaarinan Trimet Oy ( http://www.kaarinantrimet.fi/ ). .. Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and biotinylated alkaline phosphatase (Cat. No. B-2005, Vector Laboratories (Burlingame, CA, USA) were used.

    DNA Purification:

    Article Title: Development of novel O-polysaccharide based glycoconjugates for immunization against glanders
    Article Snippet: Genomic DNA was purified using a Wizard Genomic DNA Purification kit (Promega). .. Chemically competent E. coli TOP10 cells were transformed as per the manufacturer's instructions (Invitrogen).

    Marker:

    Article Title: Genome-Wide Transposon Mutagenesis of Borrelia burgdorferi for Identification of Phenotypic Mutants
    Article Snippet: Only circularized DNA containing the ColE1 origin of replication and the gentamicin resistance marker (carried on the transposon) would successfully transform E. coli . .. Transposon insertions along with B. burgdorferi flanking DNA were recovered by transforming 5 μl of the ligation reaction mixture into chemically competent E. coli TOP10 cells (Invitrogen).

    Gel Extraction:

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: We used the Advantage 2 PCR kit from Clontech (Mountain View, USA) to amplify the coding sequence from TL zebrafish liver cDNA with 1 μM of each primer; the thermal profile was: 94°C for 1 min, [94°C for 30 sec, 58°C for 3 min] for 35 cycles, and 68°C for 7 min. PCR products were purified with the QIAquick Gel Extraction Kit from Qiagen (Valencia, USA) and cloned into pGEM-T Easy from Promega (Madison, USA) as per kit instructions. .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA).

    Article Title: Development of novel O-polysaccharide based glycoconjugates for immunization against glanders
    Article Snippet: PCR and restriction digested products were purified using a QIAquick Gel Extraction Kit (Qiagen). .. Chemically competent E. coli TOP10 cells were transformed as per the manufacturer's instructions (Invitrogen).

    Fluorescence In Situ Hybridization:

    Article Title: Cytochrome P450 20A1 in zebrafish: Cloning, regulation and potential involvement in hyperactivity disorders
    Article Snippet: Fish maintenance and breeding procedures were previously described ( ; ). cDNA Cloning and Sequencing Primers were designed in the proximal 5’ and 3’ untranslated regions of zebrafish CYP20A1 transcript [GenBank: ]. .. Mach-1 competent cells from Invitrogen (Carlsbad, USA) were transformed, and, following overnight incubation of selected ampicillin-resistant white colonies in liquid Luria-Bertani medium with antibiotic, plasmids were isolated with the QIAprep Spin Miniprep Kit from Qiagen (Valencia, USA).

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    Thermo Fisher one shot top10 chemically competent e coli
    One Shot Top10 Chemically Competent E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shot top10 chemically competent e coli/product/Thermo Fisher
    Average 90 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    one shot top10 chemically competent e coli - by Bioz Stars, 2020-01
    90/100 stars
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    MultiShot StripWell TOP10 chemically competent E coli cells are cloning competent cells that packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium throughput bacterial
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