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Merck KGaA competent novablue e coli cells
Competent Novablue E Coli Cells, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/competent novablue e coli cells/product/Merck KGaA
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
competent novablue e coli cells - by Bioz Stars, 2020-04
86/100 stars

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Clone Assay:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: Half of the cells were then spread on LB agar containing 32 μg/ml Amp and incubated at 37°C for a minimum of 16 h. The bla gene sequence and the Amp MIC for clones producing active TEM-1 variants were determined as described subsequently. .. With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA).

DNA Ligation:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA). .. The DNA was desalted and 100 ng of the DNA ligated using the Fast-Link™ DNA ligation kit (Epicentre Biotechnologies) to recircularize pNOM.

Ligation:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: .. With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA). .. To calculate the number of transformed cells containing the pNOM-SubSeqNNN plasmid, the equivalent of 1/30 of the transformation mix post-recovery was grown on LB agar plates containing 25 μg/ml Kan and the number of colony forming units (c.f.u.) observed after incubation at 37°C overnight was determined (routinely generated ca 4 × 105 c.f.u./μg DNA).

Mutagenesis:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: The bla gene prior to SubSeq removal was also sequenced for several inactive variants to predict the mutation that resulted in loss of β-lactamase activity. .. With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA).

Isolation:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA). .. The remainder of the transformation culture was added to 50 ml of LB broth containing 200 μg/ml Kan and incubated at 37°C for 16 h. Plasmid DNA was isolated from the culture using the QIAprep Spin Miniprep kit (Qiagen), and 2 μg of this DNA was digested with MlyI to remove the SubSeq section.

Incubation:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: Half of the cells were then spread on LB agar containing 32 μg/ml Amp and incubated at 37°C for a minimum of 16 h. The bla gene sequence and the Amp MIC for clones producing active TEM-1 variants were determined as described subsequently. .. With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA).

Generated:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA). .. To calculate the number of transformed cells containing the pNOM-SubSeqNNN plasmid, the equivalent of 1/30 of the transformation mix post-recovery was grown on LB agar plates containing 25 μg/ml Kan and the number of colony forming units (c.f.u.) observed after incubation at 37°C overnight was determined (routinely generated ca 4 × 105 c.f.u./μg DNA).

Activity Assay:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: The bla gene prior to SubSeq removal was also sequenced for several inactive variants to predict the mutation that resulted in loss of β-lactamase activity. .. With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA).

Transmission Electron Microscopy:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: Half of the cells were then spread on LB agar containing 32 μg/ml Amp and incubated at 37°C for a minimum of 16 h. The bla gene sequence and the Amp MIC for clones producing active TEM-1 variants were determined as described subsequently. .. With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA).

Sequencing:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: Half of the cells were then spread on LB agar containing 32 μg/ml Amp and incubated at 37°C for a minimum of 16 h. The bla gene sequence and the Amp MIC for clones producing active TEM-1 variants were determined as described subsequently. .. With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA).

Transformation Assay:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA). .. To calculate the number of transformed cells containing the pNOM-SubSeqNNN plasmid, the equivalent of 1/30 of the transformation mix post-recovery was grown on LB agar plates containing 25 μg/ml Kan and the number of colony forming units (c.f.u.) observed after incubation at 37°C overnight was determined (routinely generated ca 4 × 105 c.f.u./μg DNA).

Plasmid Preparation:

Article Title: Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Article Snippet: The plasmid (ca 20 ng) was recircularized directly after digestion using T4 DNA ligase (Promega UK, Southampton, UK). .. With regards to the BLAΔ1644 library, the ligation reaction to insert SubSeqNNN within linearized pNOM was then used to transform (ca 15 ng of DNA/transformation) high efficiency chemically competent NovaBlue E. coli cells (Merck KGaA).

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    Merck KGaA competent novablue e coli cells
    Competent Novablue E Coli Cells, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent novablue e coli cells/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    competent novablue e coli cells - by Bioz Stars, 2020-04
    86/100 stars
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