competent escherichia coli top10 cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher competent escherichia coli top10 cells
    Competent Escherichia Coli Top10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent escherichia coli top10 cells/product/Thermo Fisher
    Average 99 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    competent escherichia coli top10 cells - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Crystallographic Insights into the Pore Structures and Mechanisms of the EutL and EutM Shell Proteins of the Ethanolamine-Utilizing Microcompartment of Escherichia coli ▿ ▿ †
    Article Snippet: .. The resulting products were cloned into a pET101 vector (Invitrogen) according to the company's protocol and were subsequently transformed into chemically competent TOP10 cells (Invitrogen). .. Plasmids of clones that were positively identified by PCR were transformed into BL21-AI cells for overexpression.

    Article Title: A Diverse Range of Bacterial and Eukaryotic Chitinases Hydrolyzes the LacNAc (Galβ1–4GlcNAc) and LacdiNAc (GalNAcβ1–4GlcNAc) Motifs Found on Vertebrate and Insect Cells *
    Article Snippet: .. The synthetic genes were cloned into pET15b expression vectors, which were transformed into chemically competent E. coli TOP10 cells (Invitrogen) for long term storage. .. For protein expression the vector carrying SG1474 was transformed into E. coli BL21(DE3) cells (Novagen, Darmstadt, Germany), whereas the vector carrying was transformed into E. coli BL21/TUNERTM (DE3) cells (Novagen).

    Ligation:

    Article Title: Generation of Human PTH1R Construct with Flag Epitope Located Internally: Comparison of Two-Fragment Assembly by Using PCR Overlap Extension or Ligase
    Article Snippet: .. The ligation mixture was then transformed to competent cells (Invitrogen) and screened by ampicillin. ..

    Article Title: An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening *
    Article Snippet: .. After an inactivation step at 70 °C for 5 min to inactivate the T4 polynucleotide kinase, the PCR products were circularized using 350 U T4 DNA ligase (Takara) at 12 °C for 16 h. 10 μl of the ligation mixture was then used to transform competent E. coli Top10 (Invitrogen) and the transformation mixture was plated on a Luria-Bertani (LB) agar plate containing 100 μg/ml ampicillin and incubated at 37 °C. .. Ten colonies were picked up randomly and the plasmid DNA was extracted and digested by the designed restriction enzyme Xho I along with another enzyme Bgl II.

    Construct:

    Article Title: The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol
    Article Snippet: .. This construct was transformed into TOP10 competent cells (Invitrogen) for expression. ..

    Incubation:

    Article Title: What Goes in Must Come out: Testing for Biases in Molecular Analysis of Arbuscular Mycorrhizal Fungal Communities
    Article Snippet: .. Vectors (4µl) were transformed into Escherichia coli cells by incubation with 25µl competent E. coli cells (DH5α; Invitrogen, Renfrewshire, UK) at 4°C for 30 minutes, followed by a heatshock of 42°C for 45 seconds and rotary incubation with 475µl Super Optimal broth with Catabolite repression (SOC) at 37°C for 1 hour. ..

    Article Title: An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening *
    Article Snippet: .. After an inactivation step at 70 °C for 5 min to inactivate the T4 polynucleotide kinase, the PCR products were circularized using 350 U T4 DNA ligase (Takara) at 12 °C for 16 h. 10 μl of the ligation mixture was then used to transform competent E. coli Top10 (Invitrogen) and the transformation mixture was plated on a Luria-Bertani (LB) agar plate containing 100 μg/ml ampicillin and incubated at 37 °C. .. Ten colonies were picked up randomly and the plasmid DNA was extracted and digested by the designed restriction enzyme Xho I along with another enzyme Bgl II.

    other:

    Article Title: Ex vivo tools for the clonal analysis of zebrafish hematopoiesis
    Article Snippet: BL21-CodonPlus (DE3)-RIL competent cells (Agilent Technologies, cat. no. 230245-41) LB broth, Miller (Sigma-Aldrich, cat. no. L2542) sf21 insect cells (Gibco, cat. no. 11497-013) Sf-900 II SFM insect medium (Gibco, cat. no. 10902-096) Top10 competent cells (Invitrogen, cat. no. C4040-10)

    Expressing:

    Article Title: The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol
    Article Snippet: .. This construct was transformed into TOP10 competent cells (Invitrogen) for expression. ..

    Article Title: A Diverse Range of Bacterial and Eukaryotic Chitinases Hydrolyzes the LacNAc (Galβ1–4GlcNAc) and LacdiNAc (GalNAcβ1–4GlcNAc) Motifs Found on Vertebrate and Insect Cells *
    Article Snippet: .. The synthetic genes were cloned into pET15b expression vectors, which were transformed into chemically competent E. coli TOP10 cells (Invitrogen) for long term storage. .. For protein expression the vector carrying SG1474 was transformed into E. coli BL21(DE3) cells (Novagen, Darmstadt, Germany), whereas the vector carrying was transformed into E. coli BL21/TUNERTM (DE3) cells (Novagen).

    Polymerase Chain Reaction:

    Article Title: An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening *
    Article Snippet: .. After an inactivation step at 70 °C for 5 min to inactivate the T4 polynucleotide kinase, the PCR products were circularized using 350 U T4 DNA ligase (Takara) at 12 °C for 16 h. 10 μl of the ligation mixture was then used to transform competent E. coli Top10 (Invitrogen) and the transformation mixture was plated on a Luria-Bertani (LB) agar plate containing 100 μg/ml ampicillin and incubated at 37 °C. .. Ten colonies were picked up randomly and the plasmid DNA was extracted and digested by the designed restriction enzyme Xho I along with another enzyme Bgl II.

    Transformation Assay:

    Article Title: Generation of Human PTH1R Construct with Flag Epitope Located Internally: Comparison of Two-Fragment Assembly by Using PCR Overlap Extension or Ligase
    Article Snippet: .. The ligation mixture was then transformed to competent cells (Invitrogen) and screened by ampicillin. ..

    Article Title: Crystallographic Insights into the Pore Structures and Mechanisms of the EutL and EutM Shell Proteins of the Ethanolamine-Utilizing Microcompartment of Escherichia coli ▿ ▿ †
    Article Snippet: .. The resulting products were cloned into a pET101 vector (Invitrogen) according to the company's protocol and were subsequently transformed into chemically competent TOP10 cells (Invitrogen). .. Plasmids of clones that were positively identified by PCR were transformed into BL21-AI cells for overexpression.

    Article Title: What Goes in Must Come out: Testing for Biases in Molecular Analysis of Arbuscular Mycorrhizal Fungal Communities
    Article Snippet: .. Vectors (4µl) were transformed into Escherichia coli cells by incubation with 25µl competent E. coli cells (DH5α; Invitrogen, Renfrewshire, UK) at 4°C for 30 minutes, followed by a heatshock of 42°C for 45 seconds and rotary incubation with 475µl Super Optimal broth with Catabolite repression (SOC) at 37°C for 1 hour. ..

    Article Title: The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol
    Article Snippet: .. This construct was transformed into TOP10 competent cells (Invitrogen) for expression. ..

    Article Title: An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening *
    Article Snippet: .. After an inactivation step at 70 °C for 5 min to inactivate the T4 polynucleotide kinase, the PCR products were circularized using 350 U T4 DNA ligase (Takara) at 12 °C for 16 h. 10 μl of the ligation mixture was then used to transform competent E. coli Top10 (Invitrogen) and the transformation mixture was plated on a Luria-Bertani (LB) agar plate containing 100 μg/ml ampicillin and incubated at 37 °C. .. Ten colonies were picked up randomly and the plasmid DNA was extracted and digested by the designed restriction enzyme Xho I along with another enzyme Bgl II.

    Article Title: A Diverse Range of Bacterial and Eukaryotic Chitinases Hydrolyzes the LacNAc (Galβ1–4GlcNAc) and LacdiNAc (GalNAcβ1–4GlcNAc) Motifs Found on Vertebrate and Insect Cells *
    Article Snippet: .. The synthetic genes were cloned into pET15b expression vectors, which were transformed into chemically competent E. coli TOP10 cells (Invitrogen) for long term storage. .. For protein expression the vector carrying SG1474 was transformed into E. coli BL21(DE3) cells (Novagen, Darmstadt, Germany), whereas the vector carrying was transformed into E. coli BL21/TUNERTM (DE3) cells (Novagen).

    Plasmid Preparation:

    Article Title: Crystallographic Insights into the Pore Structures and Mechanisms of the EutL and EutM Shell Proteins of the Ethanolamine-Utilizing Microcompartment of Escherichia coli ▿ ▿ †
    Article Snippet: .. The resulting products were cloned into a pET101 vector (Invitrogen) according to the company's protocol and were subsequently transformed into chemically competent TOP10 cells (Invitrogen). .. Plasmids of clones that were positively identified by PCR were transformed into BL21-AI cells for overexpression.

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  • News
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  • 89
    Thermo Fisher competent escherichia coli top10 cells
    Competent Escherichia Coli Top10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent escherichia coli top10 cells/product/Thermo Fisher
    Average 89 stars, based on 73 article reviews
    Price from $9.99 to $1999.99
    competent escherichia coli top10 cells - by Bioz Stars, 2020-08
    89/100 stars
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