competent escherichia coli cells  (TaKaRa)

 
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    Name:
    Stellar Competent Cells
    Description:
    Our Stellar Competent Cells can be used in a wide variety of applications from preparation of cDNA and genomic libraries to construction of longer length genomic libraries subcloning and even methylated DNA cloning This E coli HST08 strain provides high transformation efficiency and since these cells lack the gene cluster for cutting foreign methylated DNA mrr hsdRMS mcrBC and mcrA they are very useful for cloning methylated DNA The cells can also be used for blue white screening i e alpha complementation when transformed with vectors containing the lacZ alpha gene
    Catalog Number:
    636766
    Price:
    None
    Size:
    50 x 100 uL
    Category:
    Stellar chemically competent cells Competent cells Cloning
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    Structured Review

    TaKaRa competent escherichia coli cells
    Our Stellar Competent Cells can be used in a wide variety of applications from preparation of cDNA and genomic libraries to construction of longer length genomic libraries subcloning and even methylated DNA cloning This E coli HST08 strain provides high transformation efficiency and since these cells lack the gene cluster for cutting foreign methylated DNA mrr hsdRMS mcrBC and mcrA they are very useful for cloning methylated DNA The cells can also be used for blue white screening i e alpha complementation when transformed with vectors containing the lacZ alpha gene
    https://www.bioz.com/result/competent escherichia coli cells/product/TaKaRa
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    competent escherichia coli cells - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Molecular Cloning:

    Article Title: Nucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei
    Article Snippet: .. 636763,Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France) were used for plasmid construction and amplification using standard molecular cloning techniques (Sambrook and Russell, ). .. Generation of gene replacement cassettes To express fluorescently labeled transcription factors XYR1 and CRE1 from their native loci, we choose a simultaneous knock-out/knock-in strategy.

    Amplification:

    Article Title: Nucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei
    Article Snippet: .. 636763,Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France) were used for plasmid construction and amplification using standard molecular cloning techniques (Sambrook and Russell, ). .. Generation of gene replacement cassettes To express fluorescently labeled transcription factors XYR1 and CRE1 from their native loci, we choose a simultaneous knock-out/knock-in strategy.

    Clone Assay:

    Article Title: Promotion of Axon Growth by the Secreted End of a Transcription Factor
    Article Snippet: .. The CREB3L2 constructs were cloned using Stellar competent cells (Takara, 636766) and purified with the ZymoPure Plasmid Midiprep kit (Zymo Research). .. The S2P construct pCGN-S2P-WT was a gift from Ron Prywes (Addgene, 32957).

    Article Title: A SNARE-Like Protein and Biotin Are Implicated in Soybean Cyst Nematode Virulence
    Article Snippet: .. The resulting PCR product was treated with cloning enhancer, the plasmid was circularized using the In-Fusion HD enzyme premix and was transformed into E . coli Stellar competent cells following the manufacturers protocol (Clontech). .. The resulting plasmid containing HgSLP-1 missing the signal peptide was verified for accuracy by sequencing the nematode gene as described above.

    Article Title: The “speed gene” effect of myostatin arises in Thoroughbred horses due to a promoter proximal SINE insertion
    Article Snippet: .. The In-Fusion cloning was performed as per manufacturers’ recommendations and the plasmid was transformed into StellarTM competent cells (TaKaRa-Clontech) and was grown at 37°C. .. Subsequently, the plasmid DNA was isolated and sequenced using M13 primers ( 5'-TGT AAA ACG ACG GCC AGT-3' (forward), 5'-CAG GAA ACA GCT ATG ACC-3' (reverse)) by MWG eurofins, to ascertain where the transcription start site was on the sequence, as the inserted fragment sequence would begin at the transcription start site, preceded by the universal primer sequence.

    Construct:

    Article Title: Promotion of Axon Growth by the Secreted End of a Transcription Factor
    Article Snippet: .. The CREB3L2 constructs were cloned using Stellar competent cells (Takara, 636766) and purified with the ZymoPure Plasmid Midiprep kit (Zymo Research). .. The S2P construct pCGN-S2P-WT was a gift from Ron Prywes (Addgene, 32957).

    Purification:

    Article Title: Promotion of Axon Growth by the Secreted End of a Transcription Factor
    Article Snippet: .. The CREB3L2 constructs were cloned using Stellar competent cells (Takara, 636766) and purified with the ZymoPure Plasmid Midiprep kit (Zymo Research). .. The S2P construct pCGN-S2P-WT was a gift from Ron Prywes (Addgene, 32957).

    Article Title: Structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity
    Article Snippet: .. Full-length PCR products were gel purified, ligated into pOPINF expression vector and transformed into competent E. coli Stellar strain cells (Clontech Takara). .. HYS point mutants were obtained as gene fragments (Integrated DNA Technologies, Belgium) with the H127 or F128 codons mutated (H127A CAT- > GCA; H127N CAT- > AAC; F128A TTT- > GCT; F128Y TTT- > TAC) and the pOPINF overhangs included at the 3′ and 5′ extremities.

    Expressing:

    Article Title: Structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity
    Article Snippet: .. Full-length PCR products were gel purified, ligated into pOPINF expression vector and transformed into competent E. coli Stellar strain cells (Clontech Takara). .. HYS point mutants were obtained as gene fragments (Integrated DNA Technologies, Belgium) with the H127 or F128 codons mutated (H127A CAT- > GCA; H127N CAT- > AAC; F128A TTT- > GCT; F128Y TTT- > TAC) and the pOPINF overhangs included at the 3′ and 5′ extremities.

    Polymerase Chain Reaction:

    Article Title: A SNARE-Like Protein and Biotin Are Implicated in Soybean Cyst Nematode Virulence
    Article Snippet: .. The resulting PCR product was treated with cloning enhancer, the plasmid was circularized using the In-Fusion HD enzyme premix and was transformed into E . coli Stellar competent cells following the manufacturers protocol (Clontech). .. The resulting plasmid containing HgSLP-1 missing the signal peptide was verified for accuracy by sequencing the nematode gene as described above.

    Article Title: Structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity
    Article Snippet: .. Full-length PCR products were gel purified, ligated into pOPINF expression vector and transformed into competent E. coli Stellar strain cells (Clontech Takara). .. HYS point mutants were obtained as gene fragments (Integrated DNA Technologies, Belgium) with the H127 or F128 codons mutated (H127A CAT- > GCA; H127N CAT- > AAC; F128A TTT- > GCT; F128Y TTT- > TAC) and the pOPINF overhangs included at the 3′ and 5′ extremities.

    Transformation Assay:

    Article Title: Biochemical Characterization of Hypothetical Proteins from Helicobacter pylori
    Article Snippet: .. Recombinant plasmids were transformed into One Shot TOP10 Chemically Competent E. coli (Invitrogen) or Stellar Competent E. coli (Clontech). .. From the transformed E. coli , recombinant plasmids were purified by QuickClean II Plasmid Miniprep Kit (GenScript), and sequenced by GENEWIZ to confirm the identity of the construct.

    Article Title: A SNARE-Like Protein and Biotin Are Implicated in Soybean Cyst Nematode Virulence
    Article Snippet: .. The resulting PCR product was treated with cloning enhancer, the plasmid was circularized using the In-Fusion HD enzyme premix and was transformed into E . coli Stellar competent cells following the manufacturers protocol (Clontech). .. The resulting plasmid containing HgSLP-1 missing the signal peptide was verified for accuracy by sequencing the nematode gene as described above.

    Article Title: The “speed gene” effect of myostatin arises in Thoroughbred horses due to a promoter proximal SINE insertion
    Article Snippet: .. The In-Fusion cloning was performed as per manufacturers’ recommendations and the plasmid was transformed into StellarTM competent cells (TaKaRa-Clontech) and was grown at 37°C. .. Subsequently, the plasmid DNA was isolated and sequenced using M13 primers ( 5'-TGT AAA ACG ACG GCC AGT-3' (forward), 5'-CAG GAA ACA GCT ATG ACC-3' (reverse)) by MWG eurofins, to ascertain where the transcription start site was on the sequence, as the inserted fragment sequence would begin at the transcription start site, preceded by the universal primer sequence.

    Article Title: A pooled single-cell genetic screen identifies regulatory checkpoints in the continuum of the epithelial-to-mesenchymal transition
    Article Snippet: .. Libraries were then transformed into stellar competent cells (Clontech), transformations were diluted in 250 μl of LB, spread onto 6 LB agar plates containing ampicillin and bacteria culture at 30 ˚C for 24 hours. .. Resulting colonies were scraped with LB, pooled and vector recovered using a DNA midi kit (Qiagen).

    Article Title: Unprecedented biomass and fatty acid production by the newly discovered cyanobacterium Synechococcus sp. PCC 11901
    Article Snippet: .. Fragments were then ligated using NEBuilder® HiFi DNA Assembly (New England Biolabs) according to manufacturer’s protocol and transformed to competent E. coli cells (Stellar, TaKaRa). .. A synthetic version of the E. coli thioesterase gene tesA lacking its cognate signal sequence (‘tesA ) was codon-optimized for Syn7002 (by GenScript, Hong Kong, Ltd). pAcsA_cpt_YFP and pAcsA_cLac143_YFP vector templates for PCR were a kind gift from Prof. Brian Pfleger, University of Wisconsin-Madison, USA.

    Article Title: Structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity
    Article Snippet: .. Full-length PCR products were gel purified, ligated into pOPINF expression vector and transformed into competent E. coli Stellar strain cells (Clontech Takara). .. HYS point mutants were obtained as gene fragments (Integrated DNA Technologies, Belgium) with the H127 or F128 codons mutated (H127A CAT- > GCA; H127N CAT- > AAC; F128A TTT- > GCT; F128Y TTT- > TAC) and the pOPINF overhangs included at the 3′ and 5′ extremities.

    Recombinant:

    Article Title: Biochemical Characterization of Hypothetical Proteins from Helicobacter pylori
    Article Snippet: .. Recombinant plasmids were transformed into One Shot TOP10 Chemically Competent E. coli (Invitrogen) or Stellar Competent E. coli (Clontech). .. From the transformed E. coli , recombinant plasmids were purified by QuickClean II Plasmid Miniprep Kit (GenScript), and sequenced by GENEWIZ to confirm the identity of the construct.

    Plasmid Preparation:

    Article Title: Nucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei
    Article Snippet: .. 636763,Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France) were used for plasmid construction and amplification using standard molecular cloning techniques (Sambrook and Russell, ). .. Generation of gene replacement cassettes To express fluorescently labeled transcription factors XYR1 and CRE1 from their native loci, we choose a simultaneous knock-out/knock-in strategy.

    Article Title: Promotion of Axon Growth by the Secreted End of a Transcription Factor
    Article Snippet: .. The CREB3L2 constructs were cloned using Stellar competent cells (Takara, 636766) and purified with the ZymoPure Plasmid Midiprep kit (Zymo Research). .. The S2P construct pCGN-S2P-WT was a gift from Ron Prywes (Addgene, 32957).

    Article Title: A SNARE-Like Protein and Biotin Are Implicated in Soybean Cyst Nematode Virulence
    Article Snippet: .. The resulting PCR product was treated with cloning enhancer, the plasmid was circularized using the In-Fusion HD enzyme premix and was transformed into E . coli Stellar competent cells following the manufacturers protocol (Clontech). .. The resulting plasmid containing HgSLP-1 missing the signal peptide was verified for accuracy by sequencing the nematode gene as described above.

    Article Title: The “speed gene” effect of myostatin arises in Thoroughbred horses due to a promoter proximal SINE insertion
    Article Snippet: .. The In-Fusion cloning was performed as per manufacturers’ recommendations and the plasmid was transformed into StellarTM competent cells (TaKaRa-Clontech) and was grown at 37°C. .. Subsequently, the plasmid DNA was isolated and sequenced using M13 primers ( 5'-TGT AAA ACG ACG GCC AGT-3' (forward), 5'-CAG GAA ACA GCT ATG ACC-3' (reverse)) by MWG eurofins, to ascertain where the transcription start site was on the sequence, as the inserted fragment sequence would begin at the transcription start site, preceded by the universal primer sequence.

    Article Title: Structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity
    Article Snippet: .. Full-length PCR products were gel purified, ligated into pOPINF expression vector and transformed into competent E. coli Stellar strain cells (Clontech Takara). .. HYS point mutants were obtained as gene fragments (Integrated DNA Technologies, Belgium) with the H127 or F128 codons mutated (H127A CAT- > GCA; H127N CAT- > AAC; F128A TTT- > GCT; F128Y TTT- > TAC) and the pOPINF overhangs included at the 3′ and 5′ extremities.

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  • Team
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  • Contact
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  • 92
    TaKaRa competent escherichia coli cells
    Competent Escherichia Coli Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent escherichia coli cells/product/TaKaRa
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    competent escherichia coli cells - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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