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Stratagene competent e coli xl1 blue mrf cells
Competent E Coli Xl1 Blue Mrf Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/competent e coli xl1 blue mrf cells/product/Stratagene
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
competent e coli xl1 blue mrf cells - by Bioz Stars, 2020-04
85/100 stars

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Related Articles

Clone Assay:

Article Title: Detection of Androgenic-Mutagenic Compounds and Potential Autochthonous Bacterial Communities during In Situ Bioremediation of Post-methanated Distillery Sludge
Article Snippet: Paragraph title: Cloning of 16S rDNA PCR Products ... Competent Escherichia coli XL1 blue MRF′ cells (Stratagene) were transformed using the method described by .

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA). .. Colony PCR followed by BclI digestion (the D736A mutation eliminates a BclI restriction site) was performed to identify the mutant clones.

Article Title: Wolbachia Prophage DNA Adenine Methyltransferase Genes in Different Drosophila-Wolbachia Associations
Article Snippet: .. The PCR products were A-tailed, cloned into the pGEM-T Easy vector (Promega, Wisconsin, USA) and transformed into competent E. coli XL1-Blue MRF cells (Stratagene, Amsterdam, The Netherlands). .. Plasmid DNA was extracted using the Nucleospin Plasmid kit (Macherey-Nagel).

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene). .. The library was screened for larger cloned cDNA inserts, which were subsequently sequenced and analyzed with Genetics Computer Group alignment programs with reference to the published CHV1-EP713 nucleotide sequence ( ).

Amplification:

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: Briefly, mutant sense and antisense primers (5′-CACACATTTTCATTCTGCTCATTATGCTGGATTGTC-3′, and 5′-GACAATCCAGCATAATGAGCAGAATGAAAATGTGTG-3′, respectively) were used in PCR amplification, with pAC5.1-hSNM1 as template and Pfu-Turbo DNA polymerase (Stratagene, La Jolla, CA, USA). .. The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA).

Article Title: Wolbachia Prophage DNA Adenine Methyltransferase Genes in Different Drosophila-Wolbachia Associations
Article Snippet: To generate DNA templates for sequencing, PCR reactions were done using the Elongase Amplification System (Invitrogen, Glasgow, UK). .. The PCR products were A-tailed, cloned into the pGEM-T Easy vector (Promega, Wisconsin, USA) and transformed into competent E. coli XL1-Blue MRF cells (Stratagene, Amsterdam, The Netherlands).

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: Based on the prediction that CHV1-Euro7 RNA may have nucleotide sequence similarity with CHV1-EP713 RNA, first-strand CHV1-Euro7 cDNA synthesized with oligo(dT) as a primer was used as a template for PCR amplification of the terminal domains with primer pairs specific for the 5′ terminus (primers RSDS10 and BR18 [CHV1-EP713 map positions 1 to 22 and 350 to 369, respectively] [ ]) and the 3′ terminus (primers BH23 and RSDS11 [CHV1-EP713 map positions 12131 to 12148 and 12677 to 12697, respectively] [ ]). .. The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene).

Synthesized:

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: A cDNA library was synthesized with CHV1-Euro7 primers Euro-71 and Euro-64, corresponding to final map positions 12232 to 12251 and 1231 to 1250 of the noncoding and coding strands, respectively, and a TimeSaver cDNA Synthesis Kit (Pharmacia). .. The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene).

Mutagenesis:

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: Briefly, mutant sense and antisense primers (5′-CACACATTTTCATTCTGCTCATTATGCTGGATTGTC-3′, and 5′-GACAATCCAGCATAATGAGCAGAATGAAAATGTGTG-3′, respectively) were used in PCR amplification, with pAC5.1-hSNM1 as template and Pfu-Turbo DNA polymerase (Stratagene, La Jolla, CA, USA). .. The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA).

Isolation:

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: A full-length hSNM1 cDNA clone was isolated from a lymphoblast cDNA library (a gift from Dr Manuel Buchwald), and sub-cloned into the expression vector pAc5.1a (Invitrogen, Carlsbad, CA, USA) by PCR amplification of the coding region with primers tagged with a Kozak sequence and compatible restriction sites (MfeI and SmaI) (pAc5.1a-EcoRI and BstB1 + Klenow). .. The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA).

Cell Culture:

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: Paragraph title: Cell culture and plasmid construction ... The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA).

Purification:

Article Title: Detection of Androgenic-Mutagenic Compounds and Potential Autochthonous Bacterial Communities during In Situ Bioremediation of Post-methanated Distillery Sludge
Article Snippet: The blunt-ended PCR-amplified 10S rDNA gene products were again purified with Quiaex and ligated into a SmaI-digested dephosphorylated pUC18 vector (Promega, Madison, WI, United States). .. Competent Escherichia coli XL1 blue MRF′ cells (Stratagene) were transformed using the method described by .

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: The precise terminal sequences were confirmed with the Rapid Amplification of cDNA Ends (RACE) technique performed on purified CHV1-Euro7 dsRNA as described by Chen et al. ( ) for CHV1-EP713 dsRNA. .. The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene).

cDNA Library Assay:

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: A full-length hSNM1 cDNA clone was isolated from a lymphoblast cDNA library (a gift from Dr Manuel Buchwald), and sub-cloned into the expression vector pAc5.1a (Invitrogen, Carlsbad, CA, USA) by PCR amplification of the coding region with primers tagged with a Kozak sequence and compatible restriction sites (MfeI and SmaI) (pAc5.1a-EcoRI and BstB1 + Klenow). .. The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA).

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: Paragraph title: Generation of a CHV1-Euro7 cDNA library. ... The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene).

Incubation:

Article Title: Detection of Androgenic-Mutagenic Compounds and Potential Autochthonous Bacterial Communities during In Situ Bioremediation of Post-methanated Distillery Sludge
Article Snippet: The reaction mixture, also contained 10 μl 10 × Klenow buffer [0.5 M Tris HCl (pH 7.5 at 25°C), with 10 mM MgCl2 , 10 mM dithiothreitol, 0.5 mg bovine serum albumin per ml, 1 mM ATP, 200 μM DdATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP also present]; the total volume was 100 μl and the prepared solution was incubated at 37°C for 1.0 h ( ). .. Competent Escherichia coli XL1 blue MRF′ cells (Stratagene) were transformed using the method described by .

Polymerase Chain Reaction:

Article Title: Detection of Androgenic-Mutagenic Compounds and Potential Autochthonous Bacterial Communities during In Situ Bioremediation of Post-methanated Distillery Sludge
Article Snippet: Paragraph title: Cloning of 16S rDNA PCR Products ... Competent Escherichia coli XL1 blue MRF′ cells (Stratagene) were transformed using the method described by .

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: .. The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA). .. Colony PCR followed by BclI digestion (the D736A mutation eliminates a BclI restriction site) was performed to identify the mutant clones.

Article Title: Wolbachia Prophage DNA Adenine Methyltransferase Genes in Different Drosophila-Wolbachia Associations
Article Snippet: .. The PCR products were A-tailed, cloned into the pGEM-T Easy vector (Promega, Wisconsin, USA) and transformed into competent E. coli XL1-Blue MRF cells (Stratagene, Amsterdam, The Netherlands). .. Plasmid DNA was extracted using the Nucleospin Plasmid kit (Macherey-Nagel).

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: Based on the prediction that CHV1-Euro7 RNA may have nucleotide sequence similarity with CHV1-EP713 RNA, first-strand CHV1-Euro7 cDNA synthesized with oligo(dT) as a primer was used as a template for PCR amplification of the terminal domains with primer pairs specific for the 5′ terminus (primers RSDS10 and BR18 [CHV1-EP713 map positions 1 to 22 and 350 to 369, respectively] [ ]) and the 3′ terminus (primers BH23 and RSDS11 [CHV1-EP713 map positions 12131 to 12148 and 12677 to 12697, respectively] [ ]). .. The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene).

Construct:

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: To make the hSNM1-D736A mutant construct, the Quick-Change site-directed mutagenesis method was followed as recommended (Stratagene, La Jolla, CA, USA). .. The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA).

Expressing:

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: A full-length hSNM1 cDNA clone was isolated from a lymphoblast cDNA library (a gift from Dr Manuel Buchwald), and sub-cloned into the expression vector pAc5.1a (Invitrogen, Carlsbad, CA, USA) by PCR amplification of the coding region with primers tagged with a Kozak sequence and compatible restriction sites (MfeI and SmaI) (pAc5.1a-EcoRI and BstB1 + Klenow). .. The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA).

Sequencing:

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: A full-length hSNM1 cDNA clone was isolated from a lymphoblast cDNA library (a gift from Dr Manuel Buchwald), and sub-cloned into the expression vector pAc5.1a (Invitrogen, Carlsbad, CA, USA) by PCR amplification of the coding region with primers tagged with a Kozak sequence and compatible restriction sites (MfeI and SmaI) (pAc5.1a-EcoRI and BstB1 + Klenow). .. The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA).

Article Title: Wolbachia Prophage DNA Adenine Methyltransferase Genes in Different Drosophila-Wolbachia Associations
Article Snippet: To generate DNA templates for sequencing, PCR reactions were done using the Elongase Amplification System (Invitrogen, Glasgow, UK). .. The PCR products were A-tailed, cloned into the pGEM-T Easy vector (Promega, Wisconsin, USA) and transformed into competent E. coli XL1-Blue MRF cells (Stratagene, Amsterdam, The Netherlands).

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: Based on the prediction that CHV1-Euro7 RNA may have nucleotide sequence similarity with CHV1-EP713 RNA, first-strand CHV1-Euro7 cDNA synthesized with oligo(dT) as a primer was used as a template for PCR amplification of the terminal domains with primer pairs specific for the 5′ terminus (primers RSDS10 and BR18 [CHV1-EP713 map positions 1 to 22 and 350 to 369, respectively] [ ]) and the 3′ terminus (primers BH23 and RSDS11 [CHV1-EP713 map positions 12131 to 12148 and 12677 to 12697, respectively] [ ]). .. The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene).

Transformation Assay:

Article Title: Detection of Androgenic-Mutagenic Compounds and Potential Autochthonous Bacterial Communities during In Situ Bioremediation of Post-methanated Distillery Sludge
Article Snippet: .. Competent Escherichia coli XL1 blue MRF′ cells (Stratagene) were transformed using the method described by . ..

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: .. The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA). .. Colony PCR followed by BclI digestion (the D736A mutation eliminates a BclI restriction site) was performed to identify the mutant clones.

Article Title: Wolbachia Prophage DNA Adenine Methyltransferase Genes in Different Drosophila-Wolbachia Associations
Article Snippet: .. The PCR products were A-tailed, cloned into the pGEM-T Easy vector (Promega, Wisconsin, USA) and transformed into competent E. coli XL1-Blue MRF cells (Stratagene, Amsterdam, The Netherlands). .. Plasmid DNA was extracted using the Nucleospin Plasmid kit (Macherey-Nagel).

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: .. The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene). .. The library was screened for larger cloned cDNA inserts, which were subsequently sequenced and analyzed with Genetics Computer Group alignment programs with reference to the published CHV1-EP713 nucleotide sequence ( ).

Rapid Amplification of cDNA Ends:

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: The precise terminal sequences were confirmed with the Rapid Amplification of cDNA Ends (RACE) technique performed on purified CHV1-Euro7 dsRNA as described by Chen et al. ( ) for CHV1-EP713 dsRNA. .. The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene).

Plasmid Preparation:

Article Title: Detection of Androgenic-Mutagenic Compounds and Potential Autochthonous Bacterial Communities during In Situ Bioremediation of Post-methanated Distillery Sludge
Article Snippet: The blunt-ended PCR-amplified 10S rDNA gene products were again purified with Quiaex and ligated into a SmaI-digested dephosphorylated pUC18 vector (Promega, Madison, WI, United States). .. Competent Escherichia coli XL1 blue MRF′ cells (Stratagene) were transformed using the method described by .

Article Title: The hSNM1 protein is a DNA 5?-exonuclease
Article Snippet: Paragraph title: Cell culture and plasmid construction ... The PCR product was digested with DpnI, and transformed into chemically competent E. coli XL1-Blue MRF′ cells (Stratagene, La Jolla, CA, USA).

Article Title: Wolbachia Prophage DNA Adenine Methyltransferase Genes in Different Drosophila-Wolbachia Associations
Article Snippet: .. The PCR products were A-tailed, cloned into the pGEM-T Easy vector (Promega, Wisconsin, USA) and transformed into competent E. coli XL1-Blue MRF cells (Stratagene, Amsterdam, The Netherlands). .. Plasmid DNA was extracted using the Nucleospin Plasmid kit (Macherey-Nagel).

Article Title: Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica
Article Snippet: .. The resulting double-stranded cDNA was ligated into plasmid pUC19, followed by transformation of competent Escherichia coli XL1-Blue MRF′ cells (Stratagene). .. The library was screened for larger cloned cDNA inserts, which were subsequently sequenced and analyzed with Genetics Computer Group alignment programs with reference to the published CHV1-EP713 nucleotide sequence ( ).

DNA Sequencing:

Article Title: Wolbachia Prophage DNA Adenine Methyltransferase Genes in Different Drosophila-Wolbachia Associations
Article Snippet: The PCR products were A-tailed, cloned into the pGEM-T Easy vector (Promega, Wisconsin, USA) and transformed into competent E. coli XL1-Blue MRF cells (Stratagene, Amsterdam, The Netherlands). .. Sequencing reactions were performed with the SequiThermTM Excel Long ReadTM DNA sequencing Kit-LC (Epicentre Technologies) and the DNA sequence of the inserts was determined at the laboratory of Microchemistry, FoRTH, Heraklion (Greece) on a LiCor 4200 DNA sequencer.

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    Stratagene competent e coli xl1 blue mrf cells
    Competent E Coli Xl1 Blue Mrf Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli xl1 blue mrf cells/product/Stratagene
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    competent e coli xl1 blue mrf cells - by Bioz Stars, 2020-04
    85/100 stars
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