competent e coli xl1 blue cells  (Thermo Fisher)


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    Thermo Fisher competent e coli xl1 blue cells
    Competent E Coli Xl1 Blue Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli xl1 blue cells/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    competent e coli xl1 blue cells - by Bioz Stars, 2020-04
    86/100 stars

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    DNA Extraction:

    Article Title: Identification of the bacterial community responsible for traditional fermentation during sour cassava starch, cacha?a and minas cheese production using culture-independent 16s rRNA gene sequence analysis
    Article Snippet: Paragraph title: DNA extraction, amplification and clone libraries ... The amplified fragments were purified using the Wizard system, ligated into the pGEM® -T Vector System II (Promega, Madison, USA) and transformed into competent E. coli (XL1-Blue) cells according to the manufacturer’s instructions (Invitrogen).

    Clone Assay:

    Article Title: Identification of the bacterial community responsible for traditional fermentation during sour cassava starch, cacha?a and minas cheese production using culture-independent 16s rRNA gene sequence analysis
    Article Snippet: The amplified fragments were purified using the Wizard system, ligated into the pGEM® -T Vector System II (Promega, Madison, USA) and transformed into competent E. coli (XL1-Blue) cells according to the manufacturer’s instructions (Invitrogen). .. The clones were stored in freezing medium at -80°C.

    Amplification:

    Article Title: Identification of the bacterial community responsible for traditional fermentation during sour cassava starch, cacha?a and minas cheese production using culture-independent 16s rRNA gene sequence analysis
    Article Snippet: .. The amplified fragments were purified using the Wizard system, ligated into the pGEM® -T Vector System II (Promega, Madison, USA) and transformed into competent E. coli (XL1-Blue) cells according to the manufacturer’s instructions (Invitrogen). .. Blue/white screening on Luria-Bertani (LB) agar containing ampicillin (Sigma), X-gal (Invitrogen) and isopropyl β-D-1-thiogalactopyranoside (IPTG, Promega) was used to randomly select approximately 30 white colonies for each sample.

    Purification:

    Article Title: Identification of the bacterial community responsible for traditional fermentation during sour cassava starch, cacha?a and minas cheese production using culture-independent 16s rRNA gene sequence analysis
    Article Snippet: .. The amplified fragments were purified using the Wizard system, ligated into the pGEM® -T Vector System II (Promega, Madison, USA) and transformed into competent E. coli (XL1-Blue) cells according to the manufacturer’s instructions (Invitrogen). .. Blue/white screening on Luria-Bertani (LB) agar containing ampicillin (Sigma), X-gal (Invitrogen) and isopropyl β-D-1-thiogalactopyranoside (IPTG, Promega) was used to randomly select approximately 30 white colonies for each sample.

    Polymerase Chain Reaction:

    Article Title: Identification of the bacterial community responsible for traditional fermentation during sour cassava starch, cacha?a and minas cheese production using culture-independent 16s rRNA gene sequence analysis
    Article Snippet: PCR reactions were performed in a final reaction volume of 50 µL, containing 0.1–0.5 µg DNA, dNTPs (200µM each), primers (0.8µM each), MgCl2 (1.5 mM), 1 U Taq DNA polymerase (Invitrogen) and the supplied buffer. .. The amplified fragments were purified using the Wizard system, ligated into the pGEM® -T Vector System II (Promega, Madison, USA) and transformed into competent E. coli (XL1-Blue) cells according to the manufacturer’s instructions (Invitrogen).

    Transformation Assay:

    Article Title: Identification of the bacterial community responsible for traditional fermentation during sour cassava starch, cacha?a and minas cheese production using culture-independent 16s rRNA gene sequence analysis
    Article Snippet: .. The amplified fragments were purified using the Wizard system, ligated into the pGEM® -T Vector System II (Promega, Madison, USA) and transformed into competent E. coli (XL1-Blue) cells according to the manufacturer’s instructions (Invitrogen). .. Blue/white screening on Luria-Bertani (LB) agar containing ampicillin (Sigma), X-gal (Invitrogen) and isopropyl β-D-1-thiogalactopyranoside (IPTG, Promega) was used to randomly select approximately 30 white colonies for each sample.

    Plasmid Preparation:

    Article Title: Identification of the bacterial community responsible for traditional fermentation during sour cassava starch, cacha?a and minas cheese production using culture-independent 16s rRNA gene sequence analysis
    Article Snippet: .. The amplified fragments were purified using the Wizard system, ligated into the pGEM® -T Vector System II (Promega, Madison, USA) and transformed into competent E. coli (XL1-Blue) cells according to the manufacturer’s instructions (Invitrogen). .. Blue/white screening on Luria-Bertani (LB) agar containing ampicillin (Sigma), X-gal (Invitrogen) and isopropyl β-D-1-thiogalactopyranoside (IPTG, Promega) was used to randomly select approximately 30 white colonies for each sample.

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  • 93
    Thermo Fisher e coli xl1 blue competent cells
    E Coli Xl1 Blue Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli xl1 blue competent cells/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e coli xl1 blue competent cells - by Bioz Stars, 2020-04
    93/100 stars
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