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Agilent technologies competent e coli xl1 blue cells
Competent E Coli Xl1 Blue Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/competent e coli xl1 blue cells/product/Agilent technologies
Average 94 stars, based on 4 article reviews
Price from $9.99 to $1999.99
competent e coli xl1 blue cells - by Bioz Stars, 2020-04
94/100 stars

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Clone Assay:

Article Title: Development and implementation of a cell-based assay to discover agonists of the nuclear receptor REV-ERBα
Article Snippet: The pGal4-hPXR expression vector contains DEF regions of human PXR cloned into the Gal4 plasmid [ ]. .. After heat shock of competent E. coli XL1 blue cells (Agilent Technologies, CA, USA), isolation and purification of plasmids were performed using EndoFree (endotoxin free) plasmid purification kit (QIAGEN, Hilden, Germany) as instructed by the manufacturer.

Article Title: Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis
Article Snippet: DNA-modifying enzymes (high-fidelity DNA polymerase Q5, Taq DNA polymerase, T4 DNA ligase, restriction endonucleases) and the NEBuilder HiFi DNA Assembly Master Mix were obtained from New England BioLabs (NEB), Inc. (Ipswich, MA, USA), and the In-fusion HD Cloning kit from Takara Bio, Inc. (Mountain View, CA, USA). .. Competent E. coli XL1-blue cells (Agilent Technologies, Santa Clara, CA, USA) were transformed following the protocol provided by the manufacturer.

Article Title: Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae) 1
Article Snippet: .. Twenty microliters of the enriched DNA was PCR amplified with the same conditions used in the previous reaction, cloned using pGEM-T Easy Vector (Promega Corporation) according to the protocol suggested by the manufacturer, and transformed in chemically competent Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, California, USA). .. A total of 192 positive clones were sequenced with a MegaBACE 1000 automated sequencer (GE Healthcare Biosciences, Pittsburgh, Pennsylvania, USA).

Screening Assay:

Article Title: Development and implementation of a cell-based assay to discover agonists of the nuclear receptor REV-ERBα
Article Snippet: For the secondary screening assay, the pGAL4-hCAR1 expression vector was created by cloning full length human CAR1 (NM_005122.4) sequence in the Gal4 plasmid. .. After heat shock of competent E. coli XL1 blue cells (Agilent Technologies, CA, USA), isolation and purification of plasmids were performed using EndoFree (endotoxin free) plasmid purification kit (QIAGEN, Hilden, Germany) as instructed by the manufacturer.

Amplification:

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: This fragment was obtained by PCR amplification (DreamTaq polymerase) of the 2 μm of pROS10 using primers containing the specific 20 bp sgRNA recognition (target) sequence and a 50 bp sequence, homologous to the linearized plasmid backbone. .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: This fragment was obtained by PCR amplification (DreamTaq polymerase) of the 2 μm of pROS10 using primers containing the specific 20 bp sgRNA recognition (target) sequence and a 50 bp sequence, homologous to the linearized plasmid backbone. .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Article Title: Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae) 1
Article Snippet: .. Twenty microliters of the enriched DNA was PCR amplified with the same conditions used in the previous reaction, cloned using pGEM-T Easy Vector (Promega Corporation) according to the protocol suggested by the manufacturer, and transformed in chemically competent Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, California, USA). .. A total of 192 positive clones were sequenced with a MegaBACE 1000 automated sequencer (GE Healthcare Biosciences, Pittsburgh, Pennsylvania, USA).

In Vitro:

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: The sgRNA plasmids were assembled in vitro from two DNA parts (using NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs). .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: The sgRNA plasmids were assembled in vitro from two DNA parts (using NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs). .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Ligation:

Article Title: Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae) 1
Article Snippet: The reaction was performed in a 50-μL reaction containing 5 μL of the ligation reaction, 10 μM of Afa21 adapter, 0.2 mM of each dNTP (Fermentas, Vilnius, Lithuania), 1× Taq buffer (Fermentas), 1.5 mM of magnesium chloride (Fermentas), and 1 unit of Taq DNA Polymerase (Fermentas). .. Twenty microliters of the enriched DNA was PCR amplified with the same conditions used in the previous reaction, cloned using pGEM-T Easy Vector (Promega Corporation) according to the protocol suggested by the manufacturer, and transformed in chemically competent Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, California, USA).

Isolation:

Article Title: Development and implementation of a cell-based assay to discover agonists of the nuclear receptor REV-ERBα
Article Snippet: .. After heat shock of competent E. coli XL1 blue cells (Agilent Technologies, CA, USA), isolation and purification of plasmids were performed using EndoFree (endotoxin free) plasmid purification kit (QIAGEN, Hilden, Germany) as instructed by the manufacturer. ..

Polymerase Chain Reaction:

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: This fragment was obtained by PCR amplification (DreamTaq polymerase) of the 2 μm of pROS10 using primers containing the specific 20 bp sgRNA recognition (target) sequence and a 50 bp sequence, homologous to the linearized plasmid backbone. .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: This fragment was obtained by PCR amplification (DreamTaq polymerase) of the 2 μm of pROS10 using primers containing the specific 20 bp sgRNA recognition (target) sequence and a 50 bp sequence, homologous to the linearized plasmid backbone. .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Article Title: Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae) 1
Article Snippet: .. Twenty microliters of the enriched DNA was PCR amplified with the same conditions used in the previous reaction, cloned using pGEM-T Easy Vector (Promega Corporation) according to the protocol suggested by the manufacturer, and transformed in chemically competent Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, California, USA). .. A total of 192 positive clones were sequenced with a MegaBACE 1000 automated sequencer (GE Healthcare Biosciences, Pittsburgh, Pennsylvania, USA).

Cell Culture:

Article Title: Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis
Article Snippet: Competent E. coli XL1-blue cells (Agilent Technologies, Santa Clara, CA, USA) were transformed following the protocol provided by the manufacturer. .. Schizosaccharomyces pombe strains (Table ) were cultured on yeast extract (YE), and on yeast nitrogen base glutamate (YNG) agar plates containing the required supplements (concentration 250 μg/ml on YE, and 75 μg/ml on YNG).

Purification:

Article Title: Development and implementation of a cell-based assay to discover agonists of the nuclear receptor REV-ERBα
Article Snippet: .. After heat shock of competent E. coli XL1 blue cells (Agilent Technologies, CA, USA), isolation and purification of plasmids were performed using EndoFree (endotoxin free) plasmid purification kit (QIAGEN, Hilden, Germany) as instructed by the manufacturer. ..

Concentration Assay:

Article Title: Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis
Article Snippet: Competent E. coli XL1-blue cells (Agilent Technologies, Santa Clara, CA, USA) were transformed following the protocol provided by the manufacturer. .. Schizosaccharomyces pombe strains (Table ) were cultured on yeast extract (YE), and on yeast nitrogen base glutamate (YNG) agar plates containing the required supplements (concentration 250 μg/ml on YE, and 75 μg/ml on YNG).

Incubation:

Article Title: Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae) 1
Article Snippet: The reaction was incubated at 20°C for 2 h, followed by enzyme inactivation at 70°C for 10 min. Fragments were PCR amplified using a Veriti Thermal Cycler (Life Technologies, Carlsbad, California, USA) to yield adequate DNA for the next step. .. Twenty microliters of the enriched DNA was PCR amplified with the same conditions used in the previous reaction, cloned using pGEM-T Easy Vector (Promega Corporation) according to the protocol suggested by the manufacturer, and transformed in chemically competent Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, California, USA).

Luciferase:

Article Title: Development and implementation of a cell-based assay to discover agonists of the nuclear receptor REV-ERBα
Article Snippet: The reporter vector contains luciferase gene under control of Gal4 response element (UAS) and minimal TK promoter sequences [ ] and the normalization vector pCMV-Ren was from Promega Inc., USA. .. After heat shock of competent E. coli XL1 blue cells (Agilent Technologies, CA, USA), isolation and purification of plasmids were performed using EndoFree (endotoxin free) plasmid purification kit (QIAGEN, Hilden, Germany) as instructed by the manufacturer.

DNA Sequencing:

Article Title: Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis
Article Snippet: Competent E. coli XL1-blue cells (Agilent Technologies, Santa Clara, CA, USA) were transformed following the protocol provided by the manufacturer. .. Competent E. coli XL1-blue cells (Agilent Technologies, Santa Clara, CA, USA) were transformed following the protocol provided by the manufacturer.

Expressing:

Article Title: Development and implementation of a cell-based assay to discover agonists of the nuclear receptor REV-ERBα
Article Snippet: The pGal4-hPXR expression vector contains DEF regions of human PXR cloned into the Gal4 plasmid [ ]. .. After heat shock of competent E. coli XL1 blue cells (Agilent Technologies, CA, USA), isolation and purification of plasmids were performed using EndoFree (endotoxin free) plasmid purification kit (QIAGEN, Hilden, Germany) as instructed by the manufacturer.

Sequencing:

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: This fragment was obtained by PCR amplification (DreamTaq polymerase) of the 2 μm of pROS10 using primers containing the specific 20 bp sgRNA recognition (target) sequence and a 50 bp sequence, homologous to the linearized plasmid backbone. .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: This fragment was obtained by PCR amplification (DreamTaq polymerase) of the 2 μm of pROS10 using primers containing the specific 20 bp sgRNA recognition (target) sequence and a 50 bp sequence, homologous to the linearized plasmid backbone. .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Article Title: Development and implementation of a cell-based assay to discover agonists of the nuclear receptor REV-ERBα
Article Snippet: For the secondary screening assay, the pGAL4-hCAR1 expression vector was created by cloning full length human CAR1 (NM_005122.4) sequence in the Gal4 plasmid. .. After heat shock of competent E. coli XL1 blue cells (Agilent Technologies, CA, USA), isolation and purification of plasmids were performed using EndoFree (endotoxin free) plasmid purification kit (QIAGEN, Hilden, Germany) as instructed by the manufacturer.

Article Title: Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae) 1
Article Snippet: A Veriti Thermal Cycler (Life Technologies) was used to amplify the ligated DNA with the following sequence: 95°C for 4 min; followed by 20 cycles of 94°C for 30 s, 60°C for 1 min, 72°C for 1 min; and a final extension step at 72°C for 8 min. .. Twenty microliters of the enriched DNA was PCR amplified with the same conditions used in the previous reaction, cloned using pGEM-T Easy Vector (Promega Corporation) according to the protocol suggested by the manufacturer, and transformed in chemically competent Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, California, USA).

Transformation Assay:

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin. .. The E. coli colonies were picked and mixed directly in the DreamTaq PCR mix, containing primers binding to the specific 20 bp recognition sequence (primers 11662-11670, 11756) together with two primers binding in the plasmid backbone (primers 4034 and 5941), confirming the presence of one or two target sgRNAs.

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin. .. The E. coli colonies were picked and mixed directly in the DreamTaq PCR mix, containing primers binding to the specific 20 bp recognition sequence (primers 11662-11670, 11756) together with two primers binding in the plasmid backbone (primers 4034 and 5941), confirming the presence of one or two target sgRNAs.

Article Title: Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis
Article Snippet: .. Competent E. coli XL1-blue cells (Agilent Technologies, Santa Clara, CA, USA) were transformed following the protocol provided by the manufacturer. .. Schizosaccharomyces pombe strains (Table ) were cultured on yeast extract (YE), and on yeast nitrogen base glutamate (YNG) agar plates containing the required supplements (concentration 250 μg/ml on YE, and 75 μg/ml on YNG).

Article Title: Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae) 1
Article Snippet: .. Twenty microliters of the enriched DNA was PCR amplified with the same conditions used in the previous reaction, cloned using pGEM-T Easy Vector (Promega Corporation) according to the protocol suggested by the manufacturer, and transformed in chemically competent Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, California, USA). .. A total of 192 positive clones were sequenced with a MegaBACE 1000 automated sequencer (GE Healthcare Biosciences, Pittsburgh, Pennsylvania, USA).

Binding Assay:

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: The first part was the plasmid backbone obtained by PCR with Phusion polymerase, using a single primer (6005, Supplemental Material ) binding at each of the two SNR52 promoters of either pROS10, pROS14 or pROS16 (Table ; Mans et al. ). .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: The first part was the plasmid backbone obtained by PCR with Phusion polymerase, using a single primer (6005, Supplemental Material ) binding at each of the two SNR52 promoters of either pROS10, pROS14 or pROS16 (Table ; Mans et al. ). .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin.

Plasmid Preparation:

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin. .. The E. coli colonies were picked and mixed directly in the DreamTaq PCR mix, containing primers binding to the specific 20 bp recognition sequence (primers 11662-11670, 11756) together with two primers binding in the plasmid backbone (primers 4034 and 5941), confirming the presence of one or two target sgRNAs.

Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
Article Snippet: .. Plasmid assembly was followed by chemical transformation to chemically competent E. coli XL1-blue cells according to the supplier's instructions (Agilent Technologies, Santa Clara, CA) for storage and plasmid propagation, in Lysogeny Broth (LB) medium with 100 mg L−1 ampicillin. .. The E. coli colonies were picked and mixed directly in the DreamTaq PCR mix, containing primers binding to the specific 20 bp recognition sequence (primers 11662-11670, 11756) together with two primers binding in the plasmid backbone (primers 4034 and 5941), confirming the presence of one or two target sgRNAs.

Article Title: Development and implementation of a cell-based assay to discover agonists of the nuclear receptor REV-ERBα
Article Snippet: .. After heat shock of competent E. coli XL1 blue cells (Agilent Technologies, CA, USA), isolation and purification of plasmids were performed using EndoFree (endotoxin free) plasmid purification kit (QIAGEN, Hilden, Germany) as instructed by the manufacturer. ..

Article Title: Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis
Article Snippet: Plasmid sequences are available as supporting online material (Lorenz ). .. Competent E. coli XL1-blue cells (Agilent Technologies, Santa Clara, CA, USA) were transformed following the protocol provided by the manufacturer.

Article Title: Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae) 1
Article Snippet: .. Twenty microliters of the enriched DNA was PCR amplified with the same conditions used in the previous reaction, cloned using pGEM-T Easy Vector (Promega Corporation) according to the protocol suggested by the manufacturer, and transformed in chemically competent Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, California, USA). .. A total of 192 positive clones were sequenced with a MegaBACE 1000 automated sequencer (GE Healthcare Biosciences, Pittsburgh, Pennsylvania, USA).

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    Agilent technologies competent e coli xl1 blue cells
    Competent E Coli Xl1 Blue Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli xl1 blue cells/product/Agilent technologies
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    competent e coli xl1 blue cells - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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