competent e coli novablue singles cells  (Thermo Fisher)


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    Name:
    Competent Cell Sampler
    Description:
    Our Competent Cell Sampler includes two reactions of four chemically competent E coli strains One Shot Mach1 T1R catalog C862003 is the fastest growing chemically competent strain currently available Mach1 T1R colonies are clearly visible within eight hours of plating your transformation From an overnight colony minipreps can be performed after four hours of growth Genotype F Φ80lacZΔM15 ΔlacX74 hsdR rK mK ΔrecA1398 endA1 tonA One Shot OmniMAX 2 T1R cells catalog C854003 are perfect for use in all cloning applications including Gateway technology They offer the highest transformation efficiency of any chemically competent cell in the One Shot packaging format Genotype F proAB lacIq lacZΔM15 Tn10 TetR Δ ccdAB mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 Δ lacZYA argF U169 endA1 recA1 supE44 thi 1 gyrA96 relA1 tonA panD One Shot Stbl3 catalog C7373 03 reduce the frequency of unwanted homologous recombination and so are perfect for cloning unstable DNA Genotype F mcrB mrr hsdS20 rB mB recA13 supE44 ara 14 galK2 lacY1 proA2 rpsL20 StrR xyl 5 leu mtl 1 One Shot TOP10 cells C4040 03 are perfect for routine cloning and included in many TOPO cloning and expression kits Genotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ ara leu 7697 galU galK rpsL StrR endA1 nupGTry them all to optimize transformations for your favorite clones
    Catalog Number:
    a10469
    Price:
    None
    Applications:
    Chemically Competent Cells for Cloning|Cloning|Transformation
    Category:
    Competent Cells Strains
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    Structured Review

    Thermo Fisher competent e coli novablue singles cells
    Our Competent Cell Sampler includes two reactions of four chemically competent E coli strains One Shot Mach1 T1R catalog C862003 is the fastest growing chemically competent strain currently available Mach1 T1R colonies are clearly visible within eight hours of plating your transformation From an overnight colony minipreps can be performed after four hours of growth Genotype F Φ80lacZΔM15 ΔlacX74 hsdR rK mK ΔrecA1398 endA1 tonA One Shot OmniMAX 2 T1R cells catalog C854003 are perfect for use in all cloning applications including Gateway technology They offer the highest transformation efficiency of any chemically competent cell in the One Shot packaging format Genotype F proAB lacIq lacZΔM15 Tn10 TetR Δ ccdAB mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 Δ lacZYA argF U169 endA1 recA1 supE44 thi 1 gyrA96 relA1 tonA panD One Shot Stbl3 catalog C7373 03 reduce the frequency of unwanted homologous recombination and so are perfect for cloning unstable DNA Genotype F mcrB mrr hsdS20 rB mB recA13 supE44 ara 14 galK2 lacY1 proA2 rpsL20 StrR xyl 5 leu mtl 1 One Shot TOP10 cells C4040 03 are perfect for routine cloning and included in many TOPO cloning and expression kits Genotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ ara leu 7697 galU galK rpsL StrR endA1 nupGTry them all to optimize transformations for your favorite clones
    https://www.bioz.com/result/competent e coli novablue singles cells/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    competent e coli novablue singles cells - by Bioz Stars, 2020-08
    99/100 stars

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    recombinant vector

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    Related Articles

    Clone Assay:

    Article Title: Crystallographic Insights into the Pore Structures and Mechanisms of the EutL and EutM Shell Proteins of the Ethanolamine-Utilizing Microcompartment of Escherichia coli ▿ ▿ †
    Article Snippet: .. The resulting products were cloned into a pET101 vector (Invitrogen) according to the company's protocol and were subsequently transformed into chemically competent TOP10 cells (Invitrogen). .. Plasmids of clones that were positively identified by PCR were transformed into BL21-AI cells for overexpression.

    Article Title: A Diverse Range of Bacterial and Eukaryotic Chitinases Hydrolyzes the LacNAc (Galβ1–4GlcNAc) and LacdiNAc (GalNAcβ1–4GlcNAc) Motifs Found on Vertebrate and Insect Cells *
    Article Snippet: .. The synthetic genes were cloned into pET15b expression vectors, which were transformed into chemically competent E. coli TOP10 cells (Invitrogen) for long term storage. .. For protein expression the vector carrying SG1474 was transformed into E. coli BL21(DE3) cells (Novagen, Darmstadt, Germany), whereas the vector carrying was transformed into E. coli BL21/TUNERTM (DE3) cells (Novagen).

    Ligation:

    Article Title: Generation of Human PTH1R Construct with Flag Epitope Located Internally: Comparison of Two-Fragment Assembly by Using PCR Overlap Extension or Ligase
    Article Snippet: .. The ligation mixture was then transformed to competent cells (Invitrogen) and screened by ampicillin. ..

    Article Title: An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening *
    Article Snippet: .. After an inactivation step at 70 °C for 5 min to inactivate the T4 polynucleotide kinase, the PCR products were circularized using 350 U T4 DNA ligase (Takara) at 12 °C for 16 h. 10 μl of the ligation mixture was then used to transform competent E. coli Top10 (Invitrogen) and the transformation mixture was plated on a Luria-Bertani (LB) agar plate containing 100 μg/ml ampicillin and incubated at 37 °C. .. Ten colonies were picked up randomly and the plasmid DNA was extracted and digested by the designed restriction enzyme Xho I along with another enzyme Bgl II.

    Construct:

    Article Title: The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol
    Article Snippet: .. This construct was transformed into TOP10 competent cells (Invitrogen) for expression. ..

    Incubation:

    Article Title: What Goes in Must Come out: Testing for Biases in Molecular Analysis of Arbuscular Mycorrhizal Fungal Communities
    Article Snippet: .. Vectors (4µl) were transformed into Escherichia coli cells by incubation with 25µl competent E. coli cells (DH5α; Invitrogen, Renfrewshire, UK) at 4°C for 30 minutes, followed by a heatshock of 42°C for 45 seconds and rotary incubation with 475µl Super Optimal broth with Catabolite repression (SOC) at 37°C for 1 hour. ..

    Article Title: An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening *
    Article Snippet: .. After an inactivation step at 70 °C for 5 min to inactivate the T4 polynucleotide kinase, the PCR products were circularized using 350 U T4 DNA ligase (Takara) at 12 °C for 16 h. 10 μl of the ligation mixture was then used to transform competent E. coli Top10 (Invitrogen) and the transformation mixture was plated on a Luria-Bertani (LB) agar plate containing 100 μg/ml ampicillin and incubated at 37 °C. .. Ten colonies were picked up randomly and the plasmid DNA was extracted and digested by the designed restriction enzyme Xho I along with another enzyme Bgl II.

    other:

    Article Title: Ex vivo tools for the clonal analysis of zebrafish hematopoiesis
    Article Snippet: BL21-CodonPlus (DE3)-RIL competent cells (Agilent Technologies, cat. no. 230245-41) LB broth, Miller (Sigma-Aldrich, cat. no. L2542) sf21 insect cells (Gibco, cat. no. 11497-013) Sf-900 II SFM insect medium (Gibco, cat. no. 10902-096) Top10 competent cells (Invitrogen, cat. no. C4040-10)

    Expressing:

    Article Title: The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol
    Article Snippet: .. This construct was transformed into TOP10 competent cells (Invitrogen) for expression. ..

    Article Title: A Diverse Range of Bacterial and Eukaryotic Chitinases Hydrolyzes the LacNAc (Galβ1–4GlcNAc) and LacdiNAc (GalNAcβ1–4GlcNAc) Motifs Found on Vertebrate and Insect Cells *
    Article Snippet: .. The synthetic genes were cloned into pET15b expression vectors, which were transformed into chemically competent E. coli TOP10 cells (Invitrogen) for long term storage. .. For protein expression the vector carrying SG1474 was transformed into E. coli BL21(DE3) cells (Novagen, Darmstadt, Germany), whereas the vector carrying was transformed into E. coli BL21/TUNERTM (DE3) cells (Novagen).

    Polymerase Chain Reaction:

    Article Title: An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening *
    Article Snippet: .. After an inactivation step at 70 °C for 5 min to inactivate the T4 polynucleotide kinase, the PCR products were circularized using 350 U T4 DNA ligase (Takara) at 12 °C for 16 h. 10 μl of the ligation mixture was then used to transform competent E. coli Top10 (Invitrogen) and the transformation mixture was plated on a Luria-Bertani (LB) agar plate containing 100 μg/ml ampicillin and incubated at 37 °C. .. Ten colonies were picked up randomly and the plasmid DNA was extracted and digested by the designed restriction enzyme Xho I along with another enzyme Bgl II.

    Transformation Assay:

    Article Title: Generation of Human PTH1R Construct with Flag Epitope Located Internally: Comparison of Two-Fragment Assembly by Using PCR Overlap Extension or Ligase
    Article Snippet: .. The ligation mixture was then transformed to competent cells (Invitrogen) and screened by ampicillin. ..

    Article Title: Crystallographic Insights into the Pore Structures and Mechanisms of the EutL and EutM Shell Proteins of the Ethanolamine-Utilizing Microcompartment of Escherichia coli ▿ ▿ †
    Article Snippet: .. The resulting products were cloned into a pET101 vector (Invitrogen) according to the company's protocol and were subsequently transformed into chemically competent TOP10 cells (Invitrogen). .. Plasmids of clones that were positively identified by PCR were transformed into BL21-AI cells for overexpression.

    Article Title: What Goes in Must Come out: Testing for Biases in Molecular Analysis of Arbuscular Mycorrhizal Fungal Communities
    Article Snippet: .. Vectors (4µl) were transformed into Escherichia coli cells by incubation with 25µl competent E. coli cells (DH5α; Invitrogen, Renfrewshire, UK) at 4°C for 30 minutes, followed by a heatshock of 42°C for 45 seconds and rotary incubation with 475µl Super Optimal broth with Catabolite repression (SOC) at 37°C for 1 hour. ..

    Article Title: The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol
    Article Snippet: .. This construct was transformed into TOP10 competent cells (Invitrogen) for expression. ..

    Article Title: An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening *
    Article Snippet: .. After an inactivation step at 70 °C for 5 min to inactivate the T4 polynucleotide kinase, the PCR products were circularized using 350 U T4 DNA ligase (Takara) at 12 °C for 16 h. 10 μl of the ligation mixture was then used to transform competent E. coli Top10 (Invitrogen) and the transformation mixture was plated on a Luria-Bertani (LB) agar plate containing 100 μg/ml ampicillin and incubated at 37 °C. .. Ten colonies were picked up randomly and the plasmid DNA was extracted and digested by the designed restriction enzyme Xho I along with another enzyme Bgl II.

    Article Title: A Diverse Range of Bacterial and Eukaryotic Chitinases Hydrolyzes the LacNAc (Galβ1–4GlcNAc) and LacdiNAc (GalNAcβ1–4GlcNAc) Motifs Found on Vertebrate and Insect Cells *
    Article Snippet: .. The synthetic genes were cloned into pET15b expression vectors, which were transformed into chemically competent E. coli TOP10 cells (Invitrogen) for long term storage. .. For protein expression the vector carrying SG1474 was transformed into E. coli BL21(DE3) cells (Novagen, Darmstadt, Germany), whereas the vector carrying was transformed into E. coli BL21/TUNERTM (DE3) cells (Novagen).

    Plasmid Preparation:

    Article Title: Crystallographic Insights into the Pore Structures and Mechanisms of the EutL and EutM Shell Proteins of the Ethanolamine-Utilizing Microcompartment of Escherichia coli ▿ ▿ †
    Article Snippet: .. The resulting products were cloned into a pET101 vector (Invitrogen) according to the company's protocol and were subsequently transformed into chemically competent TOP10 cells (Invitrogen). .. Plasmids of clones that were positively identified by PCR were transformed into BL21-AI cells for overexpression.

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  • 84
    Thermo Fisher competent cell sampler
    Competent Cell Sampler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent cell sampler/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    competent cell sampler - by Bioz Stars, 2020-08
    84/100 stars
      Buy from Supplier

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