competent e coli novablue cells  (Millipore)


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    Structured Review

    Millipore competent e coli novablue cells
    Competent E Coli Novablue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli novablue cells/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    competent e coli novablue cells - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Electroporation:

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli
    Article Snippet: .. Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes. .. These recombinant strains were subsequently transformed using the helper plasmids pBB540/pBB542 or pTUM4.1.

    Positron Emission Tomography:

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli
    Article Snippet: .. Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes. .. These recombinant strains were subsequently transformed using the helper plasmids pBB540/pBB542 or pTUM4.1.

    Article Title: YeeV is an Escherichia coli Toxin that Inhibits Cell Division by Targeting the Cytoskeleton Proteins, FtsZ and MreB
    Article Snippet: .. E. coli BW25113 ( ΔaraBD ) (Datsenko and Wanner, 2000), BL21 (DE3) were grown in M9 medium at 37° C. Plasmids pET-28a- yeeV , pET-28a- ftsZ , pET-28a- mreB and pET-28a -sulA were constructed in pET-28a (Novagen) with an N-terminal His6 tag. .. Plasmids pBAD33- yeeV and pBAD33- sulA were constructed from pBAD33 (Guzman et al., 1995) to tightly regulate gene expression by the addition of arabinose.

    Construct:

    Article Title: YeeV is an Escherichia coli Toxin that Inhibits Cell Division by Targeting the Cytoskeleton Proteins, FtsZ and MreB
    Article Snippet: .. E. coli BW25113 ( ΔaraBD ) (Datsenko and Wanner, 2000), BL21 (DE3) were grown in M9 medium at 37° C. Plasmids pET-28a- yeeV , pET-28a- ftsZ , pET-28a- mreB and pET-28a -sulA were constructed in pET-28a (Novagen) with an N-terminal His6 tag. .. Plasmids pBAD33- yeeV and pBAD33- sulA were constructed from pBAD33 (Guzman et al., 1995) to tightly regulate gene expression by the addition of arabinose.

    Article Title: Characterization of gene expression on genomic segment 7 of infectious salmon anaemia virus
    Article Snippet: .. The plasmid constructs were used to transform competent E. coli strain BL21(DE3)(pLysS) (Novagen) in order to express the GST fusion proteins. ..

    Purification:

    Article Title: Localization of a Class III Myosin to Filopodia Tips in Transfected HeLa Cells Requires an Actin-binding Site in its Tail Domain
    Article Snippet: .. Recombinant GST proteins were expressed from pGEX (Pharmacia) vectors in BL21[DE3] E. coli cell line and purified using glutathione-agarose beads (Sigma, St. Louis, MO) per manufacturers protocol. .. Actin filaments were formed in vitro from mammalian actin monomers as described ( ).

    Expressing:

    Article Title: Fatty acid DSF binds and allosterically activates histidine kinase RpfC of phytopathogenic bacterium Xanthomonas campestris pv. campestris to regulate quorum-sensing and virulence
    Article Snippet: .. E . coli BL21(DE3) strain was used as the host for expressing recombinant proteins with pET30a vector (Novagen, USA). ..

    Article Title: Immunization with 3-oxododecanoyl-L-homoserine lactone-r-PcrV conjugate enhances survival of mice against lethal burn infections caused by Pseudomonas aeruginosa
    Article Snippet: .. Escherichia (E.) coli strains DH5a and Bl21 (DE3) (Novagen Co., Wisconsin, USA) were used for clone and expression of recombinant PcrV protein (r-PcrV), respectively. .. Luria-Bertani (LB) broth, agar (Merck Co., Germany) and Pseudomonas Selective Agar were used for the bacterial culturing.

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli
    Article Snippet: .. Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes. .. These recombinant strains were subsequently transformed using the helper plasmids pBB540/pBB542 or pTUM4.1.

    Transformation Assay:

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli
    Article Snippet: .. Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes. .. These recombinant strains were subsequently transformed using the helper plasmids pBB540/pBB542 or pTUM4.1.

    Recombinant:

    Article Title: Expression and Functional Characterization of Membrane-Integrated Mammalian Corticotropin Releasing Factor Receptors 1 and 2 in Escherichia coli
    Article Snippet: .. For recombinant protein production: BL21(DE3) [F− ompT hsdSB (rB − mB − ) gal dcm (DE3)] (Novagen), OverExpress C41(DE3) and C43(DE3), which are derivative of BL21(DE3) containing genetic mutations phenotypically selected for conferring tolerance to toxic proteins (Lucigen), and Rosetta2(DE3) [F− ompT hsdS B (rB − mB − ) gal dcm (DE3) pRARE2 (CamR )] (Novagen) providing tRNAs for seven E. coli rare codons. .. For DNA vectors cloning and propagation: One Shot® TOP10 (Life Technologies).

    Article Title: Fatty acid DSF binds and allosterically activates histidine kinase RpfC of phytopathogenic bacterium Xanthomonas campestris pv. campestris to regulate quorum-sensing and virulence
    Article Snippet: .. E . coli BL21(DE3) strain was used as the host for expressing recombinant proteins with pET30a vector (Novagen, USA). ..

    Article Title: Immunization with 3-oxododecanoyl-L-homoserine lactone-r-PcrV conjugate enhances survival of mice against lethal burn infections caused by Pseudomonas aeruginosa
    Article Snippet: .. Escherichia (E.) coli strains DH5a and Bl21 (DE3) (Novagen Co., Wisconsin, USA) were used for clone and expression of recombinant PcrV protein (r-PcrV), respectively. .. Luria-Bertani (LB) broth, agar (Merck Co., Germany) and Pseudomonas Selective Agar were used for the bacterial culturing.

    Article Title: Localization of a Class III Myosin to Filopodia Tips in Transfected HeLa Cells Requires an Actin-binding Site in its Tail Domain
    Article Snippet: .. Recombinant GST proteins were expressed from pGEX (Pharmacia) vectors in BL21[DE3] E. coli cell line and purified using glutathione-agarose beads (Sigma, St. Louis, MO) per manufacturers protocol. .. Actin filaments were formed in vitro from mammalian actin monomers as described ( ).

    Plasmid Preparation:

    Article Title: Fatty acid DSF binds and allosterically activates histidine kinase RpfC of phytopathogenic bacterium Xanthomonas campestris pv. campestris to regulate quorum-sensing and virulence
    Article Snippet: .. E . coli BL21(DE3) strain was used as the host for expressing recombinant proteins with pET30a vector (Novagen, USA). ..

    Article Title: Characterization of gene expression on genomic segment 7 of infectious salmon anaemia virus
    Article Snippet: .. The plasmid constructs were used to transform competent E. coli strain BL21(DE3)(pLysS) (Novagen) in order to express the GST fusion proteins. ..

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  • 86
    Millipore e coli novablue competent cells
    E Coli Novablue Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue competent cells/product/Millipore
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    e coli novablue competent cells - by Bioz Stars, 2020-05
    86/100 stars
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