Structured Review

Stratagene competent e coli cells
Competent E Coli Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/competent e coli cells/product/Stratagene
Average 92 stars, based on 8 article reviews
Price from $9.99 to $1999.99
competent e coli cells - by Bioz Stars, 2020-08
92/100 stars

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Clone Assay:

Article Title: A Set of Genes Encoding a Second Toluene Efflux System in Pseudomonas putida DOT-T1E Is Linked to the tod Genes for Toluene Metabolism
Article Snippet: .. Competent E. coli cells were prepared according to the method of Inoue et al. ( ). pUC19 ( ) and pBS(SK−) (Stratagene, Inc.) were used for cloning experiments. .. The helper plasmid pRK600 was used to mobilize tra -lacking mob+ plasmids ( ).

Article Title: qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin ▿
Article Snippet: .. Competent Escherichia coli cells were used in the transformation and cloning experiments: TG1 cells (Stratagene, Cambridge, United Kingdom) were used in the initial transformation experiments with the natural plasmid and cloned plasmid fragment, and DH10B cells (Invitrogen, Cergy Pontoise, France) were used for transformation with the native plasmid and vector pBR322, containing the resistance genes qnrD , qnrA1 , and qnrS1 . .. Plasmids pACYC177 (New England Biolabs, Hitchin, United Kingdom), containing resistance to ampicillin and kanamycin, and pBR322 (New England Biolabs, Hitchin, United Kingdom), containing resistance to tetracycline and ampicillin, were used in the cloning of plasmid restriction fragments and PCR products.

Transfection:

Article Title: Cholinergic signaling inhibits oxalate transport by human intestinal T84 cells
Article Snippet: .. Transfection-grade plasmid DNA was prepared by transforming these plasmids into competent Escherichia coli cells following Stratagene's protocol. .. To accomplish SLC26A6 knockdown in T84 cells, 2 × 106 cells were untransfected (UT) or nucleofected with 12 μg of shRNA plasmid DNA (NC = negative control shRNA or S1 and S2 = shRNAs targeting SLC26A6) using the Cell Line Nucleofector Kit T (Amaxa: catalog no. VCA-1002; Program T-005).

Marker:

Article Title: Characterization of the Enzymatic Component of the ADP-Ribosyltransferase Toxin CDTa from Clostridium difficile
Article Snippet: .. Oligonucleotides were purchased from MWG (Ebersberg, Germany), the pGEX2T vector (included in the glutathione S -transferase [GST] Gene Fusion System) and glutathione Sepharose 4B were obtained from Pharmacia Biotech (Uppsala, Sweden), the DNA molecular weight marker (lambda Hin dIII) and restriction enzymes were from Roche Diagnostics (Mannheim, Germany), and T4 ligase and competent E. coli cells were from Stratagene (Heidelberg, Germany). .. The Topo-TA-Cloning kit was from Invitrogen (Groningen, The Netherlands).

Molecular Weight:

Article Title: Characterization of the Enzymatic Component of the ADP-Ribosyltransferase Toxin CDTa from Clostridium difficile
Article Snippet: .. Oligonucleotides were purchased from MWG (Ebersberg, Germany), the pGEX2T vector (included in the glutathione S -transferase [GST] Gene Fusion System) and glutathione Sepharose 4B were obtained from Pharmacia Biotech (Uppsala, Sweden), the DNA molecular weight marker (lambda Hin dIII) and restriction enzymes were from Roche Diagnostics (Mannheim, Germany), and T4 ligase and competent E. coli cells were from Stratagene (Heidelberg, Germany). .. The Topo-TA-Cloning kit was from Invitrogen (Groningen, The Netherlands).

Polymerase Chain Reaction:

Article Title: Conformational changes during human P2X7 receptor activation examined by structural modelling and cysteine-based cross-linking studies
Article Snippet: .. The PCR product, after treatment with DpnI (Thermo Fisher Scientific) for 60 min, was transformed into competent E. coli cells (Stratagene). .. Small-scale isolation of plasmid was performed using a mini-DNA preparation kit (QIAGEN).

Transformation Assay:

Article Title: Conformational changes during human P2X7 receptor activation examined by structural modelling and cysteine-based cross-linking studies
Article Snippet: .. The PCR product, after treatment with DpnI (Thermo Fisher Scientific) for 60 min, was transformed into competent E. coli cells (Stratagene). .. Small-scale isolation of plasmid was performed using a mini-DNA preparation kit (QIAGEN).

Article Title: qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin ▿
Article Snippet: .. Competent Escherichia coli cells were used in the transformation and cloning experiments: TG1 cells (Stratagene, Cambridge, United Kingdom) were used in the initial transformation experiments with the natural plasmid and cloned plasmid fragment, and DH10B cells (Invitrogen, Cergy Pontoise, France) were used for transformation with the native plasmid and vector pBR322, containing the resistance genes qnrD , qnrA1 , and qnrS1 . .. Plasmids pACYC177 (New England Biolabs, Hitchin, United Kingdom), containing resistance to ampicillin and kanamycin, and pBR322 (New England Biolabs, Hitchin, United Kingdom), containing resistance to tetracycline and ampicillin, were used in the cloning of plasmid restriction fragments and PCR products.

Plasmid Preparation:

Article Title: Characterization of the Enzymatic Component of the ADP-Ribosyltransferase Toxin CDTa from Clostridium difficile
Article Snippet: .. Oligonucleotides were purchased from MWG (Ebersberg, Germany), the pGEX2T vector (included in the glutathione S -transferase [GST] Gene Fusion System) and glutathione Sepharose 4B were obtained from Pharmacia Biotech (Uppsala, Sweden), the DNA molecular weight marker (lambda Hin dIII) and restriction enzymes were from Roche Diagnostics (Mannheim, Germany), and T4 ligase and competent E. coli cells were from Stratagene (Heidelberg, Germany). .. The Topo-TA-Cloning kit was from Invitrogen (Groningen, The Netherlands).

Article Title: Cholinergic signaling inhibits oxalate transport by human intestinal T84 cells
Article Snippet: .. Transfection-grade plasmid DNA was prepared by transforming these plasmids into competent Escherichia coli cells following Stratagene's protocol. .. To accomplish SLC26A6 knockdown in T84 cells, 2 × 106 cells were untransfected (UT) or nucleofected with 12 μg of shRNA plasmid DNA (NC = negative control shRNA or S1 and S2 = shRNAs targeting SLC26A6) using the Cell Line Nucleofector Kit T (Amaxa: catalog no. VCA-1002; Program T-005).

Article Title: qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin ▿
Article Snippet: .. Competent Escherichia coli cells were used in the transformation and cloning experiments: TG1 cells (Stratagene, Cambridge, United Kingdom) were used in the initial transformation experiments with the natural plasmid and cloned plasmid fragment, and DH10B cells (Invitrogen, Cergy Pontoise, France) were used for transformation with the native plasmid and vector pBR322, containing the resistance genes qnrD , qnrA1 , and qnrS1 . .. Plasmids pACYC177 (New England Biolabs, Hitchin, United Kingdom), containing resistance to ampicillin and kanamycin, and pBR322 (New England Biolabs, Hitchin, United Kingdom), containing resistance to tetracycline and ampicillin, were used in the cloning of plasmid restriction fragments and PCR products.

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