competent e coli bacteria  (Thermo Fisher)


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    Name:
    One Shot TOP10 Chemically Competent E coli
    Description:
    One Shot TOP10 Chemically Competent E coli are provided at a transformation efficiency of 1 x 109 cfu µg plasmid DNA and are ideal for high efficiency cloning and plasmid propagation They allow stable replication of high copy number plasmids and are the same competent cells that come with many of our cloning kits Features of One Shot TOP10 cells include • Maximization of cloning efficiency in a single tube format• Enhanced genomic DNA cloning capabilitiesEasy to use One Shot formatThe single tube single use format allows for all steps of the transformation protocol up to plating to take place in the same tube thereby helping to save time and prevent contamination Versatile cloning capabilitiesOne Shot TOP10 E coli cells are similar to the DH10B strain and offer the following features • hsdR for efficient transformation of unmethylated DNA from PCR amplifications• mcrA for efficient transformation of methylated DNA from genomic preparations• lacZΔM15 for blue white color screening of recombinant clones• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I• recA1 for reduced occurrence of nonspecific recombination in cloned DNAGenotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ araleu 7697 galU galK rpsL StrR endA1 nupGFind the strain and format that you needWe also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs If you require high throughput transformation choose from our collection of MultiShot formatted comp cells
    Catalog Number:
    c404003
    Price:
    None
    Applications:
    Chemically Competent Cells for Cloning|Cloning|Transformation
    Category:
    Competent Cells Strains
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    Structured Review

    Thermo Fisher competent e coli bacteria
    One Shot TOP10 Chemically Competent E coli are provided at a transformation efficiency of 1 x 109 cfu µg plasmid DNA and are ideal for high efficiency cloning and plasmid propagation They allow stable replication of high copy number plasmids and are the same competent cells that come with many of our cloning kits Features of One Shot TOP10 cells include • Maximization of cloning efficiency in a single tube format• Enhanced genomic DNA cloning capabilitiesEasy to use One Shot formatThe single tube single use format allows for all steps of the transformation protocol up to plating to take place in the same tube thereby helping to save time and prevent contamination Versatile cloning capabilitiesOne Shot TOP10 E coli cells are similar to the DH10B strain and offer the following features • hsdR for efficient transformation of unmethylated DNA from PCR amplifications• mcrA for efficient transformation of methylated DNA from genomic preparations• lacZΔM15 for blue white color screening of recombinant clones• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I• recA1 for reduced occurrence of nonspecific recombination in cloned DNAGenotype F mcrA Δ mrr hsdRMS mcrBC Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ araleu 7697 galU galK rpsL StrR endA1 nupGFind the strain and format that you needWe also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs If you require high throughput transformation choose from our collection of MultiShot formatted comp cells
    https://www.bioz.com/result/competent e coli bacteria/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    competent e coli bacteria - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach
    Article Snippet: .. E . coli strains The following commercially available strains of E . coli were also used for AQUA Cloning: One Shot TOP10 (Invitrogen cat. No. C4040-03, competency: > 109 CFU/μg), NEB5α (NEB, cat. No. C2987I, competency: 1–3 x 109 CFU/μg), NEB10β (NEB, cat. No. C3019I, competency: 1–3 x 109 CFU/μg), BL21 (DE3) (NEB, cat. No. C2527I, competency: 1–5 x 107 CFU/μg), JM109 (Promega, cat. No. L2005, competency: 108 CFU/μg). .. Confocal imaging For confocal imaging, human embryonic kidney cells (HEK-293T) [ ], or NIH/3T3 fibroblasts cells (ATCC CRL-1658) were maintained in Dulbecco’s modified Eagle’s medium (PAN, cat. No. P03-0710) supplemented with 10% fetal bovine serum (PAN, cat. no. 1502-P123002, batch no. P123002), 100 U/mL penicillin and 0.1 mg/mL streptomycin (PAN).

    Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
    Article Snippet: .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03). .. Kanamycin-resistant colonies were selected and plasmid DNA was prepared using the QIAprep Spin Miniprep Kit (Qiagen, 27104).

    Article Title: Predicting RNA hyper-editing with a novel tool when unambiguous alignment is impossible
    Article Snippet: .. Subsequently, 4 μ L of PCR product was cloned into One Shot®;TOP10 Chemically Competent E. coli cells using Zero Blunt®;TOPO®;PCR Cloning Kit from Invitrogen (catalog number: K2800J10), according to manufacturer’s guidelines. .. A total of 50 μ L solution containing the transformed cells were plated on kanamycin+agar plates.

    other:

    Article Title: Monoubiquitination of ASXLs controls the deubiquitinase activity of the tumor suppressor BAP1
    Article Snippet: Bacterial strains TOP10 chemically competent E.Coli bacteria (One Shot™ TOP10 Chemically Competent E. coli) were purchased from ThermoFisher (C404010).

    DNA Sequencing:

    Article Title: The polypeptide Syn67 interacts physically with human holocarboxylase synthetase, but is not a target for biotinylation
    Article Snippet: .. Eluted plasmids (10 µL) were used to transform One Shot TOP10 chemically competent E. coli cells (Invitrogen, Carlsbad, CA) for subsequent plasmid preparation (QIAprep Spin Miniprep kit, Qiagen, Valencia, CA) and DNA sequencing. ..

    Polymerase Chain Reaction:

    Article Title: Predicting RNA hyper-editing with a novel tool when unambiguous alignment is impossible
    Article Snippet: .. Subsequently, 4 μ L of PCR product was cloned into One Shot®;TOP10 Chemically Competent E. coli cells using Zero Blunt®;TOPO®;PCR Cloning Kit from Invitrogen (catalog number: K2800J10), according to manufacturer’s guidelines. .. A total of 50 μ L solution containing the transformed cells were plated on kanamycin+agar plates.

    Transformation Assay:

    Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
    Article Snippet: .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03). .. Kanamycin-resistant colonies were selected and plasmid DNA was prepared using the QIAprep Spin Miniprep Kit (Qiagen, 27104).

    Article Title: Mössbauer Study and Modeling of Iron Import and Trafficking in Human Jurkat Cells
    Article Snippet: .. The resulting plasmid was transformed into OneShot TOP10 E. coli competent cells (Life Technologies), which were then plated and grown at 37 °C overnight on LB agar plates containing 100 μg/mL of ampicillin. .. Plasmids were purified from these E. coli cells using the PureLink Quick Plasmid Miniprep kit (Life Technologies) as per manufacturer's instructions.

    Article Title: Quorum-Sensing Systems LuxS/Autoinducer 2 and Com Regulate Streptococcus pneumoniae Biofilms in a Bioreactor with Living Cultures of Human Respiratory Cells
    Article Snippet: .. This gene was ligated into a previously KpnI-XbaI-digested pPP2 integrative plasmid ( ) using T4 DNA ligase (Promega) and transformed into One Shot TOP10 chemically competent Escherichia coli (Invitrogen). .. Transformants were selected on LB agar plates containing 100 μg/ml ampicillin and screened by colony PCR with primers comC-L and comC-R, and comC -bearing clones were confirmed by sequencing.

    Plasmid Preparation:

    Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
    Article Snippet: .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03). .. Kanamycin-resistant colonies were selected and plasmid DNA was prepared using the QIAprep Spin Miniprep Kit (Qiagen, 27104).

    Article Title: Mössbauer Study and Modeling of Iron Import and Trafficking in Human Jurkat Cells
    Article Snippet: .. The resulting plasmid was transformed into OneShot TOP10 E. coli competent cells (Life Technologies), which were then plated and grown at 37 °C overnight on LB agar plates containing 100 μg/mL of ampicillin. .. Plasmids were purified from these E. coli cells using the PureLink Quick Plasmid Miniprep kit (Life Technologies) as per manufacturer's instructions.

    Article Title: The polypeptide Syn67 interacts physically with human holocarboxylase synthetase, but is not a target for biotinylation
    Article Snippet: .. Eluted plasmids (10 µL) were used to transform One Shot TOP10 chemically competent E. coli cells (Invitrogen, Carlsbad, CA) for subsequent plasmid preparation (QIAprep Spin Miniprep kit, Qiagen, Valencia, CA) and DNA sequencing. ..

    Article Title: Quorum-Sensing Systems LuxS/Autoinducer 2 and Com Regulate Streptococcus pneumoniae Biofilms in a Bioreactor with Living Cultures of Human Respiratory Cells
    Article Snippet: .. This gene was ligated into a previously KpnI-XbaI-digested pPP2 integrative plasmid ( ) using T4 DNA ligase (Promega) and transformed into One Shot TOP10 chemically competent Escherichia coli (Invitrogen). .. Transformants were selected on LB agar plates containing 100 μg/ml ampicillin and screened by colony PCR with primers comC-L and comC-R, and comC -bearing clones were confirmed by sequencing.

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  • 96
    Thermo Fisher competent e coli bacteria
    Competent E Coli Bacteria, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli bacteria/product/Thermo Fisher
    Average 96 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    competent e coli bacteria - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

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