Structured Review

Dr Maisch HPLC column material reprosil pur
Column Material Reprosil Pur, supplied by Dr Maisch HPLC, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/column material reprosil pur/product/Dr Maisch HPLC
Average 87 stars, based on 2 article reviews
Price from $9.99 to $1999.99
column material reprosil pur - by Bioz Stars, 2020-02
87/100 stars

Related Products / Commonly Used Together

c18
orbitrap fusion instrument
easy-nlc 1000

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Related Articles

Modification:

Article Title: Memory B Cells Activate Brain-Homing, Autoreactive CD4+ T Cells in Multiple Sclerosis
Article Snippet: Resulting peptides were dissolved in 3% acetonitrile and 0.1% formic acid before injecting into an Easy-nLC 1000 linked to an Orbitrap Fusion instrument (Thermo Fisher Scientific) on a gradient of 120 min. As column material ReproSil-Pur, C18, 120 Å, AQ, 1.9 μm (Dr. Maisch GmbH) with column dimensions ID 0.075mm/ length 150mm was used. .. Carbamidomethyl at cysteine was set as a static modification, and oxidation of methionine, acetylation of lysine and protein N terminus were set as variable modification.

Sample Prep:

Article Title: Memory B Cells Activate Brain-Homing, Autoreactive CD4+ T Cells in Multiple Sclerosis
Article Snippet: Proteomics by LC-MS/MS In order to obtain peptides for mass spectrometric analysis, dry pellets of magnetically sorted untouched CD19+ B cells (HD and MS (REM), each n = 4; samples sizes of 2-6x106 cells) were lysed with an adapted filter aided sample preparation (FASP) protocol as previously described ( ). .. Resulting peptides were dissolved in 3% acetonitrile and 0.1% formic acid before injecting into an Easy-nLC 1000 linked to an Orbitrap Fusion instrument (Thermo Fisher Scientific) on a gradient of 120 min. As column material ReproSil-Pur, C18, 120 Å, AQ, 1.9 μm (Dr. Maisch GmbH) with column dimensions ID 0.075mm/ length 150mm was used.

Mass Spectrometry:

Article Title: Memory B Cells Activate Brain-Homing, Autoreactive CD4+ T Cells in Multiple Sclerosis
Article Snippet: Fresh frozen brain tissue from cortical gray matter from brains (n = 6 controls, pooled and n = 6 MS, pooled) was dissociated and lysed using a PCT technology as previously described ( ). .. Resulting peptides were dissolved in 3% acetonitrile and 0.1% formic acid before injecting into an Easy-nLC 1000 linked to an Orbitrap Fusion instrument (Thermo Fisher Scientific) on a gradient of 120 min. As column material ReproSil-Pur, C18, 120 Å, AQ, 1.9 μm (Dr. Maisch GmbH) with column dimensions ID 0.075mm/ length 150mm was used.

Software:

Article Title: Memory B Cells Activate Brain-Homing, Autoreactive CD4+ T Cells in Multiple Sclerosis
Article Snippet: Resulting peptides were dissolved in 3% acetonitrile and 0.1% formic acid before injecting into an Easy-nLC 1000 linked to an Orbitrap Fusion instrument (Thermo Fisher Scientific) on a gradient of 120 min. As column material ReproSil-Pur, C18, 120 Å, AQ, 1.9 μm (Dr. Maisch GmbH) with column dimensions ID 0.075mm/ length 150mm was used. .. For protein identification, “raw” files were converted into “mgf” files and analyzed on MASCOT software with a human UniProtKB/Swiss-Prot protein database (date: March 22, 2016 with 40912 entries).

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    Dr Maisch HPLC column c18 material
    Column C18 Material, supplied by Dr Maisch HPLC, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/column c18 material/product/Dr Maisch HPLC
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    column c18 material - by Bioz Stars, 2020-02
    78/100 stars
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