colorimetric warmstart lamp 2x master mix  (New England Biolabs)


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    New England Biolabs colorimetric warmstart lamp 2x master mix
    Results of the <t>WarmStart</t> Colorimetric LAMP 2X Master Mix assay for detection of LAMP amplicons with the naked eye. Samples 1–8: serial dilutions of DNA from strain G. ( H.) parasuis DSM 21448 starting at concentrations of 10 ng/µL up to 1 fg/µL. Sample 9: DNA from Actinobacillus minor CCUG 38923 T . Sample 10: no template control.
    Colorimetric Warmstart Lamp 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/colorimetric warmstart lamp 2x master mix/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    colorimetric warmstart lamp 2x master mix - by Bioz Stars, 2022-07
    97/100 stars

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    1) Product Images from "Development and Validation of a Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Glaesserella (Haemophilus) parasuis"

    Article Title: Development and Validation of a Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Glaesserella (Haemophilus) parasuis

    Journal: Microorganisms

    doi: 10.3390/microorganisms9010041

    Results of the WarmStart Colorimetric LAMP 2X Master Mix assay for detection of LAMP amplicons with the naked eye. Samples 1–8: serial dilutions of DNA from strain G. ( H.) parasuis DSM 21448 starting at concentrations of 10 ng/µL up to 1 fg/µL. Sample 9: DNA from Actinobacillus minor CCUG 38923 T . Sample 10: no template control.
    Figure Legend Snippet: Results of the WarmStart Colorimetric LAMP 2X Master Mix assay for detection of LAMP amplicons with the naked eye. Samples 1–8: serial dilutions of DNA from strain G. ( H.) parasuis DSM 21448 starting at concentrations of 10 ng/µL up to 1 fg/µL. Sample 9: DNA from Actinobacillus minor CCUG 38923 T . Sample 10: no template control.

    Techniques Used:

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    New England Biolabs colorimetric warmstart lamp 2x master mix
    Results of the <t>WarmStart</t> Colorimetric LAMP 2X Master Mix assay for detection of LAMP amplicons with the naked eye. Samples 1–8: serial dilutions of DNA from strain G. ( H.) parasuis DSM 21448 starting at concentrations of 10 ng/µL up to 1 fg/µL. Sample 9: DNA from Actinobacillus minor CCUG 38923 T . Sample 10: no template control.
    Colorimetric Warmstart Lamp 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/colorimetric warmstart lamp 2x master mix/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    colorimetric warmstart lamp 2x master mix - by Bioz Stars, 2022-07
    97/100 stars
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    Results of the WarmStart Colorimetric LAMP 2X Master Mix assay for detection of LAMP amplicons with the naked eye. Samples 1–8: serial dilutions of DNA from strain G. ( H.) parasuis DSM 21448 starting at concentrations of 10 ng/µL up to 1 fg/µL. Sample 9: DNA from Actinobacillus minor CCUG 38923 T . Sample 10: no template control.

    Journal: Microorganisms

    Article Title: Development and Validation of a Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Glaesserella (Haemophilus) parasuis

    doi: 10.3390/microorganisms9010041

    Figure Lengend Snippet: Results of the WarmStart Colorimetric LAMP 2X Master Mix assay for detection of LAMP amplicons with the naked eye. Samples 1–8: serial dilutions of DNA from strain G. ( H.) parasuis DSM 21448 starting at concentrations of 10 ng/µL up to 1 fg/µL. Sample 9: DNA from Actinobacillus minor CCUG 38923 T . Sample 10: no template control.

    Article Snippet: The isothermal OptiGene Isothermal Master Mix was replaced by the colorimetric WarmStart LAMP 2X Master Mix (New England BioLabs, Frankfurt am Main, Germany).

    Techniques:

    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Journal: Biosensors & bioelectronics

    Article Title: Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics

    doi: 10.1016/j.bios.2018.03.009

    Figure Lengend Snippet: Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Article Snippet: Bovine serum albumin (BSA), Poly (ethylene glycol) 8000 (PEG), poly(vinyl alcohol) (PVA) are from Sigma-Aldrich, RT-PCR grade water from Ambion, Inc., intercalating Eva Green fluorescent dye from Biotium, Isothermal Master Mix used in LAMP reaction buffer from ISO-001nd, OptiGene, Horsham, UK, WarmStart® colorimetric LAMP 2X master mix from New England Biolabs Inc. N. meningitidis (ATCC 13098) from American Type Culture Collection (ATCC, Rockville, MD).

    Techniques: Fluorescence, Lamp Assay, Chromatin Immunoprecipitation, Amplification, Concentration Assay

    Effect of primer multiplexing and supplements. (A) Evaluation of RT-LAMP performance with the indicated primer multiplexing. RT-LAMP reactions were carried out with 15 copies of SARS-CoV-2 RNA at the optimized concentration for each primer set (see Fig 2A in bold). (B) Evaluation of RT-LAMP performance with 40mM GuHCl and/or 0.5M betaine. Reactions were performed with multiplexed Gene E1 and ORF1a primers. (C) LoD assessment of the best RT-LAMP condition with the indicated copy numbers of SARS-CoV-2 RNA. (D) Fluorescent readouts and color changes of the reactions in (C) at 60 minutes. Each condition was evaluated with 10 replicates. NTC, no template control; TTR, time to results; RFU, relative fluorescent units; Error bars represent mean ± standard deviations.

    Journal: PLoS ONE

    Article Title: Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application

    doi: 10.1371/journal.pone.0268340

    Figure Lengend Snippet: Effect of primer multiplexing and supplements. (A) Evaluation of RT-LAMP performance with the indicated primer multiplexing. RT-LAMP reactions were carried out with 15 copies of SARS-CoV-2 RNA at the optimized concentration for each primer set (see Fig 2A in bold). (B) Evaluation of RT-LAMP performance with 40mM GuHCl and/or 0.5M betaine. Reactions were performed with multiplexed Gene E1 and ORF1a primers. (C) LoD assessment of the best RT-LAMP condition with the indicated copy numbers of SARS-CoV-2 RNA. (D) Fluorescent readouts and color changes of the reactions in (C) at 60 minutes. Each condition was evaluated with 10 replicates. NTC, no template control; TTR, time to results; RFU, relative fluorescent units; Error bars represent mean ± standard deviations.

    Article Snippet: The volume for each RT-LAMP reaction was 10μl, including 5μl WarmStart colorimetric LAMP 2X Master Mix, 1μl 10X primer set stock and 1μl template.

    Techniques: Multiplexing, Concentration Assay

    Screening primer performance at a low copy number of SARS-CoV-2 RNA. (A) A schematic showing a DNA template amplified by LAMP and the primers targeted to the regions in the template. (B) Location of the 7 target regions for the 14 primer sets in the SARS-CoV-2 genome (NC_045512.2, [ 34 ]). The indicated target region is that amplified by the outer F3 and B3 primers. (C) Matrix of test conditions. Each primer set was tested with the indicated primer molar ratio (black), and primer concentrations (blue). A total of 16 conditions were tested for each of the 14 primer sets, with 4 replicates per condition. Other reaction reagents are indicated in red. (D) Screening results for primer Gene E1. Top panel: For the indicated primer mixes (X-axis), red and blue bars indicate TTR using 30 copies of positive control SARS-CoV-2 RNA (TTR PC ) or no template (TTR NTC ), respectively. Red and blue circles indicate sensitivity and specificity, respectively. Bottom left graph shows an example of the fluorescent signal obtained with STYO 9 dye over the 60 minute reaction period for PC (red) or NTC (blue–undetected) using the indicated Gene E1 primer mix. Green line: Threshold to designate TTR. Bottom right panel shows an example of the phenol red colour at 60 minutes. (E and F) Screening results of primer ORF1a (E) and human ACTB (F); format as in (D). (G) Summary of the best two primer concentrations for the top performing four primer sets with adequate performance based on sensitivity, specificity and TTR. NTC, no template control; PC, positive control (30 copies of SARS-CoV-2 RNA); Sensitivity, the percentage of PC replicates with amplifications; Specificity, the percentage of NTC replicates without amplifications; RFU, relative fluorescence units; TTR, time to results (minutes), the time point that the RFU curve crossing the fluorescent threshold; Error bars represent mean ± standard deviations.

    Journal: PLoS ONE

    Article Title: Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application

    doi: 10.1371/journal.pone.0268340

    Figure Lengend Snippet: Screening primer performance at a low copy number of SARS-CoV-2 RNA. (A) A schematic showing a DNA template amplified by LAMP and the primers targeted to the regions in the template. (B) Location of the 7 target regions for the 14 primer sets in the SARS-CoV-2 genome (NC_045512.2, [ 34 ]). The indicated target region is that amplified by the outer F3 and B3 primers. (C) Matrix of test conditions. Each primer set was tested with the indicated primer molar ratio (black), and primer concentrations (blue). A total of 16 conditions were tested for each of the 14 primer sets, with 4 replicates per condition. Other reaction reagents are indicated in red. (D) Screening results for primer Gene E1. Top panel: For the indicated primer mixes (X-axis), red and blue bars indicate TTR using 30 copies of positive control SARS-CoV-2 RNA (TTR PC ) or no template (TTR NTC ), respectively. Red and blue circles indicate sensitivity and specificity, respectively. Bottom left graph shows an example of the fluorescent signal obtained with STYO 9 dye over the 60 minute reaction period for PC (red) or NTC (blue–undetected) using the indicated Gene E1 primer mix. Green line: Threshold to designate TTR. Bottom right panel shows an example of the phenol red colour at 60 minutes. (E and F) Screening results of primer ORF1a (E) and human ACTB (F); format as in (D). (G) Summary of the best two primer concentrations for the top performing four primer sets with adequate performance based on sensitivity, specificity and TTR. NTC, no template control; PC, positive control (30 copies of SARS-CoV-2 RNA); Sensitivity, the percentage of PC replicates with amplifications; Specificity, the percentage of NTC replicates without amplifications; RFU, relative fluorescence units; TTR, time to results (minutes), the time point that the RFU curve crossing the fluorescent threshold; Error bars represent mean ± standard deviations.

    Article Snippet: The volume for each RT-LAMP reaction was 10μl, including 5μl WarmStart colorimetric LAMP 2X Master Mix, 1μl 10X primer set stock and 1μl template.

    Techniques: Low Copy Number, Amplification, Positive Control, Fluorescence

    Evaluation of the optimized primer concentrations based on limit of detection and specificity. (A) ORF1a, Gene E1, Gene N2 and N-gene primers were assessed at the indicated conditions. Each condition was evaluated with 10 replicates. (B) ACTB primers were evaluated under the indicated conditions. Sensitivity, the percentage of replicates with SARS-CoV-2 RNA or human RNA showing amplifications; Specificity, the percentage of no template controls without amplifications; TTR, time to results (minutes); LoD, limit of detection; Error bars represent mean ± standard deviations.

    Journal: PLoS ONE

    Article Title: Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application

    doi: 10.1371/journal.pone.0268340

    Figure Lengend Snippet: Evaluation of the optimized primer concentrations based on limit of detection and specificity. (A) ORF1a, Gene E1, Gene N2 and N-gene primers were assessed at the indicated conditions. Each condition was evaluated with 10 replicates. (B) ACTB primers were evaluated under the indicated conditions. Sensitivity, the percentage of replicates with SARS-CoV-2 RNA or human RNA showing amplifications; Specificity, the percentage of no template controls without amplifications; TTR, time to results (minutes); LoD, limit of detection; Error bars represent mean ± standard deviations.

    Article Snippet: The volume for each RT-LAMP reaction was 10μl, including 5μl WarmStart colorimetric LAMP 2X Master Mix, 1μl 10X primer set stock and 1μl template.

    Techniques:

    Direct RT-LAMP on raw clinical NP samples without RNA extraction. (A) ROC curves evaluating RT-LAMP performance on 30 positive and 36 negative clinical NP samples with the indicated supplements. 0.5M betaine + 0.25% Igepal CA-630 in green; 0.5M betaine in red; No supplements in pink; 40mM GuHCl + 0.5M betaine in light blue; 40mM GuHCl in black. 1μl of raw samples (without any sample processing) was applied to RT-LAMP reactions, and the reactions were carried out with multiplexing primers for Gene E1 and ORF1a. Significance values were calculated with MedCalc software for ROC curve analysis. TTR* indicates the cutoff providing optimal sensitivity and specificity. (B) Distribution of the RT-LAMP TTRs vs . BGI RT-PCR Ct values with the indicated supplements. Dotted lines indicate cutoffs. (C) Representative fluorescent readouts of RT-LAMP with 0.5M betaine and 0.25% Igepal CA-630. (D) Sensitivity of RT-LAMP at the indicated Ct ranges. Left panel, Clinical NP samples. Right panel, Contrived positives generated by diluting clinical NP positives with negative NP samples.

    Journal: PLoS ONE

    Article Title: Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application

    doi: 10.1371/journal.pone.0268340

    Figure Lengend Snippet: Direct RT-LAMP on raw clinical NP samples without RNA extraction. (A) ROC curves evaluating RT-LAMP performance on 30 positive and 36 negative clinical NP samples with the indicated supplements. 0.5M betaine + 0.25% Igepal CA-630 in green; 0.5M betaine in red; No supplements in pink; 40mM GuHCl + 0.5M betaine in light blue; 40mM GuHCl in black. 1μl of raw samples (without any sample processing) was applied to RT-LAMP reactions, and the reactions were carried out with multiplexing primers for Gene E1 and ORF1a. Significance values were calculated with MedCalc software for ROC curve analysis. TTR* indicates the cutoff providing optimal sensitivity and specificity. (B) Distribution of the RT-LAMP TTRs vs . BGI RT-PCR Ct values with the indicated supplements. Dotted lines indicate cutoffs. (C) Representative fluorescent readouts of RT-LAMP with 0.5M betaine and 0.25% Igepal CA-630. (D) Sensitivity of RT-LAMP at the indicated Ct ranges. Left panel, Clinical NP samples. Right panel, Contrived positives generated by diluting clinical NP positives with negative NP samples.

    Article Snippet: The volume for each RT-LAMP reaction was 10μl, including 5μl WarmStart colorimetric LAMP 2X Master Mix, 1μl 10X primer set stock and 1μl template.

    Techniques: RNA Extraction, Multiplexing, Software, Reverse Transcription Polymerase Chain Reaction, Generated

    Comparison of RT-LAMP and BGI RT-PCR with extracted RNA from clinical NP samples. (A) Correlation of Ct values with BGI RT-PCR kit vs . other indicated RT-PCR reagents in 30 SARS-CoV-2 positive clinical NP samples. (B) ROC curve evaluating RT-LAMP performance with 30 positive and 36 negative Canadian clinical samples based on the results of BGI RT-PCR kit. TPR: True positive rate; FPR: False positive rate. TTR ≤ 13.2’ was defined as the cut-off to distinguish positive from negative samples with 90% detection sensitivity and 100% specificity. (C) Distribution of RT-LAMP TTRs against BGI RT-PCR Ct values for 30 positive and 36 negative clinical NP samples. BGI RT-PCR and RT-LAMP positives were defined by Ct

    Journal: PLoS ONE

    Article Title: Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application

    doi: 10.1371/journal.pone.0268340

    Figure Lengend Snippet: Comparison of RT-LAMP and BGI RT-PCR with extracted RNA from clinical NP samples. (A) Correlation of Ct values with BGI RT-PCR kit vs . other indicated RT-PCR reagents in 30 SARS-CoV-2 positive clinical NP samples. (B) ROC curve evaluating RT-LAMP performance with 30 positive and 36 negative Canadian clinical samples based on the results of BGI RT-PCR kit. TPR: True positive rate; FPR: False positive rate. TTR ≤ 13.2’ was defined as the cut-off to distinguish positive from negative samples with 90% detection sensitivity and 100% specificity. (C) Distribution of RT-LAMP TTRs against BGI RT-PCR Ct values for 30 positive and 36 negative clinical NP samples. BGI RT-PCR and RT-LAMP positives were defined by Ct

    Article Snippet: The volume for each RT-LAMP reaction was 10μl, including 5μl WarmStart colorimetric LAMP 2X Master Mix, 1μl 10X primer set stock and 1μl template.

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Receiver operating characteristic curve (ROC curve) to study the performance of the newly developed LAMP assay for the detection of SARS CoV-2.

    Journal: Journal of Clinical Virology plus

    Article Title: Diagnostic utility and validation of a newly developed real time loop mediated isothermal amplification method for the detection of SARS CoV-2 infection

    doi: 10.1016/j.jcvp.2022.100081

    Figure Lengend Snippet: Receiver operating characteristic curve (ROC curve) to study the performance of the newly developed LAMP assay for the detection of SARS CoV-2.

    Article Snippet: The assay was performed in a 25 μL LAMP reaction mixture containing 2.5μLof 10X LAMP primer mix (16 μM F1P and B1P, 4μM LPF and LPB, 2 μMF3 and B3), 12.5 μL of Warm Start LAMP 2X Master mix, 1.5 μL of DMSO, 3 μL of nuclease free water and 0.5μL of 50X fluorescence dye supplied by the WarmStart LAMP kit and 5 μL of template.

    Techniques: Lamp Assay

    Comparison of turnaround time for real time RT-LAMP and real time RT-PCR for the detection of SARS CoV-2.

    Journal: Journal of Clinical Virology plus

    Article Title: Diagnostic utility and validation of a newly developed real time loop mediated isothermal amplification method for the detection of SARS CoV-2 infection

    doi: 10.1016/j.jcvp.2022.100081

    Figure Lengend Snippet: Comparison of turnaround time for real time RT-LAMP and real time RT-PCR for the detection of SARS CoV-2.

    Article Snippet: The assay was performed in a 25 μL LAMP reaction mixture containing 2.5μLof 10X LAMP primer mix (16 μM F1P and B1P, 4μM LPF and LPB, 2 μMF3 and B3), 12.5 μL of Warm Start LAMP 2X Master mix, 1.5 μL of DMSO, 3 μL of nuclease free water and 0.5μL of 50X fluorescence dye supplied by the WarmStart LAMP kit and 5 μL of template.

    Techniques: Quantitative RT-PCR

    SARS-CoV-2 RNA detected by direct visualization for the presence and absence of turbidity. A - Positive samples showing turbidity . B - Negative samples not showing turbidity.

    Journal: Journal of Clinical Virology plus

    Article Title: Diagnostic utility and validation of a newly developed real time loop mediated isothermal amplification method for the detection of SARS CoV-2 infection

    doi: 10.1016/j.jcvp.2022.100081

    Figure Lengend Snippet: SARS-CoV-2 RNA detected by direct visualization for the presence and absence of turbidity. A - Positive samples showing turbidity . B - Negative samples not showing turbidity.

    Article Snippet: The assay was performed in a 25 μL LAMP reaction mixture containing 2.5μLof 10X LAMP primer mix (16 μM F1P and B1P, 4μM LPF and LPB, 2 μMF3 and B3), 12.5 μL of Warm Start LAMP 2X Master mix, 1.5 μL of DMSO, 3 μL of nuclease free water and 0.5μL of 50X fluorescence dye supplied by the WarmStart LAMP kit and 5 μL of template.

    Techniques: