collagenase  (Roche)


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    Structured Review

    Roche collagenase
    Collagenase, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase - by Bioz Stars, 2021-04
    86/100 stars

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    Article Title: Resveratrol Rescue Indoxyl Sulfate-Induced Deterioration of Osteoblastogenesis via the Aryl Hydrocarbon Receptor /MAPK Pathway
    Article Snippet: The dissected calvariae were rinsed in phosphatase-buffered saline (PBS) from GE Healthcare Life Science (Marlborough, MA, USA) and soaked with 1 mL of 0.05% trypsin from Corning Life Sciences (Union City, CA, USA)/0.1 mM EDTA from Omics Bio (Taichung City, Taiwan) containing 5 mg collagenase/Collagenase P from Roche Applied Science (Indianapolis, IN, USA) 10 times for 10 min each.

    Article Title: Expanding Mouse Ventricular Cardiomyocytes through GSK-3 Inhibition
    Article Snippet: Phosphate Buffered Saline (PBS) 1x, Collagenase A (Roche, Cat. No. 11 088 785 103) 1mg/mL, Collagenase B (Roche, Cat. No. 11 088 823 103) 1mg/mL and 20% Fetal Bovine Serum (FBS) (Gemini Bioproducts)

    Article Title: Fate mapping reveals that microglia and recruited monocyte-derived macrophages are definitively distinguishable by phenotype in the retina
    Article Snippet: Collagenase digestion Single retinas were digested in 1.5 mg/ml collagenase A and 0.4 mg/ml DNase I (Roche, Indianapolis, IN) for 1 hour at 37 °C with agitation.

    Article Title: The Xenopus laevis Isoform of G Protein-Coupled Receptor 3 (GPR3) Is a Constitutively Active Cell Surface Receptor that Participates in Maintaining Meiotic Arrest in X. laevis Oocytes
    Article Snippet: For the cell surface assays, COS-7 cells were treated with either serum free media alone or serum free media containing 0.8 mg/ml collagenase A (Roche Applied Science) for 30 min.

    Purification:

    Article Title: Improved yield of canine islet isolation from deceased donors
    Article Snippet: .. Collagenase solution A highly purified collagenase/thermolysin blend, Liberase T-Flex, (Roche Custom Biotech, Indianapolis, IN) was dissolved in the above-described HBSS digestion buffer. ..

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  • 86
    Roche collagenase dispase
    CCL25 RNA is expressed in thymic endothelial cells and is also highly expressed in hyperreceptive thymi of IL-7R −/− and PSGL-1 −/− mice. (a) RNA levels of ICAM-1, VCAM-1, and CCL25 in the thymi of WT and RAG-1 −/− , C2GnT1 −/− , PSGL-1 −/− , IL-7R −/− , and Psel −/− mice, as determined by qrtPCR. RNA levels were normalized to VE-cadherin and HPRT expression and expressed relative to WT (=1, as indicated). (b) Relative expression of the indicated genes in FACS-sorted WT thymic endothelial cells (ENDO; CD45 − , CD31 + , CD144 + ), cortical epithelial cells (CEC; CD45 − , Ly51 hi , G8.8 + ), and medullary epithelial cells (MEC; CD45 − , Ly51 int , G8.8 + ) relative to expression in an unsorted sample. RNA levels were normalized to the reference genes HPRT and TbP. N.D., not detected. (c) Numbers of ENDO, CEC, and MEC in the thymi of the indicated mouse strains. For b and c, thymi were sequentially digested with collagenase and <t>collagenase/dispase,</t> and single-cell suspensions were analyzed by flow cytometry using reference beads to calculate absolute cell numbers. ENDO, CEC, and MEC were defined as in b. Data are representative of at least three independent experiments with at least five mice in a and c, and four mice in b (means ± SEM). Mice were sex matched and 35 ± 4 d old. C2, C2GnT1. *, P
    Collagenase Dispase, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase dispase/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase dispase - by Bioz Stars, 2021-04
    86/100 stars
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    86
    Roche collagenase d
    Absence of donor APC-derived IL-23 significantly attenuates proinflammatory cytokine production in the colon . (A,B) Lethally irradiated (900 cGy) Balb/c mice were transplanted with B6 BM (10 × 10 6 ) and spleen cells (0.4-0.6 × 10 6 ) (■) or IL-23 −/−  BM (10 × 10 6 ) and spleen cells (□) adjusted to yield an equivalent number of mature T cells. Cohorts of mice (7-9/group) were killed at the indicated time points (days 4, 7, 14, 21, and 28), and segments of colon tissue from individual mice were cultured overnight in media. Colonic tissue supernatants were collected and analyzed for the amount of IL-23p19 (A) and IL-12p70 (B) by ELISA and multiplex, respectively. Data are presented as mean amount of cytokine divided by the weight of cultured colon tissue (± SEM) and are cumulative results from 2 experiments. (C) Groups of mice were transplanted as in panels A and B and killed 28 days posttransplantation. Colons (n = 4-5/group per experiment) were pooled and digested with collagenase D. Total number of isolated LPMCs that were CD11c +  CD11b +  or CD11b +  CD11c −  in Balb/c recipients of B6 BM and spleen cells (■) or IL-23 −/−  BM and spleen cells (□) is depicted. Data are derived from 3 independent experiments and are presented as the mean cell number (×1000) per mouse (± SEM) (D). Lethally irradiated Balb/c mice were transplanted with B6 BM plus spleen cells (n = 10, ■) or IL-23 −/−  BM and spleen cells adjusted to yield equivalent numbers of T cells (n = 9, □). Mice were killed 21-29 days posttransplantation, and colon explant tissue was assayed for levels of proinflammatory cytokines by multiplex. Data are derived from cumulative results from 2 experiments and are presented as the mean amount of cytokine divided by the weight of cultured colon tissue (± SEM). Statistics: * P  ≤ .05; ** P
    Collagenase D, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase d/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Roche collagenase a
    Illustration of the procedures for tongue epithelial sheet preparation. (A) Tongue on mandible dissected from the mice; (B) Tongue on mandible after the injection of <t>collagenase</t> A and dispase II; (C) Peeled epithelium sheet; (D) Flow of the major steps.
    Collagenase A, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase a/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase a - by Bioz Stars, 2021-04
    86/100 stars
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    CCL25 RNA is expressed in thymic endothelial cells and is also highly expressed in hyperreceptive thymi of IL-7R −/− and PSGL-1 −/− mice. (a) RNA levels of ICAM-1, VCAM-1, and CCL25 in the thymi of WT and RAG-1 −/− , C2GnT1 −/− , PSGL-1 −/− , IL-7R −/− , and Psel −/− mice, as determined by qrtPCR. RNA levels were normalized to VE-cadherin and HPRT expression and expressed relative to WT (=1, as indicated). (b) Relative expression of the indicated genes in FACS-sorted WT thymic endothelial cells (ENDO; CD45 − , CD31 + , CD144 + ), cortical epithelial cells (CEC; CD45 − , Ly51 hi , G8.8 + ), and medullary epithelial cells (MEC; CD45 − , Ly51 int , G8.8 + ) relative to expression in an unsorted sample. RNA levels were normalized to the reference genes HPRT and TbP. N.D., not detected. (c) Numbers of ENDO, CEC, and MEC in the thymi of the indicated mouse strains. For b and c, thymi were sequentially digested with collagenase and collagenase/dispase, and single-cell suspensions were analyzed by flow cytometry using reference beads to calculate absolute cell numbers. ENDO, CEC, and MEC were defined as in b. Data are representative of at least three independent experiments with at least five mice in a and c, and four mice in b (means ± SEM). Mice were sex matched and 35 ± 4 d old. C2, C2GnT1. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Thymic progenitor homing and lymphocyte homeostasis are linked via S1P-controlled expression of thymic P-selectin/CCL25

    doi: 10.1084/jem.20082502

    Figure Lengend Snippet: CCL25 RNA is expressed in thymic endothelial cells and is also highly expressed in hyperreceptive thymi of IL-7R −/− and PSGL-1 −/− mice. (a) RNA levels of ICAM-1, VCAM-1, and CCL25 in the thymi of WT and RAG-1 −/− , C2GnT1 −/− , PSGL-1 −/− , IL-7R −/− , and Psel −/− mice, as determined by qrtPCR. RNA levels were normalized to VE-cadherin and HPRT expression and expressed relative to WT (=1, as indicated). (b) Relative expression of the indicated genes in FACS-sorted WT thymic endothelial cells (ENDO; CD45 − , CD31 + , CD144 + ), cortical epithelial cells (CEC; CD45 − , Ly51 hi , G8.8 + ), and medullary epithelial cells (MEC; CD45 − , Ly51 int , G8.8 + ) relative to expression in an unsorted sample. RNA levels were normalized to the reference genes HPRT and TbP. N.D., not detected. (c) Numbers of ENDO, CEC, and MEC in the thymi of the indicated mouse strains. For b and c, thymi were sequentially digested with collagenase and collagenase/dispase, and single-cell suspensions were analyzed by flow cytometry using reference beads to calculate absolute cell numbers. ENDO, CEC, and MEC were defined as in b. Data are representative of at least three independent experiments with at least five mice in a and c, and four mice in b (means ± SEM). Mice were sex matched and 35 ± 4 d old. C2, C2GnT1. *, P

    Article Snippet: For sorting, individual thymi were successively digested in 0.2 mg/ml collagenase and 0.2 mg/ml collagenase/dispase (Roche) in RPMI 1640 containing 0.02 mg/ml DNase (Sigma-Aldrich) at 37°C, washed, and filtered through a 100-µm mesh (Thermo Fisher Scientific), as previously described ( ).

    Techniques: Mouse Assay, Expressing, FACS, Capillary Electrochromatography, Flow Cytometry, Cytometry

    Absence of donor APC-derived IL-23 significantly attenuates proinflammatory cytokine production in the colon . (A,B) Lethally irradiated (900 cGy) Balb/c mice were transplanted with B6 BM (10 × 10 6 ) and spleen cells (0.4-0.6 × 10 6 ) (■) or IL-23 −/−  BM (10 × 10 6 ) and spleen cells (□) adjusted to yield an equivalent number of mature T cells. Cohorts of mice (7-9/group) were killed at the indicated time points (days 4, 7, 14, 21, and 28), and segments of colon tissue from individual mice were cultured overnight in media. Colonic tissue supernatants were collected and analyzed for the amount of IL-23p19 (A) and IL-12p70 (B) by ELISA and multiplex, respectively. Data are presented as mean amount of cytokine divided by the weight of cultured colon tissue (± SEM) and are cumulative results from 2 experiments. (C) Groups of mice were transplanted as in panels A and B and killed 28 days posttransplantation. Colons (n = 4-5/group per experiment) were pooled and digested with collagenase D. Total number of isolated LPMCs that were CD11c +  CD11b +  or CD11b +  CD11c −  in Balb/c recipients of B6 BM and spleen cells (■) or IL-23 −/−  BM and spleen cells (□) is depicted. Data are derived from 3 independent experiments and are presented as the mean cell number (×1000) per mouse (± SEM) (D). Lethally irradiated Balb/c mice were transplanted with B6 BM plus spleen cells (n = 10, ■) or IL-23 −/−  BM and spleen cells adjusted to yield equivalent numbers of T cells (n = 9, □). Mice were killed 21-29 days posttransplantation, and colon explant tissue was assayed for levels of proinflammatory cytokines by multiplex. Data are derived from cumulative results from 2 experiments and are presented as the mean amount of cytokine divided by the weight of cultured colon tissue (± SEM). Statistics: * P  ≤ .05; ** P

    Journal: Blood

    Article Title: Interleukin-23 secretion by donor antigen-presenting cells is critical for organ-specific pathology in graft-versus-host disease

    doi: 10.1182/blood-2008-08-175448

    Figure Lengend Snippet: Absence of donor APC-derived IL-23 significantly attenuates proinflammatory cytokine production in the colon . (A,B) Lethally irradiated (900 cGy) Balb/c mice were transplanted with B6 BM (10 × 10 6 ) and spleen cells (0.4-0.6 × 10 6 ) (■) or IL-23 −/− BM (10 × 10 6 ) and spleen cells (□) adjusted to yield an equivalent number of mature T cells. Cohorts of mice (7-9/group) were killed at the indicated time points (days 4, 7, 14, 21, and 28), and segments of colon tissue from individual mice were cultured overnight in media. Colonic tissue supernatants were collected and analyzed for the amount of IL-23p19 (A) and IL-12p70 (B) by ELISA and multiplex, respectively. Data are presented as mean amount of cytokine divided by the weight of cultured colon tissue (± SEM) and are cumulative results from 2 experiments. (C) Groups of mice were transplanted as in panels A and B and killed 28 days posttransplantation. Colons (n = 4-5/group per experiment) were pooled and digested with collagenase D. Total number of isolated LPMCs that were CD11c + CD11b + or CD11b + CD11c − in Balb/c recipients of B6 BM and spleen cells (■) or IL-23 −/− BM and spleen cells (□) is depicted. Data are derived from 3 independent experiments and are presented as the mean cell number (×1000) per mouse (± SEM) (D). Lethally irradiated Balb/c mice were transplanted with B6 BM plus spleen cells (n = 10, ■) or IL-23 −/− BM and spleen cells adjusted to yield equivalent numbers of T cells (n = 9, □). Mice were killed 21-29 days posttransplantation, and colon explant tissue was assayed for levels of proinflammatory cytokines by multiplex. Data are derived from cumulative results from 2 experiments and are presented as the mean amount of cytokine divided by the weight of cultured colon tissue (± SEM). Statistics: * P ≤ .05; ** P

    Article Snippet: To isolate lamina propria mononuclear cells (LPMCs), pooled colons were incubated in Hank balanced salt solution (HBSS) buffer (Gibco-BRL Life Technologies) supplemented with 5% fetal bovine serum (FBS), 0.05 mM ethylenediaminetetraacetic acid (EDTA), 0.6 mM N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES) buffer, 100 U/mL penicillin/100 μg/mL streptomycin (Gibco-BRL Life Technologies), and 15 μg/mL dithiothreitol (Invitrogen, Carlsbad, CA) at 37°C for 30 minutes and subsequently digested in a solution of 0.2 mg/mL collagenase D (Roche Diagnostics, Indianapolis, IN) in RPMI medium for 1 hour at 37°C.

    Techniques: Derivative Assay, Irradiation, Mouse Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Isolation

    I.t. SLR14 delivery enhances tumor infiltration of cytotoxic T lymphocytes and myeloid cells. Subcutaneous YMR1.7 melanoma was established in C57BL/6J mice and i.t. treated with vehicle, SLR14, or no treatment. 3 d after the fifth treatment, tumors were harvested and digested with 0.5 mg/ml Collagenase D and 40 µg/ml DNase I. Single-cell suspensions were prepared for flow cytometry analysis. (A) Percentages (top) and quantities (bottom) of tumor-infiltrating CD45 + , CD11b + , CD8 + , CD4 + , FoxP3 + CD4 + , or NK1.1 + cells in each group. All T cells were CD44 + . The cell numbers were normalized based on the tumor weight. Error bars = SD. 1, no treatment; 2, vehicle; 3, SLR14. (B) The ratio of tumor-infiltrating CD8 + T cells/CD4 + T cells or CD8 + T cells/CD4 + FoxP3 + T reg cells in each group. Error bars = SD. (C) Subcutaneous YMR1.7 melanoma growth in RAG1 −/− mice treated with vehicle or SLR14. Treatment protocol was the same as described in Fig. 1 A . Left: Tumor growth curves (error bars = SD) for each group of mice. Right: Tumor growth curves of individual mice in each group. (D and E) IFNγ, TNFα, and GzmB productions of tumor-infiltrating CD8 + T lymphocytes after i.t. treatment (error bars = SD). Five mice per group. Unpaired t test was used for statistical analysis. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Intratumoral delivery of RIG-I agonist SLR14 induces robust antitumor responses

    doi: 10.1084/jem.20190801

    Figure Lengend Snippet: I.t. SLR14 delivery enhances tumor infiltration of cytotoxic T lymphocytes and myeloid cells. Subcutaneous YMR1.7 melanoma was established in C57BL/6J mice and i.t. treated with vehicle, SLR14, or no treatment. 3 d after the fifth treatment, tumors were harvested and digested with 0.5 mg/ml Collagenase D and 40 µg/ml DNase I. Single-cell suspensions were prepared for flow cytometry analysis. (A) Percentages (top) and quantities (bottom) of tumor-infiltrating CD45 + , CD11b + , CD8 + , CD4 + , FoxP3 + CD4 + , or NK1.1 + cells in each group. All T cells were CD44 + . The cell numbers were normalized based on the tumor weight. Error bars = SD. 1, no treatment; 2, vehicle; 3, SLR14. (B) The ratio of tumor-infiltrating CD8 + T cells/CD4 + T cells or CD8 + T cells/CD4 + FoxP3 + T reg cells in each group. Error bars = SD. (C) Subcutaneous YMR1.7 melanoma growth in RAG1 −/− mice treated with vehicle or SLR14. Treatment protocol was the same as described in Fig. 1 A . Left: Tumor growth curves (error bars = SD) for each group of mice. Right: Tumor growth curves of individual mice in each group. (D and E) IFNγ, TNFα, and GzmB productions of tumor-infiltrating CD8 + T lymphocytes after i.t. treatment (error bars = SD). Five mice per group. Unpaired t test was used for statistical analysis. *, P

    Article Snippet: Tumor digestion and flow cytometry analysis Tumors were harvested, cut into small pieces with surgical scissors and sharp blade, and then digested in HBSS containing 0.5 mg/ml Collagenase D (Roche) and 40 µg/ml DNase I (Roche) in a 37°C shaker for 20–30 min. Digestion was stopped by adding 0.5 mg/ml EDTA in HBSS, and single-cell suspensions were prepared for antibody staining.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry

    Illustration of the procedures for tongue epithelial sheet preparation. (A) Tongue on mandible dissected from the mice; (B) Tongue on mandible after the injection of collagenase A and dispase II; (C) Peeled epithelium sheet; (D) Flow of the major steps.

    Journal: Tissue Engineering. Part C, Methods

    Article Title: Taste Bud Labeling in Whole Tongue Epithelial Sheet in Adult Mice

    doi: 10.1089/ten.tec.2015.0377

    Figure Lengend Snippet: Illustration of the procedures for tongue epithelial sheet preparation. (A) Tongue on mandible dissected from the mice; (B) Tongue on mandible after the injection of collagenase A and dispase II; (C) Peeled epithelium sheet; (D) Flow of the major steps.

    Article Snippet: A mixture of collagenase A (1 mg/mL, Cat No. 10103578001; Roche Diagnostics) and dispase II (2.5 mg/mL; Cat No. 10374300; Roche Diagnostics) in 0.1 M phosphate-buffered saline (PBS, pH 7.4) (Cat No. CP4390-48; Denville Scientific, Inc.) was injected into the tongue from the posterior cutting end of the tongue using a 30G needle.

    Techniques: Mouse Assay, Injection, Flow Cytometry