collagenase type d  (Boehringer Mannheim)

 
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    Structured Review

    Boehringer Mannheim collagenase type d
    Collagenase Type D, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type d/product/Boehringer Mannheim
    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    collagenase type d - by Bioz Stars, 2021-01
    89/100 stars

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    Related Articles

    Modification:

    Article Title: Sequestration of G-Protein βγ Subunits by Different G-Protein α Subunits Blocks Voltage-Dependent Modulation of Ca2+Channels in Rat Sympathetic Neurons
    Article Snippet: .. The ganglia were desheathed, cut into small pieces, and incubated with 1 mg/ml collagenase type D, 0.35 mg/ml trypsin (Boehringer Mannheim Biochemicals, Indianapolis, IN), and 0.1 mg/ml DNase type I (Sigma) in 10 ml of modified Earle’s balance salt solution (EBSS, pH 7.4) in a 25 cm2 tissue culture flask. .. The EBSS was modified by adding 3.6 gm/l glucose and 10 m m HEPES.

    Incubation:

    Article Title: Sequestration of G-Protein βγ Subunits by Different G-Protein α Subunits Blocks Voltage-Dependent Modulation of Ca2+Channels in Rat Sympathetic Neurons
    Article Snippet: .. The ganglia were desheathed, cut into small pieces, and incubated with 1 mg/ml collagenase type D, 0.35 mg/ml trypsin (Boehringer Mannheim Biochemicals, Indianapolis, IN), and 0.1 mg/ml DNase type I (Sigma) in 10 ml of modified Earle’s balance salt solution (EBSS, pH 7.4) in a 25 cm2 tissue culture flask. .. The EBSS was modified by adding 3.6 gm/l glucose and 10 m m HEPES.

    Article Title: Coupling Specificity of NOP Opioid Receptors to Pertussis-Toxin-Sensitive G? Proteins in Adult Rat Stellate Ganglion Neurons Using Small Interference RNA
    Article Snippet: .. The neurons were incubated for 5 min in Earle's balanced salt solution (EBSS) containing 0.2 mg/ml collagenase type D (Boehringer Mannheim, Indianapolis, IN). ..

    Article Title: Multiple G-Protein βγ Combinations Produce Voltage-Dependent Inhibition of N-Type Calcium Channels in Rat Superior Cervical Ganglion Neurons
    Article Snippet: .. The ganglia were incubated with 0.6 mg/ml collagenase type D (Boehringer Mannheim, Indianapolis, IN), 0.4 mg/ml trypsin (TRL type; Worthington Biochemical Corp., Lakewood, NJ), and 0.1 mg/ml DNase Type I (Sigma, St. Louis, MO) for 60 min in a water bath shaker at 35°C. .. After incubation, the dispersed neurons were centrifuged twice for 6 min at 50 × g and then resuspended in Minimal Essential Medium (Mediatech, Inc., Herndon, VA) supplemented with 10% fetal calf serum (Atlanta Biologicals, Atlanta, GA), 1% glutamine, and 1% penicillin–streptomycin solution (both from Mediatech, Inc.).

    Article Title: Heterologous expression and coupling of G protein-gated inwardly rectifying K+ channels in adult rat sympathetic neurons
    Article Snippet: .. The ganglia were desheathed and multiple parallel slits (∼1 mm apart) were made perpendicular to the long axis prior to incubation with 0.7 mg ml−1 collagenase Type D, 0.3 mg ml−1 trypsin (both from Boehringer Mannheim) and 0.1 mg ml−1 DNase Type I (Sigma) for 1 h in a shaking water bath at 35°C. .. Following the incubation period, the cells were dissociated in a culture flask by vigorous shaking.

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    • collagenase  (Boehringer Mannheim)
    92
    Boehringer Mannheim collagenase
    Collagenase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase/product/Boehringer Mannheim
    Average 92 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    collagenase - by Bioz Stars, 2021-01
    92/100 stars
    		  Buy from Supplier

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