collagenase dispase  (Millipore)


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    Name:
    Collagenase Dispase
    Description:
    Dispase is a neutral rapid acting gentle protease that separates intact epidermis from dermis It also separates cultured intact epithelial cells from the substratum It cleaves the basement membrane zone and leaves the epithelial cells intact Collagenase is a protease which cleaves the triple helical protein called collagen There are three types of tissue collagenases and these belong to the matrix metalloproteinases MMP family Collagenase obtained from Clostridium histolyticum has a very strong activity as it digests collagen from both ends at temperatures as low as 4 10 C
    Catalog Number:
    colldisp-ro
    Price:
    None
    Applications:
    Collagenase/Dispase(R) is used for the preparation of cells from a wide variety of different tissues and organs.
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    Structured Review

    Millipore collagenase dispase
    Physiological hypoxia and cell derived matrix synergistically enhance proliferation of Patient Tumor Stromal-like cells. Resected tumors were enzymatically digested with <t>collagenase/dispase</t> and single cell suspensions were grown on four environmental conditions (2DN, 2DH, 3DN, 3DH). ( A ) Representative phase contrast images (from 5 random fields) of patient sample 927 cell growth 7 days post isolation in each condition. Scale bars are 100 μ m. ( B ) Phase contrast images of patient samples 956 and 959 obtained 7 and 6 days after isolation in 2DN and 3DH. ( C ) Phase contrast images of patient sample 955 tumor and adjacent normal lung cells 9 days post isolation. ( D ) Total cell numbers and days post isolation of 4 patient samples grown in 2D Normoxia and 3D Hypoxia at the time of first and third passaging. ( E ) (Upper) Doubling time of 3DH derived 927 cell line upon transfer to 4 environmental conditions. (Lower) Doubling time of patient derived cell lines maintained in 3DH or transferred to 2DN. Statistical analysis performed by two-tailed T-test, ***p
    Dispase is a neutral rapid acting gentle protease that separates intact epidermis from dermis It also separates cultured intact epithelial cells from the substratum It cleaves the basement membrane zone and leaves the epithelial cells intact Collagenase is a protease which cleaves the triple helical protein called collagen There are three types of tissue collagenases and these belong to the matrix metalloproteinases MMP family Collagenase obtained from Clostridium histolyticum has a very strong activity as it digests collagen from both ends at temperatures as low as 4 10 C
    https://www.bioz.com/result/collagenase dispase/product/Millipore
    Average 99 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    collagenase dispase - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Primary lung cancer samples cultured under microenvironment-mimetic conditions enrich for mesenchymal stem-like cells that promote metastasis"

    Article Title: Primary lung cancer samples cultured under microenvironment-mimetic conditions enrich for mesenchymal stem-like cells that promote metastasis

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-40519-4

    Physiological hypoxia and cell derived matrix synergistically enhance proliferation of Patient Tumor Stromal-like cells. Resected tumors were enzymatically digested with collagenase/dispase and single cell suspensions were grown on four environmental conditions (2DN, 2DH, 3DN, 3DH). ( A ) Representative phase contrast images (from 5 random fields) of patient sample 927 cell growth 7 days post isolation in each condition. Scale bars are 100 μ m. ( B ) Phase contrast images of patient samples 956 and 959 obtained 7 and 6 days after isolation in 2DN and 3DH. ( C ) Phase contrast images of patient sample 955 tumor and adjacent normal lung cells 9 days post isolation. ( D ) Total cell numbers and days post isolation of 4 patient samples grown in 2D Normoxia and 3D Hypoxia at the time of first and third passaging. ( E ) (Upper) Doubling time of 3DH derived 927 cell line upon transfer to 4 environmental conditions. (Lower) Doubling time of patient derived cell lines maintained in 3DH or transferred to 2DN. Statistical analysis performed by two-tailed T-test, ***p
    Figure Legend Snippet: Physiological hypoxia and cell derived matrix synergistically enhance proliferation of Patient Tumor Stromal-like cells. Resected tumors were enzymatically digested with collagenase/dispase and single cell suspensions were grown on four environmental conditions (2DN, 2DH, 3DN, 3DH). ( A ) Representative phase contrast images (from 5 random fields) of patient sample 927 cell growth 7 days post isolation in each condition. Scale bars are 100 μ m. ( B ) Phase contrast images of patient samples 956 and 959 obtained 7 and 6 days after isolation in 2DN and 3DH. ( C ) Phase contrast images of patient sample 955 tumor and adjacent normal lung cells 9 days post isolation. ( D ) Total cell numbers and days post isolation of 4 patient samples grown in 2D Normoxia and 3D Hypoxia at the time of first and third passaging. ( E ) (Upper) Doubling time of 3DH derived 927 cell line upon transfer to 4 environmental conditions. (Lower) Doubling time of patient derived cell lines maintained in 3DH or transferred to 2DN. Statistical analysis performed by two-tailed T-test, ***p

    Techniques Used: Derivative Assay, Isolation, Passaging, Two Tailed Test

    Related Articles

    Modification:

    Article Title: Primary lung cancer samples cultured under microenvironment-mimetic conditions enrich for mesenchymal stem-like cells that promote metastasis
    Article Snippet: .. The diced tissue was transferred to a vial containing collagenase/dispase (Sigma, St. Louis, MO) dissolved (1 mg/mL) in serum free Dulbecco’s modified Eagle’s medium-high glucose (DMEM) for enzymatic digestion. .. The vial was warmed to 37 °C and incubated with rocking for at least 1 h 15 m. The tissue suspension was agitated with vigorous pipetting to confirm tissue dissociation and either returned to 37 °C if incomplete or inactivated.

    Isolation:

    Article Title: Niaspan enhances vascular remodeling after stroke in type 1 diabetic rats enhances vascular remodeling after stroke in type 1 diabetic rats
    Article Snippet: .. Briefiy, the cortical brain tissue was isolated from WT and T1DM rats and digested in collagenase/dispase, and the microvessels separated by centrifugation in a Percoll (Sigma) gradient. ..

    Centrifugation:

    Article Title: Niaspan enhances vascular remodeling after stroke in type 1 diabetic rats enhances vascular remodeling after stroke in type 1 diabetic rats
    Article Snippet: .. Briefiy, the cortical brain tissue was isolated from WT and T1DM rats and digested in collagenase/dispase, and the microvessels separated by centrifugation in a Percoll (Sigma) gradient. ..

    Incubation:

    Article Title: Social stress induces neurovascular pathology promoting depression
    Article Snippet: .. The pellet was resuspended in DMEM supplemented with 1 mg/mL collagenase/dispase, 40 µg/mL DNase 1 and 0.147 µg/mL tosyllysine chloromethyl ketone (Sigma), and incubated at 37°C for 75 min with agitation to free endothelial cells from pericytes, perivascular macrophages and remains of the basement membrane. .. After centrifugation (4,000 g for 10 min at room temperature), the supernatant was discarded and the pellet was resuspended in RIPA lysis buffer.

    Article Title: Low Dose Leishmania major Promotes a Transient T Helper Cell Type 2 Response That Is Down-regulated by Interferon ?-producing CD8+ T Cells
    Article Snippet: .. In brief, the dorsal and ventral layers of the ear were separated with forceps and incubated for 30 min at 37°C in complete Grace's medium containing 1 mg/ml collagenase/dispase (Sigma-Aldrich). .. Thereafter, the ears were minced and homogenized in 2 ml Teflon-coated tissue grinder and the homogenate was serially diluted (1:2) in 96-well plates and incubated at 26°C.

    Staining:

    Article Title: The Circuitry of Olfactory Projection Neurons in the Brain of the Honeybee, Apis mellifera
    Article Snippet: .. For whole mount antibody staining a treatment with 0.1% (w/v) collagenase-dispase, 0.1% (w/v) hyaluronidase (Sigma-Aldrich, München, Germany) in PBS for 30 min at 36°C. .. In addition, the brains were incubated for 20 min in sodium borohydride 1% (w/v) in PBS.

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  • 99
    Millipore collagenase dispase
    Collagenase Dispase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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