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Boehringer Mannheim collagenase dispase
Collagenase Dispase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase dispase/product/Boehringer Mannheim
Average 92 stars, based on 29 article reviews
Price from $9.99 to $1999.99
collagenase dispase - by Bioz Stars, 2021-01
92/100 stars

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Modification:

Article Title: Methylprednisolone blocks interleukin 1 beta induced calcitonin gene related peptide release in trigeminal ganglia cells
Article Snippet: .. The cells were incubated for 90 min at 37 °C in 10 ml dissociation medium (modified eagles medium; Biochrom, Berlin, Germany; with 10 % bovine serum, 10 mM HEPES, 44 mM glucose, 100 U penicillin + streptomycin, 2 mM glutamine, 100 IE insulin/l) containing collagenase/dispase (final concentration 100 μg/ml) (Boehringer Mannheim, Germany), rinsed twice with phosphate buffered saline (PBS) 0.1 M and again incubated with trypsin/EDTA (0.05 %/0.02 % w/v in PBS) for 30 min for dissociation. .. Subsequently, cells were rinsed twice with PBS and once with dissociation medium, dissociated by Pasteur pipette and pelleted by centrifugation at 2100 x g for 2 min at 21 °C.

Concentration Assay:

Article Title: Methylprednisolone blocks interleukin 1 beta induced calcitonin gene related peptide release in trigeminal ganglia cells
Article Snippet: .. The cells were incubated for 90 min at 37 °C in 10 ml dissociation medium (modified eagles medium; Biochrom, Berlin, Germany; with 10 % bovine serum, 10 mM HEPES, 44 mM glucose, 100 U penicillin + streptomycin, 2 mM glutamine, 100 IE insulin/l) containing collagenase/dispase (final concentration 100 μg/ml) (Boehringer Mannheim, Germany), rinsed twice with phosphate buffered saline (PBS) 0.1 M and again incubated with trypsin/EDTA (0.05 %/0.02 % w/v in PBS) for 30 min for dissociation. .. Subsequently, cells were rinsed twice with PBS and once with dissociation medium, dissociated by Pasteur pipette and pelleted by centrifugation at 2100 x g for 2 min at 21 °C.

Article Title: Requirement for splenic CD4+ T cells in the immune privilege of the anterior chamber of the eye
Article Snippet: .. To analyse gene expressions of cytokine in the eye, tumour-bearing eyes pooled from each group of five mice were enucleated on day 7 after the inoculation, minced with scissors, and treated with collagenase/dispase (Boehringer, Mannheim, Germany) at a final concentration of 1 mg/ml for 90 min at 37°C. .. For recovering lymphocytes from the monodispersed cells, mixtures of lymphocytes and tumour cells were first prepared by Ficoll–Isopaque density (1090) gradient centrifugation, and then both CD4+ and CD8+ T cells were selectively separated using immune beads from the mixture.

Incubation:

Article Title: Methylprednisolone blocks interleukin 1 beta induced calcitonin gene related peptide release in trigeminal ganglia cells
Article Snippet: .. The cells were incubated for 90 min at 37 °C in 10 ml dissociation medium (modified eagles medium; Biochrom, Berlin, Germany; with 10 % bovine serum, 10 mM HEPES, 44 mM glucose, 100 U penicillin + streptomycin, 2 mM glutamine, 100 IE insulin/l) containing collagenase/dispase (final concentration 100 μg/ml) (Boehringer Mannheim, Germany), rinsed twice with phosphate buffered saline (PBS) 0.1 M and again incubated with trypsin/EDTA (0.05 %/0.02 % w/v in PBS) for 30 min for dissociation. .. Subsequently, cells were rinsed twice with PBS and once with dissociation medium, dissociated by Pasteur pipette and pelleted by centrifugation at 2100 x g for 2 min at 21 °C.

Article Title: Vaccination with Bordetella pertussis-Pulsed Autologous or Heterologous Dendritic Cells Induces a Mucosal Antibody Response In Vivo and Protects against Infection
Article Snippet: .. For the enumeration of ASC in the lungs, individual lungs were cut into small pieces, suspended in an enzyme solution consisting of Hanks balanced salt solution with 1 mg of collagenase-Dispase (Boehringer Mannheim)/ml and 0.25 mg of DNAse 1 type IV (Sigma)/ml, incubated at 37°C for 30 min, filtered through a 150-μm-pore-size nylon mesh, and analyzed by enzyme-linked immunospot assay ( ) for numbers of IgG and IgA ASC specific for B. pertussis , on nitrocellulose plates coated with bacterial sonicate (prepared as described previously; see reference ). ..

Article Title: Mice Lacking Specific Nicotinic Acetylcholine Receptor Subunits Exhibit Dramatically Altered Spontaneous Activity Patterns and Reveal a Limited Role for Retinal Waves in Forming ON and OFF Circuits in the Inner Retina
Article Snippet: .. To facilitate peptide penetration into retinal tissue, the retinas were incubated in 2 mg/ml collagenase/dispase (Boehringer Mannheim, Indianapolis, IN) for 10 min at 30°C. .. Although this treatment was found to alter the spatiotemporal properties of waves in normal mice, primarily by restricting the size of propagating waves ( n = 3), this treatment was necessary to allow conotoxins to penetrate into the tissue (Fig. C ).

other:

Article Title: Noninvasive neuroelectronic interfacing with synaptically connected snail neurons immobilized on a semiconductor chip
Article Snippet: After removing the outer sheath, the brains were treated with 1.33 mg/ml collagenase/dispase (Boehringer Mannheim) and 0.67 mg/ml trypsin (Sigma, T8253) for 30 min in defined medium, washed several times, treated with 0.67 mg/ml trypsin inhibitor (Sigma, T9003) in defined medium for 15 min, and washed again.

Article Title: 12/15-Lipoxygenase-Dependent Myeloid Production of Interleukin-12 Is Essential for Resistance to Chronic Toxoplasmosis ▿
Article Snippet: Following excision, brains were minced with scissors and then digested for 1 h at 37°C with 300 μg of collagenase/dispase (Boehringer Mannhein, Indianapolis, IN) and 600 μg of DNase I (Boehringer Mannheim) per ml in complete RPMI medium.

Mouse Assay:

Article Title: Requirement for splenic CD4+ T cells in the immune privilege of the anterior chamber of the eye
Article Snippet: .. To analyse gene expressions of cytokine in the eye, tumour-bearing eyes pooled from each group of five mice were enucleated on day 7 after the inoculation, minced with scissors, and treated with collagenase/dispase (Boehringer, Mannheim, Germany) at a final concentration of 1 mg/ml for 90 min at 37°C. .. For recovering lymphocytes from the monodispersed cells, mixtures of lymphocytes and tumour cells were first prepared by Ficoll–Isopaque density (1090) gradient centrifugation, and then both CD4+ and CD8+ T cells were selectively separated using immune beads from the mixture.

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    Boehringer Mannheim dispase
    Dispase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dispase/product/Boehringer Mannheim
    Average 92 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    dispase - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim collagenase dispase
    Collagenase Dispase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase dispase/product/Boehringer Mannheim
    Average 92 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    collagenase dispase - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

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