collagenase dispase solution  (Millipore)


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    Name:
    Collagenase D
    Description:
    Collagenase D is prepared from Clostridium histolyticum cultures by filtration ammonium sulfate precipitation dialysis and lyophilization
    Catalog Number:
    colld-ro
    Price:
    None
    Applications:
    Collagenase from C. histolyticum is widely used for the disaggregation of many types of tissues (e.g., lung, heart, muscle, bone, adipose tissue, liver, kidney, cartilage, mammary gland, placentae, blood vessels, brain, tumors) and for the preparation of single cell suspensions for the establishment of primary cell culture systems. Collagenase D is recommended when functionality and integrity of cell-surface proteins are important.Clostridium collagenase from Roche has been used to prepare cells from many types of tissue, such as hepatocytes, adipocytes, pancreatic islets, epithelial cells, muscle cells, endothelial cells, etc. However, suitability of each lot of the enzyme for disruption of a particular tissue should be determined empirically.
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    Structured Review

    Millipore collagenase dispase solution
    Collagenase D
    Collagenase D is prepared from Clostridium histolyticum cultures by filtration ammonium sulfate precipitation dialysis and lyophilization
    https://www.bioz.com/result/collagenase dispase solution/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    collagenase dispase solution - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Dysregulated Cytokine Expression by CD4+ T cells from Post-Septic Mice Modulates both Th1 and Th2-Mediated Granulomatous Lung Inflammation
    Article Snippet: .. Flow cytometry Collagenase-digested (Sigma-Aldrich, St. Louis, MO) lung lobes and undigested lymph nodes from animals were processed into single cell suspensions by processing tissues through sterile 40-mm filters, and ammonium chloride lysis buffer was used to eliminate erythrocytes. .. Cells were stained with the following fluorescent antibodies in flow cytometry buffer (phosphate buffered saline, 1% w/v bovine serum albumin, 0.05% w/v sodium azide): Purified αCD16/32 (Fc Block) (2.4G2, BD Biosciences, San Jose, CA), FITC-αCD8a (53–6.7, BD Biosciences), FITC-αCD3ε (145-2C11, BD Biosciences), PeCy7-αCD45 (Ly5, eBioscience, San Diego, CA), PeCy7-αCD3ε (17A2, BioLegend, San Diego, CA), Pacific Blue- (RM4-5, BioLegend) or Pacific Orange (RM4-5, Invitrogen, Carlsbad, CA) -αCD4, and Pacific Blue-αCD45 (30-F11, BioLegend).

    Isolation:

    Article Title: Analysis of Tks4 Knockout Mice Suggests a Role for Tks4 in Adipose Tissue Homeostasis in the Context of Beigeing
    Article Snippet: .. Cell Isolation, Analysis, and Culture 10–14-week-old Tks4−/− and WT mice were euthanized, and sWAT, gWAT, and iBAT fat pads were isolated, minced, and incubated in digestive medium containing 0.1% (m /v ) (for sWAT and gWAT) or 0.2% (m /v ) (for iBAT) type IV collagenase in Dulbecco’s phosphate-buffered saline (all from Sigma-Aldrich) for 1 h at 37 °C on a shaker. .. The tissue digestion was stopped by adding an equal volume of fetal bovine serum (FBS), and the resulting cell suspensions were centrifuged twice at 300× g for 5 min in Hank’s balanced salt solution (HBSS) (all from Invitrogen, Carlsbad, CA, USA) to isolate the stromal vascular fraction (SVF).

    Article Title: Cooperative function of Pdx1 and Oc1 in multipotent pancreatic progenitors impacts postnatal islet maturation and adaptability
    Article Snippet: .. Isolation of islets from P14 pancreas required collagenase digestion of whole dissected pancreas at 37°C and hand-picking of islets away from exocrine tissue following a Histopaque-1077 (Sigma-Aldrich) gradient. ..

    Cytometry:

    Article Title: Dysregulated Cytokine Expression by CD4+ T cells from Post-Septic Mice Modulates both Th1 and Th2-Mediated Granulomatous Lung Inflammation
    Article Snippet: .. Flow cytometry Collagenase-digested (Sigma-Aldrich, St. Louis, MO) lung lobes and undigested lymph nodes from animals were processed into single cell suspensions by processing tissues through sterile 40-mm filters, and ammonium chloride lysis buffer was used to eliminate erythrocytes. .. Cells were stained with the following fluorescent antibodies in flow cytometry buffer (phosphate buffered saline, 1% w/v bovine serum albumin, 0.05% w/v sodium azide): Purified αCD16/32 (Fc Block) (2.4G2, BD Biosciences, San Jose, CA), FITC-αCD8a (53–6.7, BD Biosciences), FITC-αCD3ε (145-2C11, BD Biosciences), PeCy7-αCD45 (Ly5, eBioscience, San Diego, CA), PeCy7-αCD3ε (17A2, BioLegend, San Diego, CA), Pacific Blue- (RM4-5, BioLegend) or Pacific Orange (RM4-5, Invitrogen, Carlsbad, CA) -αCD4, and Pacific Blue-αCD45 (30-F11, BioLegend).

    Infection:

    Article Title: Resolution of a chronic viral infection after interleukin-10 receptor blockade
    Article Snippet: .. Splenocytes from mice infected 7 d earlier with LCMV Armstrong or LCMV clone 13 with or without anti–IL-10R antibody treatment were incubated with HBSS medium containing 0.5 mg/ml collagenase D (Sigma-Aldrich) at 37°C, 5% CO2 for 30 min. 0.01 M EDTA was added to disrupt T cell–DC complexes. .. Next, cells were depleted of CD3-expressing cells (Dynal CD3-beads; Dynal), incubated with CD11c microbeads (Miltenyi Biotec), and positively selected using MACS columns.

    Mouse Assay:

    Article Title: Resolution of a chronic viral infection after interleukin-10 receptor blockade
    Article Snippet: .. Splenocytes from mice infected 7 d earlier with LCMV Armstrong or LCMV clone 13 with or without anti–IL-10R antibody treatment were incubated with HBSS medium containing 0.5 mg/ml collagenase D (Sigma-Aldrich) at 37°C, 5% CO2 for 30 min. 0.01 M EDTA was added to disrupt T cell–DC complexes. .. Next, cells were depleted of CD3-expressing cells (Dynal CD3-beads; Dynal), incubated with CD11c microbeads (Miltenyi Biotec), and positively selected using MACS columns.

    Article Title: Analysis of Tks4 Knockout Mice Suggests a Role for Tks4 in Adipose Tissue Homeostasis in the Context of Beigeing
    Article Snippet: .. Cell Isolation, Analysis, and Culture 10–14-week-old Tks4−/− and WT mice were euthanized, and sWAT, gWAT, and iBAT fat pads were isolated, minced, and incubated in digestive medium containing 0.1% (m /v ) (for sWAT and gWAT) or 0.2% (m /v ) (for iBAT) type IV collagenase in Dulbecco’s phosphate-buffered saline (all from Sigma-Aldrich) for 1 h at 37 °C on a shaker. .. The tissue digestion was stopped by adding an equal volume of fetal bovine serum (FBS), and the resulting cell suspensions were centrifuged twice at 300× g for 5 min in Hank’s balanced salt solution (HBSS) (all from Invitrogen, Carlsbad, CA, USA) to isolate the stromal vascular fraction (SVF).

    Concentration Assay:

    Article Title: Characterisation of gut, lung, and upper airways microbiota in patients with non-small cell lung carcinoma
    Article Snippet: .. The homogenate obtained will be treated with collagenase D (Sigma-Aldrich) (2 mg/mL final concentration) at 37°C for 15 min, followed by 10 min at 2000 × g at room temperature (RT). .. The pellet will be resuspended in 2–5 mL (depending on the initial sample weight) of mammalian cell lysis buffer (MCLB),[ ] and repeatedly vortexed for 5 min at RT.

    Incubation:

    Article Title: Resolution of a chronic viral infection after interleukin-10 receptor blockade
    Article Snippet: .. Splenocytes from mice infected 7 d earlier with LCMV Armstrong or LCMV clone 13 with or without anti–IL-10R antibody treatment were incubated with HBSS medium containing 0.5 mg/ml collagenase D (Sigma-Aldrich) at 37°C, 5% CO2 for 30 min. 0.01 M EDTA was added to disrupt T cell–DC complexes. .. Next, cells were depleted of CD3-expressing cells (Dynal CD3-beads; Dynal), incubated with CD11c microbeads (Miltenyi Biotec), and positively selected using MACS columns.

    Article Title: Analysis of Tks4 Knockout Mice Suggests a Role for Tks4 in Adipose Tissue Homeostasis in the Context of Beigeing
    Article Snippet: .. Cell Isolation, Analysis, and Culture 10–14-week-old Tks4−/− and WT mice were euthanized, and sWAT, gWAT, and iBAT fat pads were isolated, minced, and incubated in digestive medium containing 0.1% (m /v ) (for sWAT and gWAT) or 0.2% (m /v ) (for iBAT) type IV collagenase in Dulbecco’s phosphate-buffered saline (all from Sigma-Aldrich) for 1 h at 37 °C on a shaker. .. The tissue digestion was stopped by adding an equal volume of fetal bovine serum (FBS), and the resulting cell suspensions were centrifuged twice at 300× g for 5 min in Hank’s balanced salt solution (HBSS) (all from Invitrogen, Carlsbad, CA, USA) to isolate the stromal vascular fraction (SVF).

    Article Title: Polarization of macrophages in the tumor microenvironment is influenced by EGFR signaling within colon cancer cells
    Article Snippet: .. Briefly, colon tissues were cut into small pieces (1-2 mm) and incubated in 10 mL PRMI 1640 medium with 10 mM HEPES and 5% fetal bovine serum containing 10 mg (1 mg/mL) collagenase D (Sigma-Aldrich), 10 mg (1 mg/mL) dispase II (Roche, Germany), and 100 μL 10 mg/ml DNase I (100 μg/mL) (Sigma-Aldrich) for 30-45 min in a shaking incubator at 37°C. .. For Ana-1 and BMDM cells, single-cell suspensions from mouse colon tissues were incubated with APC-anti-mouse CD11b and FITC-anti-mouse F4/80 (Affymetrix eBioscience, USA), or APC-anti-mouse F4/80 and FITC-anti-mouse CD206 (MMR) (Biolegend, San Diego, CA) antibodies.

    Lysis:

    Article Title: Dysregulated Cytokine Expression by CD4+ T cells from Post-Septic Mice Modulates both Th1 and Th2-Mediated Granulomatous Lung Inflammation
    Article Snippet: .. Flow cytometry Collagenase-digested (Sigma-Aldrich, St. Louis, MO) lung lobes and undigested lymph nodes from animals were processed into single cell suspensions by processing tissues through sterile 40-mm filters, and ammonium chloride lysis buffer was used to eliminate erythrocytes. .. Cells were stained with the following fluorescent antibodies in flow cytometry buffer (phosphate buffered saline, 1% w/v bovine serum albumin, 0.05% w/v sodium azide): Purified αCD16/32 (Fc Block) (2.4G2, BD Biosciences, San Jose, CA), FITC-αCD8a (53–6.7, BD Biosciences), FITC-αCD3ε (145-2C11, BD Biosciences), PeCy7-αCD45 (Ly5, eBioscience, San Diego, CA), PeCy7-αCD3ε (17A2, BioLegend, San Diego, CA), Pacific Blue- (RM4-5, BioLegend) or Pacific Orange (RM4-5, Invitrogen, Carlsbad, CA) -αCD4, and Pacific Blue-αCD45 (30-F11, BioLegend).

    Cell Isolation:

    Article Title: Analysis of Tks4 Knockout Mice Suggests a Role for Tks4 in Adipose Tissue Homeostasis in the Context of Beigeing
    Article Snippet: .. Cell Isolation, Analysis, and Culture 10–14-week-old Tks4−/− and WT mice were euthanized, and sWAT, gWAT, and iBAT fat pads were isolated, minced, and incubated in digestive medium containing 0.1% (m /v ) (for sWAT and gWAT) or 0.2% (m /v ) (for iBAT) type IV collagenase in Dulbecco’s phosphate-buffered saline (all from Sigma-Aldrich) for 1 h at 37 °C on a shaker. .. The tissue digestion was stopped by adding an equal volume of fetal bovine serum (FBS), and the resulting cell suspensions were centrifuged twice at 300× g for 5 min in Hank’s balanced salt solution (HBSS) (all from Invitrogen, Carlsbad, CA, USA) to isolate the stromal vascular fraction (SVF).

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  • 99
    Millipore dispase ii mix
    Dispase Ii Mix, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dispase ii mix/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dispase ii mix - by Bioz Stars, 2021-01
    99/100 stars
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