collagenase d  (Worthington Biochemical)


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  • 97
    Name:
    Collagenase Type 4
    Description:
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    Catalog Number:
    ls004186
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase d
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase d/product/Worthington Biochemical
    Average 97 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    collagenase d - by Bioz Stars, 2021-01
    97/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Myocardial infarction stabilization by cell‐based expression of controlled Vascular Endothelial Growth Factor levels
    Article Snippet: .. Tissue was minced and digested with 0.15% collagenase (Worthington Biochemical Corporation, Lakewood, NJ) in phosphate‐buffered saline (PBS) at 37°C under continuous shaking for 60 min. After centrifugation at 1500 rpm for 10 min, the lipid‐rich layer was discarded and the cellular pellet was washed once with PBS. .. Released cells were strained through a 100‐μm nylon‐mesh followed by a 70‐μm nylon‐mesh to remove fibrous debris, plated at a density of 105 cells/cm2 and cultured in high‐glucose Dulbecco's modified Eagle's medium with 10% foetal bovine serum, as described .

    Homogenization:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Cell Culture:

    Article Title: Role of MAML1 in targeted therapy against the esophageal cancer stem cells
    Article Snippet: .. The sample was minced into 1 mm pieces and transferred to D-Hank’s buffer containing collagenase type I (0.25 mg/ml; Worthington Biomedical Corporation) and incubated at 37 °C for 3 h. Single cells were cultured in a specific complete medium comprising DMEM/F12 supplemented with penicillin/streptomycin (10 units/ml), insulin (25 µg/ml), epidermal growth factor (EGF, 20 ng/ml), and basic fibroblast growth factor (bFGF, 10 ng/ml) in a humidified atmosphere containing 5% CO2 at 37 °C. .. For tumor sphere formation, cancer cells were cultured for 3 weeks in low attachment plates.

    Purification:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Concentration Assay:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Incubation:

    Article Title: Role of MAML1 in targeted therapy against the esophageal cancer stem cells
    Article Snippet: .. The sample was minced into 1 mm pieces and transferred to D-Hank’s buffer containing collagenase type I (0.25 mg/ml; Worthington Biomedical Corporation) and incubated at 37 °C for 3 h. Single cells were cultured in a specific complete medium comprising DMEM/F12 supplemented with penicillin/streptomycin (10 units/ml), insulin (25 µg/ml), epidermal growth factor (EGF, 20 ng/ml), and basic fibroblast growth factor (bFGF, 10 ng/ml) in a humidified atmosphere containing 5% CO2 at 37 °C. .. For tumor sphere formation, cancer cells were cultured for 3 weeks in low attachment plates.

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    Worthington Biochemical d hank s buffer
    D Hank S Buffer, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d hank s buffer/product/Worthington Biochemical
    Average 99 stars, based on 2 article reviews
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    d hank s buffer - by Bioz Stars, 2021-01
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    97
    Worthington Biochemical collagenase d
    Collagenase D, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 97/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase d/product/Worthington Biochemical
    Average 97 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    collagenase d - by Bioz Stars, 2021-01
    97/100 stars
      Buy from Supplier

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