collagenase d  (Thermo Fisher)


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    Name:
    Collagenase Type IV powder
    Description:
    Collagenase is a protease that cleaves the bond between a neutral amino acid X and glycine in the sequence Pro X Glyc Pro which is found with high frequency in collagen Collagenase is unique among proteases in its ability to degrade the triple helical native collagen fibrils commonly found in connective tissues such as skin tendon blood vessels and bone Collagenase disaggregation is suitable for the culture of human tumors mouse kidney human adult and fetal brain and many other tissues including epithelium Collagenase is relatively gentle dissociates well at physiological temperature and pH and requires no mechanical agitation or special equipment Gibco Collagenase Type IV is isolated from Clostridium histolyticum and packaged as a lyophilized non sterile powder for research use in cell or tissue dissociation and organ perfusions Gibco Collagenase Type IV activity is guaranteed to be greater than 160 units mg Compared to other collagenase preparations Gibco Collagenase Type IV has low tryptic activity and is well suited for the digestion of islet cells from the pancreas
    Catalog Number:
    17104019
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher collagenase d
    Collagenase is a protease that cleaves the bond between a neutral amino acid X and glycine in the sequence Pro X Glyc Pro which is found with high frequency in collagen Collagenase is unique among proteases in its ability to degrade the triple helical native collagen fibrils commonly found in connective tissues such as skin tendon blood vessels and bone Collagenase disaggregation is suitable for the culture of human tumors mouse kidney human adult and fetal brain and many other tissues including epithelium Collagenase is relatively gentle dissociates well at physiological temperature and pH and requires no mechanical agitation or special equipment Gibco Collagenase Type IV is isolated from Clostridium histolyticum and packaged as a lyophilized non sterile powder for research use in cell or tissue dissociation and organ perfusions Gibco Collagenase Type IV activity is guaranteed to be greater than 160 units mg Compared to other collagenase preparations Gibco Collagenase Type IV has low tryptic activity and is well suited for the digestion of islet cells from the pancreas
    https://www.bioz.com/result/collagenase d/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase d - by Bioz Stars, 2021-04
    99/100 stars

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    Incubation:

    Article Title: Differentiation of Human Embryonic Stem Cells to Regional Specific Neural Precursors in Chemically Defined Medium Conditions
    Article Snippet: .. Human embryonic stem cells were passaged by incubation in 1 mg/ml collagenase IV (animal-free, Invitrogen) for 5–8 minutes at 37°C or mechanically dissociated and then removed to freshly prepared human foreskin fibroblast layer. .. Neural Differentiation Two to three pieces of domed hESC colonies were transferred to human matrix coated plates composed of 10 µg/cm2 human collagen IV (Sigma), 0.2 µg/cm2 human vitronectin (Sigma) and 5 µg/cm2 human fibronectin (Sigma) in modified TeSR1 medium .

    Article Title: Autophagy regulates testosterone synthesis by facilitating cholesterol uptake in Leydig cells
    Article Snippet: In brief, the 3-mo-old mice were euthanized using cervical dislocation, and the testes were removed and washed three times with PBS. .. Subsequently, the testes were decapsulated and incubated with 1 mg/ml collagenase IV in DMEM/F12 containing 5% FBS (10270; Gibco) in a shaking water bath (120 cycles/min) at 37°C for 15 min. After incubation, cold DMEM/F12 was added to stop the action of collagenase IV. .. Seminiferous tubules were separated from the interstitial cells by gravity sedimentation.

    Article Title: Characterization of Synthetic Tf2 as a NaV1.3 Selective Pharmacological Probe
    Article Snippet: Ca2+ Imaging of Isolated Dorsal Root Ganglion Neurons Primary cultures of mouse DRG neurons were prepared as previously described [ , ]. .. Briefly, DRG ganglia, removed from all spinal levels of 4-week-old male C57BL/6 mice, were incubated in DMEM (Gibco, Waltham, MD, USA) with 1 mg mL−1 Collagenase IV (Gibco, Life Technologies, NY, USA) for 90 min at 37 °C, then triturated with fire-polished Pasteur pipettes and plated in 96-well plates coated with PDL (Corning, ME, USA). .. After 1 h, wells were flooded with DMEM supplemented with 10% FBS and pencillin/streptomycin (Gibco, Waltham, MD, USA) and maintained for 16–24 h before assay.

    MTT Assay:

    Article Title: Procyanidins Inhibit Tumor Angiogenesis by Crosslinking Extracellular Matrix
    Article Snippet: .. MMP-2 (Cat. No. 17104-019; 265.00 units/mg) and 3-(4,5-dimethylthiazol-2 -yl)-2,5-di-phenyl tetrazolium bromide (MTT) were bought from Invitrogen. .. Glutaraldehyde, triton χ-100, sodium deoxycholate, ethylenediaminetetra-acetic acid (EDTA), ribonuclease A and deoxyribonuclease were purchased from Sigma Aldrich.

    Isolation:

    Article Title: KCa1.1 and Kv1.3 channels regulate the interactions between fibroblast-like synoviocytes and T lymphocytes during rheumatoid arthritis
    Article Snippet: FLS from patients with RA, as defined by criteria of the American College of Rheumatology [ ], were isolated as described previously [ ]. .. FLS from rats with CIA, induced with disease as described below, were isolated 14 days after the rats developed signs of disease, as described previously [ ] by isolating the synovial paw joints, incubating them with Gibco type IV collagenase (Life Technologies, Carlsbad, CA, USA) for 1 h at 37 °C, and culturing adherent cells in DMEM supplemented with 2 mg/ml L-glutamine, 0.1 μg/ml streptomycin, 10 U/ml penicillin, and 10% FBS. ..

    Mouse Assay:

    Article Title: Characterization of Synthetic Tf2 as a NaV1.3 Selective Pharmacological Probe
    Article Snippet: Ca2+ Imaging of Isolated Dorsal Root Ganglion Neurons Primary cultures of mouse DRG neurons were prepared as previously described [ , ]. .. Briefly, DRG ganglia, removed from all spinal levels of 4-week-old male C57BL/6 mice, were incubated in DMEM (Gibco, Waltham, MD, USA) with 1 mg mL−1 Collagenase IV (Gibco, Life Technologies, NY, USA) for 90 min at 37 °C, then triturated with fire-polished Pasteur pipettes and plated in 96-well plates coated with PDL (Corning, ME, USA). .. After 1 h, wells were flooded with DMEM supplemented with 10% FBS and pencillin/streptomycin (Gibco, Waltham, MD, USA) and maintained for 16–24 h before assay.

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  • 99
    Thermo Fisher collagenase d
    Collagenase D, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase d/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase d - by Bioz Stars, 2021-04
    99/100 stars
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