collagenase d (Boehringer Mannheim)

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Boehringer Mannheim collagenase d
Collagenase D, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase d/product/Boehringer Mannheim
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

collagenase d - by Bioz Stars, 2021-04

86/100 stars

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Incubation:

Article Title: Swift Entry of Myelin-Specific T Lymphocytes into the Central Nervous System in Spontaneous Autoimmune Encephalomyelitis
Article Snippet: .. Deep cervical, superficial cervical, inguinal lymph nodes, and the CNS (spinal cord, brain stem, and cerebellum) were dissected and incubated in 10 mg/ml collagenase D (Boehringer Mannheim) at 37°C for 45 min. ..

Article Title: Increased Antigen Specific T Cell Numbers in the Absence of Altered Migration or Division Rates as a Result of Mucosal Cholera Toxin Administration
Article Snippet: In brief, PP were removed from the intestine and incubated with HBSS/0.25 M EDTA to remove epithelial cells. .. Treated PP were then minced with a scalpel blade and further incubated with 200 µg/ml collagenase D (Boehringer Mannheim, Mannheim, Germany) and 40 µg/ml DNase I (Boehringer Mannheim, Mannheim, Germany) for 45 minutes at 37°C with gentle agitation. ..

Article Title: A Quantitative Morphometric Analysis of Rat Spinal Cord Remyelination Following Transplantation of Allogenic Schwann Cells
Article Snippet: Schwann cells from P7 neonatal rat sciatic nerves were dissociated by mechanical trituration following a 30-minute incubation with 2.5 mg/ml trypsin (Boehringer Manheim, Mannheim, Germany) in calcium-free complete saline solution (CSS) as described in . .. Adult sciatic nerves were desheathed, minced, incubated for 45 minutes in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL, Grand Island, NY) with 10 mg/ml each collagenase A + collagenase D (Boehringer Mannheim), pelleted, incubated for an additional 15 minutes in CSS with 2.5 mg/ml trypsin, and triturated though a fire-polished siliconized pasture pipette. .. Schwann cells were washed twice with DMEM + 10% fetal bovine serum before plating, and an additional 3 times in DMEM only before transplantation.

Modification:

Article Title: Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging
Article Snippet: Hearts are rapidly removed from anesthetized mice via cardiectomy and immediately mounted on a temperature controlled (37°C) perfusion system. .. After perfusion with modified Tyrode solution (Ca2+ free) for 2 min, the heart is digested for 15-20 min with 0.9 mg/ml collagenase D (Boehringer Mannheim Biochemicals) in modified Tyrode solution. .. The modified Tyrode solution (pH 7.4) contained the following (in mM): NaCl 135, KCl 4.0, MgCl2 1.0, HEPES 10, NaH2 PO4 0.33, glucose 10, butanedione 10, and the solution was equilibrated with 5% CO2 -95% O2 .

Transferring:

other:

Isolation:

Article Title: Structural Domains of the CB1 Cannabinoid Receptor That Contribute to Constitutive Activity and G-Protein Sequestration
Article Snippet: .. Isolated superior cervical ganglia were treated with 0.3 mg/ml trypsin, 0.45 mg/ml collagenase D (Boehringer Mannheim, Indianapolis, IN), and 0.1 mg/ml DNase in Earle's balanced salt solution for 1 hr at 35°C in a shaking water bath. .. Dissociated neurons were plated onto poly- l -lysine-coated 35 mm culture dishes in MEM (Life Technologies, Gaithersburg, MD) with 10% fetal calf serum, 1% glutamine, and 1% penicillin–streptomycin.

Injection:

Article Title: Depletion of host Langerhans cells before transplantation of donor alloreactive T cells prevents skin graft-versus-host disease
Article Snippet: .. We harvested spleen, liver and lymph nodes and injected them with collagenase D (1 mg/ml; Boehringer Mannheim) for 45 min (spleen and lymph nodes) or 90 min (liver) at 37 °C. .. We resuspended digested organs in medium and layered the cells above a Nycoprep solution (Nycomed).