collagenase d (Boehringer Mannheim)

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Boehringer Mannheim collagenase d
Collagenase D, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase d/product/Boehringer Mannheim
Average 92 stars, based on 64 article reviews
Price from $9.99 to $1999.99

collagenase d - by Bioz Stars, 2021-01

92/100 stars

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In Vivo:

Article Title: Near-infrared photoactivatable control of Ca2+ signaling and optogenetic immunomodulation
Article Snippet: .. For in vivo T cell proliferation, spleen and draining popliteal and inguinal LNs were harvested and injected with collagenase D (1 mg/ml; Boehringer-Mannheim, Mannheim, Germany) in RPMI and 10% FBS for 20 min at 37°C. ..

Isolation:

Article Title: Phospholipase C and cAMP-dependent positive inotropic effects of ATP in mouse cardiomyocytes via P2Y11-like receptors
Article Snippet: .. The cells were isolated using perfusion with the same Hepes-buffered solution containing 0.3 mg/ml Collagenase D (0.39 U/mg, Boehringer Mannheim) and 25 μM Ca2+ for 13 min. .. The hearts were thereafter removed and cut into pieces and incubated at 37 °C in the control solution with 100 μM Ca2+ and 1% BSA during shaking for 2–3 min.

Mouse Assay:

Article Title: Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice
Article Snippet: .. For analysis of DCs in NOD.MIP.GFP mice, inguinal and pancreatic lymph nodes were disassociated by ‘teasing’ with a surgical scalpel followed by 20 min of digestion in collagenase D (100 U/ml in Hank's balanced-salt solution; Boehringer Mannheim). .. Cells were then labeled with biotinylated anti-CD11c, followed by streptavidin-allophycocyanin-indotricarbocyanine, anti-CD11b–phycoerythrin, anti-CD3 and anti-B220–peridinin chlorophyll protein and anti-CD8–allophycocyanin.

Concentration Assay:

Article Title: A Prenylation Inhibitor Prevents Production of Infectious Hepatitis Delta Virus Particles
Article Snippet: .. Briefly, an approximate apical piece of liver (3 by 3 by 2 cm; obtained under approved institutional review board protocol) was perfused with 100 ml of preperfusion solution (Earle's balanced salt solution without Ca2+ with 0.7 mM EGTA) for 20 min, followed by perfusion with 80 ml of collagenase D (Boehringer Mannheim) at a concentration of 0.5 mg/ml for 20 min. .. Cells were filtered and hepatocytes were separated from nonparenchymal cells by three rounds of low-speed centrifugation.

Incubation:

Article Title: Cell-Specific Transcription of the Smooth Muscle γ-Actin Gene Requires Both Positive and Negative-Acting cis Elements
Article Snippet: .. Muscular tissue from Hamburger and Hamilton ( ) stage 33–35 (day 8) embryonic gizzards were minced, transferred to medium 199 (Fisher Scientific, Norcross, GA), and dissociated into single cells by sequential incubation in 0.5% collagenase D (Boehringer Mannheim, Indianapolis, IN) at 37°C for 1 h followed by 0.125% trypsin (Gibco BRL, Gaithersburg, MD) treatment at 37°C for 15 min. .. Cells were collected by centrifugation and resuspended in medium 199 supplemented with 20% fetal bovine serum (Hyclone, Logan, UT), 10−6 M insulin, 2 mM L-glutamine, and 100 units/ml penicillin/ streptomycin.

Article Title: Homer Proteins Regulate Coupling of Group I Metabotropic Glutamate Receptors to N-Type Calcium and M-Type Potassium Channels
Article Snippet: .. Briefly, the superior cervical ganglia were dissected from adult rats and incubated in Earle's balanced salt solution (Life Technologies, Rochelle, MD) containing 0.4 mg/ml trypsin (Worthington, Freehold, NJ), 0.6 mg/ml collagenase D (Boehringer Mannheim, Indianapolis, IN), and 0.05 mg/ml DNase I (Sigma, St. Louis, MO) for 1 hr at 35°C. .. Cells were then centrifuged twice, transferred to minimum essential medium (Fisher Scientific, Pittsburgh, PA), plated, and placed in an incubator at 37°C to await DNA injection.

Article Title: Subthreshold Oscillations Induced by Spinal Nerve Injury in Dissociated Muscle and Cutaneous Afferents of Mouse DRG
Article Snippet: .. They were then incubated with gentle shaking in solution D, which consisted of HBSS, 1 mg/ml collagenase D (Boehringer-Mannheim), 0.4 mg/ml 1:250 trypsin (Sigma), and 0.1 mg/ml DNase-1 (Sigma). .. The HBSS contained (in mM) 137 NaCl, 4.2 NaHCO3 , 0.4 Na2 HPO4 , 5.4 KCl, 0.4 KH2 PO4 , 5.5 glucose, and 10 HEPES, pH: 7.3.

Modification:

Article Title: Virus-Like Particles as Carriers for T-Cell Epitopes: Limited Inhibition of T-Cell Priming by Carrier-Specific Antibodies
Article Snippet: .. In brief, lymph nodes (LNs) and spleens were collected and digested twice for 30 min at 37°C in Iscove's modified Dulbecco's medium supplemented with 5% fetal calf serum and 100 μg of collagenase D (Boehringer Mannheim, Mannheim, Germany)/ml. .. Released cells were recovered and resuspended in an Optiprep gradient (Nycomed, Asker, Norway) and centrifuged at 600 × g for 15 min. Low-density cells were collected and stained with phycoerythrin-labeled anti-CD11c and allophycocyanin-labeled anti-CD11b antibodies.