Structured Review

Becton Dickinson collagenase d
Collagenase D, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase d/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
collagenase d - by Bioz Stars, 2021-04
86/100 stars

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Related Articles

Staining:

Article Title: An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium
Article Snippet: 24 h after challenge, mice were killed, and the axillary and inguinal lymph nodes were removed. .. Tissue was disrupted by 0.5 mg/ml collagenase D (Roche) for 30 min at 37°C, passed through a 70-μm cell strainer (BD Biosciences), and stained with PE anti–mouse CD11c (BD Biosciences) for FACS analysis. .. To observe blocking of DC migration from the dermis, contact hypersensitivity was induced by oxazolone in BALB/c male mice by application to the abdomen.

FACS:

Article Title: An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium
Article Snippet: 24 h after challenge, mice were killed, and the axillary and inguinal lymph nodes were removed. .. Tissue was disrupted by 0.5 mg/ml collagenase D (Roche) for 30 min at 37°C, passed through a 70-μm cell strainer (BD Biosciences), and stained with PE anti–mouse CD11c (BD Biosciences) for FACS analysis. .. To observe blocking of DC migration from the dermis, contact hypersensitivity was induced by oxazolone in BALB/c male mice by application to the abdomen.

Incubation:

Article Title: CD4+ T-cell-dependent tumour rejection in an immune-privileged environment requires macrophages
Article Snippet: .. Spleen cells were incubated at 37° for 30 min with 1 mg/ml collagenase D (Roche, Indianapolis, IN), then plated on Primaria™ tissue culture dishes (BD Biosciences) and incubated for 4 hr at 37°. ..

Article Title: Psychological stress impairs the local CD8+ T cell response to mucosal HSV-1 infection and allows for increased pathogenicity via a glucocorticoid receptor-mediated mechanism
Article Snippet: An incision was made along the toothline with a scalpel and the palate was peeled back from the incisors toward the molars and placed in Iscove’s-modified Dulbecco’s media (IMDM) (Gibco; Carlsbad, CA) supplemented with 10% (v/v) fetal bovine serum (FBS), 50 µM 2-mercaptoethanol, 2 mM glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. .. To dissociate lymphoid cells from the palates, the palates were incubated in 2 mg/mL collagenase D (Roche, Indianapolis, IN) for 40 minutes at 37°C and then rubbed against a 70 µm nylon cell strainer (BD Biosciences, Bedford, MA). ..

Cell Isolation:

Article Title: Interleukin-1 receptor antagonist prevents murine bronchopulmonary dysplasia induced by perinatal inflammation and hyperoxia
Article Snippet: Proteome Profiler Arrays (R & D Systems) were used as described previously ( ). .. For lung cell isolation on days 14 and 28, the left lung was minced and digested with collagenase D (1 mg/mL) at 37 °C for 30 min. After digestion, lung homogenates were macerated through a 70-μM cell strainer, red blood cells were lysed (BD Pharm Lyse) for 2× 10 min, then centrifuged at 400 × g for 10 min at 18 °C. .. Pellets were washed twice with PBS and cells were stained and acquired using a FACS Canto II flow cytometer (BD).

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    Becton Dickinson collagenase d
    Collagenase D, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase d/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase d - by Bioz Stars, 2021-04
    86/100 stars
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