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Becton Dickinson collagenase d
Collagenase D, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase d/product/Becton Dickinson
Average 92 stars, based on 19 article reviews
Price from $9.99 to $1999.99
collagenase d - by Bioz Stars, 2021-01
92/100 stars

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Related Articles

Flow Cytometry:

Article Title: Dendritic cell MST1 inhibits Th17 differentiation
Article Snippet: .. Cell cultures and flow cytometry Spleens were digested with Collagenase D and DCs (CD11c+ TCR− CD19− NK1.1− F4/80− Ly6G− ) were sorted on a FACS caliber (Becton Dickinson, CA, USA). ..

Article Title: Leukemia-associated activating mutation of Flt3 expands dendritic cells and alters T cell responses
Article Snippet: .. BrdU analysis Flt3+/+ or Flt3ITD/+ mice were pulsed with an initial intraperitoneal injection with 1 mg BrdU (Sigma-Aldrich), followed by a continuous administration of 0.8 mg/ml BrdU in the drinking water for 2 d. Spleens were harvested after 2 d, treated with collagenase D (1 mg/ml) and DNase I (20 µg/ml), and analyzed for BrdU incorporation using the APC BrdU Flow kit (BD) per the manufacturer’s instructions. .. RNA isolation and gene expression analysis Cells were sorted from splenocytes pooled from two to three animals of each genotype, and samples from independent experiments were used as biological replicates in RNA-Seq (three for CD8+ cDCs, two for CD11b+ cDCs, and two for pDCs).

Isolation:

Article Title: Immunomodulatory tetracyclines shape the intestinal inflammatory response inducing mucosal healing and resolution
Article Snippet: .. Cells from the colonic lamina propria (cLP) were isolated as described previously (Scott et al ., ) using a digestion media composed of HBSS without Mg2+ or Ca2+ , 10% of FBS and 0.5 mg·mL−1 collagenase V (Sigma‐Aldrich Quimica, Madrid, Spain), 0.65 mg·mL−1 collagenase D, 30 μg·mL−1 DNase I and 1 mg·mL−1 (50 ng·mL−1 ) and ionomycin (1 μg·mL−1 (RA3‐6B2, BD Bioscience, San Jose, CA, United States), α‐mouse CD3 (17A2), α‐mouse CD8 (53–6.7), α‐mouse CD4 (RM4–5, BD Bioscience), α‐mouse IL‐4 (BVD4‐1D11), α‐mouse IFNγ (XMG1.2, BD Pharmigen), α‐mouse IL‐17A (eBio17B7, eBioscience) and α‐mouse FoxP3 (FJK‐16s, eBioscience). .. Samples were acquired using a FACSVerse™ or FACSCanto II™ cytometers (Becton Dickinson, USA), and data were analysed using FlowJo software (Tree Star, USA).

Transferring:

Article Title: Reduced chondrocyte proliferation and chondrodysplasia in mice lacking the integrin-linked kinase in chondrocytes
Article Snippet: .. The denser cartilage was allowed to sediment, and the soft tissues were aspirated; the cartilage was then further digested with collagenase D for 3 h. Undigested bony parts were discarded, and the remaining cell suspension was aspirated repeatedly with a Pasteur pipette, filtered through a 70-μm cell strainer (Falcon; Becton Dickinson), rinsed first in PBS then in serum-free DMEM, and counted. ..

Cytometry:

Article Title: Dendritic cell MST1 inhibits Th17 differentiation
Article Snippet: .. Cell cultures and flow cytometry Spleens were digested with Collagenase D and DCs (CD11c+ TCR− CD19− NK1.1− F4/80− Ly6G− ) were sorted on a FACS caliber (Becton Dickinson, CA, USA). ..

BrdU Incorporation Assay:

Article Title: Leukemia-associated activating mutation of Flt3 expands dendritic cells and alters T cell responses
Article Snippet: .. BrdU analysis Flt3+/+ or Flt3ITD/+ mice were pulsed with an initial intraperitoneal injection with 1 mg BrdU (Sigma-Aldrich), followed by a continuous administration of 0.8 mg/ml BrdU in the drinking water for 2 d. Spleens were harvested after 2 d, treated with collagenase D (1 mg/ml) and DNase I (20 µg/ml), and analyzed for BrdU incorporation using the APC BrdU Flow kit (BD) per the manufacturer’s instructions. .. RNA isolation and gene expression analysis Cells were sorted from splenocytes pooled from two to three animals of each genotype, and samples from independent experiments were used as biological replicates in RNA-Seq (three for CD8+ cDCs, two for CD11b+ cDCs, and two for pDCs).

FACS:

Article Title: Dendritic cell MST1 inhibits Th17 differentiation
Article Snippet: .. Cell cultures and flow cytometry Spleens were digested with Collagenase D and DCs (CD11c+ TCR− CD19− NK1.1− F4/80− Ly6G− ) were sorted on a FACS caliber (Becton Dickinson, CA, USA). ..

Injection:

Article Title: Leukemia-associated activating mutation of Flt3 expands dendritic cells and alters T cell responses
Article Snippet: .. BrdU analysis Flt3+/+ or Flt3ITD/+ mice were pulsed with an initial intraperitoneal injection with 1 mg BrdU (Sigma-Aldrich), followed by a continuous administration of 0.8 mg/ml BrdU in the drinking water for 2 d. Spleens were harvested after 2 d, treated with collagenase D (1 mg/ml) and DNase I (20 µg/ml), and analyzed for BrdU incorporation using the APC BrdU Flow kit (BD) per the manufacturer’s instructions. .. RNA isolation and gene expression analysis Cells were sorted from splenocytes pooled from two to three animals of each genotype, and samples from independent experiments were used as biological replicates in RNA-Seq (three for CD8+ cDCs, two for CD11b+ cDCs, and two for pDCs).

Mouse Assay:

Article Title: Leukemia-associated activating mutation of Flt3 expands dendritic cells and alters T cell responses
Article Snippet: .. BrdU analysis Flt3+/+ or Flt3ITD/+ mice were pulsed with an initial intraperitoneal injection with 1 mg BrdU (Sigma-Aldrich), followed by a continuous administration of 0.8 mg/ml BrdU in the drinking water for 2 d. Spleens were harvested after 2 d, treated with collagenase D (1 mg/ml) and DNase I (20 µg/ml), and analyzed for BrdU incorporation using the APC BrdU Flow kit (BD) per the manufacturer’s instructions. .. RNA isolation and gene expression analysis Cells were sorted from splenocytes pooled from two to three animals of each genotype, and samples from independent experiments were used as biological replicates in RNA-Seq (three for CD8+ cDCs, two for CD11b+ cDCs, and two for pDCs).

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    Becton Dickinson collagenase d undigested bony tissues
    Collagenase D Undigested Bony Tissues, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase d undigested bony tissues/product/Becton Dickinson
    Average 91 stars, based on 1 article reviews
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    Becton Dickinson collagenase d
    Collagenase D, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase d/product/Becton Dickinson
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    collagenase d - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    91
    Becton Dickinson collagenase d treatment
    Collagenase D Treatment, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
    collagenase d treatment - by Bioz Stars, 2021-01
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