collagenase cls type ii (Worthington Biochemical)

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Name:

Collagenase Type 2

Description:

Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder

Catalog Number:

ls004174

Price:

Source:

Clostridium histolyticum

Size:

100 mg

Cas Number:

9001.12.1

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Structured Review

Worthington Biochemical collagenase cls type ii
Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
https://www.bioz.com/result/collagenase cls type ii/product/Worthington Biochemical
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

collagenase cls type ii - by Bioz Stars, 2021-03

99/100 stars

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In Vitro:

Article Title: Development of A Decellularized Meniscus Matrix-Based Nanofibrous Scaffold for Meniscus Tissue Engineering
Article Snippet: .. 2.5 In vitro Enzymatic Degradation of dMEP NanofibersTo explore the degradation behavior of dMEP scaffolds, acellular PCL and GA or GP crosslinked dMEP scaffolds (n = 4/group) were digested in 2 mg/mL collagenase type 2 solution (Worthington) for 24 h at 37°C, and then digested in 100 ug/mL proteinase K in tris-HCl overnight at 60°C. .. A scaffold from each group was used for SEM imaging to visualize changes in fiber morphology and structure after enzymatic degradation, and the remaining were used for the orthohydroxyproline (OHP) assay to quantify remaining collagen [ ].

FACS:

Article Title: Breast cancer endocrine therapy exhausts adipocyte progenitors promoting weight gain and glucose intolerance
Article Snippet: Proteins were detected by Li-COR (Odyssey CLX) Western blot scanner, and densitometric analysis was performed using Image Studio v4.1. .. Adipose Tissue FACS Whole inguinal subcutaneous adipose depots were excised, minced briefly (5 min) with dissecting scissors, and digested for 75 mins at 37C in a collagenase solution (Collagenase type II, Worthington LS004177). (HBSS with 3% BSA, 0.8mM ZnCl2, Mg, Ca, 0.8mg/ml collagenase). .. Stromal pellets were incubated in red blood cell lysis buffer (Sigma Aldrich), washed, and stained with fluorescent-conjugated antibodies for CD45, CD31, Sca1, CD29, CD34, and CD24 as described .

Incubation:

Article Title: Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells
Article Snippet: Skin samples were incubated in 2 mg/ml dispase (383-02281; Wako Pure Chemical Industries, Osaka, Japan) and were separated into epidermis and dermis. .. Dermis was minced and then incubated with 2 mg/ml collagenase type 2 (CLS-2; Worthington Biochemical, Lakewood, NJ, USA) in Tyrode’s solution for 60–90 minutes. ..

Mouse Assay:

Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart
Article Snippet: The protocol is based on that originally developed by the Alliance for Cell Signalling (AfCS) for adult mouse cardiomyocyte isolation , with some minor modifications. .. Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington). ..