cls 1  (Worthington Biochemical)


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  • 99
    Name:
    Collagenase Type 1
    Description:
    The original balance of enzymatic activities Each lot assayed for collagenase caseinase clostripain and tryptic activities Suggested for epithelial liver lung and adrenal primary cell isolations A dialyzed lyophilized powder
    Catalog Number:
    LS004194
    Price:
    35
    Source:
    Clostridium histolyticum
    Size:
    100 mg
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical cls 1
    The original balance of enzymatic activities Each lot assayed for collagenase caseinase clostripain and tryptic activities Suggested for epithelial liver lung and adrenal primary cell isolations A dialyzed lyophilized powder
    https://www.bioz.com/result/cls 1/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cls 1 - by Bioz Stars, 2021-06
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Tbx1 regulates inherited metabolic and myogenic abilities of progenitor cells derived from slow- and fast-type muscle
    Article Snippet: The protein content was measured using a PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). .. Isolation and culture of single muscle fibersThe EDL, TA, and SOL muscles isolated from mice were digested with 2 mg/ml collagenase type I (Worthington Biochemical), as previously described [ ], and the isolated muscle fibers were suspended and cultured in growth medium (DMEM containing 15% horse serum [Invitrogen] with 0.5% chick embryo extract [Gemini Bio-Products, West Sacramento, CA] and penicillin–streptomycin) at 37 °C [ ]. .. EdU (Invitrogen) was added to the culture medium at a final concentration of 10 μM and cultured for 24 h.

    Article Title: VEGFR2 signaling drives meningeal vascular regeneration upon head injury
    Article Snippet: .. Magnetic-associated and fluorescence-activated cell sorting To sort dura mater or brain ECs, CD45+ hematopoietic or CD45− non-hematopoietic cells by FACS, tissues were isolated as described above and dissociated in collagenase type I (1 mg/ml, Worthington), dispase (1 mg/ml, Gibco), and DNase I (0.1 mg/ml, Merck) dissolved in DMEM/F12 supplemented with 5% fetal bovine serum (FBS) at 37 °C for 30 min. .. Cell suspensions went on RBC lysis by suspension in ACK lysis buffer (Gibco) for 5 min on ice and blocked with mouse anti-CD16/CD32 (553141, BD Bioscience) before being incubated for 20 min with indicated antibodies in FACS buffer (2% FBS in PBS).

    Mouse Assay:

    Article Title: Tbx1 regulates inherited metabolic and myogenic abilities of progenitor cells derived from slow- and fast-type muscle
    Article Snippet: The protein content was measured using a PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). .. Isolation and culture of single muscle fibersThe EDL, TA, and SOL muscles isolated from mice were digested with 2 mg/ml collagenase type I (Worthington Biochemical), as previously described [ ], and the isolated muscle fibers were suspended and cultured in growth medium (DMEM containing 15% horse serum [Invitrogen] with 0.5% chick embryo extract [Gemini Bio-Products, West Sacramento, CA] and penicillin–streptomycin) at 37 °C [ ]. .. EdU (Invitrogen) was added to the culture medium at a final concentration of 10 μM and cultured for 24 h.

    Cell Culture:

    Article Title: Tbx1 regulates inherited metabolic and myogenic abilities of progenitor cells derived from slow- and fast-type muscle
    Article Snippet: The protein content was measured using a PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). .. Isolation and culture of single muscle fibersThe EDL, TA, and SOL muscles isolated from mice were digested with 2 mg/ml collagenase type I (Worthington Biochemical), as previously described [ ], and the isolated muscle fibers were suspended and cultured in growth medium (DMEM containing 15% horse serum [Invitrogen] with 0.5% chick embryo extract [Gemini Bio-Products, West Sacramento, CA] and penicillin–streptomycin) at 37 °C [ ]. .. EdU (Invitrogen) was added to the culture medium at a final concentration of 10 μM and cultured for 24 h.

    Fluorescence:

    Article Title: VEGFR2 signaling drives meningeal vascular regeneration upon head injury
    Article Snippet: .. Magnetic-associated and fluorescence-activated cell sorting To sort dura mater or brain ECs, CD45+ hematopoietic or CD45− non-hematopoietic cells by FACS, tissues were isolated as described above and dissociated in collagenase type I (1 mg/ml, Worthington), dispase (1 mg/ml, Gibco), and DNase I (0.1 mg/ml, Merck) dissolved in DMEM/F12 supplemented with 5% fetal bovine serum (FBS) at 37 °C for 30 min. .. Cell suspensions went on RBC lysis by suspension in ACK lysis buffer (Gibco) for 5 min on ice and blocked with mouse anti-CD16/CD32 (553141, BD Bioscience) before being incubated for 20 min with indicated antibodies in FACS buffer (2% FBS in PBS).

    FACS:

    Article Title: VEGFR2 signaling drives meningeal vascular regeneration upon head injury
    Article Snippet: .. Magnetic-associated and fluorescence-activated cell sorting To sort dura mater or brain ECs, CD45+ hematopoietic or CD45− non-hematopoietic cells by FACS, tissues were isolated as described above and dissociated in collagenase type I (1 mg/ml, Worthington), dispase (1 mg/ml, Gibco), and DNase I (0.1 mg/ml, Merck) dissolved in DMEM/F12 supplemented with 5% fetal bovine serum (FBS) at 37 °C for 30 min. .. Cell suspensions went on RBC lysis by suspension in ACK lysis buffer (Gibco) for 5 min on ice and blocked with mouse anti-CD16/CD32 (553141, BD Bioscience) before being incubated for 20 min with indicated antibodies in FACS buffer (2% FBS in PBS).

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