Journal: Journal of Negative Results in Biomedicine
Article Title: Effects of antibody to receptor activator of nuclear factor κ-B ligand on inflammation and cartilage degradation in collagen antibody-induced arthritis in mice
Figure Lengend Snippet: Histological analyses of limb joints. The 4 limbs were excised from all mice at 14 days after injection of a cocktail of antibodies to type II collagen. Decalcified 8-μm sections of hands (A-D) , elbows (E-F) , toes (I-L) , and knees (M-P) from RA-/Ab- (A , E , I , M) , RA-/Ab+ (B , F , J , N) , RA+/Ab- (C , G , K , O) , and RA+/Ab+ (D , H , L , P) mice were stained with hematoxylin and eosin. Synovial thickening and accumulation of inflammatory cells were observed in joints from the RA+ mice (arrowheads in panels C , D , G , H , K , L . O , and P ). Bars: 500 μm (A-D , I-P ) and 200 μm (E-H) .
Article Snippet: DBA1/J mice in the RA+ groups were given an intra-peritoneal injection of a cocktail of 5 clones of mouse monoclonal anti-type II collagen antibodies (Chondrex) on day 0, followed by an intra-peritoneal injection of E. coli LPS on day 3.
Techniques: Mouse Assay, Injection, Staining