collagen type ii  (Chondrex inc)

Journal: BMC Musculoskeletal Disorders

Article Title: Repository corticotropin injection attenuates collagen-induced arthritic joint structural damage and has enhanced effects in combination with etanercept

doi: 10.1186/s12891-020-03609-3

Figure Lengend Snippet: Ankle diameter measurements in rat CIA. Collagen type-II immunization of dark Agouti rats led to increased ankle diameter (mean ± SEM, n = 10 per group) by day 13, and these clinical parameters continued to worsen until reaching a plateau on days 18 through 20. The disease-induced increase in ankle diameter was generally dose-dependently attenuated by RCI (160 and 400 U/kg) versus the disease control group when administered after disease onset (beginning day 13). Treatment with ETN (10 mg/kg) also significantly decreased ankle diameter compared with the disease control. When administered in combination with ETN, 160 or 400 U/kg RCI further reduced ankle diameter vs ETN alone. Statistics were analyzed by 2-way ANOVA followed by a Newman-Keuls multiple comparisons test. All p values represent treatment compared with disease control. Abbreviations: ANOVA, analysis of variance; CIA, collagen-induced arthritis; ETN, etanercept; RCI, repository corticotropin injection; SEM, standard error of the mean

Article Snippet: Rats were anesthetized with isoflurane, which has been shown to have a minimal effect on inflammation, and injected with 400 μL of Freund’s incomplete adjuvant (Difco, Detroit, MI) emulsified with 2 mg/mL porcine type II collagen (Chondrex, Inc., Redmond, WA) at 2 sites on the back (200 μL per site) on day 0, and then 100 μL at 1 site on day 7.

Techniques: Injection

Journal: Journal of Negative Results in Biomedicine

Article Title: Effects of antibody to receptor activator of nuclear factor κ-B ligand on inflammation and cartilage degradation in collagen antibody-induced arthritis in mice

doi: 10.1186/s12952-014-0018-0

Figure Lengend Snippet: Change in arthritis score after injection of antibodies to type II collagen. Arthritis scores were determined daily after injection of a cocktail of antibodies to type II collagen according to the following criteria: 0, normal; 1, mild but definite redness and swelling of the ankle or wrist, or apparent redness and swelling limited to individual digits regardless of the number of affected digits; 2, moderate redness and swelling of ankle and wrist; 3, severe redness and swelling of the entire paw including digits; 4, maximally inflamed limb with involvement of multiple joints. Data are expressed as the mean ± SD. There was no statistical difference between RA+/Ab- group and RA+/Ab+ group (Steel-Dwass test).

Article Snippet: DBA1/J mice in the RA+ groups were given an intra-peritoneal injection of a cocktail of 5 clones of mouse monoclonal anti-type II collagen antibodies (Chondrex) on day 0, followed by an intra-peritoneal injection of E. coli LPS on day 3.

Techniques: Injection

Journal: Journal of Negative Results in Biomedicine

Article Title: Effects of antibody to receptor activator of nuclear factor κ-B ligand on inflammation and cartilage degradation in collagen antibody-induced arthritis in mice

doi: 10.1186/s12952-014-0018-0

Figure Lengend Snippet: Histological analyses of limb joints. The 4 limbs were excised from all mice at 14 days after injection of a cocktail of antibodies to type II collagen. Decalcified 8-μm sections of hands (A-D) , elbows (E-F) , toes (I-L) , and knees (M-P) from RA-/Ab- (A , E , I , M) , RA-/Ab+ (B , F , J , N) , RA+/Ab- (C , G , K , O) , and RA+/Ab+ (D , H , L , P) mice were stained with hematoxylin and eosin. Synovial thickening and accumulation of inflammatory cells were observed in joints from the RA+ mice (arrowheads in panels C , D , G , H , K , L . O , and P ). Bars: 500 μm (A-D , I-P ) and 200 μm (E-H) .

Article Snippet: DBA1/J mice in the RA+ groups were given an intra-peritoneal injection of a cocktail of 5 clones of mouse monoclonal anti-type II collagen antibodies (Chondrex) on day 0, followed by an intra-peritoneal injection of E. coli LPS on day 3.

Techniques: Mouse Assay, Injection, Staining

Journal: Journal of Negative Results in Biomedicine

Article Title: Effects of antibody to receptor activator of nuclear factor κ-B ligand on inflammation and cartilage degradation in collagen antibody-induced arthritis in mice

doi: 10.1186/s12952-014-0018-0

Figure Lengend Snippet: Experimental design. Eight-week-old male DBA1/J mice were divided into 4 groups (6 in each), i.e., RA-/Ab-, RA-/Ab+, RA+/Ab-, and RA+/Ab+. A cocktail of anti-type II collagen antibodies and E. coli LPS was injected intra-peritoneally into the RA+ mice on days 0 and 3, respectively. RA- mice were the control without injection of the anti-type II collagen antibodies and LPS. The OYC1 anti-RANKL monoclonal antibody (5 mg/kg) was injected subcutaneously into the Ab+ mice on day 5. Ab- mice were the control without injection the anti-RANKL antibody. Arthritis scores were determined daily from day 0 to 14. The 4 limbs were removed on day 14 for analysis of histological changes in the joints.

Article Snippet: DBA1/J mice in the RA+ groups were given an intra-peritoneal injection of a cocktail of 5 clones of mouse monoclonal anti-type II collagen antibodies (Chondrex) on day 0, followed by an intra-peritoneal injection of E. coli LPS on day 3.

Techniques: Mouse Assay, Injection

Journal: Pharmaceutical Biology

Article Title: Alantolactone alleviates collagen-induced arthritis and inhibits Th17 cell differentiation through modulation of STAT3 signalling

doi: 10.1080/13880209.2021.1876102

Figure Lengend Snippet: Alantolactone suppressed type II collagen (CII)-specific immune responses in mice with CIA. (A) Forty-two days postimmunization, serum was obtained from alantolactone- or vehicle-treated mice, and anti-CII IgG was measured by ELISA at a 1:2500 dilution. (B) Spleens were harvested at day 42 postimmunization and tested for their ability to proliferate in vitro for 72 h in medium containing CII (50 μg/mL). The proliferation of the spleen was measured by 3 H-TdR incorporation, and the stimulation index was calculated. Bar graphs representative of three independent experiments. Values are expressed as the mean ± SEM ( n = 6 per group). ** p

Article Snippet: Briefly, 2 mg/mL bovine type II collagen (CII, Chondrex, Redmond, WA) was dissolved in 10 mM acetic acid and emulsified at a 1:1 ratio with complete Freund’s adjuvant (CFA, Chondrex, Redmond, WA).

Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, In Vitro

Journal: International Journal of Molecular Sciences

Article Title: Targeting of Phospholipase D1 Ameliorates Collagen-Induced Arthritis via Modulation of Treg and Th17 Cell Imbalance and Suppression of Osteoclastogenesis

doi: 10.3390/ijms21093230

Figure Lengend Snippet: The PLD1 inhibitor reduces the production of the anti-CII IgG2a autoantibody and proinflammatory cytokines in collagen-induced arthritis (CIA) mice. ( A ) The amount of anti-collagen IgG1 and IgG2a antibodies was measured by ELISA in the serum of the indicated mice at day 45. ( B ) The amount of proinflammatory cytokines was measured by ELISA in the serum of the indicated mice at day 45. n = 15 per group. * p

Article Snippet: Measurement of Type II Collagen-Specific AutoantibodiesThe amount of murine CII-specific autoantibodies in the serum was measured using the Mouse Anti-Type II Collagen IgG Subtype Antibody Assay Kit (Chondrex), according to the manufacturer’s instructions.

Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay