coli top10 f  (Thermo Fisher)


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    Structured Review

    Thermo Fisher coli top10 f
    Coli Top10 F, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli top10 f/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    coli top10 f - by Bioz Stars, 2020-04
    94/100 stars

    Related Products / Commonly Used Together

    fhu
    ompw
    cloning vectors puc57

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    Related Articles

    Clone Assay:

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: .. Bacterial strains, plasmids and culture conditions E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. E . coli clones were incubated at 37°C and 250 rpm in TSB (Tryptic Soy Broth, Merck) or LB (Luria-Bertani Broth, Merck) for growing in liquid medium, and TSB containing agar was used for growing on solid medium.

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: .. E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. E . coli clones were incubated at 37°C and 250 rpm in TSB (Tryptic Soy Broth, Merck) or LB (Luria-Bertani Broth, Merck) for growing in liquid medium, and TSB containing agar was used for growing on solid medium.

    Selection:

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. Appropriate concentrations of antibiotics were added for plasmid selection: 100 μg/mL ampicillin for both vectors.

    Subcloning:

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: .. Bacterial strains, plasmids and culture conditions E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. E . coli clones were incubated at 37°C and 250 rpm in TSB (Tryptic Soy Broth, Merck) or LB (Luria-Bertani Broth, Merck) for growing in liquid medium, and TSB containing agar was used for growing on solid medium.

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: .. E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. E . coli clones were incubated at 37°C and 250 rpm in TSB (Tryptic Soy Broth, Merck) or LB (Luria-Bertani Broth, Merck) for growing in liquid medium, and TSB containing agar was used for growing on solid medium.

    Incubation:

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: Bacterial strains, plasmids and culture conditions E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. E . coli clones were incubated at 37°C and 250 rpm in TSB (Tryptic Soy Broth, Merck) or LB (Luria-Bertani Broth, Merck) for growing in liquid medium, and TSB containing agar was used for growing on solid medium.

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. E . coli clones were incubated at 37°C and 250 rpm in TSB (Tryptic Soy Broth, Merck) or LB (Luria-Bertani Broth, Merck) for growing in liquid medium, and TSB containing agar was used for growing on solid medium.

    Expressing:

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: .. Bacterial strains, plasmids and culture conditions E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. E . coli clones were incubated at 37°C and 250 rpm in TSB (Tryptic Soy Broth, Merck) or LB (Luria-Bertani Broth, Merck) for growing in liquid medium, and TSB containing agar was used for growing on solid medium.

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: .. E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. E . coli clones were incubated at 37°C and 250 rpm in TSB (Tryptic Soy Broth, Merck) or LB (Luria-Bertani Broth, Merck) for growing in liquid medium, and TSB containing agar was used for growing on solid medium.

    Plasmid Preparation:

    Article Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
    Article Snippet: E . coli TOP10 F’ (Invitrogen, a strain with wt genes coding for for Fhu and OmpW), and cloning vectors pUC57 (GenScript) and pIAGO [ ] were used for routine sub-cloning while Streptomyces albus was used as host for heterologous protein expression. .. Appropriate concentrations of antibiotics were added for plasmid selection: 100 μg/mL ampicillin for both vectors.

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  • 99
    Thermo Fisher competent e coli one shot top10 cells
    Competent E Coli One Shot Top10 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher top10 f e coli
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    Thermo Fisher competent e coli dh10β top10 f
    Competent E Coli Dh10β Top10 F, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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