coli bl21 de3 rosetta strain  (Millipore)


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    Structured Review

    Millipore coli bl21 de3 rosetta strain
    Coli Bl21 De3 Rosetta Strain, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli bl21 de3 rosetta strain/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    coli bl21 de3 rosetta strain - by Bioz Stars, 2020-03
    85/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: Paragraph title: Protein cloning, expression and purification ... Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media.

    Amplification:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: Protein cloning, expression and purification YLR456W ORF was amplified from Saccharomyces cerevisiae BY4741 strain cDNA by PCR using the following primers pairs: 5’ CAT ATG AAA CTC AAT GAA CAA ATA CCA AA 3’and 5’ GGA TCC TTA AAC TGG TTG TAC TG 3’containg Nde I and BamH I restrictions sites (in bold). .. Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media.

    Synthesized:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: YPR172W and PDX3 sequences were obtained from Saccharomyces Genome Data Base (strain S288C), synthesized and cloned into pET28a using Nde I and BamH I restriction sites (GenScript Company). .. Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media.

    Protease Inhibitor:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media. .. Cellular lysis was performed in buffer A, 20 mM Tris, 150 mM NaCl, 1 mM DTT pH 7.4 in the presence of a protease inhibitor cocktail (Roche).

    Construct:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: These constructs code for proteins containing an N-terminal His-tag followed by a thrombin digestion site. .. Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media.

    Purification:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: Paragraph title: Protein cloning, expression and purification ... Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media.

    Expressing:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: .. Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media. .. When the culture reached OD600nm = 0.5, expression was induced with 1 mM IPTG for 5 hours at 37°C.

    Polymerase Chain Reaction:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: The PCR product was cloned into the pET28a plasmid (Novagen). .. Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media.

    Lysis:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media. .. Cellular lysis was performed in buffer A, 20 mM Tris, 150 mM NaCl, 1 mM DTT pH 7.4 in the presence of a protease inhibitor cocktail (Roche).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: Protein cloning, expression and purification YLR456W ORF was amplified from Saccharomyces cerevisiae BY4741 strain cDNA by PCR using the following primers pairs: 5’ CAT ATG AAA CTC AAT GAA CAA ATA CCA AA 3’and 5’ GGA TCC TTA AAC TGG TTG TAC TG 3’containg Nde I and BamH I restrictions sites (in bold). .. Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media.

    Cellular Antioxidant Activity Assay:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: Protein cloning, expression and purification YLR456W ORF was amplified from Saccharomyces cerevisiae BY4741 strain cDNA by PCR using the following primers pairs: 5’ CAT ATG AAA CTC AAT GAA CAA ATA CCA AA 3’and 5’ GGA TCC TTA AAC TGG TTG TAC TG 3’containg Nde I and BamH I restrictions sites (in bold). .. Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media.

    Plasmid Preparation:

    Article Title: Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi
    Article Snippet: The PCR product was cloned into the pET28a plasmid (Novagen). .. Protein expression was performed with E . coli BL21 DE3 Rosetta strain (Novagen) grown in LB media.

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