coli bl21 de3 rosetta strain  (Millipore)


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    Name:
    BL21 DE3 Chemically Competent Cells
    Description:
    The BL21 DE3 Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG inducible promoter for use with any expression plasmid containing the T7 promoter
    Catalog Number:
    cmc0014
    Price:
    None
    Applications:
    The E. coli strain BL21 (DE3) has been used to express tau 2N4R isoform (aa1-441) and the truncated tau (aa1-421) using pET19b as expression vector.
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    Structured Review

    Millipore coli bl21 de3 rosetta strain
    BL21 DE3 Chemically Competent Cells
    The BL21 DE3 Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG inducible promoter for use with any expression plasmid containing the T7 promoter
    https://www.bioz.com/result/coli bl21 de3 rosetta strain/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    coli bl21 de3 rosetta strain - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Electroporation:

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli
    Article Snippet: .. Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes. .. These recombinant strains were subsequently transformed using the helper plasmids pBB540/pBB542 or pTUM4.1.

    Positron Emission Tomography:

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli
    Article Snippet: .. Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes. .. These recombinant strains were subsequently transformed using the helper plasmids pBB540/pBB542 or pTUM4.1.

    Article Title: YeeV is an Escherichia coli Toxin that Inhibits Cell Division by Targeting the Cytoskeleton Proteins, FtsZ and MreB
    Article Snippet: .. E. coli BW25113 ( ΔaraBD ) (Datsenko and Wanner, 2000), BL21 (DE3) were grown in M9 medium at 37° C. Plasmids pET-28a- yeeV , pET-28a- ftsZ , pET-28a- mreB and pET-28a -sulA were constructed in pET-28a (Novagen) with an N-terminal His6 tag. .. Plasmids pBAD33- yeeV and pBAD33- sulA were constructed from pBAD33 (Guzman et al., 1995) to tightly regulate gene expression by the addition of arabinose.

    Construct:

    Article Title: YeeV is an Escherichia coli Toxin that Inhibits Cell Division by Targeting the Cytoskeleton Proteins, FtsZ and MreB
    Article Snippet: .. E. coli BW25113 ( ΔaraBD ) (Datsenko and Wanner, 2000), BL21 (DE3) were grown in M9 medium at 37° C. Plasmids pET-28a- yeeV , pET-28a- ftsZ , pET-28a- mreB and pET-28a -sulA were constructed in pET-28a (Novagen) with an N-terminal His6 tag. .. Plasmids pBAD33- yeeV and pBAD33- sulA were constructed from pBAD33 (Guzman et al., 1995) to tightly regulate gene expression by the addition of arabinose.

    Article Title: Characterization of gene expression on genomic segment 7 of infectious salmon anaemia virus
    Article Snippet: .. The plasmid constructs were used to transform competent E. coli strain BL21(DE3)(pLysS) (Novagen) in order to express the GST fusion proteins. ..

    Purification:

    Article Title: Localization of a Class III Myosin to Filopodia Tips in Transfected HeLa Cells Requires an Actin-binding Site in its Tail Domain
    Article Snippet: .. Recombinant GST proteins were expressed from pGEX (Pharmacia) vectors in BL21[DE3] E. coli cell line and purified using glutathione-agarose beads (Sigma, St. Louis, MO) per manufacturers protocol. .. Actin filaments were formed in vitro from mammalian actin monomers as described ( ).

    Expressing:

    Article Title: Fatty acid DSF binds and allosterically activates histidine kinase RpfC of phytopathogenic bacterium Xanthomonas campestris pv. campestris to regulate quorum-sensing and virulence
    Article Snippet: .. E . coli BL21(DE3) strain was used as the host for expressing recombinant proteins with pET30a vector (Novagen, USA). ..

    Article Title: Immunization with 3-oxododecanoyl-L-homoserine lactone-r-PcrV conjugate enhances survival of mice against lethal burn infections caused by Pseudomonas aeruginosa
    Article Snippet: .. Escherichia (E.) coli strains DH5a and Bl21 (DE3) (Novagen Co., Wisconsin, USA) were used for clone and expression of recombinant PcrV protein (r-PcrV), respectively. .. Luria-Bertani (LB) broth, agar (Merck Co., Germany) and Pseudomonas Selective Agar were used for the bacterial culturing.

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli
    Article Snippet: .. Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes. .. These recombinant strains were subsequently transformed using the helper plasmids pBB540/pBB542 or pTUM4.1.

    Transformation Assay:

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli
    Article Snippet: .. Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes. .. These recombinant strains were subsequently transformed using the helper plasmids pBB540/pBB542 or pTUM4.1.

    Recombinant:

    Article Title: Expression and Functional Characterization of Membrane-Integrated Mammalian Corticotropin Releasing Factor Receptors 1 and 2 in Escherichia coli
    Article Snippet: .. For recombinant protein production: BL21(DE3) [F− ompT hsdSB (rB − mB − ) gal dcm (DE3)] (Novagen), OverExpress C41(DE3) and C43(DE3), which are derivative of BL21(DE3) containing genetic mutations phenotypically selected for conferring tolerance to toxic proteins (Lucigen), and Rosetta2(DE3) [F− ompT hsdS B (rB − mB − ) gal dcm (DE3) pRARE2 (CamR )] (Novagen) providing tRNAs for seven E. coli rare codons. .. For DNA vectors cloning and propagation: One Shot® TOP10 (Life Technologies).

    Article Title: Fatty acid DSF binds and allosterically activates histidine kinase RpfC of phytopathogenic bacterium Xanthomonas campestris pv. campestris to regulate quorum-sensing and virulence
    Article Snippet: .. E . coli BL21(DE3) strain was used as the host for expressing recombinant proteins with pET30a vector (Novagen, USA). ..

    Article Title: Immunization with 3-oxododecanoyl-L-homoserine lactone-r-PcrV conjugate enhances survival of mice against lethal burn infections caused by Pseudomonas aeruginosa
    Article Snippet: .. Escherichia (E.) coli strains DH5a and Bl21 (DE3) (Novagen Co., Wisconsin, USA) were used for clone and expression of recombinant PcrV protein (r-PcrV), respectively. .. Luria-Bertani (LB) broth, agar (Merck Co., Germany) and Pseudomonas Selective Agar were used for the bacterial culturing.

    Article Title: Localization of a Class III Myosin to Filopodia Tips in Transfected HeLa Cells Requires an Actin-binding Site in its Tail Domain
    Article Snippet: .. Recombinant GST proteins were expressed from pGEX (Pharmacia) vectors in BL21[DE3] E. coli cell line and purified using glutathione-agarose beads (Sigma, St. Louis, MO) per manufacturers protocol. .. Actin filaments were formed in vitro from mammalian actin monomers as described ( ).

    Plasmid Preparation:

    Article Title: Fatty acid DSF binds and allosterically activates histidine kinase RpfC of phytopathogenic bacterium Xanthomonas campestris pv. campestris to regulate quorum-sensing and virulence
    Article Snippet: .. E . coli BL21(DE3) strain was used as the host for expressing recombinant proteins with pET30a vector (Novagen, USA). ..

    Article Title: Characterization of gene expression on genomic segment 7 of infectious salmon anaemia virus
    Article Snippet: .. The plasmid constructs were used to transform competent E. coli strain BL21(DE3)(pLysS) (Novagen) in order to express the GST fusion proteins. ..

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  • 99
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    Millipore e coli strain bl21 rosetta cells
    E Coli Strain Bl21 Rosetta Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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