coli bl21 de3 rosetta gami 2  (Millipore)


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    Structured Review

    Millipore coli bl21 de3 rosetta gami 2
    Coli Bl21 De3 Rosetta Gami 2, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli bl21 de3 rosetta gami 2/product/Millipore
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    coli bl21 de3 rosetta gami 2 - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: Recombinant protein expression and purification Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. .. The cells were harvested by centrifugation at 5000 g for 30 minutes at 4°C and were resuspended in buffer A (50 mM sodium phosphate pH 7.8, 300 mM sodium chloride, 10% glycerol) along with a protease inhibitor tablet (Roche).

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. .. The cells were harvested by centrifugation at 5000 g for 30 minutes at 4°C and were resuspended in buffer A (50 mM sodium phosphate pH 7.8, 300 mM sodium chloride, 10% glycerol) along with a protease inhibitor tablet (Roche).

    Protease Inhibitor:

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: Recombinant protein expression and purification Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. .. The cells were harvested by centrifugation at 5000 g for 30 minutes at 4°C and were resuspended in buffer A (50 mM sodium phosphate pH 7.8, 300 mM sodium chloride, 10% glycerol) along with a protease inhibitor tablet (Roche).

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. .. The cells were harvested by centrifugation at 5000 g for 30 minutes at 4°C and were resuspended in buffer A (50 mM sodium phosphate pH 7.8, 300 mM sodium chloride, 10% glycerol) along with a protease inhibitor tablet (Roche).

    Purification:

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: .. Recombinant protein expression and purification Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. ..

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: Paragraph title: Recombinant protein expression and purification ... Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol.

    Incubation:

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: Recombinant protein expression and purification Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. .. OD600 was measured until it reached 0.6–0.8, at which point expression was induced with 1 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and the culture incubated overnight at 25°C.

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. .. OD600 was measured until it reached 0.6–0.8, at which point expression was induced with 1 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and the culture incubated overnight at 25°C.

    Expressing:

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: .. Recombinant protein expression and purification Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. ..

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: Paragraph title: Recombinant protein expression and purification ... Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol.

    Transformation Assay:

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: .. Recombinant protein expression and purification Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. ..

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: .. Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. ..

    Recombinant:

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: .. Recombinant protein expression and purification Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. ..

    Article Title: Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles
    Article Snippet: .. Recombinant TbALDH3 protein was obtained by cultivating freshly transformed E . coli BL21 (DE3) Rosetta Gami 2 (Novagen) in a starter culture of Luria-Bertani (LB) medium in 20 mL, supplemented with 50 μg/mL of kanamycin and 15 μg/mL of chloramphenicol. ..

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    Millipore bl21 de3 rosetta gami 2 e coli
    Bl21 De3 Rosetta Gami 2 E Coli, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 rosetta gami 2 e coli/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 rosetta gami 2 e coli - by Bioz Stars, 2020-04
    86/100 stars
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