coli bl21 al  (Thermo Fisher)


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  • 90

    Structured Review

    Thermo Fisher coli bl21 al
    Coli Bl21 Al, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli bl21 al/product/Thermo Fisher
    Average 90 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    coli bl21 al - by Bioz Stars, 2020-09
    90/100 stars

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    Recombinant:

    Article Title: IMPIPS: The Immune Protection- Inducing Protein Structure Concept in the Search for Steric-Electron and Topochemical Principles for Complete Fully-Protective Chemically Synthesised Vaccine Development
    Article Snippet: .. All recombinant proteins were expressed in E . coli BL21-Al (Invitrogen), following the manufacturer’s recommendations, purified by affinity chromatography and the fractions were pooled and quantified using a Micro BCA protein assay kit (Thermo Scientific, Meridian, USA). .. Expected protein molecular weight bands were observed in Coomassie blue staining and Western blot (CSP-rI 10.0 kDa, CSP-rII 11.0 kDa, TRAP-rII 8.5 kDa, AMA-1 38 kDa, MSP-2-rI 14.5kDa, EBA-175-rI 26.1 kDa and EBA-175- rII 31.5 kDa).

    Purification:

    Article Title: Application of β-Lactamase Reporter Fusions as an Indicator of Effector Protein Secretion during Infections with the Obligate Intracellular Pathogen Chlamydia trachomatis
    Article Snippet: .. His-Tagged CT695 was expressed in E . coli BL21-Al (Invitrogen), and protein was purified to homogeneity via passage of lysates over TALON affinity resin (Clontech, Mountain View, CA). ..

    Article Title: IMPIPS: The Immune Protection- Inducing Protein Structure Concept in the Search for Steric-Electron and Topochemical Principles for Complete Fully-Protective Chemically Synthesised Vaccine Development
    Article Snippet: .. All recombinant proteins were expressed in E . coli BL21-Al (Invitrogen), following the manufacturer’s recommendations, purified by affinity chromatography and the fractions were pooled and quantified using a Micro BCA protein assay kit (Thermo Scientific, Meridian, USA). .. Expected protein molecular weight bands were observed in Coomassie blue staining and Western blot (CSP-rI 10.0 kDa, CSP-rII 11.0 kDa, TRAP-rII 8.5 kDa, AMA-1 38 kDa, MSP-2-rI 14.5kDa, EBA-175-rI 26.1 kDa and EBA-175- rII 31.5 kDa).

    Affinity Chromatography:

    Article Title: IMPIPS: The Immune Protection- Inducing Protein Structure Concept in the Search for Steric-Electron and Topochemical Principles for Complete Fully-Protective Chemically Synthesised Vaccine Development
    Article Snippet: .. All recombinant proteins were expressed in E . coli BL21-Al (Invitrogen), following the manufacturer’s recommendations, purified by affinity chromatography and the fractions were pooled and quantified using a Micro BCA protein assay kit (Thermo Scientific, Meridian, USA). .. Expected protein molecular weight bands were observed in Coomassie blue staining and Western blot (CSP-rI 10.0 kDa, CSP-rII 11.0 kDa, TRAP-rII 8.5 kDa, AMA-1 38 kDa, MSP-2-rI 14.5kDa, EBA-175-rI 26.1 kDa and EBA-175- rII 31.5 kDa).

    BIA-KA:

    Article Title: IMPIPS: The Immune Protection- Inducing Protein Structure Concept in the Search for Steric-Electron and Topochemical Principles for Complete Fully-Protective Chemically Synthesised Vaccine Development
    Article Snippet: .. All recombinant proteins were expressed in E . coli BL21-Al (Invitrogen), following the manufacturer’s recommendations, purified by affinity chromatography and the fractions were pooled and quantified using a Micro BCA protein assay kit (Thermo Scientific, Meridian, USA). .. Expected protein molecular weight bands were observed in Coomassie blue staining and Western blot (CSP-rI 10.0 kDa, CSP-rII 11.0 kDa, TRAP-rII 8.5 kDa, AMA-1 38 kDa, MSP-2-rI 14.5kDa, EBA-175-rI 26.1 kDa and EBA-175- rII 31.5 kDa).

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    ge healthcare competent bl21 e coli cells
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    Thermo Fisher bl21 cells
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