Structured Review

GenScript codon optimized version
Codon Optimized Version, supplied by GenScript, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/codon optimized version/product/GenScript
Average 91 stars, based on 26 article reviews
Price from $9.99 to $1999.99
codon optimized version - by Bioz Stars, 2020-09
91/100 stars

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Related Articles

MTT Assay:

Article Title: Impact of Extrinsic and Intrinsic Hypoxia on Catecholamine Biosynthesis in Absence or Presence of Hif2α in Pheochromocytoma Cells
Article Snippet: .. These cells were transfected with pcDNA3.1+ carrying a codon-optimized version of the murine Epas1 gene (MTT H2A; Genescript, Piscataway, NJ, USA) via nucleofection (4D-Nucleofector™ System, Lonza). .. At the same time, an empty-vector control cell line was generated (MTT control).

Transfection:

Article Title: Impact of Extrinsic and Intrinsic Hypoxia on Catecholamine Biosynthesis in Absence or Presence of Hif2α in Pheochromocytoma Cells
Article Snippet: .. These cells were transfected with pcDNA3.1+ carrying a codon-optimized version of the murine Epas1 gene (MTT H2A; Genescript, Piscataway, NJ, USA) via nucleofection (4D-Nucleofector™ System, Lonza). .. At the same time, an empty-vector control cell line was generated (MTT control).

Positron Emission Tomography:

Article Title: Structure and function of Bs164 β-mannosidase from Bacteroides salyersiae the founding member of glycoside hydrolase family GH164
Article Snippet: .. A codon optimized version of this gene with a His6 tag in place of the signal peptide (MGSSHHHHHHSSGLEVLFQGPA) was synthesized by and cloned into a pET-28 vector by GenScript (Leiden, Netherlands). .. Plasmid was transformed into chemically competent BL21(DE3 ) gold cells (Agilent) and plated on LB agar containing 50 μg/ml of kanamycin.

Clone Assay:

Article Title: Structure and function of Bs164 β-mannosidase from Bacteroides salyersiae the founding member of glycoside hydrolase family GH164
Article Snippet: .. A codon optimized version of this gene with a His6 tag in place of the signal peptide (MGSSHHHHHHSSGLEVLFQGPA) was synthesized by and cloned into a pET-28 vector by GenScript (Leiden, Netherlands). .. Plasmid was transformed into chemically competent BL21(DE3 ) gold cells (Agilent) and plated on LB agar containing 50 μg/ml of kanamycin.

Article Title: Analysis of Chloroquine Resistance Transporter (CRT) Isoforms and Orthologues in S. cerevisiae Yeast
Article Snippet: .. A codon-optimized version [ ] of the wild type P. falciparum chloroquine resistance transporter ( pfcrt ) cDNA sequence, designed with a 5′-Kozak sequence and both V5 and polyhistidine C-terminal epitope tags, was synthesized by Genescript (Piscataway, NJ) and cloned into vector pUC57 to create pUC57/PfHB3vh. .. The PfHB3vh fragment was restricted from pUC57/PfHB3vh with Kpn1 and EcoRI and subcloned behind the GAL1 promoter of Kpn1/EcoRI-digested plasmid pYES2 (kindly provided by Dr. William Fonzi, Georgetown University) to create pYES2/PfHB3vh.

Article Title: Cloning and expression of selected ABC transporters from the Arabidopsis thaliana ABCG family in Pichia pastoris
Article Snippet: .. The e GFP gene was synthesized as a codon optimized version for P . pastoris and cloned into pSGP18-PDR2-Ntag and pSGP18-PDR8-Ntag by Genscript. .. In order to generate the construct for expression of free eGFP a stop codon was integrated downstream of the eGFP coding sequence by site directed mutagenesis PCR.

Article Title: A Homology Model Reveals Novel Structural Features and an Immunodominant Surface Loop/Opsonic Target in the Treponema pallidum BamA Ortholog TP_0326
Article Snippet: .. The β-barrel region of TP_0326 (residues 434 through 837) was PCR amplified from the codon-optimized version of TP_0326 (synthesized by GeneScript) and cloned into the NheI (5′-end) and HindIII (3′-end) sites of pET23b in frame with the C-terminal polyhistidine. .. The skp gene was PCR amplified from E. coli DNA and cloned into the NheI (5′-end) and XhoI (3′-end) restriction sites of pET23b (Novagen, San Diego, CA) in frame with the C-terminal polyhistidine.

Synthesized:

Article Title: Structure and function of Bs164 β-mannosidase from Bacteroides salyersiae the founding member of glycoside hydrolase family GH164
Article Snippet: .. A codon optimized version of this gene with a His6 tag in place of the signal peptide (MGSSHHHHHHSSGLEVLFQGPA) was synthesized by and cloned into a pET-28 vector by GenScript (Leiden, Netherlands). .. Plasmid was transformed into chemically competent BL21(DE3 ) gold cells (Agilent) and plated on LB agar containing 50 μg/ml of kanamycin.

Article Title: Analysis of Chloroquine Resistance Transporter (CRT) Isoforms and Orthologues in S. cerevisiae Yeast
Article Snippet: .. A codon-optimized version [ ] of the wild type P. falciparum chloroquine resistance transporter ( pfcrt ) cDNA sequence, designed with a 5′-Kozak sequence and both V5 and polyhistidine C-terminal epitope tags, was synthesized by Genescript (Piscataway, NJ) and cloned into vector pUC57 to create pUC57/PfHB3vh. .. The PfHB3vh fragment was restricted from pUC57/PfHB3vh with Kpn1 and EcoRI and subcloned behind the GAL1 promoter of Kpn1/EcoRI-digested plasmid pYES2 (kindly provided by Dr. William Fonzi, Georgetown University) to create pYES2/PfHB3vh.

Article Title: Cloning and expression of selected ABC transporters from the Arabidopsis thaliana ABCG family in Pichia pastoris
Article Snippet: .. The e GFP gene was synthesized as a codon optimized version for P . pastoris and cloned into pSGP18-PDR2-Ntag and pSGP18-PDR8-Ntag by Genscript. .. In order to generate the construct for expression of free eGFP a stop codon was integrated downstream of the eGFP coding sequence by site directed mutagenesis PCR.

Article Title: Identification and Functional Analysis of the Primary Pantothenate Transporter, PfPAT, of the Human Malaria Parasite Plasmodium falciparum *
Article Snippet: .. A codon-optimized version of PfPAT ( PfPAT co ) was synthesized to improve the codon usage and decrease the A + T content (Genscript). .. PfPAT was inserted into the yeast expression vector pYES2.1-/V5-His-TOPO (Invitrogen) generating pYES-PfPATco -V5.

Article Title: Structural Basis for Properdin Oligomerization and Convertase Stimulation in the Human Complement System
Article Snippet: .. A codon optimized version of the miniFH construct similar to that in Schmidt et al. ( ) was synthesized (GenScript) with a C-terminal TEV-site followed by a 6His-tag and inserted into pcDNA3.1. .. MiniFH was expressed in HEK293F cells after PEI transfection and purified by affinity chromatography followed by anion-exchange on a 1 mL Source15Q column equilibrated in 20 mM Tris pH 8.8.

Article Title: A Homology Model Reveals Novel Structural Features and an Immunodominant Surface Loop/Opsonic Target in the Treponema pallidum BamA Ortholog TP_0326
Article Snippet: .. The β-barrel region of TP_0326 (residues 434 through 837) was PCR amplified from the codon-optimized version of TP_0326 (synthesized by GeneScript) and cloned into the NheI (5′-end) and HindIII (3′-end) sites of pET23b in frame with the C-terminal polyhistidine. .. The skp gene was PCR amplified from E. coli DNA and cloned into the NheI (5′-end) and XhoI (3′-end) restriction sites of pET23b (Novagen, San Diego, CA) in frame with the C-terminal polyhistidine.

Article Title: Argonaute-mediated system for supersensitive and multiplexed detection of rare mutations
Article Snippet: .. Pf Ago Expression and Purification A codon-optimized version of the Pf Ago gene was synthesized by GenScript (Nanjing, China), which was designed as the pET28a-derived plasmid pEX-Pf Ago with an N-terminal His-tag. .. The expression plasmid was transformed into E. coli BL21(DE3) cells.

Construct:

Article Title: Structural Basis for Properdin Oligomerization and Convertase Stimulation in the Human Complement System
Article Snippet: .. A codon optimized version of the miniFH construct similar to that in Schmidt et al. ( ) was synthesized (GenScript) with a C-terminal TEV-site followed by a 6His-tag and inserted into pcDNA3.1. .. MiniFH was expressed in HEK293F cells after PEI transfection and purified by affinity chromatography followed by anion-exchange on a 1 mL Source15Q column equilibrated in 20 mM Tris pH 8.8.

Purification:

Article Title: Argonaute-mediated system for supersensitive and multiplexed detection of rare mutations
Article Snippet: .. Pf Ago Expression and Purification A codon-optimized version of the Pf Ago gene was synthesized by GenScript (Nanjing, China), which was designed as the pET28a-derived plasmid pEX-Pf Ago with an N-terminal His-tag. .. The expression plasmid was transformed into E. coli BL21(DE3) cells.

Sequencing:

Article Title: Analysis of Chloroquine Resistance Transporter (CRT) Isoforms and Orthologues in S. cerevisiae Yeast
Article Snippet: .. A codon-optimized version [ ] of the wild type P. falciparum chloroquine resistance transporter ( pfcrt ) cDNA sequence, designed with a 5′-Kozak sequence and both V5 and polyhistidine C-terminal epitope tags, was synthesized by Genescript (Piscataway, NJ) and cloned into vector pUC57 to create pUC57/PfHB3vh. .. The PfHB3vh fragment was restricted from pUC57/PfHB3vh with Kpn1 and EcoRI and subcloned behind the GAL1 promoter of Kpn1/EcoRI-digested plasmid pYES2 (kindly provided by Dr. William Fonzi, Georgetown University) to create pYES2/PfHB3vh.

Amplification:

Article Title: A Homology Model Reveals Novel Structural Features and an Immunodominant Surface Loop/Opsonic Target in the Treponema pallidum BamA Ortholog TP_0326
Article Snippet: .. The β-barrel region of TP_0326 (residues 434 through 837) was PCR amplified from the codon-optimized version of TP_0326 (synthesized by GeneScript) and cloned into the NheI (5′-end) and HindIII (3′-end) sites of pET23b in frame with the C-terminal polyhistidine. .. The skp gene was PCR amplified from E. coli DNA and cloned into the NheI (5′-end) and XhoI (3′-end) restriction sites of pET23b (Novagen, San Diego, CA) in frame with the C-terminal polyhistidine.

Expressing:

Article Title: Argonaute-mediated system for supersensitive and multiplexed detection of rare mutations
Article Snippet: .. Pf Ago Expression and Purification A codon-optimized version of the Pf Ago gene was synthesized by GenScript (Nanjing, China), which was designed as the pET28a-derived plasmid pEX-Pf Ago with an N-terminal His-tag. .. The expression plasmid was transformed into E. coli BL21(DE3) cells.

Polymerase Chain Reaction:

Article Title: A Homology Model Reveals Novel Structural Features and an Immunodominant Surface Loop/Opsonic Target in the Treponema pallidum BamA Ortholog TP_0326
Article Snippet: .. The β-barrel region of TP_0326 (residues 434 through 837) was PCR amplified from the codon-optimized version of TP_0326 (synthesized by GeneScript) and cloned into the NheI (5′-end) and HindIII (3′-end) sites of pET23b in frame with the C-terminal polyhistidine. .. The skp gene was PCR amplified from E. coli DNA and cloned into the NheI (5′-end) and XhoI (3′-end) restriction sites of pET23b (Novagen, San Diego, CA) in frame with the C-terminal polyhistidine.

Plasmid Preparation:

Article Title: Structure and function of Bs164 β-mannosidase from Bacteroides salyersiae the founding member of glycoside hydrolase family GH164
Article Snippet: .. A codon optimized version of this gene with a His6 tag in place of the signal peptide (MGSSHHHHHHSSGLEVLFQGPA) was synthesized by and cloned into a pET-28 vector by GenScript (Leiden, Netherlands). .. Plasmid was transformed into chemically competent BL21(DE3 ) gold cells (Agilent) and plated on LB agar containing 50 μg/ml of kanamycin.

Article Title: Analysis of Chloroquine Resistance Transporter (CRT) Isoforms and Orthologues in S. cerevisiae Yeast
Article Snippet: .. A codon-optimized version [ ] of the wild type P. falciparum chloroquine resistance transporter ( pfcrt ) cDNA sequence, designed with a 5′-Kozak sequence and both V5 and polyhistidine C-terminal epitope tags, was synthesized by Genescript (Piscataway, NJ) and cloned into vector pUC57 to create pUC57/PfHB3vh. .. The PfHB3vh fragment was restricted from pUC57/PfHB3vh with Kpn1 and EcoRI and subcloned behind the GAL1 promoter of Kpn1/EcoRI-digested plasmid pYES2 (kindly provided by Dr. William Fonzi, Georgetown University) to create pYES2/PfHB3vh.

Article Title: Argonaute-mediated system for supersensitive and multiplexed detection of rare mutations
Article Snippet: .. Pf Ago Expression and Purification A codon-optimized version of the Pf Ago gene was synthesized by GenScript (Nanjing, China), which was designed as the pET28a-derived plasmid pEX-Pf Ago with an N-terminal His-tag. .. The expression plasmid was transformed into E. coli BL21(DE3) cells.

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    GenScript codon optimised version
    Codon Optimised Version, supplied by GenScript, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/codon optimised version/product/GenScript
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    codon optimised version - by Bioz Stars, 2020-09
    90/100 stars
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