coating nunctm flat bottom 96 well plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher coating nunctm flat bottom 96 well plates
    Coating Nunctm Flat Bottom 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coating nunctm flat bottom 96 well plates/product/Thermo Fisher
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    coating nunctm flat bottom 96 well plates - by Bioz Stars, 2020-04
    96/100 stars

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    Recombinant:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: .. The ELISA assays on coated chimeric protein were performed by coating NuncTM flat-bottom 96-well plates (Thermo Fisher Scientific, 3596) with 5 μg/mL rhPD-1, rhPD-L1 and rhLAG-3 recombinant proteins in a solution of 0.05 M NaHCO3 for 72 hours at 37°C. .. After blocking of the coated 96-well plates with 5% nonfat dry milk in PBS for 1 hour at 37°C, the purified mAbs were added at increasing concentrations (10–200 nM) to the plates in 2.5% nonfat dry milk in PBS and incubated for 2 hours at room temperature by gently shaking.

    Multiple Displacement Amplification:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Enzyme-linked immunosorbent assay To confirm the binding specificity of the purified mAbs, ELISA assays were performed on chimeric proteins (coated at 5 μg/mL on microplates), tumor cells (PD-L1-positive breast cancer MDA-MB-231 cells or PD-L1-negative breast cancer MCF7 cells), and untreated or activated hPBMCs. .. The ELISA assays on coated chimeric protein were performed by coating NuncTM flat-bottom 96-well plates (Thermo Fisher Scientific, 3596) with 5 μg/mL rhPD-1, rhPD-L1 and rhLAG-3 recombinant proteins in a solution of 0.05 M NaHCO3 for 72 hours at 37°C.

    Blocking Assay:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: The ELISA assays on coated chimeric protein were performed by coating NuncTM flat-bottom 96-well plates (Thermo Fisher Scientific, 3596) with 5 μg/mL rhPD-1, rhPD-L1 and rhLAG-3 recombinant proteins in a solution of 0.05 M NaHCO3 for 72 hours at 37°C. .. After blocking of the coated 96-well plates with 5% nonfat dry milk in PBS for 1 hour at 37°C, the purified mAbs were added at increasing concentrations (10–200 nM) to the plates in 2.5% nonfat dry milk in PBS and incubated for 2 hours at room temperature by gently shaking.

    Purification:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Enzyme-linked immunosorbent assay To confirm the binding specificity of the purified mAbs, ELISA assays were performed on chimeric proteins (coated at 5 μg/mL on microplates), tumor cells (PD-L1-positive breast cancer MDA-MB-231 cells or PD-L1-negative breast cancer MCF7 cells), and untreated or activated hPBMCs. .. The ELISA assays on coated chimeric protein were performed by coating NuncTM flat-bottom 96-well plates (Thermo Fisher Scientific, 3596) with 5 μg/mL rhPD-1, rhPD-L1 and rhLAG-3 recombinant proteins in a solution of 0.05 M NaHCO3 for 72 hours at 37°C.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: .. The ELISA assays on coated chimeric protein were performed by coating NuncTM flat-bottom 96-well plates (Thermo Fisher Scientific, 3596) with 5 μg/mL rhPD-1, rhPD-L1 and rhLAG-3 recombinant proteins in a solution of 0.05 M NaHCO3 for 72 hours at 37°C. .. After blocking of the coated 96-well plates with 5% nonfat dry milk in PBS for 1 hour at 37°C, the purified mAbs were added at increasing concentrations (10–200 nM) to the plates in 2.5% nonfat dry milk in PBS and incubated for 2 hours at room temperature by gently shaking.

    Incubation:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: The ELISA assays on coated chimeric protein were performed by coating NuncTM flat-bottom 96-well plates (Thermo Fisher Scientific, 3596) with 5 μg/mL rhPD-1, rhPD-L1 and rhLAG-3 recombinant proteins in a solution of 0.05 M NaHCO3 for 72 hours at 37°C. .. After blocking of the coated 96-well plates with 5% nonfat dry milk in PBS for 1 hour at 37°C, the purified mAbs were added at increasing concentrations (10–200 nM) to the plates in 2.5% nonfat dry milk in PBS and incubated for 2 hours at room temperature by gently shaking.

    Binding Assay:

    Article Title: Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
    Article Snippet: Enzyme-linked immunosorbent assay To confirm the binding specificity of the purified mAbs, ELISA assays were performed on chimeric proteins (coated at 5 μg/mL on microplates), tumor cells (PD-L1-positive breast cancer MDA-MB-231 cells or PD-L1-negative breast cancer MCF7 cells), and untreated or activated hPBMCs. .. The ELISA assays on coated chimeric protein were performed by coating NuncTM flat-bottom 96-well plates (Thermo Fisher Scientific, 3596) with 5 μg/mL rhPD-1, rhPD-L1 and rhLAG-3 recombinant proteins in a solution of 0.05 M NaHCO3 for 72 hours at 37°C.

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  • 96
    Thermo Fisher coating nunctm flat bottom 96 well plates
    Coating Nunctm Flat Bottom 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coating nunctm flat bottom 96 well plates/product/Thermo Fisher
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    coating nunctm flat bottom 96 well plates - by Bioz Stars, 2020-04
    96/100 stars
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