co2 water jacketed incubator  (Thermo Fisher)


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    Structured Review

    Thermo Fisher co2 water jacketed incubator
    Co2 Water Jacketed Incubator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co2 water jacketed incubator/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    co2 water jacketed incubator - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells
    Article Snippet: The cell lines grown at 37°C Forma Water-Jacketed CO2 incubator (Thermo Fisher Scientific Inc., Waltham, MA) with a humidified 5% CO2 /95% air were free of mycoplasma infection. .. HEK293F and HeLa stable cell clones or transiently transfected HEK293F cells were exposed to UVB radiation, as described previously (Kim et al., ).

    Stable Transfection:

    Article Title: DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells
    Article Snippet: The cell lines grown at 37°C Forma Water-Jacketed CO2 incubator (Thermo Fisher Scientific Inc., Waltham, MA) with a humidified 5% CO2 /95% air were free of mycoplasma infection. .. HEK293F and HeLa stable cell clones or transiently transfected HEK293F cells were exposed to UVB radiation, as described previously (Kim et al., ).

    Autoradiography:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Cytometry:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Real-time Polymerase Chain Reaction:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Incubation:

    Article Title: Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
    Article Snippet: .. The cells were incubated in 25 cm2 sealed vials [35-3108, Becton Dickinson FalconTM , NJ, USA] with 3.5 mL of medium that contains RPMI medium 1640 (11875-093, Gibco-BRL, Gaithersburg, MD, USA) with 2% antibiotic/antimycotic (15240-095, Gibco-BRL), 1% hepes buffer (15630-105, Gibco-BRL), and also supplemented with 10% Fetal Bovine Serum (FBS) (10437-010, Gibco-BRL) at 37 ºC in a water jacketed CO2 incubator (Forma Series II, Thermo Fisher Scientific Inc, Waltham, MA, USA). .. Experimental preparation Cells were removed from the original culture vials using 0.5% of 10X Trypsin-EDTA (15400-054, Gibco-BRL), and then seeded on a 25 mm glass coverslip (pre-washed with 99.9% ethanol (34964, Sigma Aldrich) to remove any possible coatings that may affect cell attachment and growth) in culture medium in a 35 × 10 mm dish.

    Article Title: Hypoxia Regulates mTORC1-Mediated Keratinocyte Motility and Migration via the AMPK Pathway
    Article Snippet: Briefly, keratinocytes were obtained from the skin by incubation with a 0.25% trypsin/0.04% EDTA solution (Invitrogen, USA) at 4°C overnight and were then plated into collagen type IV-coated dishes. .. A Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which maintains a desired and precise culturing environment, kept CO2 at 5% and O2 at 2% by nitrogen displacement.

    Article Title: Involvement of autophagy in hypoxia-BNIP3 signaling to promote epidermal keratinocyte migration
    Article Snippet: Hypoxia exposure Hypoxic conditions of 2% O2 , 5% CO2 and 93% N2 were created by a constant flow of nitrogen using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which maintained a precise culturing environment (37 °C) and desired O2 level. .. A p38 inhibitor (SB203580, Invitrogen, 5 μM), JNK inhibitor (SP600125, Beyotime, 5 μM), and an antioxidant (NAC, Sigma-Aldrich, 5 mM) were respectively added to the cultures and incubated at 37 °C for 30 min before hypoxic treatment.

    Article Title: Microtubule-associated protein 4 phosphorylation regulates epidermal keratinocyte migration and proliferation
    Article Snippet: Hypoxia exposure Hypoxic conditions of 2% O2 , 5% CO2 and 93% N2 were created by a constant flow of nitrogen using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which maintained the desired temperature (37 °C) and O2 level. .. A p38 inhibitor (SB203580, Invitrogen, 5 μM) was added to the cultures and incubated at 37 °C for 30 min prior to hypoxic treatment.

    Cell Culture:

    Article Title: Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
    Article Snippet: Paragraph title: Cell culture ... The cells were incubated in 25 cm2 sealed vials [35-3108, Becton Dickinson FalconTM , NJ, USA] with 3.5 mL of medium that contains RPMI medium 1640 (11875-093, Gibco-BRL, Gaithersburg, MD, USA) with 2% antibiotic/antimycotic (15240-095, Gibco-BRL), 1% hepes buffer (15630-105, Gibco-BRL), and also supplemented with 10% Fetal Bovine Serum (FBS) (10437-010, Gibco-BRL) at 37 ºC in a water jacketed CO2 incubator (Forma Series II, Thermo Fisher Scientific Inc, Waltham, MA, USA).

    Article Title: Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
    Article Snippet: Paragraph title: Cell culture and hypoxia treatment ... Hypoxic conditions of 2% O2 , 5% CO2 and 93% N2 were created by a continuous flow of nitrogen by using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific).

    Article Title: DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells
    Article Snippet: Cell cultures and UVB irradiation HEK293F (Life Technologies) and RetroPackTM PT-67 cell lines (Clontech) were cultured in the Dulbecco’s modified Eagle’s medium (D-MEM) supplemented with 10% fetal bovine serum (FBS), 2 mmol/L glutamine, 100 units/mL penicillin, 100 μg/mL streptomycin sulfate, and 1 mmol/L sodium pyruvate (Life Technologies). .. The cell lines grown at 37°C Forma Water-Jacketed CO2 incubator (Thermo Fisher Scientific Inc., Waltham, MA) with a humidified 5% CO2 /95% air were free of mycoplasma infection.

    Article Title: Hypoxia Regulates mTORC1-Mediated Keratinocyte Motility and Migration via the AMPK Pathway
    Article Snippet: Paragraph title: Cell culture and hypoxia treatment ... A Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which maintains a desired and precise culturing environment, kept CO2 at 5% and O2 at 2% by nitrogen displacement.

    Article Title: Anti-EGFR-Conjugated Hollow Gold Nanospheres Enhance Radiocytotoxic Targeting of Cervical Cancer at Megavoltage Radiation Energies
    Article Snippet: .. Cell Culture Human cervical cancer cell line HeLa, obtained from the Gynecological Tumor Laboratory of Qilu Hospital of Shandong University, was used in the experiment, which was cultured in RPMI Medium 1640 (GIBCO, Invitrogen Corporation) supplemented with 10 % fetal bovine serum (FBS) (Kangyuan Biology, China) and 1 % penicillin–streptomycin (Solarbio, Beijing Solarbio Science & Technology, China) in water-jacketed CO2 incubators (Thermo Fisher Scientific Forma Series II, USA) at 37 °C with 95 % (v /v ) air and 5 % (v /v ) CO2 . ..

    Modification:

    Article Title: DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells
    Article Snippet: Cell cultures and UVB irradiation HEK293F (Life Technologies) and RetroPackTM PT-67 cell lines (Clontech) were cultured in the Dulbecco’s modified Eagle’s medium (D-MEM) supplemented with 10% fetal bovine serum (FBS), 2 mmol/L glutamine, 100 units/mL penicillin, 100 μg/mL streptomycin sulfate, and 1 mmol/L sodium pyruvate (Life Technologies). .. The cell lines grown at 37°C Forma Water-Jacketed CO2 incubator (Thermo Fisher Scientific Inc., Waltham, MA) with a humidified 5% CO2 /95% air were free of mycoplasma infection.

    Over Expression:

    Article Title: Role of Ran-regulated nuclear-cytoplasmic trafficking of pVHL in the regulation of microtubular stability-mediated HIF-1α in hypoxic cardiomyocytes
    Article Snippet: Hypoxia treatment and microtubule interference Hypoxic conditions of 1% O2 , 5% CO2 , and 94% N2 were created by a continuous flow of nitrogen using a Forma Series II Water Jacket CO2 incubator (model 3131; Thermo Scientific, USA). .. Microtubule stabilization was achieved by treating the CMs with 10 mM paclitaxel (Sigma, USA) or infecting them with the MAP4 overexpression vector (MAP4-Ad) for 8 h prior to hypoxia treatment.

    Flow Cytometry:

    Article Title: Role of Ran-regulated nuclear-cytoplasmic trafficking of pVHL in the regulation of microtubular stability-mediated HIF-1α in hypoxic cardiomyocytes
    Article Snippet: .. Hypoxia treatment and microtubule interference Hypoxic conditions of 1% O2 , 5% CO2 , and 94% N2 were created by a continuous flow of nitrogen using a Forma Series II Water Jacket CO2 incubator (model 3131; Thermo Scientific, USA). .. The recombinant adenovirus vectors were used to infect CMs before hypoxia treatment.

    Article Title: Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
    Article Snippet: .. Hypoxic conditions of 2% O2 , 5% CO2 and 93% N2 were created by a continuous flow of nitrogen by using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific). .. The p38/MAPK inhibitor, SB203580 (Beyotime) (5 μmol/L), was added to these cultures and allowed to incubated at 37°C for 30 minutes before hypoxia treatment.

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Article Title: Involvement of autophagy in hypoxia-BNIP3 signaling to promote epidermal keratinocyte migration
    Article Snippet: .. Hypoxia exposure Hypoxic conditions of 2% O2 , 5% CO2 and 93% N2 were created by a constant flow of nitrogen using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which maintained a precise culturing environment (37 °C) and desired O2 level. .. A p38 inhibitor (SB203580, Invitrogen, 5 μM), JNK inhibitor (SP600125, Beyotime, 5 μM), and an antioxidant (NAC, Sigma-Aldrich, 5 mM) were respectively added to the cultures and incubated at 37 °C for 30 min before hypoxic treatment.

    Article Title: Microtubule-associated protein 4 phosphorylation regulates epidermal keratinocyte migration and proliferation
    Article Snippet: .. Hypoxia exposure Hypoxic conditions of 2% O2 , 5% CO2 and 93% N2 were created by a constant flow of nitrogen using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which maintained the desired temperature (37 °C) and O2 level. .. A p38 inhibitor (SB203580, Invitrogen, 5 μM) was added to the cultures and incubated at 37 °C for 30 min prior to hypoxic treatment.

    Infection:

    Article Title: DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells
    Article Snippet: .. The cell lines grown at 37°C Forma Water-Jacketed CO2 incubator (Thermo Fisher Scientific Inc., Waltham, MA) with a humidified 5% CO2 /95% air were free of mycoplasma infection. .. HEK293F and HeLa stable cell clones or transiently transfected HEK293F cells were exposed to UVB radiation, as described previously (Kim et al., ).

    Generated:

    Article Title: Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration
    Article Snippet: Hypoxia treatment Hypoxic conditions were created using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), with which we could precisely control oxygen levels and temperature. .. The hypoxic conditions were generated at 37 °C in 5% CO2 and the designated oxygen content was balanced with N2 .

    Article Title: The Galvanotactic Migration of Keratinocytes is Enhanced by Hypoxic Preconditioning
    Article Snippet: Hypoxia pretreatment The hypoxic conditions were created using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which allows precise oxygen and temperature regulation. .. The hypoxic conditions were generated at 37 °C in 5% CO2 and the designated oxygen content, balanced with N2 .

    Imaging:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Polymerase Chain Reaction:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Recombinant:

    Article Title: Role of Ran-regulated nuclear-cytoplasmic trafficking of pVHL in the regulation of microtubular stability-mediated HIF-1α in hypoxic cardiomyocytes
    Article Snippet: Hypoxia treatment and microtubule interference Hypoxic conditions of 1% O2 , 5% CO2 , and 94% N2 were created by a continuous flow of nitrogen using a Forma Series II Water Jacket CO2 incubator (model 3131; Thermo Scientific, USA). .. The recombinant adenovirus vectors were used to infect CMs before hypoxia treatment.

    Nucleic Acid Electrophoresis:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Fluorescence:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Isolation:

    Article Title: Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
    Article Snippet: Briefly, MKs were isolated from skin of newborn BALB/c mice (postnatal day 1–3) by 0.25% trypsin/0.04% EDTA solution (Invitrogen, USA) at 4°C overnight, and primary cultures were established in keritinocyte serum-free medium (K-SFM medium) (Gibco, USA). .. Hypoxic conditions of 2% O2 , 5% CO2 and 93% N2 were created by a continuous flow of nitrogen by using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific).

    Article Title: Hypoxia Regulates mTORC1-Mediated Keratinocyte Motility and Migration via the AMPK Pathway
    Article Snippet: Primary MKs were isolated from the skin of new-born BALB/c mice (postnatal day 1–3) as previously described [ ]. .. A Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which maintains a desired and precise culturing environment, kept CO2 at 5% and O2 at 2% by nitrogen displacement.

    Temperature Regulation:

    Article Title: The Galvanotactic Migration of Keratinocytes is Enhanced by Hypoxic Preconditioning
    Article Snippet: .. Hypoxia pretreatment The hypoxic conditions were created using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which allows precise oxygen and temperature regulation. .. The hypoxic conditions were generated at 37 °C in 5% CO2 and the designated oxygen content, balanced with N2 .

    Transfection:

    Article Title: DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells
    Article Snippet: The cell lines grown at 37°C Forma Water-Jacketed CO2 incubator (Thermo Fisher Scientific Inc., Waltham, MA) with a humidified 5% CO2 /95% air were free of mycoplasma infection. .. HEK293F and HeLa stable cell clones or transiently transfected HEK293F cells were exposed to UVB radiation, as described previously (Kim et al., ).

    Microscopy:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Purification:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    In Vivo Imaging:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Mouse Assay:

    Article Title: Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway
    Article Snippet: Briefly, MKs were isolated from skin of newborn BALB/c mice (postnatal day 1–3) by 0.25% trypsin/0.04% EDTA solution (Invitrogen, USA) at 4°C overnight, and primary cultures were established in keritinocyte serum-free medium (K-SFM medium) (Gibco, USA). .. Hypoxic conditions of 2% O2 , 5% CO2 and 93% N2 were created by a continuous flow of nitrogen by using a Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific).

    Article Title: Hypoxia Regulates mTORC1-Mediated Keratinocyte Motility and Migration via the AMPK Pathway
    Article Snippet: Primary MKs were isolated from the skin of new-born BALB/c mice (postnatal day 1–3) as previously described [ ]. .. A Forma Series II Water Jacket CO2 incubator (model: 3131; Thermo Scientific), which maintains a desired and precise culturing environment, kept CO2 at 5% and O2 at 2% by nitrogen displacement.

    Article Title: LF-MF inhibits iron metabolism and suppresses lung cancer through activation of P53-miR-34a-E2F1/E2F3 pathway
    Article Snippet: Control mice were placed in a similar apparatus except that there were two rotating iron plates instead of magnets, thus lacking a LF-MF. .. For cell experiment, a smaller instrument with similar structure to generate 0.4T and 7.5 Hz LF-MF was installed in Thermo Scientific Forma Series Water-Jacketed CO2 Incubators (Supplementary Fig. ).

    Plasmid Preparation:

    Article Title: Role of Ran-regulated nuclear-cytoplasmic trafficking of pVHL in the regulation of microtubular stability-mediated HIF-1α in hypoxic cardiomyocytes
    Article Snippet: Hypoxia treatment and microtubule interference Hypoxic conditions of 1% O2 , 5% CO2 , and 94% N2 were created by a continuous flow of nitrogen using a Forma Series II Water Jacket CO2 incubator (model 3131; Thermo Scientific, USA). .. Microtubule stabilization was achieved by treating the CMs with 10 mM paclitaxel (Sigma, USA) or infecting them with the MAP4 overexpression vector (MAP4-Ad) for 8 h prior to hypoxia treatment.

    Software:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Irradiation:

    Article Title: DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells
    Article Snippet: Paragraph title: Cell cultures and UVB irradiation ... The cell lines grown at 37°C Forma Water-Jacketed CO2 incubator (Thermo Fisher Scientific Inc., Waltham, MA) with a humidified 5% CO2 /95% air were free of mycoplasma infection.

    Agarose Gel Electrophoresis:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Spectrophotometry:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Thin Layer Chromatography:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

    Hood:

    Article Title: Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells
    Article Snippet: .. Freezer (−80 °C and −20 °C) and 4 °C refrigerator Milli-Q water purification system PCR machine (Eppendorf Mastercycler Gradient, model 5331) Real-time PCR detection system (Roche LightCycler 480 real-time PCR System) NanoDrop 2000 spectrophotometer (Themo Scientific) pH meter (Fisher, Dual channel pH/ion meter AR25) Eppendorf centrifuge 5424 (Eppendorf) Agarose gel electrophoresis system (Biorad, mini-sub cell GT system) PAGE gel electrophoresis system (Hoefer, SE250 mighty small II for 8 × 7 cm gel) TGGE system (Biometra, model 024-000) Eagle eye II imaging system (Stratagen) Fluorescence microscope (Olympus) Optical microscope (Motic company, cat. no. SFC-11) Synergy™ 4 microplate reader (BioTek) Flow cytometer (Beckman) Fluorolog fluorospectrometer (Horiba Jobin Yvon) Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss) Trans-Blot® SD semi-dry transfer cell (Bio-Rad) Biosafety cabinet (Thermo Scientific, model 1307) Forma series II water jacket CO2 incubator (Thermo Scientific) Typhoon FLA 7000 imaging system (GE Healthcare) Hand hold Mineralight® lamp (UVP, model UVGL-25) Flexible TLC plates (Selecto Scientific, cat no. 11078) SAVANT DNA120 Speed Vac concentrator (Themo Electron Corporation) NucAway™ spin columns (Ambion, cat no. AM10070) Microfluro 1 96-well white Microtiter plates (Themo Scientific, cat no. 14-245-194A) Immun-Blot™ PVDF membrane for protein blotting (Bio-rad, cat no. 162-0177) Premium autoradiography film (Deville Scientific, cat no. E3012) Cyclone storage phosphor system (Packard) Elutrap electroelution system (Whatman) MultiMode AFM NanoScope IV system (Veeco/Digital Instruments) operating in tapping mode. .. Regular tapping Mode Silicon Probes (Olympus from Asylum Research) Non-contact NSG01_DLC probes (K-Tek Nanotechnology) Mica sheets (green or ruby mica) (Asheville-Schoonmaker Mica Company) DynaPro 99 Dynamic Light Scattering (Wyatt Technology Corporation) Surgery station Syringe (28G1/2), operation scissors and tweezers (Fisher Scientific) IVIS® in vivo imaging system (PerkinElmer Inc.) Mfold ) ) AFM image analysis software (Veeco) ImageJ ).

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    Thermo Fisher 3110 water jacketed co 2 incubator
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    Thermo Fisher series ii water jacket incubator
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    Thermo Fisher series ii water jacketed incubator
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